BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani

Pilani Campus
AUGS/AGSR Division

First semester 2022-2023 Date: October 17th, 2022

COURSE HANDOUT (PART-II)

In addition to part-I (General Handout for all courses appended to the timetable) this portion gives further specific
details regarding the course.
Course No. : BIO F110
Course Title : BIOLOGY LABORATORY
Instructor-in-charge : MEGHANA TARE
Co-Instructors : Sandhya Amol Marathe, B. Vani, Sonia Narwal, Sumukh Thakar, Shobham,
Anirudha Kumar Sahu, Nilanjana Mani, Sayantani Chatterjee, Saurabh Singh, Diksha Sisodiya, Shraddha
Bhatnagar, Shreyas M Iyer, Swarnima Kushwaha, Sonakshi Puri, Ramakrishnan S, Srinjoy Chakraborty,
Smita Dey, Shreya Verma, Anasuya Banerjee, Farhanur Rehman, Lakshay Sharma, Chinmay Nayak,
Muthusaravanan S.

Course Description:
Understanding Biological sciences, analysis and estimation of biomolecules, preparation of temporary slides for
microscopic analysis, study of cell structure and division, investigation of catalytic activity of enzyme, physiology
of plant and animal systems, diversity of living systems.

Scope and Objective of the course:

The major objective of this course is to offer a hands-on experience on fundamental aspects of practical biology. The
student would observe and understand various biological phenomena and also be equipped with some simple
techniques which form the basis of research in biology. The course has been revised to run parallel to General Biology
classes.

Text Book/Manual: Laboratory Manual for Biology, BITS Pilani 2014, revised, 2022.
Reference Book: Simon, E.J. et al: Campbell Essential Biology with Physiology (5th Edition, BITS Pilani custom
edition). Noida: Perason India Education Services Pvt. Ltd., 2015

Experiment Plan

Experiment No. Name of the Experiment Learning Outcome

Experiment-1 Scientific Process Lab: How to perform a Understanding Scientific process, its cyclic nature,
scientific experiment in laboratory. testing a hypothesis
Experiment-2 Microscopy Lab: Introduction to microscopy, Learning about parts of microscope, understanding
different cell types, visualizing different cell domains of life, observing slides under the
microscope
types, preparation of temparory mount
Experiment – 3: DNA Lab: To extract total genomic DNA from Basic knowledge about the genetic material,
cells principle of its isolation.
Experiment – 4: Electrophoresis Lab: Visualizing DNA using gel Understand characterization of DNA based on
electrophoresis molecular weight, using electrophoresis.

Please Do Not Print Unless Necessary

 BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani
Pilani Campus
AUGS/AGSR Division

Experiment – 5: Protein Lab: Measurement of total protein content Proteins: the building blocks; their role in humans;
in the given sample by Lowry’s method. methods to quantify proteins in different samples.
Experiment – 6: Enzyme Lab: To study the effect of the enzyme Properties of an enzyme and chromogenic
lactase on milk detection methods.
Experiment – 7: Physiology Lab: Measurement of haemoglobin Blood group incompatibility, genetics behind blood
content in the human blood and determination of group inheritance, hemoglobin and its importance;
blood group and Rh status. Blood typing.
Experiment – 8: Mitosis Lab: Measurement of mitotic index and Understanding different phases of cell division,
duration of mitosis in the given plant tissue. different factors affecting it and preparation of
Observation of various stages of mitosis through slides to view mitosis in a plant meristematic tissue.
permanent slides.
Experiment – 9: Transport Lab: To study the phenomenon of Effect of tonicity of a solution on different cell
plasmolysis in onion peel. types; osmosis & osmoregulation.
Experiment-10: Transpiration Lab: Preparation of temporary Understanding the role of stomata in controlling
mount of leaf epidermis to study the structure of transpiration in plants. Transpiration rate and
stomata and measurement of transpiration rate factors affecting it.
Experiment – 11: Photosynthesis Lab: To study Photosynthesis in Understanding the process of photosynthesis,
the leaves observing it happen in the lab, importance of
different conditions governing photosynthesis.
Note: The order and number of the experiments listed above may change depending on availability of specific
consumables/equipments for the specific labs.

Evaluation Scheme: Evaluations will be based on laboratory experiments and their manuals and materials provided
by the Instuctors. This scheme is subject to change for administrative reasons.

S. No. Evaluation component Duration Date, time Marks & Nature of

and Venue Weightage (%) component
1. Day to Day Evaluation* (Attendance+ 2 hours Daily Lab 30% NA
Performance)
2. Announced Quiz 30 TBA 24% CB
minutes
3. Comprehensive Exam TBA TBA 46% CB
*Following dress code mentioned in manual is mandatory and will result in partial mark deduction in everyday
evaluations*

Notices: Notices will be displayed on Nalanda.

Make up Policy:
 No make ups will be granted for Daily Labs/quizzes. Genuine cases such as hospitalization, (upon production
of the relevant documents as records/ proof) will be considered upon discussion with team for any make ups
for the final Exam.
 Switching of the labs for make ups/adjustments is not allowed under any circumstances.

Instructor-in-Charge
BIO F110

Please Do Not Print Unless Necessary