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Direct Bilirubin: Instrument

This document provides instrument settings and parameters for measuring direct bilirubin levels spectrophotometrically using a Prestige 24i analyzer. It specifies reagent preparation involving combining one vial of Reagent B-D into a Reagent A-D bottle. The calibration involves a linear 6-point calibration. The normal ranges listed are 0-0.30 mg/dL for males and females for both serum and urine samples.

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Enrique Duarte
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98 views1 page

Direct Bilirubin: Instrument

This document provides instrument settings and parameters for measuring direct bilirubin levels spectrophotometrically using a Prestige 24i analyzer. It specifies reagent preparation involving combining one vial of Reagent B-D into a Reagent A-D bottle. The calibration involves a linear 6-point calibration. The normal ranges listed are 0-0.30 mg/dL for males and females for both serum and urine samples.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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DIRECT BILIRUBIN

Spectrophotometric
DIAZOTIZED SULFANILIC

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: PRESTIGE 24i(=LABMAX 240, ZAPHYR 400)

Reagent preparation
Working Reagent: Transfer the contents of one Reagent B-D vial into a Reagent A-D bottle. Mix thoroughly.
Stable for 20 days at 2-8 ºC.

Instrument settings
Item Name BIL-D
CALIBRATION
DATA INFORMATION Type LINEAR
Units mg/dL STANDARD
Decimal 2 #1 * #4
#2 #5
ANALYSIS #3 #6
Type END
Main W. Length 1 546 NORMAL RANGE
Main W. Length 2 660 MALE FEMALE
Method LOW HIGH LOW HIGH
SERUM 0.00 0.30 0.00 0.30
CORR. URINE
Slope Inter
Y= 1 X + 0

ASPIRATION DATA PROCESS ABSORB LIMIT


KIND Double READ START END
VOLUME MAIN 41 43 Low -0.10
SAMPLE 30 µL SUB 28 30 High 0.35
REAGENT 1 VOL 200 µL
REAGENT 2 VOL 50 µL FACTOR ENDPOINT LIMIT 2.5
Third Mix. OFF Blank correction ---
R1 Blank Water Blank LINEAR CHECK (%) 0

Auto Rerun SW On Auto Rerun Condition (Absorbance)


Absorbance Range Lower On
Auto Rerun Range(Result) On Higher On
Lower Higher Prozone Range Off
Serum 0 4.00
Urine --- --- * assigned standard value
Version 0410

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