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MCQ On Electrophoresis

This document contains 10 multiple choice questions about electrophoresis techniques. It asks about concepts like how an electric potential gradient is created across electrodes, factors that determine the velocity of charged particles in an electric field, common electrophoresis methods used to separate DNA and proteins, the purpose of reagents like SDS and β-mercaphoethanol in SDS-PAGE, and definitions of terms like electrophoretic mobility.

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9K views2 pages

MCQ On Electrophoresis

This document contains 10 multiple choice questions about electrophoresis techniques. It asks about concepts like how an electric potential gradient is created across electrodes, factors that determine the velocity of charged particles in an electric field, common electrophoresis methods used to separate DNA and proteins, the purpose of reagents like SDS and β-mercaphoethanol in SDS-PAGE, and definitions of terms like electrophoretic mobility.

Uploaded by

Hunzala
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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1). When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a
potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as:

a. E = V/d
b. E = (1/V) x q
c. E = (Vd)/q
d. E=V+d

2). The velocity (‘v’) of a charged particles in an electric field in a medium can be
mathematically expressed as v = Eq/f, where ‘Eq’ and ‘f’ are ________.

a. Eq: Energy; f: Frictional force


b. Eq: Electrical force; f: Gravitational force
c. Eq: Electrical force; f: Frictional co-efficient
d. Eq: Equilibrium constant; f: co-efficient of gravity

3). For the separation of DNA by electrophoresis, which of the following method is
commonly used?

a. Agarose – vertical
b. Agarose – horizontal
c. PAGE – vertical
d. PAGE – horizontal

4). Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________.

a. An anionic detergent
b. A cationic detergent
c. A non-ionic detergent
d. An anion exchanger
e. A cation exchanger

5). Function of β-mercaptoethanol in SDS-PAGE is__________.

a. To give negative charges to amino acids in the proteins


b. For the oxidation of disulfide bonds in the proteins
c. For the reduction of disulfide bonds in the proteins
d. For breaking hydrogen bonds in the proteins

6). The ratio of velocity (‘v’) of biomolecule in a medium under constant electric field
(‘E’) is called ‘Electrophoretic mobility’ denoted as ‘µ’. ‘µ’ is mathematically expressed
as:

a. µ = E/v
b. µ = v/E
c. µ = 1/(Ev)
d. µ = VE
7). In electrophoresis, the electrophoretic mobility (‘µ’) is determines the characteristics
of migration of different biomolecules. Which of the following is not having any
influence in ‘µ’?

a. Stereochemistry of molecule
b. Size of molecule
c. Shape of molecule
d. Molecular weight
e. Net charge of molecule

8). Electrophoresis is not used for the separation of ________.

a. Nucleic acids
b. Proteins
c. Amino acids
d. Lipids

9). In SDS-PAGE of protein separation, one SDS molecule will binds to __________.

a. Every amino acid


b. Every two amino acids
c. Every three amino acids
d. Every Four amino acids

10). In SDS-PAGE, migration of protein is effected by ____________.

a. Charge of protein
b. Size of protein
c. Net charge of protein
d. All of these

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