Potato varieties for table and processing
Vinod Kumar, Pr. Scientist
                CPRI, Shimla
                           Journey of Potato
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    • Potato originated in high Andean region of South America
    • Till 16th century it was unknown to rest of the world.
    • Potato moved from South America to Spain (1570) and UK (1590).
    • Potato’s global voyage began in the 17th century from Europe.
    • In India & Sri Lanka, potatoes were introduced in early 17th century.
    • Potatoes, became one of the most important world crops in a span of
      300 years
                                Potato adaptation
     In Andes of South America, potato was adapted to short days, tropical
      highlands (2,000 and 4,000 m).
     Its equatorial origin makes potato essentially short-day dependent for
      tuberization
     In Europe, tuber formation was inhibited under long photoperiod (long
      days).
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     Long-day acclimation for tuberization to achieve higher tuber yields in
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      Europe.
     Breeding over more than 150 years led to plants tolerating long day
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      condition.
     The mutations in tuber formation regulator allow potatoes to escape
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      the original short day regulation mechanism suited to the Andes, so
      potatoes can be grown in Europe & other northern latitudes of world
     Mutations in the tuber formation regulator gene which occur in
      different combinations in modern potato cultivars, giving rise to early,
      medium and late varieties, depending on the combination of the gene
      variants present in the tetraploid crop (Kloosterman et al. 2013).
    Kloosterman et al. 2013. Naturally occurring allele diversity allows potato cultivation in northern
                              latitudes. 2013. Nature. 14;495( 7440):246-50 .
                              Potato Breeding
    •   Cultivated potato is auto-tetraploid and highly heterozygous.
    •   Heterosis is observed on crossing diverse parents.
    •   Breeding of potato involves hybridization between identified parents and
        selection of superior clones from the progeny.
    •   The genetic constitution of the genotype obtained following hybridization
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        is fixed in seedling stage.
    •
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        Due to vegetative propagation the genetic constitution of potato
        genotype with all its intra- and inter-locus interactions responsible for its
        phenotypic expression are maintained in the clonal generations.
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    •   Hence, a clone if perceived desirable can be multiplied for commercial
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        cultivation even though initially it may be present as a single plant.
    •   More than 50 traits should be combined in a modern potato variety
        (Ross, 1986).
    •   An ideal potato variety affects not only yield and quality but also
        production cost, environmental issues, post harvest and yield of future
        crops (Struik and Wiersema, 1999, Collard and Mackill, 2008).
    •   It can take 10 to 15 years to release a variety.
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C   Potato varieties developed in the world
    Potato breeding has resulted in the release of over 4000
    cultivars in more than 100 countries.
               Need of potato Breeding in India
    Parameters               India                       Europe/America
    Growing season           Winter                      Summer
    Temperature during       High                        Low
    planting &               Planting: 25-32oC           Planting: (15-25oC)
    harvesting               Harvesting: 10-20oC         Harvesting: Less than
                                                         20oC
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    Day & night temp.        Day : 25-32 oC              Day : 20-25 oC
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    during crop season       Night : 3-15 oC             Night : 5-20 oC
    Crop duration (d)        Short (60-100)              Long (150-180)
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    Photoperiod              10 hrs/day                  14 hrs/day
    Frosting                 Common                      Absent
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    Post harvest             Difficult (high temps.)     Easy (low temps.)
    handling
    Result                   Low yields, less dry        High yields, high dry
                             matter, and more            matter & low reducing
                             reducing sugars             sugars
    •   European varieties were long-day adapted
    •   Progressive accumulation of degenerative viral diseases
    •   Physiological limitations on storage & utilization in hot/humid summers
    •   Varieties: Craig’s Defiance, Great Scot, Up-to-Date and Magnum Bonum
                Potato breeding in India
     1935:Potato breeding programme at Potato Breeding Station,
      Shimla.
     1949: CPRI at Patna (Bihar)-Regular breeding programme .
     1956: CPRI at Shimla (HP) to facilitate hybridization & seed
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      health .
     1963: Seed Plot Technique.
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     Potato breeding system developed.
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     This approach yielded for potato improvement and potato seed
      production.
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     CPRI varieties prefixed with ‘KUFRI’ as a memento to place of
      crossing.
                                                       Kufri Bahar
          Germplasm collection and evaluation
     Collection
     Field/Glass house maintenance
     Evaluation
    Selection for
     Vigorous plant type
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     Short duration
     Desirable tuber attributes
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     Early bulking
     High tuber yield, tuber number,
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       average tuber weight
     High dry matter
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     Good keeping quality
     Processing attributes
     Late blight resistance
     Water stress
     Heat stress
     Nutrient use efficiency
     Nutrition quality
    National Potato Germplasm Repository
            Total: ~ 4375 (40 countries)
         Indian varieties and    305
               parental lines
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                                                           2218
                  Tuberosum
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                                                           (30C, 241CC)
         Exotic
                   Andigena                   770 (5C, 77 CC)
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                   Wild spp.                       1082 (5C,118 spp.)
       Nearly 14,000 accessions in CIP gene bank
       Nearly 6000 accessions in US potato gene bank
       Nearly 1500 accessions in Commonwealth potato collection
      Sources of resistance to various diseases in potato
            Diseases                                         Sources
    Viruses -          PVX      S. acaule, S. berthaultii, S. tuberosum subsp. andigena
                       PVY      S. phureja, S. demissum, S. stoloniferum
                     PLRV       S. acaule, S. demissum, S. tuberosum subsp. andigena
    Late blight      Vertical   S. demissum, S. verrucosum, S. stoloniferum
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                  Horizontal    S. berthaultii, S. chacoense, S. microdontum, S. vernei
    Wart                        S. acaule, S. berthaultii.
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    Common scab                 S. chacoense, S. tuberosum ssp. andigena
    Bacterial wilt              S. Chacoense, S. microdontum,
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    Cyst nematodes              S. tuberosum subsp. andigena, S. berthaultii. S. vernei
    Root knot nematode          S. spegazzinii
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    Aphids                      S. berthaultii.
    Frost                       S. acaule, S. ajanhuiri
    Heat tolerance              S. chacoense, S. commersonii
    High protein content        S. phureja
    Most commonly used are: S. berthaultii, S. bulbocastanum, S.
    chacoense, S.microdontum,       S. multidissectum, S. phureja, S.
    sparsipilum, S. stenotomum, S. tarigense, S. vernei, S. demissum
              Breeding Programmes in India
    Breeding for table potatoes (Indo-gangetic plains)
            • North-western plains
            • West-central plains
            • North-eastern plains
    Breeding for late blight (hills)
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            • North-western hills
            • North-eastern hills
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            • Southern hills
            • Darjeeling & Sikkim hills
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    Special breeding programmes
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            • Breeding for processing attributes
            • Breeding for heat tolerance
            • Breeding of drought tolerance
            • Breeding for nutritional quality
            • Breeding for Kharif potatoes
            • Breeding for nutrient efficient varieties
            • Breeding of PCN resistance
                     Potato breeding methodology
     Parental preference                    Selection of parents                   Selection parameters
      i)    S. tuberosum ssp. tuberosum                                            Phenotypic performance,
             (commercial varieties, breeding                                       adaptation,
             lines,  stocks    in   on-going                                       genetic divergence,
             programmes, old land races)
      ii)    S. tuberosum ssp. andigena                                            combining ability, heterosis,
                                                 Hybridization                     progeny tests
     iii)    Primitive cultivated species
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                                                         1) Establishing parents  2.) Pollen fertility estimation
      iv)    Wild tuber bearing species                                              (2% Aceto-carmine)
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     v)      Wild non-tuber bearing species              3) Emasculation       4) Pollen collection 5) Berry, TPS
                                                                                 & pollination          extraction, storage
                               Assessment of segregating population
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    Seedling stage: Good vigour, desirable
    tuber colour, shape, eye depth, no-
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                                                                                                     Weightage
    cracking, irregularity    Evaluation of          clones (F1C1-F1C7)                              Score
    Initial evaluations (F1C1-F1C3) : Un-replicated trials Advanced evaluations (F1C4-F1C7) : Replicated trials
    DUS, DNA
    fingerprinting       Multi-location & on-farm trials under AICRP (P)                            AICRP (P):25
                                                                                                       (18+7)
     Multi-location trials : 2 years                        On-farm trials : 1-2 years
                                    Release & notification of variety
                                    10-15 years of concerted
                         Potato breeding strategy
      Area            Season                   Traits            Potato growing regions
    North     Summer (April-         Long-day adapted,
    Indian    Sep.), Spring          resistant to late blight,
    hills     (Jan/Feb-May/June)
              & Autumn (Aug-
              Nov/ Dec.
    North     Spring (Jan./-April- Short-day adapted,
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    Indian    May) and Autumn      early bulking,
    plains    (Oct.-Jan./ Feb. or  moderately resistant to
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              Nov.-March)          late blight & frost, good
                                   keeping quality
    Plateau   Kharif (July-        Early bulking, ability to
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    region    September), Rabi     tuberize under high
              (Nov.-Feb.)          temperatures, resistant
                                   to bacterial wilt, tuber
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                                   moth and mites, slow
                                   rate of degeneration
    South     Summer (April-       Early bulking, resistant
    Indian    Aug.), Autumn (Sep.- to late blight and cyst
    hills     Dec.), Spring (Jan.- nematodes
              May)
    North     Autumn (Sep.-Dec.) Medium maturing,
    Bengal                         resistant to late blight,
    hills &                        immune to wart, red
    Sikkim                         skinned tubers
           Potato varieties released by CPRI (52)
    Year   Varieties
    1958   Kufri Kisan, Kufri Kuber, Kufri Kumar, Kufri Kundan, Kufri Red, Kufri Safed
    1963   Kufri Neela
    1967   Kufri Sindhuri
    1968   Kufri Alankar, Kufri Chamatkar, Kufri Chandramukhi, Kufri Jeevan, Kufri Jyoti, Kufri Khasigaro, Kufri
           Naveen, Kufri Neelamani, Kufri Sheetman
    1971   Kufri Muthu
    1972   Kufri Lauvkar
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    1973   Kufri Dewa
    1979   Kufri Badshah
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    1980   Kufri Bahar
    1982   Kufri Lalima                                                       2(CS)-21(IxI)-21(IxE)-8 (ExE)=85
    1983   Kufri Sherpa                                                   Adina, Craigs Defiance, Ekishiraju, Katahdin, Up-to-Date
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    1985   Kufri Swarna
    1989   Kufri Megha
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    1996   Kufri Jawahar, Kufri Sutlej, Kufri Ashoka
    1998   Kufri Pukhraj, Kufri Chipsona-1, Kufri Chipsona-2, Kufri Giriraj
    1999   Kufri Anand, Kufri Kanchan
    2005   Kufri Arun, Kufri Pushkar, Kufri Shailja
    2006   Kufri Surya, Kufri Chipsona-3, Kufri Himalini
    2008   Kufri Himsona, Kufri Sadabahar, Kufri Girdhari, Kufri Khyati
    2009   Kufri Frysona
    2010   Kufri Chipsona-4, Kufri Neelma
    2012   Kufri Gaurav, Kufri Garima
    2013   Kufri Lalit, 2015   (Kufri Mohan)
                  Developed 52 improved varieties
                                    Number of varieties
                                            Northern hills
         Early                                                   7      4
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                        9
     (70-80 days)
                                              Plains              6         17         3
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       Medium     33                               Plateau       3 3 1
    (90-100 days)
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                                           Darjeeling hills       3
         Late      10
    (100-110 days)                         Southern hills        3 2
       2(CS)-21(IxI)-21(IxE)-8 (ExE)=85                       Processing-6, Heat tolerant-1
         Breeding for Indo-Gangetic Plains
               (Jalandhar, Modipuram & Patna)
Requirements:
     65-85 days early maturing varieties
     To fit into Rice – Potato – Wheat
     Late blight resistance - not required essentially
Procedure:
 Hybridization at Kufri
         Parents – Tuberosum, Improved Andigena
   • Seedlings/Early generations (F1C1 – F1C3) :- Tuber characters
   • Advance Generations (F1C4 – F1C6)         :- a. Yield trials
                                                  b. Keeping quality
                                                  c. Cooking quality
   • Multilocation Trials:-     (2 years – Replicated
      (under AICRP)              2 years – on farm)
   • Release and Notification
         Breeding for Northern hills
               (CPRI, Shimla; CPRS, Shillong)
Requirements:
  • 100-110 days medium maturing varieties
  • High level of Late blight resistance required
Procedure:
  • Hybridization at Kufri & Shillong
       Parents – Tuberosum, Improved Andigena, wild species
  • Seedlings:                     :- Late blight in lab
  • Early generations (F1C1 – F1C3) :- Tuber characters & LB
  • Advance Generations (F1C4 – F1C6) :- a. Yield trials (Plains & hills)
                                           b. Keeping quality (Plains)
                                           c. Cooking quality
  • Multilocation Trials:-    (2 years – Replicated
     (under AICRP)             2 years – on farm)
  • Release and Notification
         Breeding for Southern hills
                    (CPRS, Ootacamund)
Requirements:
   • 100-110 days medium maturing varieties
   • High level of Late blight resistance required
   • Resistance to cyst nematodes essential
Procedure:
   • Hybridization at Ootacamund & Kufri
        Parents – Tuberosum, Improved Andigena, Vernei clones
   • Seedlings:                     :- Late blight
   • Early generations (F1C1 – F1C3) :- Tuber characters, LB,CN
   • Advance Generations (F1C4 – F1C6) :- a. Yield trials
                                            b. Keeping quality
                                                     (Dormancy)
                                          c. Cooking quality
   • Regional Multilocation Trials:-    (2 years – Replicated
                                          2 years – on farm)
 Regional Release and Notification
         Breeding for Heat Tolerance
Requirements:
       75-90 days early/medium maturing varieties
•      Suitable for early planting in west-central plains
•      Suitable for non-traditional potato growing areas
•      Some level of late blight resistance – desirable
Procedure:
    • Hybridization at Kufri
         Parents – Tuberosum, Improved heat tolerant lines
    • Seedlings:         :-Under normal conditions at Modipuram
    • Early generations :- Early planted crop Modipuram
    • Advance Generations a. Yield trials- Modipuram and plateau
                             b. Keeping quality
                             c. Cooking quality
    • Multilocation Trials:-    (2 years – Replicated
       (under AICRP)             2 years – on farm)
•     Regional Release and Notification
Breeding for Processing
Requirements:
•      90-100 days medium maturing varieties
•      High dry matter, Low reducing sugars
•      Tuber characters - Suitable for chips & French Fry
•      Late blight resistance – required
Procedure:
    • Hybridization at Kufri
         Parents – Tuberosum, Improved Andigena
    • Seedlings:        :-Tuber characters
    • Early generations :- Quality and tuber characters
    • Advance Generations a. Yield and quality trials
                              b. Quality tests at factory
                              c. Keeping quality
     Multilocation Trials:-    (2 years – Replicated
     (Under AICRP)              2 years – On farm)
    Release and Notification
             Breeding for TPS populations
Requirements
•     90-100 days early/medium maturing populations
•      Uniformity in tuber characters and early bulking
•      Late blight resistance required
•      Good seedling survival as transplant
Procedure
• Hybridization at Kufri/Modipuram/Patna
• Seedling evaluation for transplant crop - Shillong
• Seedling evaluation for seedling tuber production -
  Modipuram/Patna/Shillong
  Multilocation testing in North-eastern region
• Multilocation trials under AICRP
• Recommendation/Release
Some Popular Indian Potato Varieties
Kufri Jyoti:
wide adaptability, early bulker,
slow rate of degeneration, immune to wart,
moderately resistant to late blight and early blight
Kufri Bahar:
For North Indian plains,
early bulker, immune to wart,
Resistant to Gemini virus (PALCD)
Kufri Pukhraj:
For North Indian plains and plateau,
Suitable for low input, early bulker, immune to wart,
moderately resistant to late blight and early blight
Kufri Ashoka:
For Indo-gangetic plains,
early maturity
Kufri Chandramukhi:
For North Indian plains and plateau
early maturity
Kufri Badshah:
For North Indian plains and plateau,
medium maturity,
moderately resistant to late blight and
early blight, resistant to PVX
Kufri Sindhuri:
For North Indian plains,
late maturity,
moderately resistant to early blight, tolerant to PLRV
Kufri Kanchan:
For North-Bengal hills and Sikkim,
medium maturity, immune to wart
moderately resistant to late blight,
slow rate of degeneration
Kufri Swarna:
For southern hills, medium maturity,
resistant to cyst nematodes, immune to wart
resistant to late blight and early blight
Kufri Lauvkar:
For plateau, early maturity,
heat tolerant
Kufri Surya:
For plateau, early maturity,
heat tolerant
         Kufri Chipsona-1:
         For North Indian plains, medium maturity
         high dry matter, low reducing sugars, low phenols
         resistant to late blight
         Kufri Chipsona-3:
         For North Indian plains, medium maturity
         high dry matter, low reducing sugars, low phenols
         resistant to late blight, immune to wart
Some latest releases
1.   Kufri Pushkar
2.   Kufri Khyati
3.   Kufri Sadabahar
4.   Kufri Himalini
5.   Kufri Himsona
6.   Kufri Girdhari
7.   Kufri Gaurav
8.   Kufri Garima
9.   Kufri Mohan
                           Recent releases from CPRI
                             Selection          Year of
S. N.   Variety                                           Parentage
                             number             release
36      Kufri Arun           MS/92-2105         2005      Kufri Lalima x MS/82-797
37      Kufri Pushkar        JW 160             2005      QB/A 9-120 x Spatz
38      Kufri Shailja        SM/87-185          2005      Kufri Jyoti x EX/A 680-16
39      Kufri Surya          HT/92-621          2006      Kufri Lauvkar x LT-1
40      Kufri Chipsona-3     MP/97-583          2006      MP/91-86 x Kufri Chipsona-2
41      Kufri Himalini       SM/91-1515         2006      I-1062 x Bulk pollen (CP2132-Tollocan)
42      Kufri Himsona        MP/97-644          2008      MP/92-35 x Kufri Chipsona-2
43      Kufri Sadabahar      MS/93-1344         2008      MS/81-145 x PH/F-1545
44      Kufri Girdhari       SM/93-237          2008      Kufri Megha x Bulk Pollen (10 genotypes)
45      Kufri Khyati         J/93-86            2008      MS/82-639 x Kufri Pukhraj
46      Kufri Frysona        MP/98-71           2009      MP/92-30 x MP/90-94
47      Kufri Neelima        OS/93-D-204        2010      E/79-15 x E/79-42
48      Kufri Chipsona-4     MP/01-916          2010      Atlantic x MP/92-35
49      Kufri Gaurav         JX576              2010      JE 812 x K. Jyoti
50      Kufri Garima         MS/99-1871         2011      PH/F 1045 x MS/82-638
51      Kufri Mohan          MS/5-1543          2015      MS/92-1090 x CP 1704 (Claudia)
        TPS Population       83-P-47 (I) x D-
51                                              2007
                 Potato: Many uses
Starch   Par-fried   Snacks & extruded   Cooked   Dried (Granules,
                         products                    flakes etc.)
    Need for breeding processing varieties
•   All the 29 varieties bred by CPRI till 1998 were mainly for
    table consumption
•   Processing was at a low ebb till 1998
•   With the entry of MNC’s in potato processing sector in
    1998, a need was felt for suitable processing varieties
    having high dry matter and low reducing sugars
•   In the absence of Indian processing varieties, an American
    variety Atlantic was introduced in India, which gave lower
    yields under short day conditions and was susceptible to
    late blight
                                                     Cont…..
    Need for breeding processing varieties
•   A challenge was thrown to the CPRI for developing
    indigenous processing variety
•   After 8 years of dedicated efforts, India’s first processing
    varieties namely, Kufri Chipsona-1 and Kufri Chipsona-2,
    were released
•   Another improved processing variety, Kufri Chipsona-3,
    producing higher proportion of processing grade tubers
    with no tuber defects was developed in 2005
•   An exclusive chipping variety, Kufri Himsona, was
    released for hilly regions of India in 2007
Quality requirements of potatoes for different
         forms of processed products
Characteristics                             Products
                              Dehydrated French fries       Chips       Canned
Tuber shape                   Round to       Oblong       Round to      Round to oval
                                oval                        oval
Tuber size, mm                    30           >75          45-80          20-35
Eyes                           Shallow       Shallow       Shallow        Shallow
Specific gravity                1.080         1.080        > 1.080        < 1.070
Dry matter, %                    >20           >20           >20            <18
Reducing sugar (% fresh wt)      0.15          0.15         < 0.1            0.5
After cooking discoloration     Slight        Slight           -             Nil
Texture                       Fairly firm   Fairly firm   Fairly firm      Waxy
                               to mealy                    to mealy
Desirable biochemical attributes for
   breeding processing varieties
Parents selected for breeding varieties should have following traits
   The dry matter should be >20% and reducing
    sugar content should be <0.1% on Fresh wt
    basis
   No enzymatic browning
   The glycoalkaloid content should be <20 mg/100
    g Fresh wt
Salient features of Indian processing varieties
 Kufri Chipsona-1
                    •Selection from the progeny of the cross CP
                    2416 x MS/78-79 made in 1990
                    •The plant has medium compact canopy with
                    white flowers and the tubers are white
                    cream, ovoid with shallow eyes and white
                    flesh
                    • The variety is well adapted to north-Indian
                    plains and has a maturity period of 90-110
                    days
                    • It has resistance to late blight and gives an
                    average yield of 300-350 q/ha and possess
                    very good storability
Salient features of Indian processing varieties
  Kufri Chipsona-2
                     •Selection from the progeny of the cross CP
                     2346 (F-6 from Peru) x QB/B 92-4 made in
                     1991
                     •The plant has medium compact canopy with
                     white flowers and the tubers are white cream,
                     round with shallow eyes and creamy flesh
                     • The variety is well adapted to north-Indian
                     plains and has a maturity period of 100-110
                     days. It is resistant to late blight and gives an
                     average yield of 300-325 q/ha
Salient features of Indian processing varieties
 Kufri Chipsona-3
                    •Selection from the progeny of the cross Kufri
                    Chipsona-2 x MP/91-86 made in 1996
                    • The plant has medium compact canopy with
                    white flowers and the tubers are yellow, ovoid
                    with medium-deep eyes and creamy flesh
                    • The variety is well adapted to north-Indian
                    plains and has a maturity period of 90-110
                    days. It is resistant to late blight and gives an
                    average yield of 350-400 q/ha
Salient features of Indian processing varieties
   Kufri Himsona
                   •Selection from the progeny of the cross
                   MP/92-35 x Kufri Chipsona-2
                   • The plant has medium canopy with purple
                   flowers and the tubers are white to creamy,
                   round-oval with shallow eyes and cream pale
                   yellow flesh
                   • The variety is adapted to hilly regions of
                   the country and has a maturity period of 120-
                   140 days
                   •It has field resistance to late blight and
                   gives an average yield of 250 q/ha.
Features of French fry variety Kufri Frysona
                (MP/98-71)
                        Total yield: 38.3 t/ha
                        French fry grade yield: 19.8 t/ha
                        Dry matter: 23.0%
                        Excellent fry colour
                        Very good storability at 10-12 oC
                        with CIPC
Features of promising early maturing hybrid
       MP/01-916 (Kufri Chipsona-4)
               • Higher total and processing grade
                 yield
               •Excellent chip colour
               •High specific gravity (1.083) and dry matter
               (24.3%)
               •Low level of reducing sugars (0.03%)
               •Moderately resistant to late blight in
               comparison to popular variety Kufri Jyoti and
               exotic Atlantic
Biochemical traits and suitability of Indian processing
                      varieties
 Varieties           Dry     Reducing            Suitability for
                    matter      sugars             Products
                     (%)     (mg/100 g
                                 FW)
 Kufri Chipsona-1   21-24     45-100     Chips, French fries, flakes,
                                            flour, dehydrated products
 Kufri Chipsona-2   21-25     45-95      Chips, flakes, flour,
                                         dehydrated products
 Kufri Chipsona-3   21-24     50-70      Chips, flakes, flour,
                                         dehydrated products
 Kufri Himsona      22-26     45-65      Chips, flakes, flour,
                                         dehydrated products
 Kufri Frysona      21-23     80-100     French Fries
 Impact of Indian processing varieties
• The long pending demand of the industries was met as
  Indian processing varieties contained >21% dry matter
  and low reducing sugars (<0.1% on fresh wt)
• All these varieties are suitable for chips and
  dehydrated products
• Kufri Chipsona-1 due to its oval to oblong tuber shape
  is utilized both by the chipping and French fry
  industries
• These varieties have brought revolution in the Indian
  processing scenario within a span of 10 years
             Future thrust
Development of French fry varieties
Development of cold chipping variety
Development of early maturing chipping
 variety
Thanks