Semester – I

Course Code Course Type Course Title Credits Lectures/Week

SBT101 Core Subject Basic Chemistry-I 2 3

SBT102 Core Subject Basic Chemistry-II 2 3

SBT103 Core Subject Basic Life Sciences-I : Biodiversity and Cell Biology 2 3

SBT104 Core Subject Basic Life Sciences-II : Microbial Techniques 2 3

SBT105 Core Subject Basic Biotechnology-I : Introduction to Biotechnology 2 3

SBT106 Core Subject Basic Biotechnology-II : Molecular Biology 2 3

Ability
SBT107 Societal Awareness 2 3
Enhancement
Course 1 (FC I)
SBTP101,
SBTP102, Core Subject Practicals of SBT101, SBT102, SBT103, SBT104, 6 18
SBTP103 Practicals SBT105 and SBT106

Semester – II
Course Code Course Type Course Title Credits Lectures/Week

SBT201 Core Subject Chemistry-I : Bioorganic Chemistry 2 3

SBT202 Core Subject Chemistry-II : Physical Chemistry 2 3

SBT203 Core Subject Life Sciences-I : Physiology and Ecology 2 3

SBT204 Core Subject Life Sciences-II : Genetics 2 3

SBT205 Core Subject Biotechnology-I : Tissue Culture & 2 3

Scientific Writing and Communication Skills
SBT206 Core Subject Biotechnology-II : Enzymology, Immunology 2 3
and Biostatistics
Ability
SBT207 Globalization, Ecology and Sustainable Development 2 3
Enhancement
Course 2 (FC II)
SBTP201,
SBTP202, Core Subject Practicals of SBT201, SBT202, SBT203, SBT204, 6 18
SBTP203 Practicals SBT205 and SBT206
COURSE OUTCOME FOR BIOTECHNOLOGY
F.Y.BSc Sem I

SBT 101
Basic Chemistry I
Course Objective : To acquaint the students with basic concepts of Chemisty like
Classification and Nomenclature of Chemical compounds
Learning Outcome : To impart hands-on skills in preparation of Buffers and Solutions

SBT 102
Basic Chemistry II
Course Objective : To acquaint students with Concepts of Stereochemistry
Learning Outcome : To impart knowledge of Titrimetric and Volumetric Estimations
and handling of basic Analytical Techniques like Chromatography and Colorimetry

SBT 103
Life Science I
Biodiversity and Cell Biology
Course Objectives: To acquaint students with concept of Biodiversity and Cell Biology
Learning Outcome : To impart skill in handling and culture of Microorganisms

SBT 104
Life Science II
Microbial Techniques
Course Objectives: To aquaint students with basic techniques in Staining and
Sterilization
Learning Outcome: To impart the knowledge of growth of microorganisms

SBT 105
Biotech I
Introduction to Biotechnology
Corse Objectives : To auaint students with various fields of Biotechnology and their
applications
Learning Outcome : To impart the knowledge of Food Technology and Fermentation
Techniques

SBT 106
Biotech II
Molecular Biology
Corse Objectives : To acquaint students with DNA Replication, Repair and Genetic
Engineering
Learning Outcome: Impart the knowledge of molecular Biology Techniques

SBT 107
Ability Enhancement (FC I)
Social Awareness
Course Objective : To acquaint the students with concepts of Societal Awarness
Learning Outcome : To impart knowledge of Society and make students aware about
the Problems in Society
F.Y.BSc Sem II
SBT 201
Basic Chemistry I
Bioorganic chemistry
Course Objectives : To acquaint students with Bioorganic Molecules
Learning Outcome : To impart the knowledge of Classification, Strucure and
Characterization of Biomolecules

SBT 202
Basic Chemistry II
Physical Chemistry
Course Objectives : To acquaint students with concepts in Thermodynamics, Kinetics
and Redox Reactions
Learning Outcome : To impart skills in Kinetics and Chemical Reactions

SBT 203
Life Science I
Physiology and ecology
Course Objectives: To acquaint students with Physiological Processes in Plants and
Animals
Learning Objectives : To impart the knowledge of Physiology and Ecology

SBT 204
Life Science II
Genetics
Course Objectives : To acquaint students with concepts in Genetics
Learning Objectives: To impart skills in Techniques in Genetic Analysis and
Population Genetics

SBT 205
Biotech I
Tissue Culture & Scientific
Writing and Communication
Skills
Course Objectives : To acquaint students with Techniques of Plant and Animal Tissue
Culture
Learning Outcome :To impart the skills of PTC, ATC and Science Communication

SBT 206
Biotech II
Enzymology, Immunology and
Biostatistics
Course Objectives : To acquaint students with concepts in Enzymology, Immunology
and Biostatistics
Learning Outcome: To impart the skills in Enzyme Kinetics, Immunological
Techniques and Biostatistics

SBT 207
Ability Enhancement (FC II)
Globalization, Ecology and
Sustainable Development
Course Objective : To acquaint the students with concepts of Globalization, Ecology
and Environment
Learning Outcome : To impart knowledge of Globalization make students aware about
the Problems in Society.
SEMESTER – I
THEORY
 SEMESTER I

Basic Chemistry-I

COURSE CODE TITLE CREDITS

SBT 101 Basic Chemistry I 2

Nomenclature and Classification of 15 Lectures
Unit I Inorganic Compounds:
Nomenclature and Oxides, Salts, Acids, Bases, Ionic,
Classification Molecular and Coordination Compounds

Nomenclature and Classification of

Organic Compounds: Alkanes, Alkenes,
Alkynes, Cyclic Hydrocarbons, Aromatic
Compounds, Alcohols and Ethers,
Aldehydes and Ketones, Carboxylic Acids
and its derivatives, Amines, Amides, Alkyl
Halides and Heterocyclic Compounds
Chemical Bonds: 15 Lectures
Unit II Ionic Bond: Nature of Ionic Bond,
Chemical Bonds Structure of NaCl, KCl and CsCl, factors
influencing the formation of Ionic Bond.

Covalent Bond: Nature of Covalent Bond,

Structure of CH4, NH3, H2O, Shapes of
BeCl2, BF3

Coordinate Bond: Nature of Coordinate

Bond

Non Covalent Bonds: Van Der Waal‘s

forces: dipole - dipole, dipole –
induced dipole.

Hydrogen Bond: Theory of Hydrogen

Bonding and Types of Hydrogen Bonding
(with examples of RCOOH, ROH,
Salicylaldehyde, Amides and Polyamides).
Chemistry of Water: 15 lectures
Unit III Properties of Water, Interaction of Water
Water and Buffers with Solutes (Polar, Non-Polar, Charged),
Non-Polar Compounds in Water – Change
in its Structure and the Hydrophobic Effect,
Role of Water in Biomolecular Structure
and Function and Water as a Medium for
Life
 Solutions: Normality, Molarity, Molality,
Mole fraction, Mole concept, Solubility,
Weight ratio, Volume ratio, Weight to
Volume ratio, ppb, ppm, millimoles,
milliequivalents (Numericals expected).

Primary and Secondary Standards:

Preparation of Standard Solutions, Principle
of Volumetric Analysis.

Acids and Bases: Lowry-Bronsted and

Lewis Concepts. Strong and Weak Acids
and Bases - Ionic Product of Water -
pH,pKa, pKb. Hydrolysis of Salts.

Buffer solutions –Concept of Buffers,Types

of Buffers, Derivation of Henderson
equation for Acidic and Basic buffers,
Buffer action, Buffer capacity(Numericals
expected.)
pH of Buffer Solution.

SEMESTER I

Basic Chemistry-II

COURSE CODE TITLE CREDITS

SBT 102 Bioorganic Chemistry I 2

Isomerism – Types of Isomerism: 15 Lectures
Unit I Constitutional Isomerism (Chain, Position
Stereochemistry and Functional) and Stereoisomerism,
Chirality.

Geometric Isomerism and Optical

Isomerism:Enantiomers, Diastereomers,
and Racemic mixtures Cis-Trans, Threo,
Erythro and Meso isomers.Diastereomerism
(Cis-Trans Isomerism) in Alkenes and
Cycloalkanes (3 and 4 membered ring)

Conformation: Conformations of Ethane.

Difference between Configuration and
Conformation.

Configuration, Asymmetric Carbon Atom,

Stereogenic/ Chiral Centers, Chirality,
 Representation of Configuration by ―Flying
Wedge Formula‖

Projection formulae – Fischer, Newman

and Sawhorse. The Interconversion of the
Formulae.

Titrimetric Analysis: Titration, Titrant, 15 Lectures

Unit II Titrand, End Point, Equivalence Point,
Titrimetry and Gravimetry Titration Error, Indicator, Primary and
Secondary Standards, Characteristics and
examples

Types of Titration –Acid –Base, Redox.

Precipitation, Complexometric Titration.
Acid – Base Titration.-Strong Acid Vs
Strong Base -Theoretical aspects of
Titration Curve and End Point Evaluation.
Theory of Acid –Base Indicators, Choice
and Suitability of Indicators.

Gravimetric Analysis:
Solubility and Precipitation, Factors
affecting Solubility, Nucleation, Particle
Size, Crystal Growth, Colloidal State,
Ageing/Digestion of Precipitate.
Co-Precipitation and Post-Precipitation.
Washing, Drying and Ignition of Precipitate.
(Numericals Expected).
Methods of Seperation 15 Lectures
Unit III Precipitation, Filtration, Distillation and
Analytical Techniques Solvent Extraction.
Analytical Techniques
Chromatography:
Definition, Principles, Types
Introduction to Paper Chromatography,
Thin Layer Chromatography, Column
Chromatography and its
Applications.Colorimetry:
Principle, Beer-Lambert‘s Law,
Measurement of Extinction, Derivation of
E = kcl, Limitations of Beer-Lambart‘s
Law, Filter Selection
 SEMESTER I

Basic Life Sciences-I : Biodiversity and Cell Biology

COURSE CODE TITLE CREDITS

SBT 103 Biodiversity and Cell Biology 2

Origin of Life, Chemical and Biological 15Lectures
Unit I Evolution, Origin of Eukaryotic Cell.
Origin of Life and
Biodiversity Concept of Biodiversity, Taxonomical,
(Animal,Plant, Ecological and Genetic Diversity & its
Microorganisms) Significance

Introduction to Plant Diversity:

Algae, Fungi, Bryophyta, Pteridophyta,
Gymnosperms and Angiosperms (with
one example each)

Introduction to Animal
Diversity:Non-Chordates and
Chordates {with at least one
representative example.)

Introduction to Microbial Diversity

Archaebacteria, Eubacteria, Blue-green
Algae, Actinomycetes, Eumycota-
Habitats, Examples and Applications.
Ultrastructure of Prokaryotic Cell: 15Lectures
Unit II Concept of Cell Shape and Size.Detail
Ultra Structure of Prokaryotic Structure of Slime Layer, Capsule,
and Eukaryotic Cell. Flagella, Pilli, Cell Wall(Gram Positive
and Negative), Cell Membrane,
Cytoplasm and Genetic Material
Storage Bodies and Spores

Ultrastructure of Eukaryotic Cell:

Plasma membrane,
Cytoplasmic Matrix, Microfilaments,
Intermediate Filaments, and
Microtubules
Organelles of the Biosynthetic-
Endoplasmic Reticulum & Golgi
Apparatus.
Lysosome, Endocytosis, Phagocytosis,
Autophagy, Proteasome
Eucaryotic Ribosomes, Mitochondria
and Chloroplasts
 Nucleus –Nuclear Structure, Nucleolus

External Cell Coverings: Cilia And

Flagella
Comparison of Prokaryotic And
Eukaryotic Cells

Bacteria : Classification, Types, 15Lectures

Unit III Morphology (Size, Shape and
Bacteria and Viruses Arrangement) Cultivation of Bacteria.
Reproduction and Growth (Binary
Fission, Conjugation and Endospore
formation) Growth Kinetics, Isolation
and Preservation. Significance of
Bacteria

Viruses :General Characters,

Classification (Plant, Animal and
Bacterial Viruses) Structure and
Characterization of Viruses and
Significance

SEMESTER - I

Basic Life Sciences-II : Microbial Techniques

COURSE CODE TITLE CREDITS

SBT 104 Microbial Techniques 2

Microscopy and Stains 15 lectures
Unit I Microscope- Simple and Compound:
Microscopy and Stains Principle. Parts, Functions and
Applications.
Dark Field and Phase Contrast
Microscope
Stains and Staining Solutions-
Definition of Dye and Chromogen.
Structure of Dye and Chromophore.
Functions of Mordant and Fixative.
Natural and Synthetic Dyes. Simple
Staining, Differential Staining and Acid
Fast Staining with specific examples

Definition : Sterilization and 15 lectures

Unit II Disinfection.
Sterilization Techniques Types and Applications
Dry Heat, Steam under pressure,
 Gases, Radiation and Filteration
Chemical Agents and their Mode of
Action - Aldehydes, Halogens,
Quaternary Ammonium Compounds,
Phenol and Phenolic Compounds,
Heavy Metals, Alcohol, Dyes, and
Detergents

Ideal Disinfectant. Examples of

Disinfectants and Evaluation of
Disinfectant
Nutrition and Cultivation of 15 lectures
Unit III Microorganisms
Nutrition, Cultivation and Nutritional Requirements : Carbon,
Enumeration of Oxygen, Hydrogen, Nitrogen,
Microorganisms Phosphorus, Sulphur and Growth
Factors.
Classification of Different Nutritional
Types of Organisms.
Design and Types of Culture Media.
Simple Medium, Differential, Selective
and Enrichment Media
Concept of Isolation and Methods of
Isolation. Pure Culture Techniques
Growth and Enumeration
Growth Phases, Growth Curve.
Arithmatic Growth and Growth Yield.
Measurement of Growth. Chemostat
and Turbidostat
Enumeration of Microorganisms- Direct
and Indirect Methods
Preservation of Cultures- Principle and
Methods. Cryogenic Preservation
Advantages and Limitations

SEMESTER I

Basic Biotechnology-I : Introduction to Biotechnology

COURSE CODE TITLE CREDITS

SBT 105 Introduction to Biotechnology 2

History & Introduction toBiotechnology 15 lectures
Unit I What is Biotechnology?
Scope and Introduction to Definition of Biotechnology,
Biotechnology Traditional and Modern Biotechnology,
Branches of Biotechnology-
 Plant, Animal Biotechnology, Marine
Biotechnology, Agriculture, Healthcare,
Industrial Biotechnology, Pharmaceuti
cal Biotechnology, Environmental
Biotechnology.
Biotechnology Research in India.
Biotechnology Institutions in India (Pub
lic and Private Sector)
Biotech Suceess Stories
Biotech Policy Initiatives
Biotechnology in context of Developing
World
Public Perception of Biotechnology

Applications of Biotechnology in 15 lectures

Unit II Agriculture : GM Food,
Applications Biotechnology GM Papaya, GM Tomato,
Fungal and Insect Resistant Plants
BT Crops, BT Cotton and BT Brinjal
Pros and Cons
Biotechnological applications in Crop
and Livestock Improvements
Modifications in Plant Quality
Golden Rice,
Molecular Pharming, Plant Based
Vaccines
Ethics in Biotechnology and IPR
Food Biotechnology 15 lectures
Unit III Biotechnological applications
Food and Fermentation in enhancement of Food
Biotechnology Quality
Unit Operation in Food Processing
Quality Factors in Preprocessed Food
Food Deterioration and its Control
Rheology of Food Products
Microbial role in food products Yeast,
Bacterial and other Microorganisms
based process and products
Modern Biotechnological Regulatory
Aspects in Food Industries
Biotechnology and Food - Social
Appraisal
Fermentation Technology
Definition, Applications of
Fermentation Technology
Microbial Fermentations
Overview of Industrial Production of
Chemicals (Acetic Acid, Citric Acid
and Ethanol), Antibiotics,
Enzymes and Beverages
 SEMESTER - I

Basic Biotechnology-II : Molecular Biology

COURSE CODE TITLE CREDITS

SBT 106 Molecular Biology 2

DNA Replication in Prokaryotes and 15 lectures
Unit I Eukaryotes-
Replication Semi-conservative DNA replication,
DNA Polymerases and its role,
E.coli Chromosome Replication,
Bidirectional Replication of Circular
DNA molecules.
Rolling Circle Replication, DNA
Replication in Eukaryotes
DNA Recombination –
Holliday Model for Recombination
Action of Telomerases
Definition and Types of Mutations. 15 lectures
Unit II Mutagenesis and Mutagens.( Examples
Mutation and DNA Repair of Physical, Chemical and Biological
Mutagens)
Types of Point Mutations,
DNA repair,
Photoreversal, Base Excision Repair,
Nucleotide Excision Repair, Mismatch
Repair, SOS Repair and Recombination
Repair.

Experimental evidences for DNA 15 lectures

Unit III and RNA as Genetic Material.
Genetic Engineering Genetic Engineering in E.coli and other
Prokaryotes, Yeast, Fungi and
Mammalian Cells
Cloning Vectors-Plasmids ( pBR 322,
pUC)
Vectors for Plant and Animal Cells,
Shuttle Vectors, YAC Vectors,
Expression Vectors
Enzymes- DNA Polymerases,
Restriction Endonucleases, Ligases,
Reverse Transcriptases, Nucleases,
Terminal Transferases, Phosphatases
Isolation and Purification of DNA
(Genomic, Plasmid) and RNA,,
Identification of Recombinant Clones
Semester – I
Practicals
 SEMESTER – I
Practicals
Basic Chemistry
COURSE CODE TITLE CREDITS Notional
Hours
SBTP 101 Basic Chemistry 2 30 hrs
1. Safety Measures and Practices in Chemistry Laboratory, Working and use of a Digital Balance,
Functioning and Standardization of pH Meter, Optical Activity of a Chemical Compounds by
Polarimeter
2. Preparation of Standard (Molar, Molal and Normal solutions) and Buffer Solutions
Determination of strength of HCl in commercial sample
3. Qualitative Analysis of Inorganic Compounds - Three experiments
4. Characterization of Organic Compounds containing only C, H, O elements (no element test) -
Compounds belonging to the following classes: Carboxylic Acid, Phenol, Aldehyde/Ketone,
Ester, Alcohol, Hydrocarbon and Characterization of Organic Compounds containing C, H, O,
N, S, Halogen Elements (element tests to be done) Compounds belonging to the following
classes: Amine, Amide, Nitro Compounds, Thiamide, Haloalkane, Haloarene
5. To Standardize commercial sample of NaOH using KHP (Potassium hydrogen pthalate) and
sample of HCl using borax.
6. Dissociation Constant of Weak Acids by Incomplete Titration Method using pH Meterand
determination of Acetic acid in Vinegar by Titrimetric Method
7. Determination of the amount of Fe (II) present in the given solution Titrimetrically
8. Determination of amount of NaHCO3 + Na2CO3 in the given solid mixture Titrimetrcially
9. Determination of the amount of Mg (II) present in the given solution complexometrically
10. Determination of percent composition of BaSO4 and NH4Cl in the given mixture
Gravimetrically
11. Separation of Cu, Ni and Fe using Paper Chromatographyand amino acids - paper
chromatography
12. Determination of fluoride ion using Colorimetry and Fe (III) by using Salicylic Acid by
Colorimetric Titration
SEMESTER – I
Practicals
Basic Life Sciences
COURSE CODE TITLE CREDITS Notional
Hours
SBTP 102 Basic Life Science 2 30 hrs
1. Components and workingof Simple, Compound, Dark Field, Fluorescent and Phase Contrast
Microscope
2. Staining of Plant and Animal Tissues using Single and Double Staining Techniques
3. Special Staining Technique for Cell Wall, Capsule and Endospores and Fungal Staining
4. Monochrome Staining, Differential Staining, Gram Staining, and Acid Fast Staining and
Romonowsky Staining
5. Study of Plant, Animal and Microbial Groups with at least one examples from each x 3
6. Study of Photomicrographs of Cell Organelles
7. Sterilization of Laboratory Glassware and Media using Autoclave
8. Preparation of Media- Nutrient broth and Agar, MacConkey Agar, Saborauds Agar
9. Isolation of Organisms : T-streak, Polygon method
10. Enumeration of microorganisms by Serial Dilution, Pour Plate, Spread Plate Method
11. Colony Characteristics of Microorganisms, Enumeration by Breed’s count
12. Growth Curve of E.coli
 SEMESTER – I
Practicals
Basic Biotechnology
COURSE CODE TITLE CREDITS Notional
Hours
SBTP 103 Basic Biotechnology 2 30 hrs
1. Assignment- Study of any branch of biotechnology and its applications
2. Microbial examination of food and detection of Pathogenic Bacteria from Food Samples
3. Isolation of organisms causing Food Spoilage
4. Microscopic determination of Microbial flora from Yoghurt and Lactic Acid Determination
5. Analysis of Milk- Methylene Blue, Resazurin Test, Phosphatase Test
6. Extraction of Caesin from Milk
7. Meat Tenderization using Papain
8. Isolation and purification of DNA (genomic, plasmid)
9. Restriction Digestion
10. Agarose Gel Electrophoresis of the genomic and plasmid DNA
SEMESTER – II
THEORY
 SEMESTER II

Chemistry-I

COURSE CODE TITLE CREDITS

SBT 201 Bioorganic Chemistry 2

Carbohydrates: Structure, Function, 15 lectures
Unit I Classification, Characteristic
Biomolecules: Reactions, Physical and Chemical
Carbohydrates and Lipids Properties, D & L Glyceraldehydes,
structure of Monosaccharide,
Disaccharides, and Polysaccharides.
Isomers of Monosaccharides,
Chemical/Physical Properties of
Carbohydrate, Chemical Reactions for
Detection of Mono., Di and
Polysaccharides,
Lipids: Classification of Lipids,
Properties of Saturated, Unsaturated
Fatty Acids, Rancidity, and
Hydrogenation of Oils
Phospholipids: Lecithin Cephalin,
Plasmalogen
Triacylglycerol-Structure and
Function
Sterols: Cholesterol: Structure and
Function , Lipoproteins: Structure and
Function, Storage Lipids, Structural
Lipids, Action of Phospholipases,
Steroids
Proteins and Amino Acids: 15 lectures
Unit II Classification, Preparation and
Biomolecules: Properties, Isoelectric Point, Peptide
Proteins and Amino Acids Synthesis
Proteins: Classification based on
Structure and Functions, Primary
Structure, N-terminal (Sanger and
Edmans Method) and C-terminal
Analysis (Enzyme) Reactions of
Amino Acids, Sorenson‘ s Titration,
Ninhydrin Test.
Denaturation of protein Structure of
Peptides.
Titration Curve of Amino Acids.
Concept of Isoelectric pH, Zwitter ion.
Glycoproteins
Nucleic Acids: Structure, Function of 15 lectures
Unit III Nucleic Acids, Properties and Types of
 Biomolecules: DNA, RNA. Structure of Purine and
Nucleic Acids Pyrimidine Bases Hydrogen Bonding
between Nitrogeneous Bases in DNA
Differences between DNA and RNA,
Structure of Nucleosides, Nucleotides
and Polynucleotides.

SEMESTER II
Chemistry-II
COURSE CODE TITLE CREDITS

SBT 202 Physical Chemistry 2

Thermodynamics: 15 lectures
Unit I System, Surrounding, Boundaries
Thermodynamics Sign Conventions, State Functions,
Internal Energy and Enthalpy:
Significance, examples, (Numericals
expected.)
Laws of Thermodynamics and its
Limitations, Mathematical expression.
Qualitative discussion of Carnot Cycle
for ideal Gas and Mechanical
Efficiency. Laws of Thermodynamics
as applied to Biochemical Systems.
Concept of Entropy, Entropy for
Isobaric, Isochoric and Isothermal
Processes.
Reaction Kinetics: 15 lectures
Unit II Rate of Reaction, Rate Constant,
Chemical Kinetics Measurement of Reaction Rates Order
& Molecularity of Reaction,
Integrated Rate Equation of First and
Second order reactions (with equal
initial concentration of reactants).
(Numericals expected)
Determination of Order of Reaction
by a) Integration Method b) Graphical
Method c) Ostwald‘s Isolation Method
d) Half Time Method. (Numericals
expected).
Principals of Oxidation & 15 lectures
Unit III Reduction Reactions:
Oxidation Reduction Oxidising and Reducing Agents,
reactions Oxidation Number,
Rules to assign Oxidation Numbers
with examples Ions like
 Oxalate, Permanganate and
Dichromate. Balancing Redox
Reactions by Ion Electron Method
Oxidation, Reduction, Addition and
Substitution & Elimination Reactions.

SEMESTER II
Life Sciences-I
COURSE CODE TITLE CREDITS

SBT 203 Physiology and Ecology 2

Photosynthesis, Intracellular 15 lectures
Unit I Organization of Photosynthetic
Plant Physiology System. Fundamental Reactions of
Photosynthesis, Photosynthetic
Pigments, Role of Light. Hill Reaction
and its Significance, Light Reactions,
Cyclic and Non-Cyclic Photo induced
Electron Flow, Energetics of
Photosynthesis, Photorespiration,
Dark Phase of Photosynthesis, Calvin
Cycle, C-3, C-4 pathways

Introduction to Secondary Metabolites

Physiology of Digestion 15 lectures

Unit II
Animal Physiology Movement of Food and Absorption,
Secretary functions of Alimentary
Canal, Digestion and Absorption,
assimilation in Gut of Mammals

Anatomy of Mammalian Kidney,

Structure of Nephron, Physiology of
Urine Formation and Role of Kidney
in Excretion and Osmoregulation

Physiology of Respiration,
Mechanism of Respiration
Principles of Gaseous Exchange in the
Blood and Body Fluids

Blood and Circulation : Blood

Composition, Structure and Function
of its Constituents
 Blood Coagulation and Anti-
Coagulants
Hemoglobin and its Polymorphism
Regulation of the Circulation
Mechanism and working of Heart in
Human.
Ecology and Biogeography. 15 lectures
Unit III Ecosystems, Definition and
Ecosystem and Interactions Components,
Structure and Function of Ecosystems.
Aquatic and Terrestrial Ecosystems,
Biotic and Abiotic Factors, Trophic
Levels, Food Chain and Food Web,
Ecological Pyramids (Energy,Biomass
and Number)
Nutrient Cycle and Biogeochemical
Cycles: Water, Carbon, Oxygen,
Nitrogen and Sulphur.
Interactions, Commensalism,
Mutualism, Predation and Antibiosis,
Parasitism.

SEMESTER – II
Life Sciences-II
COURSE CODE TITLE CREDITS

SBT 204 Genetics 2

Mendel‘s Laws of Heredity 15 lectures
Unit I Monohybrid Cross: Principle
Genetics Fundamentals of Dominance and Segregation.
Dihybrid Cross: Principle of
Independent Assortment.
Application of Mendel‘s
Principles
Punnett Square.
Mendel‘s Principle in Human
Genetics.
Incomplete Dominance and
Co-dominance.
Multiple Alleles. Allelic series.
Variations among the effect of the
Mutation.
Genotype and Phenotype.
Environmental effect on the
expression of the Human Genes.
Gene Interaction.
Epistasis.
 Genetic analysis in Bacteria- 15 lectures
Unit II Prototrophs, Auxotrophs.
Microbial Genetics Bacteriophages: Lytic and Lysogenic
Development of Phage.
Mechanism of Genetic Exchange in
Bacteria:
Conjugation; Transformation;
Transduction; (Generalized
Transduction, Specialized
Transduction)
Bacterial Transposable Elements.
Genetic Structure of Populations – 15 lectures
Unit III Genotypic Frequencies and Allelic
Population Genetics Frequencies,
Hardy- Weinberg Law and its
assumptions
Genetic Variations in Populations-
Measuring Genetic Variation at
Protein Level and measuring Genetic
Variations at DNA level
Natural Selection.
Genetic Drift
Speciation
Role of Population Genetics in
Conservation Biology

SEMESTER II
Biotechnology-I

COURSE CODE TITLE CREDITS

SBT 205 Tissue Culture & Scientific Writing 2

and Communication Skills
Cell Theory, Concept of Cell Culture, 15
Unit I Cellular Totipotency, lectures
Plant Tissue Culture Organization of Plant Tissue Culture
Laboratory :
Equipments and Instruments
Aseptic Techniques: Washing of
Glassware, Media Sterilization,
Aseptic Workstation, Precautions to
maintain Aseptic Conditions.

Culture Medium: Nutritional

requirements of the explants, PGR‘s and
their in-vitro roles, Media Preparation
Callus Culture Technique: Introduction,
Principle and Protocols
 Basics of Animal Tissue Culture 15
Unit II Introduction lectures
Animal Tissue Culture Cell Culture Techniques,
Equipment and Sterilization
Methodology.
Introduction to Animal Cell Cultures:
Nutritional and Physiological: Growth
Factors and Growth Parameters.
General Metabolism and Growth
Kinetics
Primary Cell Cultures : Establishment
and Maintenance of Primary Cell
Cultures of Adherent and Non-Adherent
Cell Lines with examples.
Application of Cell Cultures
Communication Skills 15
Unit III Introduction to Communication -- lectures
Scientific Writing and Elements, Definitions, Scope of
Communication Skills Communication and Communication as
part of Science
Communication Elements --Verbal and
Non-Verbal Communications.
Principles of Effective Communication,
Oral Presentations
Scientific Reading, Writing &
Presentation
Scientific Writing
Process of Scientific Writing: Thinking,
Planning, Rough Drafts
and Revising Contents.
Introduction to Scientific Reports and
Writings
Compilation of Experimental Data,
Communication Methods in Science,
Examples of Scientific and Unscientific
Writing.
Writing Papers, Reviews, Bibliography
Plagiarism--Introduction to Plagiarism ,
Examples of Plagiarism.

SEMESTER - II
Biotechnology-II
COURSE CODE TITLE CREDITS

SBT 206 Enzymology, Immunology and Biostatics 2

Definition, Classification, 15
Unit I Nomenclature, Chemical Nature, lectures
Enzymes Properties of Enzymes,
Mechanism of Enzyme Action,
Active Sites, Enzyme Specificity,
Effect of pH, Temperature, Substrate
Concentration on Enzyme Activity,
Enzyme Kinetics, Michelis-Menten
Equation,Types of Enzyme
Inhibitions-Competitive,
Uncompetitive, Non-Competitive
Allosteric Modulators CoFactors,
Zymogens,
Overview of Immune Systems, Cell and 15
Unit II Organs involved, T and B cells. lectures
Immunology Innate Immunity, Acquired Immunity,
Local and Herd Immunity, Humoral
and Cellular Immunity - Factors
Influencing and Mechanisms of each.
Antigens and Antibodies: Types of
Antigens, General Properties of
Antigens, Haptens and Superantigens
Discovery and Structure of Antibodies
(Framework region) Classes of
Immunoglobulins, Antigenic
Determinants.
Antigen-Antibody Interactions
Monoclonal Antibodies,
Vaccines (Live, Killed) and Toxoid.
Problems with Traditional Vaccines,
Impact of Biotechnology on Vaccine
Development.
Definition &Importance of Statistics 15
Unit III in Biology lectures
Biostatistics Types of Data, Normal and Frequency
Distribution
Representation of Data and Graphs
(Bar Diagrams, Pie Charts and
Histogram, Polygon and Curve)
Types of Population Sampling
Measures of Central Tendency
(For Raw,Ungroup & Group Data)
Mean
Median
Mode
Measures of Dispersion
Range, Variance, Coefficient of
Variance.
Standard Derivation.
Standard Error.
Semester – II
PRACTICALS
 SEMESTER – II
Practicals
Chemistry
COURSE CODE TITLE CREDITS Notional
Hours
SBTP 201 Chemistry 2 30 hrs
1. Spot test for Carbohydrates, Fats and Proteins and Amino Acids and Nucleic Acids
2. Standardization of Colorimeter and Estimation of Reducing sugar by DNSA method
3. Estimation of Protein by Biuret method and Lowry method
4. Saponification of Fats, Saponification Value of Oil or Fat, Iodine value of Oil and determine
the rate constant for the saponification reaction between ethyl acetate and NaOH by back
titration method
5. To determine enthalpy of dissolution of salt like KNO3
6. Determine the rate constant for hydrolysis of ester using HCl as a catalyst
7. Study the kinetics of reaction between Thiosulphate ion and HCl
8. Study reaction between potassium Persulphate and Potassium Iodide kinetically and hence to
determine order of reaction
9. Study the reaction between NaHSO3 and KMnO4 and balancing the reaction in acidic, alkaline
and neutral medium
10. Study transfer of electrons (Titration of sodium thiosulphate with potassium dichromate)
11. Determination of the volume strength of hydrogen peroxide solution by titration with
standardised potassium permanganate solution
12. Determination of amount of K oxalate and oxalic acid in the given solution Titrimetrcially

SEMESTER – II
Practicals
Life Sciences
COURSE CODE TITLE CREDITS Notional
Hours
USBTP 202 Life Sciences 2 30 hrs
1. Study of Hill‘s reaction
2. Colorimetric study of Absorption Spectrum of Photosynthetic Pigments
3. Movement of Food in Paramoecium- Demonstration.
4. Activity of Salivary Amylase on Starch
5. Analysis of Urine
6. Study of Mammalian Blood, Blood count using Haemocytometer and estimation of
Haemoglobin in Mammalian Blood
7. Study of Human Blood Groups
8. Study of Mammalian Kidney and Heart
9. Problems in Mandelian Genetics
10. Study of Mitosis and Meiosis
11. Study of Karyotypes – Normal Male and Normal Female
12. Study of Interactions Commensalism, Mutualism, Predation and Antibiosis, Parasitism.
 SEMESTER – II
Practicals
Biotechnology
COURSE CODE TITLE CREDITS Notional
Hours
SBTP 203 Biotechnology 2 30 hrs
1. Working and use of various Instruments used in Biotechnology Laboratory (Autoclave, Hot air Oven,
Centrifuge, Incubator, Rotary Shaker, Filter Assembly, LAF, pH meter and Colorimeter)

2. Laboratory Organization and Layout for Plant and Animal Tissue Culture Laboratory
3. Preparation of Stock Solutions and Preparation of Media for PTC
4. Aseptic Transfer Technique, Surface Sterilization and Inoculation for Callus Culture
5. Media Preparation and Sterilization (ATC)
6. Trypsinization of Tissue and Viability Count
7. Qualitative Assay of Enzyme Amylase. Lipase, Protease, Urease, Catalase and Dehydrogenease
8. Enzyme Kinetics : Study of the effect of pH, Temperature on activity of Enzyme
9. Study of Effect of Substrate Concentration on enzyme activity and determination of Vmax and
Km
10. Study of antigen antibody interaction by Ouchterlony method
11. Biometric Analysis for Mean, Median, Mode and Standard Deviation and Data representation
using frequency Polygon, Histogram and Pie Diagram
12. Preparation of review reports of 5 Scientific Papers and Presentation (last 5 years)
 Semester – I and II
Ability Enhancement Course 1 (FC I)
Ability Enhancement Course 2 (FC II)
 SEMESTER I
Ability Enhancement Course 1 (FC I)
Societal Awareness
COURSE CODE TITLE CREDITS

SBT 107 Societal Awareness 2

Understand the multi-cultural diversity of Indian 15 Lectures
Unit I society through its demographic composition:
population distribution according to religion,
Overview of Indian Society caste, and gender; Appreciate the concept of
linguistic diversity in relation to the Indian
situation; Understand regional variations
according to rural, urban and tribal
characteristics; Understanding the concept of
diversity as difference
Concept of Disparity- I 15 Lectures
Unit II Understand the concept of disparity as arising out
of stratification and inequality;
Concept of Disparity Explore the disparities arising out of gender with
special reference to violence against women,
female foeticide (declining sex ratio), and
portrayal of women in media;Appreciate the
inequalities faced by people with disabilities and
understand the issues of people with physical and
mental disabilities
Concept of Disparity-II
Examine inequalities manifested due to the caste
system and inter-group conflicts arising thereof;
Understand inter-group conflicts arising out of
communalism; Examine the causes and effects of
conflicts arising out of regionalism and linguistic
Differences
The Indian Constitution 15 lectures
Unit III Philosophy of the Constitution as set out in the
Preamble; The structure of the Constitution-the
The Indian Constitution and Preamble, Main Body and Schedules;
Significant Aspects of Fundamental Duties of the Indian Citizen;
Political Processes tolerance, peace and communal harmony as
crucial values in strengthening the social fabric of
Indian society; Basic features of the Constitution
Significant Aspects of Political Processes
The party system in Indian politics; Local self-
government in urban and rural areas; the 73rd
and 74th Amendments and their implications for
inclusive politics; Role and significance of women
in politics
 SEMESTER II
Ability Enhancement Course 2 (FC II)
Globalization, Ecology and Sustainable Development
COURSE CODE TITLE CREDITS

SBT 207 Globalization, Ecology and Sustainable 2

Development
Globalisation and Indian Society Understanding the 15 Lectures
Unit I concepts of liberalization, privatization and
Globalisation and Indian globalization; Growth of information technology and
communication and its impact manifested in
Society and
everyday life; Impact of globalization on industry:
Human Rights
changes in employment and increasing migration;
Changes in agrarian sector due to globalization; rise
in corporate farming and increase in farmers’
suicides.
Human Rights
Concept of Human Rights; origin and evolution of
the concept; The Universal Declaration of Human
Rights; Human Rights constituents with special
reference to Fundamental Rights stated in the
Constitution
Ecology and Sustainable Development 15 Lectures
Unit II Importance of Environment Studies in the current
developmental context; Understanding concepts of
Ecology Environment, Ecology and their interconnectedness;
and Environment as natural capital and connection to
Sustainable Development quality of human life; Environmental Degradation
causes and impact on human life; Sustainable
development, concept and components; poverty and
environment
Understanding Stress and Conflict 15 lectures
Unit III Causes of stress and conflict in individuals and
Understanding and Managing society; Agents of socialization and the role played
by them in developing the individual; Significance of
Stress and Conflict in
values, ethics and prejudices in developing the
Contemporary Society
individual; Stereotyping and prejudice as significant
factors in causing conflicts in society. Aggression and
violence as the public expression of conflict
Managing Stress and Conflict in Society
Types of conflicts and use of coping mechanisms for
managing individual stress; Maslow’s theory of self‐
actualisation; Different methods of responding
to conflicts in society; Conflict‐resolution and efforts
towards building peace and harmony in society

Evaluation Scheme
The performance of the learners shall be evaluated into TWO Parts.
The learner’s performance shall be assessed by Internal Assessment with 40 marks & by conducting the
Semester End Examinations with 60 marks .
Practical Training will have Practical Examination for 50 marks at the end of Semester.
The allocation of marks for the Internal Assessment and Semester End Examinations are as follows:-
I. Internal Exam-40 Marks
(i) Test– 15 Marks
(ii) Assignments/Presentations-15 Marks
(iii) Activities - 10 Marks
II. External Examination- 60 Marks
(i) Theory Question Paper Pattern:-
All questions are compulsory.
Question Based on Marks
Q.1 Unit I 15
Q.2 Unit II 15
Q.3 Unit III 15
Q.4 Unit I,II and III 15
 All questions shall be compulsory with internal choice within the questions.
 Each Question may be sub-divided into sub questions as a, b, c, d &e, etc & the
allocation of Marks depends on the weightage of the topic.
III. Practical Examination – 300 marks (50 marks x 6 core papers)
Each Core Subject Carries 50 Marks
Chemistry : 30 marks + 10 marks (Journal)+ 10 marks(Viva-voce)
Life Sciences and Biotechnology : Major (20 marks), Minor (10 marks), Identification /Spots (10
marks), Viva-voce (5 marks), Journal (5 marks)
IV. Ability Enhancement Course
V. Internal Exam-40 Marks
(i)Project– 30 Marks
(ii)Activities-10 Marks
VI. External Examination- 60 Marks
References
st
1. University General Chemistry , 1 edition (2000), C.N. R. Rao, Macmillan Publishers, India ,
st
2. Physical Chemistry University for biological sciences, 1 edition, (2005), Chang R., Science Books,
USA
th
3. Essentials of Physical Chemistry, 24 edition, (2000), B S Bahl, G D Tuli, Arun Bahl, S. Chand
Limited, India.
th
4. Concise Inorganic Chemistry .5 edition (2008), Author: J. D. Lee, John Wiley & Sons, USA.
th
5. Organic Chemistry, 6 edition, (1992), Morrison Robert Thornton, Pearson Publication, Dorling
Kindersley (India Pvt. Ltd.)
6. Jordan, E.L. and Verma P.S. 1978, (i) Chordate Zoology S. Chand & Company Ltd. Ram Nagar. New
Delhi.
7. Jordan, E.L. and Verma P.S. 1978 (ii) Invertebrate Zoology. S. Chand & Company Ltd. Ram Nagar.
New Delhi.
8. Modern Text Book of Zoology: Invertebrates., R.L.Kotpal. Publisher, Rastogi Publications, 2012.
9. Guyton, Text book of Medical Physiology
10. Concise Medical Physiology- Sujit K Chaudhari
11. Human Physiology- Guyton –International Edition
12. Human Anatomy- Marieb
13. Devlin R.M. (1983) - Fundamentals of Plant Physiology (Mac. Millan, New York)
14. Dutta A.C. (2000) A Classbook of Botany (Oxford University Press, UK)
15. Ganguli, Das Dutta (2011) – College Botany Vol I, II and III (New Central Book Agency, Kolkata)
16. Ecology – P.S. Verma and Agarwaal- S. Chand Publications
17. Biotechnology: Environmental Processes- Rehm and Reed- Wiley
18. Molecular Biotechnology- Glick and Pasterman ASM Press
19. Food Microbiology- Frazier
20. Industrial Microbiology- A. H. Patel
21. Industrial Microbiology- L. E. Casida- John Wiley & Sons
st
22. Introductory Biostatistics. 1 edition. (2003), Chap T. Le. John Wiley, USA
23. Methods in Biostatistics- B. K. Mahajan –Jaypee Brothers
th
24. Outlines of Biochemistry: 5 Edition, (2009), Erice Conn & Paul Stumpf ; John Wiley and Sons, USA

th
25. Principles of Biochemistry, 4 edition (1997), Jeffory Zubey, McGraw-Hill College, USA
th
26. Lehninger , Principles of Biochemistry. 5 Edition (2008), David Nelson & Michael Cox, W.H.
Freeman and company, NY.
rd
27. Fundamentals of Biochemistry. 3 Edition (2008), Donald Voet & Judith Voet , John Wiley and
Sons, Inc. USA
th
28. Biochemistry: 7 Edition, (2012), Jeremy Berg, Lubert Stryer, W.H.Freeman and company, NY
rd
29. An Introduction to Practical Biochemistry.3 Edition, (2001), David Plummer, Tata McGraw Hill
Edu.Pvt.Ltd. New Delhi, India
st
30. Biochemical Methods.1 , (1995), S.Sadashivam, A.Manickam, New Age International Publishers,
India
31. Textbook of Biochemistry with Clinical Correlations, 7th Edition, Thomas M. Devlin, January 2010,
st
32. Proteins: biotechnology and biochemistry, 1 edition (2001), Gary Walsch, Wiley, USA
33. Biochemical Calculations, 2nd Ed., (1997) Segel Irvin H., Publisher: John Wiley and Sons, New
York.
34. Enzymes: Biochemistry, Biotechnology & Clinical chemistry, (2001) Palmer Trevor, Publisher:
Horwood Pub. Co., England.
35. Analytical Biochemistry, 3 edition, (1998), David Holmes, H.Peck , Prentice Hall, UK.
36. Culture of Animal cells- Ian Freshney -- John Wiley & Sons
37. Principles and Practice of Animal Tissue culture- Sudha Gangal - University Press
38. Plant Biotechnology- K. G. Ramavat S.Chand Publications
39. Experiments in Plant tissue culture- Dodds and Roberts- Cambridge University Press
th
40. Microbiology–6 Edition (2006), Pelczar M.J., Chan E.C.S., Krieg N.R., The McGraw Hill
Companies Inc. NY
th
41. Presscott‘s Microbiology, 8 edition (2010), Joanne M Willey, Joanne Willey, Linda Sherwood, Linda
M Sherwood, Christopher J Woolverton, Chris Woolverton, McGrawHil Science Enginering, USA
42. Text book of Medical Microbiology, Anantnarayan
43. Microbiology- Frobisher
44. General Principles of Microbiology- Stanier
45. Fundamental Principles of Bacteriology - A. J. Salle McGraw Hill
46. Genetics, (2006) Strickberger MW - (Prentice Hall, India)
47. Human Genetics- A. M. Winchester – MacMillan Press
th
48. Kuby immunology, Judy Owen , Jenni Punt , Sharon Stranford., 7 edition (2012), Freeman and Co.,
NY
st
49. Textbook of basic and clinical immunology, 1 edition (2013), Sudha Gangal and Shubhangi
Sontakke, University Press, India
th
50. Immunology, 7 edition (2006), David Male, Jonathan Brostoff, David Roth, Ivan Roitt, Mosby,
USA.
51. Introduction to Immunology- C V Rao- Narosa Publishing House
52. Cell and Molecular Biology – De Robertis- Lippincott Williams& Wilkins
53. Cell and Molecular Biology- Concepts and Experiments—Karp – Wiley International
54. Essential iGenetics- Peter Russell -Pearson Education

55. Microbial Genetics- Freifelder –Narosa Publishing House

th
56. Genes XI, 11 edition (2012), Benjamin Lewin, Publisher - Jones and Barlett Inc. USA
57. Molecular Biology of the Gene, 6th Edition (2008), James D. Watson, Pearson Education, Inc. and
Dorling Kindersley Publishing, Inc. USA
58. Molecular Biology, 5th Edition (2011), Weaver R., McGraw Hill Science. USA
59. Fundamentals of Molecular Biology, (2009), Pal J.K. and Saroj Ghaskadbi, Oxford University Press.
th
60. Molecular Biology: genes to proteins, 4 edition (2011), Burton E Tropp Jones& Bartlett Learning,
USA