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Polygonum Glabrum Willd. Leaf Extract Mediated Green Synthesis of Silver Nanoparticles and Their Assessment of Antimicrobial Activity

Polygonum glabrum Willd leaf extract was used to synthesize silver nanoparticles. The reaction solution turned brown, indicating nanoparticle formation, which was confirmed by a UV-Vis absorption peak at 429 nm. The nanoparticles were characterized using techniques like FTIR, fluorescence spectroscopy, and SEM. FTIR revealed that biomolecules in the leaf extract were involved in nanoparticle reduction and capping. SEM showed the nanoparticles were 10-35 nm and spherical. The biogenic silver nanoparticles demonstrated excellent antimicrobial activity against several pathogens.

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60 views1 page

Polygonum Glabrum Willd. Leaf Extract Mediated Green Synthesis of Silver Nanoparticles and Their Assessment of Antimicrobial Activity

Polygonum glabrum Willd leaf extract was used to synthesize silver nanoparticles. The reaction solution turned brown, indicating nanoparticle formation, which was confirmed by a UV-Vis absorption peak at 429 nm. The nanoparticles were characterized using techniques like FTIR, fluorescence spectroscopy, and SEM. FTIR revealed that biomolecules in the leaf extract were involved in nanoparticle reduction and capping. SEM showed the nanoparticles were 10-35 nm and spherical. The biogenic silver nanoparticles demonstrated excellent antimicrobial activity against several pathogens.

Uploaded by

Afrah M
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DOI: 10.3109/1040841X.2014.

912200 Silver nanoparticles 1

Polygonum glabrum Willd. leaf extract mediated green


synthesis of silver nanoparticles and their assessment of
antimicrobial activity
V. K. Rokhade and T.C.Taranath*
P.G.Department of studies in Botany, Environmental Biology Laboratory, Karnatak University, Dharwad-
580003, Karnataka, India.
*Corresponding Author: V. K. Rokhade

Abstract: Green nanotechnology is gaining importance due to the elimination of harmful reagents and
provides effective synthesis of expected products in an economically manner. In the present investigation
aqueous leaf extract of Polygonum glabrum Willd. was used for the synthesis of silver nanoparticles. The
colour of reaction mixture was changes from colouless to brown colour indicates the formation of silver
nanoparticles, further confirmed by characteristic UV-Vis absorption peak at 429 nm. Synthesized
nanoparticles were characterized by FTIR, Fluorescence Spectroscopy and SEM. Fluorescence
Spectroscopy is a powerful tool to study the tertiary structure of proteins. FTIR data revels that
biomolecules involved in the reduction and capping of silver nanoparticles. The SEM result shows the size of
nanoparticles ranges from 10 to 35 nm and are spherical in shape. XRD data showed that crystalline nature
of nanosilver. The biogenic silver nanoparticles showed excellent antimicrobial activity against S. typhi, E.
coli, P. aeruginosa and S.aureus.
Keywords: Silver nanoparticles, Polygonum glabrum Willd, FTIR, Antimicrobial activity

Date of Submission: 20-05-2018 Date of acceptance: 04-06-


2018

I. Introduction
Nanotechnology is referred to the particular technological goal of precisely manipulating atoms and
molecules for fabrication of macroscale products, also now referred to as molecular nanotechnology 1.
Dimensions between approximately 1 and 100 nanometers are known as the nanoscale. The synthesis of
nanoparticles and their characterization is an emerging field of nanotechnology since past two decades due to
their wide applications in the fields of physics, chemistry, biology and medicine. Unusual physical, chemical
and biological properties can emerge in materials at the nanoscale. These properties may differ in important
ways from the properties of bulk materials and single atoms or molecules. Although metal is a poor catalyst
in bulk form, nanosized particles can exhibit excellent catalytic activity due to their relative high surface
area-to- volume ratio and their interface-dominated properties, which significantly differ from those of the
bulk material
2
. Controlled size and composition of nanoparticles are of fundamental interest since they provide solutions
to environmental and technological challenges in the areas of catalysis, solar energy conversion and waste
water treatment. More recently, nanoscale materials has been looked at with interest for synthesis of
advanced materials, energy storage devices, electronic and optical displays, chemical and biosensors, drug
delivery, optical spectroscopy including surface-enhanced raman scattering 3 , detection and diagonostic,
antimicrobial, therapeutics 4,5,6,7, biomedicine, waste water treatment 8 , food industry 9 , Antidiabetic 10, 11,
Antiviral 12 , anticancer13, Anti-inflammatory 14 and antiplasmodial 15 .

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