Compact Dry “Nissui” EC
For Coliform and E.coli 1
P.aeruginosa
E.aerogenes
E.coli
►Medium contains 2 kinds of chromogenic enzyme substrates that develop
red/pink color for coliform and white blue/blue color for E.coli.
►Incubate for 24 hours at 35 ± 2ºC.
Main component : Chromogenic enzyme substrates Magenta-GAL and X-GLUC
Certificates : AOAC (PTM), MicroVal
Storage : Keep at room temperature (1ºC-30ºC)
Shelf life : 18 months after manufacturing
Package : Compact Dry “Nissui” EC 40 plates (Code: 06742)
: Compact Dry “Nissui” EC 240 plates (Code: 06743)
- Compact Dry is manufactured based on Nissui’s original patented technology. -
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Compact Dry Sample
Preparation Preparation
2
Dilution Water Preparation
(Butterfield's phosphate-buffered diluent)
34 g of KH2PO4 and 500 ml of distilled
water. Adjust pH to 7.2 with 1N NaOH.
Autoclave 15 min at 121°C.
1:10 Dilution Preparation
Analyze 50 g from
Open an aluminum pouch.
each sub-sample.
Add 450 ml of
Butterfield's phosphate-
buffered diluent.
Homogenize in a
stomacher for 2 min.
→1:10 Dilution
Further Dilutions (if necessary)
(1:100 Dilution Preparation)
Take out a set of 4 plates.
( Or take 1 – 3 plates you need and keep the pouch sealed to
avoid a quality loss from light and moisture. )
Take 1 ml of the 1:10
dilution.
Add it to 9 ml of
Butterfield's phosphate-
buffered diluent.
Mix well.
Write appropriate information on a →1:100 Dilution
memorandum section. (Not more than 15 min should elapse.)
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Inoculation
3
Incubation
Pipette 1 ml of a sample. Turn over the plate lidded, put them
in an incubator. Incubate for 24
hours at 35 ± 2°C for Compact Dry
EC.
Interpretation &
Colony Fishing
Take off a lid of a plate.
From the backside of the plate,
count the number of colored
colonies appeared in the medium.
Lighting and using a loupe are
useful to count colonies easier.
Inoculate the 1 ml sample to the
middle of a dry sheet.
Preliminary Advice
For user's convenience, “Sample
Preparation” section on the page 2
of this user's manual is made
with reference to FDA's BAM
(Bacteriological Analytical Manual).
Depending on user's needs, other
Put the lid again. official methods can be also used.
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Counting Examples & Precautions
(Print this page without margins using A4 ( 210 x 297 mm ) paper, the pictures
are printed out as the same as the actual Compact Dry ( 77 x 58 ( x 7 ) mm ).
4
Ex1 Ex1 Ex3
〇： E. coli = 6 colonies In case of more than 300 colonies,
the number of colony should be
◇： Coliform = 6 colonies estimated. The average number of
colony in the grid (1 cm2) is counted,
and then the total number of colony is
Ex Lighting from the back
Ex1 calculated by multiplying the average
Ex Lighting from the front number by 20.
Ex2 Ex2 Ex4
〇： E.coli = 29 colonies In case that numerous colonies are
observed, a sample extraction should
◇： Coliform = 30 colonies be diluted appropriately, and be
Δ : the others = 29 inoculated again.
Ex Lighting from the back
Ex2
Ex Lighting from the front
● After opening the aluminum bag, unused plates should be kept in the folded and sealed bag to avoid light and moisture. ● Used plates must be sterilized by autoclaving and disposed
according to a local waste management law. ● In case of foodstuffs containing their own enzymes, there are cases where a whole medium of Compact Dry is stained. ● The pictures in this
manual has colonies of E.coli, E.aerogenes and P.aeruginosa. There may be subtle differences if other strains grow. ● Because of the lighting, there may be subtle differences in vision
between in the pictures and in actual tests.
Further Information: Customer Support Section
TEL: +81-3-5846-5711 E-mail: customer@nissui-pharm.co.jp
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