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CRP Turbidimetry Guide

This document provides guidelines for validating a C-reactive protein (CRP) latex turbidimetry assay on AU 400/640 instruments. It specifies reagent preparation details, instrument settings, calibration parameters, and stability information to facilitate validation of the CRP assay when software or reagent versions change.

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Enrique Duarte
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172 views1 page

CRP Turbidimetry Guide

This document provides guidelines for validating a C-reactive protein (CRP) latex turbidimetry assay on AU 400/640 instruments. It specifies reagent preparation details, instrument settings, calibration parameters, and stability information to facilitate validation of the CRP assay when software or reagent versions change.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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C-REACTIVE PROTEIN (CRP)

Turbidimetry
LATEX

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by
the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instruments: AU 400 / 640


Reagent preparation
Working Reagent: Pour the contents of a Latex vial into a Diluent bottle.
Shake the Latex before pipetting. Mix thoroughly. Stable for 20 days at 2-8 ºC.

Instrument settings
Specific Test Parameters Min OD: Max OD:
Sample: Volume 2 µL Dilution 0 µL L: -0.1 H: 2.5
Reagents: R1 Volume 300 µL Dilution 0 µL Reagent OD limit:
R2 Volume µL First L -0.1 First H 1.5
Wavelength: Pri. 540 Sec. Last L -0.1 Last H 1.5
Method: END
Reaction slope: + Dynamic Range:
Measuring Point 1: First 1 Last 7 L 0 H 150
Measuring Point 2: First 0 Last 0 Correlation Factor:
Linearity: % A 1 B 0
No Lag Time: On-board stability period:

L H
7. None selected
8. Out of range 0 5.0
L H
Panic Value Unit: mg/L Decimal places: 1

Calibration Specific

Calibration Type: AB Formula: Y=AX+B Counts: 2 Process: CONC.

Cal. Nº OD CONC Factor/OD-L Factor/OD-H


Point 1: # * -0.1 2.5

* Enter the assigned standard value


# User defined

Version 0409

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