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Urea - BK200 Biobase

This document provides instrument parameters and settings for analyzing urea levels using a BioBase BK200 Mini instrument. It specifies the test item as urea, with a sample volume of 2uL, R1 reagent volume of 240uL, and R2 reagent volume of 60uL. Normal urea ranges are listed as 12-43 mg/dL. Calibration is a two-point linear calibration using 2 standards of known concentrations analyzed in duplicate.

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Reactivos Del Valle S.A.S
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351 views2 pages

Urea - BK200 Biobase

This document provides instrument parameters and settings for analyzing urea levels using a BioBase BK200 Mini instrument. It specifies the test item as urea, with a sample volume of 2uL, R1 reagent volume of 240uL, and R2 reagent volume of 60uL. Normal urea ranges are listed as 12-43 mg/dL. Calibration is a two-point linear calibration using 2 standards of known concentrations analyzed in duplicate.

Uploaded by

Reactivos Del Valle S.A.S
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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UREA/BUN-UV

UREASE/GLUTAMATE DEHYDROGENASE

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by
the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

INSTRUMENT: BIOBASE BK200 MINI

REAGENT PREPARATION
Reagents are provided ready to use.

ITEM PARAMETERS

Item Nº * Name UREA


Item Name UREA Sample volum (uL) 2
Min Absorbancy 0 R1 volume (uL) 240
Max Absorbancy 3 R2 Volume (uL) 60
Normal Low value 12 Sample type SER
Normal High Value 43 Units mg/dL
Lower Value of Test 3
Highest Value of
tests 300 Prozone check *
Sample Blank yes
Pri Wavelenght 340
Sub Wavelenght Pri-Point
Start Point 22
Decimals Places 1 End Point 26

Analysis Method Fixed Time Sub-Point


Start Point -
End point -

Linearity
K 1
b 0
UREA/BUN-UV
UREASE/GLUTAMATE DEHYDROGENASE

CALIBRATION

Item Name UREA Repeat Counts 3


Two point
Calibration Type linear Sample Type SER
Standard Counts 2 Factor

Standard Absorbance Concentration Cup nº


0 * 0.0 *
1 * * *

* User defined

Version 03/21

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