Scribd
Upload
67 views2 pages

Introduction To Microbiology: Safety Rules in The Lab

This document provides an introduction to microbiology and outlines important safety rules and equipment used in microbiology labs. It describes the study of microorganisms like bacteria, viruses, and fungi. Key lab equipment is defined, including burners, loops, slides, pipettes, containers, petri dishes, and incubators. Proper disposal procedures of contaminated materials are also summarized.

Uploaded by

Cbbxvcjkb
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
67 views2 pages

Introduction To Microbiology: Safety Rules in The Lab

This document provides an introduction to microbiology and outlines important safety rules and equipment used in microbiology labs. It describes the study of microorganisms like bacteria, viruses, and fungi. Key lab equipment is defined, including burners, loops, slides, pipettes, containers, petri dishes, and incubators. Proper disposal procedures of contaminated materials are also summarized.

Uploaded by

Cbbxvcjkb
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

Introduction to Microbiology

Safety rules in the lab:

1) Always wear lab coat and gloves inside the lab.

2) Cover any minor cuts on your body.

3) Never eat, drink or smoke in the lab.

4) Never mouth-pipette.

5) Report any accident to your supervisor.

6) Never take any culture out of the lab.

7) Always wash your hands before leaving the lab.

Microbiology: Study of small organisms.

Ex. Bacteria, viruses, fungi and parasites.

Found in: Air, soil, water and in our bodies

Name and function of common items used in the lab:

 Burner: To provide area almost free of organism.

A. Bunsen burner: use flame.

B. Bacti burner: it's electrical.

 Loops: To transfer organism from one place to another (from culture to the slide).

A. Wire loop: large amount.

B. Straight loop: small amount.

The loops are made up of special material called (Nichrome). It heats and get cool rapidly.

 Slide: To place the organism on top of it to examine it under the microscope.

A. Plain slide.

B. Frosted end slide.

 Cover slip: Used to cover the stained slide to protect it from scratching and save it for a long
time.
 Pipettes: Used to transfer solution from one place to another.

A. Graduated pipette: to transfer specific amount of solution.

Use Bi-pump with it.

B. Pasteur pipette: to transfer non specific amount of solution.

 Container: Use it to collect sample from the patient.

It should be sterile.

A. Universal container: 25 ml. Use to collect large amount of sample, like: urine, sputum.

B. Bijou bottle: 5ml. Use to collect small amount of sample, like: CFS, gland fluid.

 Petri dish: Use it to put media inside it for culturing.

It should be sterile.

 Disinfectant jar: Use it for the disposal of some used items, e.g. Slides, cover slips, pipettes.

 Disinfectant: it is a chemical substance that kills or inhibits the growth of micro organisms.

 Incubator:

Incubator is a warm cabinet that you can set its temperature to a proper temperature for bacteria
growth. About 37º C is a good temperature for most bacteria. This is close to the body temperature.

 Autoclave: Use for sterilization. Its cycle (121ºC, under 15 atm pressure for 15-20 min).

 Autoclave bag: Mad of material that tolerates high temperature.

 What is saline?

It is 0.9% Nacl in H2O = 0.9 g of Nacl dissolved in 100 ml water.

 Its function: Keeps the intact morphology of the cell of the bacteria by regulating the
osmotic pressure.

Disposal of used items:

1. Trash bag: for non infectious material. (Tissues, covering plastic).

2. Disinfectant jar.

3. Autoclave: for contaminated glass wears and flasks, which are reusable items.

4. Autoclave bag: for plastic items (Petri dish, container) which are disposable.

You might also like