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Cholesterol Bk200 Biobase

This document provides parameters for validating a cholesterol reagent on the BIOBASE BK200 MINI instrument, including item name and number, sample and reagent volumes, normal value ranges, wavelength settings, calibration type and counts. It specifies that the reagent is ready to use, and calibration should use two standards of known concentration in serum/plasma samples, with linear calibration and 3 repeat counts.

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Reactivos Del Valle S.A.S
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448 views2 pages

Cholesterol Bk200 Biobase

This document provides parameters for validating a cholesterol reagent on the BIOBASE BK200 MINI instrument, including item name and number, sample and reagent volumes, normal value ranges, wavelength settings, calibration type and counts. It specifies that the reagent is ready to use, and calibration should use two standards of known concentration in serum/plasma samples, with linear calibration and 3 repeat counts.

Uploaded by

Reactivos Del Valle S.A.S
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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CHOLESTEROL

CHOLESTEROL OXIDASE/PEROXIDASEE

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by
the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

INSTRUMENT: BIOBASE BK200 MINI

REAGENT PREPARATION
Reagents are provided ready to use.

ITEM PARAMETERS

Item Nº * Name CHOLESTEROL


Item Name CHO Sample volum (uL) 3
Min Absorbancy 0 R1 volume (uL) 300
Max Absorbancy 3 R2 Volume (uL)
Normal Low value 0 Sample type SER
Normal High Value 200 Units mg/dL
Lower Value of Test 4
Highest Value of
tests 1000 Prozone check *
Sample Blank yes
Pri Wavelenght 510
Sub Wavelenght 700 Pri-Point
Start Point 34
Decimals Places 2 End Point 35

One Point
Analysis Method End Sub-Point
Start Point -
End point -

Linearity
K 1
b 0
CHOLESTEROL
CHOLESTEROL OXIDASE/PEROXIDASEE

CALIBRATION

Item Name CHO Repeat Counts 3


Two point
Calibration Type linear Sample Type SER
Standard Counts 2 Factor

Standard Absorbance Concentration Cup nº


0 * 0.0 *
1 * * *

* User defined

Version 03/21

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