ch13

Student: ___________________________________________________________________________

1.  At least 20% of all amino acids found in histones are the basic amino acids  
A.  glycine or serine.
B.  arginine or lysine.
C.  tryptophane or tyrosine.
D.  valine or praline.
E.  cysteine or isoleucine.

2.  Which of the following histone proteins is the most highly conserved from one organism to another?  
A.  H1
B.  H2A
C.  H2B
D.  H3
E.  H4

3.  Which of the following is the most common type of histone modification?  
A.  lysine e-amino methylation
B.  serine o-phosphorylation
C.  lysine n-phosphorylation
D.  histidine n-phosphorylation
E.  acetylation

4.  One method used by researchers to demonstrate the existence of nucleosome free DNA regions is  
A.  RNA Polymerase Run-off experiments.
B.  Nuclear Run on experiments.
C.  Reporter gene experiments.
D.  Anion Exchange chromatography.
E.  DNase hypersensitivity experiments.

5.  Histone acetylation can occur in the  

A.  nucleus.
B.  cytoplasm.
C.  ER lumen.
D.  nucleus and cytoplasm.
E.  nucleus, cytoplasm, and ER lumen.

 
6.  Which of the following molecules can expedite RNA Pol II elongation through a nucleosome?  
A.  HAT
B.  FACT
C.  IFN-b
D.  ChIP
E.  TRE

7.  Which of the following is the correct order of chromatin folding?  

A.  nucleosome formation, 30 nM fiber formation, radial loop structure
B.  radial loop structure, 30 nM fiber formation, nucleosome formation
C.  30 nM fiber formation, nucleosome formation, radial loop formation
D.  30 nM fiber formation, radial loop formation, nucleosome formation
E.  nucleosome formation, radial loop formation, nucleosome formation

8.  The Histone Code states that the  

A.  primary sequence of the histone proteins never changes over time.
B.  combination of histone modification on a given nucleosome near a gene's control region affects the
efficiency of transcription of that gene.
C.  combination of histone modification on a given nucleosome near a gene's control region affects the
efficiency of transcription of all the nearby genes.
D.  lysines are the only amino acids found in histones that can be acetylated and deacetylated.
E.  histones are found in all living cells.

9.  Some transcription factors, such as ________ can act as both antirepressors and transcription activators.  
A.  GAL4
B.  Rad6
C.  SIR2
D.  RAP1
E.  HP1

10.  Human DNA is folded into ________ that consist of core histones wrapped by DNA.  
________________________________________

11.  The histone protein _______ is more readily removed from chromatin than the other histone proteins and
therefore is not considered part of the core nucleosome.  
________________________________________

 
12.  On average, there is one nucleosme per _______bp of DNA.  
________________________________________

13.  Acetylation of histones occurs on the amino groups of the side chains of the amino acid __________.  
________________________________________

14.  Deacetylation of core histones allows for stronger binding of histones to DNA, which then inhibits
___________________.  
________________________________________

15.  Histone acetyltransferase transfers acetyl groups from _______ to core histones.  
________________________________________

16.  Antirepressors such as GAGA factor are thought to compete with ________ for binding sites on the DNA
template.  
________________________________________

17.  Unacetylated histones tend to ______________ transcription.  

________________________________________

18.  Most eukaryotic cells contain four different kinds of histone proteins.  
True    False

19.  Histones have been shown to have an up regulatory effect on gene activity in vitro.  
True    False

20.  Chromatin remodeling usually occurs on euchromatin, while heterochromatin is very condensed and
genetically inactive.  
True    False

 
21.  Transcription factors help stabilize nucleosomes.  
True    False

22.  If histone acetylation occurs in the cytoplasm, then histone deacetylation will also occur in the cytoplasm.  
True    False

23.  The energy necessary for chromatin remodeling comes from ATP.  
True    False

24.  Histone acetylation usually promotes gene activity.  

True    False

25.  When added to DNA in vitro, unacetylated histones tend to increase transcription from the 5S rRNA
promoter.  
True    False

26.  In nucleosome positioning, activators force the nucleosome to take up positions around the promoter so
RNA polymerase can bind.  
True    False

27.  Chromatin immunoprecipitation (ChIP) can be used to study chromatin methylation in yeast cells.  
True    False

28.  Nucleosome remodeling can either activate or repress transcription.  

True    False

29.  Layborne and Kadonage demonstrated in vitro that by adding increasing amounts of core histones and H1
they could repress transcription from the Drosophila Krüppel gene.  
True    False

 
ch13 Key
 

1.  At least 20% of all amino acids found in histones are the basic amino acids  
A.  glycine or serine.
B.  arginine or lysine.
C.  tryptophane or tyrosine.
D.  valine or praline.
E.  cysteine or isoleucine.

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #1
 

2.  Which of the following histone proteins is the most highly conserved from one organism to another?  
A.  H1
B.  H2A
C.  H2B
D.  H3
E.  H4

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #2
 

3.  Which of the following is the most common type of histone modification?  
A.  lysine e-amino methylation
B.  serine o-phosphorylation
C.  lysine n-phosphorylation
D.  histidine n-phosphorylation
E.  acetylation

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #3
 
4.  One method used by researchers to demonstrate the existence of nucleosome free DNA regions is  
A.  RNA Polymerase Run-off experiments.
B.  Nuclear Run on experiments.
C.  Reporter gene experiments.
D.  Anion Exchange chromatography.
E.  DNase hypersensitivity experiments.

Blooms Level: 5. Evaluate

Section: 13.02
Weaver - Chapter 13 #4
 

5.  Histone acetylation can occur in the  

A.  nucleus.
B.  cytoplasm.
C.  ER lumen.
D.  nucleus and cytoplasm.
E.  nucleus, cytoplasm, and ER lumen.

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #5
 

6.  Which of the following molecules can expedite RNA Pol II elongation through a nucleosome?  
A.  HAT
B.  FACT
C.  IFN-b
D.  ChIP
E.  TRE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #6
 

7.  Which of the following is the correct order of chromatin folding?  

A.  nucleosome formation, 30 nM fiber formation, radial loop structure
B.  radial loop structure, 30 nM fiber formation, nucleosome formation
C.  30 nM fiber formation, nucleosome formation, radial loop formation
D.  30 nM fiber formation, radial loop formation, nucleosome formation
E.  nucleosome formation, radial loop formation, nucleosome formation

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #7
 
8.  The Histone Code states that the  
A.  primary sequence of the histone proteins never changes over time.
B.  combination of histone modification on a given nucleosome near a gene's control region affects the
efficiency of transcription of that gene.
C.  combination of histone modification on a given nucleosome near a gene's control region affects the
efficiency of transcription of all the nearby genes.
D.  lysines are the only amino acids found in histones that can be acetylated and deacetylated.
E.  histones are found in all living cells.

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #8
 

9.  Some transcription factors, such as ________ can act as both antirepressors and transcription activators.  
A.  GAL4
B.  Rad6
C.  SIR2
D.  RAP1
E.  HP1

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #9
 

10.  Human DNA is folded into ________ that consist of core histones wrapped by DNA.  
nucleosomes

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #10
 

11.  The histone protein _______ is more readily removed from chromatin than the other histone proteins and
therefore is not considered part of the core nucleosome.  
H1

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #11
 
12.  On average, there is one nucleosme per _______bp of DNA.  
200

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #12
 

13.  Acetylation of histones occurs on the amino groups of the side chains of the amino acid __________.  
lysine

Blooms Level: 1. Remember

Section: 13.02
Weaver - Chapter 13 #13
 

14.  Deacetylation of core histones allows for stronger binding of histones to DNA, which then inhibits
___________________.  
transcription

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #14
 

15.  Histone acetyltransferase transfers acetyl groups from _______ to core histones.  
acetyl-CoA

Blooms Level: 1. Remember

Section: 13.02
Weaver - Chapter 13 #15
 

16.  Antirepressors such as GAGA factor are thought to compete with ________ for binding sites on the DNA
template.  
histone H1

Blooms Level: 1. Remember

Section: 13.02
Weaver - Chapter 13 #16
 
17.  Unacetylated histones tend to ______________ transcription.  
repress

Blooms Level: 1. Remember

Section: 13.02
Weaver - Chapter 13 #17
 

18.  Most eukaryotic cells contain four different kinds of histone proteins.  
FALSE

Blooms Level: 1. Remember

Section: 13.01
Weaver - Chapter 13 #18
 

19.  Histones have been shown to have an up regulatory effect on gene activity in vitro.  
FALSE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #19
 

20.  Chromatin remodeling usually occurs on euchromatin, while heterochromatin is very condensed and
genetically inactive.  
TRUE

Blooms Level: 1. Remember

Section: 13.02
Weaver - Chapter 13 #20
 

21.  Transcription factors help stabilize nucleosomes.  

FALSE

Blooms Level: 2. Understand

Section: 13.01
Weaver - Chapter 13 #21
 
22.  If histone acetylation occurs in the cytoplasm, then histone deacetylation will also occur in the cytoplasm.  
FALSE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #22
 

23.  The energy necessary for chromatin remodeling comes from ATP.  
TRUE

Blooms Level: 1. Remember

Section: 13.02
Weaver - Chapter 13 #23
 

24.  Histone acetylation usually promotes gene activity.  

FALSE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #24
 

25.  When added to DNA in vitro, unacetylated histones tend to increase transcription from the 5S rRNA
promoter.  
FALSE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #25
 

26.  In nucleosome positioning, activators force the nucleosome to take up positions around the promoter so
RNA polymerase can bind.  
TRUE

Blooms Level: 2. Understand

Section: 13.01
Weaver - Chapter 13 #26
 
27.  Chromatin immunoprecipitation (ChIP) can be used to study chromatin methylation in yeast cells.  
FALSE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #27
 

28.  Nucleosome remodeling can either activate or repress transcription.  

TRUE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #28
 

29.  Layborne and Kadonage demonstrated in vitro that by adding increasing amounts of core histones and H1
they could repress transcription from the Drosophila Krüppel gene.  
TRUE

Blooms Level: 2. Understand

Section: 13.02
Weaver - Chapter 13 #29
 
ch13 Summary

Category # of Questions
Blooms Level: 1. Remember 14
Blooms Level: 2. Understand 14
Blooms Level: 5. Evaluate 1
Section: 13.01 10
Section: 13.02 19
Weaver - Chapter 13 29