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AgNPs Synthesis & Antimicrobial Study

The document summarizes research on synthesizing silver nanoparticles (AgNPs) using plant extracts. It discusses using leaf extracts from several plants, including Stigmaphyllon littorale, Sesbania grandiflora, Artemisia nilagirica, Vitis vinifera, and Ocimum sanctum to rapidly form stable AgNPs ranging from 5-42 nm in size. Characterization of the AgNPs was performed using techniques such as UV-Vis spectroscopy, XRD, SEM, TEM, and FTIR, which confirmed the formation, size, and crystalline structure of the AgNPs. Antimicrobial testing demonstrated the AgNPs had potent antibacterial properties against

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Afrah M
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42 views1 page

AgNPs Synthesis & Antimicrobial Study

The document summarizes research on synthesizing silver nanoparticles (AgNPs) using plant extracts. It discusses using leaf extracts from several plants, including Stigmaphyllon littorale, Sesbania grandiflora, Artemisia nilagirica, Vitis vinifera, and Ocimum sanctum to rapidly form stable AgNPs ranging from 5-42 nm in size. Characterization of the AgNPs was performed using techniques such as UV-Vis spectroscopy, XRD, SEM, TEM, and FTIR, which confirmed the formation, size, and crystalline structure of the AgNPs. Antimicrobial testing demonstrated the AgNPs had potent antibacterial properties against

Uploaded by

Afrah M
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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at 20,000 rpm for 20 minutes to obtain the AgNPs powder for was done by water soluble proteins in the

s in the leaf extract, which was


XRD (X-ray diffraction) analysis. The XRD analysis of the confirmed by FTIR analysis. The UV-Vis spectrum of AgNPs
AgNPs showed four distinct diffraction peaks, which can be solu- tions showed a peak at 422 nm. The XRD spectrum
indexed at the angle values of 111, 200, 220 and 240 [19]. showed four distinct diffraction peaks at (2θ) 38.28o, 44.33o,
In synthesis of AgNPs using a domestic microwave with the 64.33o, and 77.53o corresponds to (1 1 1), (2 0 0), (2 2 0) and (3
leaves extract of Stigmaphyllon littorale, the nanoparticles were 1 1) planes of face- centered cubic silver with a lattice parameter
rapidly formed with a stable size of 5-25 nm. Characterization of α =4.08 Å. The antibacterial activity of AgNPs was
was carried out using UV-Vis, Fourier Transform Infrared (FTIR), investigated against Salmonella enterica (gram-negative) and
SEM and TEM analyses. The antimicrobial properties of obtained Staphylococcus aureus (gram-posi- tive) by disc diffusion
AgNPs were evaluated against gram positive and negative method. The results showed potent antibac- terial activity against
bacterial patho- gens (Pseudomonas putida, Escherichia coli, two bacteria.
Bacillus subtilus and Micrococcus luteus). The formed AgNPs The AgNPs were synthesized using the leaf extract of
showed significant anti- microbial properties [20]. Lakshmi tulasi (Ocimum sanctum) as a reducing and stabilizing
Das et al. [21] reported the synthesis of AgNPs using the leaf agent. The absorption spectrum of the AgNPs showed a peak at
extract of Sesbania grandiflora. The reduction of Ag+ to AgNPs 406 nm. The average diameter of the AgNPs was found to be 42
nm. The struc- ture of the synthesized AgNPs was determined by
XRD analysis.

Fig. 2. The SEM images of the synthesized AgNPs using


Artemisia nilagirica leaf extract [17].

Fig. 3. TEM images of silver nanoparticles synthesized by


using Vitis vinifera extract [18].

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