MCQs for instrumental methods of

analysis - BP701TP

Ultra Violet Spectroscopy MCQs

1. UV SPECTROSCOPY working on which phenomenon ?
A. Absorption
B. Elution
C. A and B
D. None of this
2. Ultra Violet spectroscopy wavelength is ?
A. 200 nm to 300nm
B. 200nm to 400nm
C. 400nm to 800 nm
D. All of the above
3. Visible spectroscopy wavelength range is ?
A. 200 nm to 300nm
B. 200nm to 400nm
C. 400nm to 800 nm
D. All of the above
4. n⇒σ∗ transition is seen in which compound ?
A. Alcohol
B. Aromatic compound
C. Carbonyl compound
D. None of this
5. In uv SPECTROSCOPY water show peak on which λmax ?
A. 191 nm
B. 300 nm
C. 400 nm
D. 500 nm
6. Carbonyl compound shows Which transition
A. π⇒π∗
B.σ⇒σ∗
C.n⇒σ∗
D.n⇒π∗
7. Aromatic compound shows which transition
A. π⇒π∗
B.σ⇒σ∗
C.n⇒σ∗
D.n⇒π∗
8. During n⇒π∗ show wave length is ?
A. More than 200 nm
B. Less than 200 nm
C. A and B
D. All of the above
9. Which compound shows π⇒π∗ ?
A. Double and triplet bond
B. Aromatic compound
C. A and B
D. None of this
10. Hydrocarbon are seen transition is ?
A. π⇒π∗
B.σ⇒σ∗
C.n⇒σ∗
D.n⇒π∗

Answer key
1. A 2. B 3. C 4. A 5.A 6. D 7. A 8. A 9. C 10. B

1. Which statement is false about Chromophore ?

A. It is co-valent unsaturated compound.
B. It is contain π electron.
C. They required high energy for electron transition.
D. They required low energy for electron transition.
2. Which statement is true about Auxochrome ?
A. It is only saturated compound.
B. It is only unsaturated compound.
C. It is Decreased intensity of radiation.
D. They are saturated and unsaturated compound.
3. Which group act as a Chromophore?
A. -N=N-
B. -OH
C. -OR
D.- NH2
4. DNA denaturation is example of which type of shift ?
A. Bathochromic
B. Blue shift
C. Hyperchromic shift
D. Red shift
5. λmax increase with absorption of radiation in which shift ?
A. Red shift
B. Blue shift
C. A and B
D. None of this
6. Due to deformation of compound due to attached some other functional group it is example of
A. Red shift
B. Blue shift
C. Hyperchromic shift
D. Hypochromic shift
7. Auxochrome have which type of electron ?
A. Non bonded
B. π electron
C.σ electron
D. None of this
Answer key
1. C
2. D
3. A
4. C
5. A
6. C
7. A

Q1.
Sentence A: if temp. Is increases UV radiation absorption is increase.
Sentence B: If temp. Is increases it is give better result in uv.
A. Sentence A and B is false.
B. Sentence A and B is true.
C. A is true and B is false.
D. A is false amd B is true.
2. Which sentence is false about UV SPECTROSCOPY ?
A. Benzene is not used as solvent.
B. Low temperature is give better result in uv SPECTROSCOPY.
C. Chloroform is used as solvent in UV.
D. UV ray wavelength is 200- 400 nm.
3. Which sentence is true according to UV-VISIBLE SPECTROSCOPY ?
A.The principle is based on the measurements of spectrum of a sample containing atom/molecules.
B.UV-Visible spectroscopy measure the response of a sample to ultra Violet and visible range of EMR.
C.Spectrometer is an instrument design to measure the spectrum of a compound.
D. All of the above
4. Which sentence is false about inorganic compound ?
A. Ag is increase absorption of UV radiation.
B. AU is increase absorption of UV radiation.
C. Inorganic compound are decreased uv radiation absorption.
D.Inorganic compound are increased uv radiation absorption.
5.when absorption intensity of compound is decreased it is called
A. Red shift
B. Blue shift
C. Hypochromic shift
D. Hyperchromic shift
6. Which compound is increased uv absorption ?
A. Auxochrome
B. Chromophore
C. Inorganic compound
D. All of the above
7. If solvent is polar ans electron transition is n->π∗ which type of shift are seen in graph ?
A. Hypso chromich shift
B. Red shift
C. Blue shift
D. A and C
8.If solvent is non polar ans electron transition is n->π ∗which type of shift are seen in graph ?
A. Hypso chromich shift
B. Red shift
C. Blue shift
D. A and C
9.If solvent is non polar ans electron transition is π⇒π∗which type of shift are seen in graph ?
A. Hypso chromich shift
B. Red shift
C. Blue shift
D. A and C
10. If solvent is polar electron transition is π⇒π∗which type of shift are seen in graph ?
A. Hypso chromich shift
B. Red shift
C. Blue shift
D. A and C

Answer key
1.C
2.C
3.D
4.C
5.C
6.D
7.D
8.A
9.B
10.D

1. Tungsten lamp filament has required how much temperature ?

A. 2000k
B. 3000k
C. 4000k
D. 5000k
2.How much range wavelength is transmit by silicate glass ?
A. 100 nm to 200 nm
B. 200nm to 300 nm
C. 300 nm to 350 nm
D. 10nm to 40 nm
3. what is role of slit in uv-visible spectroscopy ?
A. Monochromatic radiation to polychromatic radiation.
B. Polychromatic radiation to monochromatic radiation.
C. A and B
D. None of this
4. Which radiation source has electrode in its construction ?
A. Tungsten lamp
B. Hydrogen discharge lamp
C. Xenon Discharge Lamp
D. Mercury lamp
5. Which device is used to isolate the radiation of the desired wavelength from wavelength of the
continuous spectra ?
A. Monochromator
B. Radiation source
C. Recorder
D. None of this
6. Diffraction grating is consists of a
A. Glass
B. Quartz
C. Alkyl halide
D. All of the above
7. The work of Entrance slit is?
A. To get narrow source
B. To render light parallel
C. To disperse radiation
D. To fall on sample cell
8. Collimator is used for?
A. To reform the image of the entrance slit.
B. To get narrow source
C. To fall on sample cell
D. To disperse radiation
9. Exit slit used for
A. To reform the image of the entrance slit.
B. To get narrow source
C. To fall on sample cell
D. To disperse radiation
10. Which type of vapour is stored in Mercury lamp ?
A. Mercury vapour
B. Hydrogen vapour
C. Xe
D. Ozone
Answer key
1. B
2. C
3. B
4. B
5. A
6. D
7. A
8. A
9. C
10. A

1. Which material are used to made sample cell ?

A. Silver
B. Silicon
C. Quartz
D. None of this
2. The pathlength of the sample cell is
A. 100cm
B. 1 cm
C. 1009 cm
D. 10 cm
3. Which device is used to convert light energy into electrical signal ?
A. Amplifier
B. Detector
C. Sample cell
D. Slit
4. How many potential difference between two electrode in PMT ?
A. 75 – 100 volt
B. 109 volt
C. 100-105 volt
D. 50- 75 volt
5. Which material are used to coated cathode ?
A. Gold
B. Silver
C. A and B
D. None of this
6. In photo voltaic cell which material is used as layer between cathode and anode ?
A. Selenium
B. Aluminum
C. Au
D. Ag
Answer key
1. C
2. D
3. B
4. A
5. C
6. A

Instrumentation of Fluorimetry MCQs

1. When molecules are excited in visible region which lamp is used ?
A. Hydrogen deturium lamp
B. Xenon lamp
C. Tangustan lamp
C. Mercury lamp
Ans. B
2. Which filter are absorbed UV radiation and transmit visible radiation ?
A. Primary filter
B. Secondary filter
C. A and B
D. None of this
Ans. B
3. Which filter are absorbed visible radiation and transmit UV radiation ?
A. Primary filter
B. Secondary filter
C. A and B
D. All of the above
Ans. A
4. At low pressure Mercury lamp liner radiation wavelength is ?
A. 254 nm
B. 700 nm
C. 800 nm
D. 600 nm
Ans. A
5. Sample cell shape is
A. Cylindrical
B. Quadrangular
C. A and B
D. None of this
Ans. C
6. How many mm path length is sample cell ?
A. 10 mm
B. 20 mm
C. 30 mm
D. 40 mm
Ans.A
7. During first rotation of chopper radiation beam is transmit through ?
A. Sample cell
B. Reference cell
C. A and B
D. None of this
Ans. B
8. During second rotation of chopper radiation beam is transmit through ?
A. Sample cell
B. Reference cell
C. A and B
D. None of this
Ans. A
9. Which detector are used in Fluorimetry?.
A. Photo voltaic cell
B. PMT
C. Photo tube
D. All of the above
Ans. D
10. Which are the varient of Fluorimetry instrument ?
A. Single beam
B. Double beam
C. Spectroflourimeter
D. All of the above
Ans. D

IR spectroscopy MCQs
1. What is the frequency of the mild IR ?
A. 0.7 – 2.5μ
B. 2.5 -50μ
C. 50-200μ
D. All of the above
Ans. B
2. Which is not use of IR spectroscopy ?
A. Functional group
B. Structure identify
C. A and B both
D. Metal detecting
Ans.D
3. Which is principal of the IR spectroscopy ?
A. Absorption
B. Emissions
C. Adsorption
D. Nome of this
Ans. A
4. which is important region in IR spectra ?
A. Finger print region
B. Functional group region
C. A and B both
D. None of this
Ans .C
5. Which movement is required for the IR spectroscopy ?
A. Dipole movement
B. Spin movement
C. Round movement
D. All of the above
Ans. A
6. Which type of vibration bond angle is decrease ?
A symmetrical
B. Asymmetrical
C. Rocking
D. Scissoring
Ans. D
7 . In which vibration bond length is increase or decrease ?
A. Rocking
B. wagging
C. Twisting
D. Symmetrical
Ans. D
8. In which vibration one bond is one plane and other bond is another plane?
A. Rocking
B. Wagging
C. Scissoring
D. Twisting
Ans .D
9. In which bending type of vibration bond angle is constant ?
A. Scissoring
B. Twisting
C. Rocking
D. All of the above
Ans. C
10. Which IR frequency are absorbed by compound ?
A. Applied frequency of the IR is equal to the natural frequency of the compound
B.Applied frequency of the IR is low to the natural frequency of the compound
C.Applied frequency of the IR is greater to the natural frequency of the compound
D. None of this.
Ans. A

1. Nujol mean
A. Polymer
B. Crude oil
C. Mineral oil
D. Volatile oil
Ans.C
2. Which of the following is used in calibration of IR instrument ?
A. TMS
B. Glass
C. Mental halide
D. Poly styrene
Ans. D
3. Which material are used to prepare rod of globar source ?
A. Silicon dioxide
B. Silicon carbide
C. Silicon oxide
D. All of the above
Ans.B
4.Which temperature is required for nernst glower to produce IR radiation ?
A. 1000-1800 c
B. 1330- 1200 c
C. 800- 900 c
D. 100-300 c
Ans A
5. Which compound are used as diluent in IR sampling ?
A. alkali halide
B. keton
C. Aldehyde
D. Acetone
Ans. A
6. Monochromator filter are made up of _?
A. Hydrogen peroxide
B. Silicon
C. Lithium flouride
D. All of the above
Ans . C
7. Which detector are detected IR radiation by potential different ?
A. Thermocouple
B. Bolometers
C. Thermistor
D. None of this
Ans. A
8. Nernst glower consists of
A. Ziroconium
B. Yetrium
C. Chromium
D. All of the above
Ans .D
9. Incandescent lamp how much temperature is Required to produce IR radiation ?
A. 1100 c
B. 800 c
C 300 c
D. 1400 c
Ans. A
10. Globar source required IR radiation temperature?
A. 1000 – 1800 c
B. 1000 – 1100 c
C. 700-800 c
D. 6000c
Ans. A

Flame Photometry MCQs

1. Which of the following is the principle of Flame emission photometers?

a) Radiation is absorbed by non-excited atoms in vapour state and are excited to higher states
b) Medium absorbs radiation and transmitted radiation is measured
c) Colour and wavelength of the flame is measured
d) Only wavelength of the flame is measured
2. Which of the following is not an advantage of Laminar flow burner used in Flame photometry?
a) Noiseless
b) Stable flame for analysis
c) Efficient atomization of sample
d) Sample containing two or more solvents can be burned efficiently
3.Which of the following is not a detector used in Flame emission photometers?
a) Photronic cell
b) Photovoltaic cell
c) Photoemissive tube
d) Chromatogram
4. If Propane and air are used in burner how many °C temp. is produced ?
a)1900°C
b)2200°C
c)2300°C
d)2100°C
5. If Hydrogen and air are used in burner how many °C temp. is produced ?
a)1900°C
b)2200°C
c)2300°C
d)2100°C
6. Which is not application of flame photometry ?
a)To estimate sodium, magnesium, calcium
b) Assay of metformin.
c)Used to determine magnesium and calcium in cement.
d) To detected metalic ions in sample.
7. BOLTZMANN Equations is
a)N* / N =R Ae–∆E/kT
b)N* / N = e–∆E/kT
c)N* / N = Ae–∆E/kT
d)N* / N = Ae–∆E/kc
8. Choose correct sequence of flame photometry ?
a) Sample residue → excited state atoms → Return in ground state → Emission of radiation
b)Sample residue → ground state → excited state → Emission of radiation
c) Emission of radiation → excited state →ground state → Sample residue
d) Sample residue → ground state → excited state → Emission of radiation
9. Which equations are used to determine wavelength of radiation ?
a)λ = hc/ E2-E1
b)N* / N = e–∆E/kT
c) λ = h/E2-E1
d)N* / N = Ae–∆E/kT
10. Which is application of flame photometry ?
a) To determine functional group
b) To study of chemical structure
c) To assay of drug
d) To estimate metalic ions like sodium, potassium, etc.
ANSWER KEY :-
1. c
2.d
3.d
4.d
5.a
6.b
7.c
8.a
9.a
10.d

Atomic Absorption spectroscopy MCQs

1 .Choose the correct sequence of process during Atomization in atomic absorption spectroscopy?
(GPAT 2019)
(a) Desolvation → Nebulization→ Dissociation →Volatilization → Ionization ion
(b) Nebulization → Desolvation → Volatilization →Dissociation → Ionization ion
(c) Desolvation → Nebulization → Volatilization → Dissociation →Ionization ion
(d) Nebulization →Volatilization → Desolvation →Dissociation →Ionization
2. In Atomic Absorption Spectroscopy, which of the following is the generally used radiation source?
(a) Tungsten lamp
(b) Xenon mercury arc lamp
(c) Hydrogen or deuterium discharge lamp
(d) Hollow cathode lamp
3. Which of the following is not a component of the emission system in Flame photometer?
(a) Burner
(b) Atomiser
(c) Fuel gases and their regulation
(d) Chopper
4. Which fuel is produced 3300-3400 temperature ?
(a) Acetylene+Air
(b)Acetylene+Oxygen
(c)Acetylene+Nitrous oxide
(d)Hydrogen+Air
5. Which fuel is produced 2400-2700 temperature ?
(a) Acetylene+Air
(b)Acetylene+Oxygen
(c)Acetylene+Nitrous oxide
(d)Hydrogen+Air
6. What is length and diameter of Hollow Graphic cylinder ?
(a) 50 mm and 9 mm
(b) 68 mm and 10 mm
(c) 100 mm and 80 mm
(d) 25 mm and 10 mm
7. Which instrument is used to convert sample in mist or aersol ?
(a) Atomizer
(b) Hollow cathode lamps
(c) Nebulizer
(d) Detector
ANSWER KEY
1.c
2.d
3.d
4.b
5.a
6.a
7.c

Nephelometry & Turbidimetry MCQs

1. What is principal of Nephelometry ?

A. Light scattered
B. Light transmitted
C.A and B
D. None of the above
2. What is principal of Turbidimetry?
A. Light scattered
B. Light transmitted
C.A and B
D. None of the above
3.The intensity of the scattered light is usually measure at which angle?
A. 90°
B. 44°
C. 60°
D. 70°
4. The intensity of the transmitted light is usually measure at which angle?
A. 80°
B. 180°
C. 90°
D. 100°
5. Which technique is used to analyze colloidal system?
A. Nephelometry
B. Turbidimetry
C. A and B
D. None of the above
6. Which sentence is false about Nephelometry ?
A. Nephelometry is concerned with the measure of the intensity of the transmitted light as a function of
concentration of the suspended particle in a suspension.
B.Intensity of scattered light is directly proportional to the concentration of the suspended particle.
C.Hence greater concentration of particle more the intensity of light
D.The intensity of the scattered light is usually measure at 90° to the incident light.
7. Which sentence is false about Turbidimetry ?
A.Turbidimetry is concerned with the measure of the intensity of the transmitted light as a function of
concentration of the suspended particle in a suspension.
B.The intensity of transmitted light is measured in a line i.e 180° to the incident light.
C.Hence concentration is more transmission is less.
D.Hence greater concentration of particle more the intensity of light.
Answer key :-
1. A
2. B
3. A
4. B
5. C
6. A
7. D

1. Nephelometry is similar to
A. fluorimetry
B. Colorimetery
C. UV-VISIBLE
D. NMR
2. Turbidimetry is similar to
A. fluorimetry
B. Colorimetery
C. UV-VISIBLE
D. NMR
3. Which filter are used as secondary filter in Nephelometry?
A. Visible filter
B. Absorption filter
C. A and B
D. None of the above
4. Which is filter are used in Turbidimetry ?
A. Blue filter
B. Visible filter
C. Absorption filter
D. All of the above
5. Which sentence is true about Nephelometry ?
A. It is less sensitive.
B. Wavelength is more important.
C. Intensity of the light and concentration graph is linear.
D. It is not used in analysis of the collodial systems.
6. Which sentence is True about Turbidimetry ?
A. It is more sensitive.
B. Wavelength is not important.
C.Intensity of the light and concentration graph is linear.
D. It is similar to colorimetery .
7. Which sentence is false about Nephelometry ?
A. It is more sensitive (>100mg/litre)
B. Wavelength is not important.
C. Intensity of the light and concentration graph is linear.
D. It is not used in analysis of the collodial systems.
8.Which sentence is false about Turbidimetry ?
A. It is more sensitive.
B. Wavelength is important according to the equation.
C. intensity of the light and concentration realtion ship is according to the equation.
D. It is similar to colorimetery .

Answer key :-
1. A
2. B
3. A
4. A
5. C
6. D
7. C
8. A