INSTRUCTION DIVISION
Course Handout (Semester I, 2017-2018)
                                Course No. : BIO F110
                         Course Title: Biological Laboratory
Instructor in Charge: Anasuya Ganguly
Instructors (faculty): Prof. Utpal Roy, Prof Dibakar Chakrabarty, Prof Rajesh
Mehrotra, Dr Kundan Kumar and Dr Indrani Talukdar
 Course Description:
The spectacular progress over the past two decades in our understanding of biological
processes at the molecular level has been made possible by the coming together of physical,
chemical, biochemical and genetic approaches and by the availability of a wide range of
analytical techniques. This course involves experiments designed to strengthen many
theoretical concepts learnt in the General Biology course.
Course Plan:
Expt No.         Title*
   1         Measurement of total protein content in the given sample.
    2         Measurement of glucose concentration in the given sample
    3        Measurement of mitotic index and duration of mitosis in the given plant tissue.
    4        a. Measurement of Haemoglobin content in human blood
             b. Determination of ABO-Rh Blood Typing.
    5        Quantitation of DNA.
    6        Determination of the Km of phosphatase enzyme.
    7        Separation of chlorophyll pigments by paper chromatography
    8        Study the phenomenon of plasmolysis in onion peel
    9        a. Determination of the level of total cholesterol present in serum.
             b. Determination of Body Mass Index.
   10        Measurement of Blood Pressure.
   11        Observation of permanent slides and comments.
   12        Computational annotation of nucleic acids and proteins.
   13        Tissue location of the endogenous enzyme polyphenol oxidase in the given samples
*For details read “BIOLOGICAL LABORATORY (BIO F110) MANUAL”
Evaluation components:
Components              Duration Marks     Remarks
Lab experiments         2hrs     13X10=130 Total 10 marks per experiment:
                                           Observation/interpretation/result: 5marks
                                           Discipline: 2 mark
                                           Attendance: 3 mark
Viva 1                  10mins   10        19th feb-24th feb
Viva 2                  10mins      10             2nd april-7th april
Mid sem online          30mins      50             3/3/2018
quiz
Comprehensive           1hr         100            30/4/2018 (FN)
Exam
**No Makeup except medical cases with prior intimation from ARC
**Discipline in the laboratory has to be strictly maintained. Punctuality, attendance, good laboratory
practice will be part of evaluation component.
**Covered shoes, full length trousers and lab coats are mandatory and must be worn at the time of
performing an experiment.
Please bring a printout of the results and observation page for each experiment and get it signed after
the experiment is over.
                                                                              INSTRUCTOR-IN-CHARGE
                                                                                  Course No. : BIO F110
                                                                    Course Title : Biological Laboratory