NanoEnTek
Dec 2015
Robin Jaehwa Lee
ROBIN LEE
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W h at i s t h e Tre n d ? Seeing is believing
www.dnatube.com www.jove.com
DnaTube is a scientific site providing video based JoVE, the Journal of Visualized Experiments,
studies, lecturers and seminars. is the world's first peer reviewed scientific video journal.
JuLI Stage, Real-Time Cell History Recorder
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Trend: Video Journals are getting popular
Yearly number of video journal from of JoVE
1600
1462 1479
1400
1265
1174 1163
1200 1113 1139
1000 933
863
800 702
630 623
600
375
400
240
200 146 144
41
0
2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 2022
JuLI Stage, Real-Time Cell History Recorder
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W h at i s t h e l i m i tat i o n o f L i ve c e l l sy ste m
Complex
Space needed
Unstable
environment
Expensive
JuLI Stage, Real-Time Cell History Recorder
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W h at i s Re a l T i m e - C e l l H i sto r y Re c o rd e r
Can install inside your incubator
Don’t need Space
Stable environment
Reasonable price
JuLI Stage, Real-Time Cell History Recorder
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W hy Re a l - T i m e C e l l H i sto r y Re c o rd e r
End Point test (Traditional method)
You can get
just final image
Start point History region End point
With Real-Time Cell History Recorder
You can get
Recoding ………
Start point
Full History!
History region End point
JuLI Stage, Real-Time Cell History Recorder
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F l o wc h a r t o f J u L I S ta ge
1 2 3
CHR EDIT STAT
4 Channel Image Capture Image Editor
- Brightness / Contrast Growth Curve
Stitching
- Background Correction
Focus options - Auto Adjust Wound Healing
Z-Stack Movie Maker Attached Cell Counting
- Single
Recorder Whole Intensity Level
- Train
Time-lapse - Matrix Plate Editor
JuLI Stage, Real-Time Cell History Recorder
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S i n g l e i m a ge C a p t u re & M e rge
GFP RFP
DAPI MERGE
JuLI Stage, Real-Time Cell History Recorder
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T i m e - l a p s e ( 9 6 we l l c o n d i t i o n )
JuLI Stage, Real-Time Cell History Recorder
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Fo c u s o p t i o n s
Auto Focus
• Auto Focus : AF works only in Bright field & first position of each wells
ON • All position AF : AF works All position of each wells
• All Channel AF : AF works all channels
• All Cycle AF : AF works all cycles
• Custom AF : If necessary adjust the focus, customer can set the gap of focus value.
Manual Focus
• Customer can set the focus by manually.
• Using the this button, JuLI Stage can memories Z position.
JuLI Stage, Real-Time Cell History Recorder
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Z - sta c k
# of image (Z position) : 3 to 35 image(old ver. 20 image)
Each Depth : 0.5um to 10 um
Maximum cover range 340 um(old ver. 200 um)
JuLI Stage, Real-Time Cell History Recorder
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S t i tc h i n g
* Stitching range : 2 X 2 99 X 99
JuLI Stage, Real-Time Cell History Recorder
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W h o l e we l l S t i tc h i n g
* Stitching range : 2 X 2 99 X 99
Press the Whole button,
automatically stitching range will display
96well plate (whole well) 24well plate (whole well) 6well plate (whole well)
10X Objective Lens 10X Objective Lens 10X Objective Lens
8 X 10 80 images 19 X 25 475 images 40 X 53 2,120 images
JuLI Stage, Real-Time Cell History Recorder
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Po s i t i o n L i st
Available to select the position in more detail.
Even in Time-lapse step can apply this function.
JuLI Stage, Real-Time Cell History Recorder
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Record ( i n P re v i e w )
Can record about preview situation.
Available to record all LED channels.
10 FPS (Frame per Seconds)
Record time : almost 1 hr
Full screen record Crop screen record
JuLI Stage, Real-Time Cell History Recorder
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Record ( H e a r t b e at i n g )
Cardiomyocytes neonataux (France) Cardiomyocytes iPS (France)
JuLI Stage, Real-Time Cell History Recorder
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I m a ge Ed i to r - B a c k g ro u n d C o r re c t i o n ( B r i g ht )
Feature that improves the quality of the image.
Can remove debris or shadows other than the sample.
Before Apply function After Apply function
JuLI Stage, Real-Time Cell History Recorder
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I m a ge Ed i to r – A u to A d j u st ( F l u o re s c e n c e )
Function to solve uncomfortable manual operation.
Only available in case of Fluorescence filed.
Before Apply function After Apply function
JuLI Stage, Real-Time Cell History Recorder
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M o v i e M a ke r
Single
• The ability to create a video file by obtained images.
Train
• The ability to produce one complete video from multiple project, if interruption is
necessary during time-lapse experiments.
• In case of Media change and drug screening assay.
Matrix
• The ability to create a time-lapse of several positions in the single movie.
JuLI Stage, Real-Time Cell History Recorder
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M o v i e m at r i x
JuLI Stage, Real-Time Cell History Recorder
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Confluence & Cell growth cur ve
Before Confluence After Confluence
61.94 %
JuLI Stage, Real-Time Cell History Recorder
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Wound healing growth cur ve
Before analysis
After analysis
JuLI Stage, Real-Time Cell History Recorder
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Atta c h e d C e l l c o u nt i n g ( F l u o re s c e n c e )
GFP image RFP image DAPI image
(nuclear counting)
45 40
JuLI Stage, Real-Time Cell History Recorder
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Atta c h e d C e l l c o u nt i n g ( F l u o re s c e n c e )
Plate Graph Well Graph
Raw Data Export Graph
JuLI Stage, Real-Time Cell History Recorder
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J u L I S c ratc h Syste m
Scratch Mode
Function
Device Analysis SW Specialized Function
Integrated system enables real-time visualization and assessment of cell morphology in scratch
wound assays up to 96-well plates at once – all inside your tissue culture incubator.
JuLI Stage, Real-Time Cell History Recorder
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S c ratc h e r
Creates a uniform thickness scratch line in 96well easily.
Analyze scratch closure in real-time
JuLI Stage, Real-Time Cell History Recorder
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S c ratc h STAT
Relative Wound Density
Wound confluence
Cell confluence
Wound Area Speed Wound Closure Wound Width
Various types of results can be analyzed at one time
: Cell confluence, Wound confluence, Relative wound density, Wound area, Speed wound closure,
Wound width
JuLI Stage, Real-Time Cell History Recorder
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S c ratc h M o d e
Scanning area for Auto positioning
After scanning two areas up and down, automatically
locates the wound area to center.
Wound region Wound Auto Positioning
JuLI Stage, Real-Time Cell History Recorder
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S p h e ro i d STAT
Creates various analysis functions of 96 well single spheroids in real time.
JuLI Stage, Real-Time Cell History Recorder
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S p h e ro i d STAT
Intensity
Area
Spheroid Diameter
Circularity
Volume
Perimeter
Various types of results can be analyzed at one time
: Spheroid Diameter, Fluorescence Intensity, Area, Volume, Circularity, Perimeter.
JuLI Stage, Real-Time Cell History Recorder
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S p h e ro i d STAT
U-2 OS Control CDDP 50 uM CDDP 100 uM
HeLa
SHSY-5Y
A549
293T
JuLI Stage, Real-Time Cell History Recorder
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W h at c a n d o w i t h J u L I S ta ge ?
Cell Proliferation Cellular Localization (Staining Dye)
Stem Cell Monitoring Cell Culture QC
Wound Healing Assay Neurite Outgrowth
Cell Cytotoxicity Monitoring Apoptosis Monitoring
Cell Differentiation Angiogenesis Monitoring
3-D Spheroid Assay Transfection Efficiency
Cell Growth Monitoring Reporter Genes
JuLI Stage, Real-Time Cell History Recorder
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Applications –
C e l l P ro l i fe rat i o n ( N a n o E n Te k )
JuLI Stage, Real-Time Cell History Recorder
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Applications –
A p o p to s i s S c re e n i n g A s s ay ( N a n o E n Te k )
JuLI Stage, Real-Time Cell History Recorder
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Applications –
3 D S p h e ro i d a s s ay ( N a n o E n Te k )
JuLI Stage, Real-Time Cell History Recorder
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Applications –
Wo u n d H e a l i n g ( Ko re a )
GFP & RFP expression cell (NanoEnTek) Fibroblast cell (Cosmetic company)
JuLI Stage, Real-Time Cell History Recorder
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Applications –
C o - C u l t u re M o n i to r i n g ( H u n ga r y & A u st ra l i a )
Osteoclast - Act EGFP (Hungary) Bacterial infection of PBMC (Australia)
JuLI Stage, Real-Time Cell History Recorder
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Applications –
S te m c e l l m o n i to r i n g ( F ra n c e & Ko re a )
Mobility of hMSC (France) Growing of hMSC (Korea)
JuLI Stage, Real-Time Cell History Recorder
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Applications –
E m b r yo n i c c e l l M o n i to r i n g ( I ta l y & J a p a n )
Mouse Embryonic Cell (Italy) Zebra Fish Embryonic Cell (Japan)
Stitching image (2X2)
JuLI Stage, Real-Time Cell History Recorder
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Applications –
V i r u s i n fe c t i o n ( Po l a n d )
infected by HSV-1 Non-infected (Control)
JuLI Stage, Real-Time Cell History Recorder
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Applications –
Tra n sfe c t i o n Ef f i c i e n c y ( 5 x 5 S t i tc h i n g - G e r m a ny )
JuLI Stage, Real-Time Cell History Recorder
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Applications –
3 D A n g i o ge n e s i s A s s ay ( Ko re a )
JuLI Stage, Real-Time Cell History Recorder
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Applications –
K i d n ey m o n i to r i n g ( C a n a d a )
JuLI Stage, Real-Time Cell History Recorder
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Applications –
J e n n a n e u ra l t u b e c l o s u re ( C a n a d a )
JuLI Stage, Real-Time Cell History Recorder
M a g n et b o a rd fo r J u L I S ta ge
For You
JuLI Stage, Real-Time Cell History Recorder
