Table of contents

I. Introduction
1. Live cell imaging
2. Market analysis
3. Conventional microscopy

II. Celloger series

1. Celloger Mini
2. Celloger Nano
3. Celloger Mini Plus

III. Application

IV. Comparison

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I. Introduction
I. Live cell imaging

What is live cell imaging?

Live cell imaging is a method of examining living cells over a period of time using the images
acquired by time-lapse microscopy. Dynamic cellular processes and events are being observed
in real time to study and understand the biological changes of living cells. Live cell imaging has
become an essential method in many fields of life science, especially in research areas such as
cell biology, stem cells, developmental biology, pharmaceuticals, drug discovery, and more.

Importance of live cell imaging

Starting with the discoveries of microscopes in the 16th centuries, there have been
endless desires to peer into objects that cannot or hard to be seen by the naked
eyes. With the development of technology and the introduction of live cell imaging,
it has transformed the way researchers study cells, tissues, proteins, or other
cellular interactions; and became a basic analytical tool in the study of life science.

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I. Market analysis
Global Live Cell Imaging Market

Live Cell Imaging Market Interconnections

Global market trend Research trend
Global live cell imaging market Research on live cell imaging
(*google scholar) Cell
analysis
market
$5.00 60,000 57,400
$3.75
$4.00 50,000 39,600 Life science
$2.90
40,000 instrument
$3.00 $2.36
market
$1.50 30,000 Cell
$2.00 expansion
20,000
6,400 Live Cell market
$1.00 10,000
(in US dollar, billion) Imaging Market
$0.00 -
2018 2020 2023 2026 2000 2010 2020
Cell therapy
technologies
market Cell-based
Key players Key drivers assays
market

The leading players in live cell imaging Key driving factors of live cell imaging
market include BD Biosciences, Bio-Rad, market are rising concerns of cancer, the
BioTek, Carl Zeiss, CytoSmart, Logos need for drug discovery, increasing Cell culture
biosystems, Merck KGaA, Nikon, Olympus, market Microscope High-content
research fundings, and development of screening
market
PerkinElmer, Sartorius, ThermoFisher. advanced instruments. market

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I. Light microscopy

•
•
•
•
Standard optical microscopy used in the laboratory
Can view stained and unstained cells
Only light is used for illumination
Used to see morphology of a cell (not the details of a cell)
VS • Can monitor biological processes & dynamics of cell
• Uses fluorophores that emit light with specific wavelength
when exposed to light
• Sample itself provides light source used to form an image
• Possible to observe specific cells or structure in a cell

Application Application
• Cell Morphology • Wound healing • Cell viability & Cytotoxicity • Co-culture
• Cell growth analysis • Migration • Cell cycle • Transfection with
• Confluency • Proliferation • Apoptosis reporter gene
• Necrosis • Proliferation

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I. Conventional method
Live cell imaging is such a tedious job!

Repeat these steps several times

Load cell samples Culture cells Live cell imaging Acquisition of Analyze cell
cell images images

Labor intensive Prone to human error Difficult to find same position Unstable environment for cell growth

Disadvantages of conventional method

This is the reason why you need Celloger !

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II. Celloger Lineup
Celloger Mini
II-A. Celloger Mini

Diversify your research paradigm with our integrated

live cell imaging system, Celloger Mini

Celloger Mini is a live cell imaging system based on bright-field microscopy with fully motorized stages.
The multi-positioning supported by its motorized stages allows imaging of any position set by the
user, thereby providing more meaningful and reliable results than a single point imaging. Moreover,
intuitive software provides utmost convenience to monitor and analyze live cells in real-time.

This all-around and compact system provides autofocusing, time-lapse imaging, and analytical
software that lets you perform various types of sophisticated research. Celloger Mini is designed
to withstand the temperature and humidity suitable for the growth of cells that makes it
compatible with CO2 incubators.

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II-A. Celloger Mini

Temperature and humidity sensor

Bright field lamp

Objective Lens

220 mm Vessel holder

X-Y Stage

Status light
Green Ready
Blue Scanning
Red PC disconnected

LAN & Power cable

connector (Ethernet)

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II-A. Celloger Mini

1. Cell monitoring inside incubator 2. Compact size

Remotely monitor live cells inside the incubator without disturbing the Compact size makes it straightforward to install and handle, so
environment suitable for cell culture. You can monitor cells in real-time or there is no need for calibration and complicated maintenance
with the time-lapse function, cell images are captured automatically for days procedures. Small-in-size also provides a room for space utilization
or even weeks without having to move the cells in and out of the incubator. inside incubators making it possible for multiple unit installation.

CONVENTIONAL
Vertical : 805mm
Height : 707mm
Horizontal : 226mm CELLOGER MINI
Vertical : 305mm
Height : 220mm
Horizontal : 195mm

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II-A. Celloger Mini

3. Multi-point imaging 4. User-friendly functions

Capturing up to 999 positions Autofocus Ruler

The moving XY stage allows imaging of multiple positions within
the travel range of 117 x 77mm and it is even possible to capture Correct focus Incorrect focus
multiple points within a well. Also, cells cultured in different
vessel types such as well plate, flasks, and dishes can be imaged.

27.5μm

Cell confluency & growth curve

Well-plate Cultureflask Culturedish

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II-A. Celloger Mini
Specification Ordering Information

Dimension 195 x 305 x 220 mm Cat. No. Description

Weight 4.5kg / 9.9lb CRCLG-MB01 CLG Mini, Live cell imaging system (Bright Field, 4X)
Objective Lens 4X CRCLG-MBTF25 Vessel holder, T-Flask A25cm2
Imaging modes Brightfield CRCLG-MBPD35 Vessel holder, Petri dish 35mm
Light source LED CRCLG-MBPD60 Vessel holder, Petri dish 60mm
Camera 1.25MP / 5MP CMOS
Stage Motorized XYZ
Imaging positions Multiple
File export format TIFF, JPEG, PNG, AVI
Culture vessels Flask, dish, well plate, slide
Operating environment 10~40℃, 20~95% humidity
Power requirements 100-240V, ~50/60Hz
Output ports Ethernet
Computer External PC
O/S required Window 10
Processor (recommended) CPU 3G
Storage (recommended) 1TB
Monitor (Recommended) 1920*1080mm
Accessories PoE adapter, ethernet cable, vessel holders, USB memory
Warranty 1 year

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Celloger Nano
II-B. Celloger Nano
Expand your cell-based research through our multifunction live cell imaging system, Celloger Nano.

Examine cell
The NEW super compact and intuitive live cell imaging system, confluence and
healthiness
Celloger Nano - got everything you need to perform your
sophisticate laboratory works. Equipped with exceptional
fluorescence and bright field microscopy, time-lapse imaging,
auto-focusing technology, precise stage controller, and user-
friendly software, accelerates your cell-based research works.
Check cell Remove spent medium
growth and
healthiness
Wash cells

Thaw cells Incubate over night

In vitro cell based
experiment process Trypsinate cells

Transfer to new dish

with fresh medium
Check
detached
Mix cells with cells
Trypan Blue
Resuspend cells in
fresh medium

Freeze cells

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II-B. Celloger Nano

LED status indicator

51mm
Illuminator(Bright Field)

Objective Lens Y axis stage controller

188mm

X axis stage controller

Manual X/Y Stage

LAN port
Fan

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II-B. Celloger Nano

I. Compact system inside incubator II. Precise stage controller

Celloger Nano is a very compact system with a size almost equivalent to half Once the sample is placed at the center of the stage, there is no need to
of A4 paper thus making it suitable for placement inside an incubator. Several move the sample with bare hands. The sample positioning is done with a
Celloger Nano systems can fit into a standard CO2 incubator and are simple touch by using the precise stage controller. This controller facilitates X
connected to one software enabling various experiments at the same time. and Y axis positioning with a distance of -/+ 6mm each axis.

Celloger Mini Celloger Nano

Y axis stage controller

Stage controller

X axis stage controller

305 x 195 x 220 mm 211 x 146 x 188 mm

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II-B. Celloger Nano

III. One-point time-lapse imaging IV. User-friendly function

After placing a sample on the stage and selecting a point to image, time-lapse
setting can be easily done using intuitive Celloger Nano Scan App software. Time- Autofocusing
lapse video (BR, BR+FL merged) can be made with CLG Nano Analysis software.

Intuitive time-lapse scheduling

using Celloger Nano Scan App

Intensity

Analysis tools

Time-lapse video including BR & FL

merged images can be made using
the analysis app.
Confluency Counting (Manual) Ruler

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II-B. Celloger Nano
Specification Ordering Information
Dimension 211 x 146 x188 mm Cat. No. Description
Weight 3.2kg / 7.0lb
CRCLG-NB04 Live cell imaging system (Bright field, 4X)
Objective Lens 4X / 10X
CRCLG-NB10 Live cell imaging system (Bright field, 10X)
Imaging modes Brightfield, Fluorescence (Green / Red)
CRCLG-NBG04 Live cell imaging system (Bright field + Green Fluorescence, 4X)
Green : Excitation (480/30x) / Emission (535/40m)
Fluorescence CRCLG-NBG10 Live cell imaging system (Bright field + Green Fluorescence 10X)
Red: Excitation (510/84x) / Emission (570lp)
Light source LED CRCLG-NBR04 Live cell imaging system (Bright field + Red Fluorescence 4X)

Camera 1.25MP CMOS CRCLG-NBR10 Live cell imaging system (Bright field + Red Fluorescence 10X)

Stage Manual XY, motorized Z

Imaging positions 1
File export format TIFF, AVI (JPEG, PNG)
Culture vessels Flask, dish, well plate, slide
Operating environment 10~40℃, 20~95% humidity
Power requirements 100-240V, ~50/60Hz
Output ports Ethernet
Computer External PC
O/S required Window 10
Processor (recommended) CPU 3G
Storage (recommended) 1TB
Monitor (Recommended) 1920*1080mm
Accessories PoE adapter, ethernet cable, USB memory
Warranty 1 year

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Celloger Mini Plus
II-C. Celloger Mini Plus

Expand your cell discoveries with Celloger Mini Plus,

automated live cell imaging system

Celloger Mini Plus is an automated live cell imaging system that is equipped with an advanced
fluorescence and bright field microscopy, autofocusing and real time multi-position imaging technology.
It provides you all the tools you need to acquire the best quality images and accurate research results.
Various cell-based research work and applications can be done with this all-around system.

Improve your research outcome by performing real-time cell monitoring and analysis using
Celloger Mini Plus. Perform complex research and analysis without taking the cells out of the CO2
incubator, suitable for a long-term cell monitoring with a perfect environment for your cells to grow.

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II-C. Celloger Mini Plus

Objective Lens
LED status indicator

Illuminator
226 mm

(Bright Field)

Vessel holder

LAN port
Fan

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II-C. Celloger Mini Plus

I. Multipoint imaging II. Stable imaging performance

• Using the motorized stages that travel 117mm x 77mm, x and y axis • Celloger Mini Plus doesn’t have a moveable stage but instead, the camera
respectively, multiple points within the travel range can be captured located inside the system moves to capture the images of cell in multiple
following the schedule (intervals, cycles, total time) set by the researcher. positions
• Different kinds of vessels can be used (Well plates, dishes, flasks, slides) • Precise and sensitive fluorescence detection is possible with the integrated
hard-coated optical set and LED filter with more than 50,000-hour lifetime.

48 well plate

24 well plate

12 well plate

6 well plate

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II-C. Celloger Mini Plus

III. Time-lapse imaging IV. Z-stacking

With the Z-stacking function of Celloger Mini Plus, spheroid cells

can be observed using the time-lapse imaging.

Intuitive time-lapse scheduling using

Celloger Mini Plus Scan App.
User can set the total time, cycle, and
intervals of time-lapse imaging

5. Analysis tools
Z position

Time-lapse video of BR & FL merged

images can be made using the Celloger
Mini Plus analysis app.
MOVIE
4.130 4.150 4.170

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II-C. Celloger Mini Plus

Ⅴ. Stitching Ⅵ. User-friendly functions

Capturing multiple images and combining the overlapping parts
enable high-resolution mapping of a large area of a sample. Analysis tools Autofocusing

1 2 3 4 5
One click

6 7 8 9 10

11 12 13 10 11

16 17 18 19 20

21 22 23 24 25

<25 captured images are stitched into a single large image>

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II-C. Celloger Mini Plus
Specification Ordering Information
Dimension 226 x 358 x 215 mm Cat. No. Description
Weight 5.6kg / 12.3lb
CRCLG-MPB04 CLG Mini Plus, Live cell imaging system (Bright Field, 4X)
Objective Lens 4X / 10X
CRCLG-MPB10 CLG Mini Plus, Live cell imaging system (Bright Field, 10X)
Imaging modes Brightfield, Fluorescence (Green / Red)
CRCLG-MPBG04 CLG Mini Plus, Live cell imaging system (Bright Field+Green Fluorescence, 4X)
Green : Excitation (470/40x) / Emission (510lp)
Fluorescence CRCLG-MPBG10 CLG Mini Plus, Live cell imaging system (Bright Field+Green Fluorescence, 10X)
Red: Excitation (510/84x) / Emission (570lp)
Light source LED CRCLG-MPBR04 CLG Mini Plus, Live cell imaging system (Bright Field+Red Fluorescence, 4X)

Camera 5MP CMOS CRCLG-MPBR10 CLG Mini Plus, Live cell imaging system (Bright Field+Red Fluorescence, 10X)

Stage Motorized XYZ CRCLG-MPWPS Vessel holder, Well plate 6~96 (Single)

Imaging positions Multiple CRCLG-MPTFS25 Vessel holder, T-Flask A25cm2 (Single)

File export format TIFF, AVI (JPEG, PNG) CRCLG-MPTFD25 Vessel holder, T-Flask A25cm2 (Dual)

Culture vessels Flask, dish, well plate, slide CRCLG-MPTFS75 Vessel holder, T-Flask A75cm2 (Single)

Operating environment 10~40℃, 20~95% humidity CRCLG-MPPDD35 Vessel holder, Petri dish 35mm (Dual)

Power requirements 100-240V, ~50/60Hz CRCLG-MPPDD60 Vessel holder, Petri dish 60mm (Dual)

Output ports Ethernet CRCLG-MPPDS90 Vessel holder, Petri dish 90/100mm (Single)

Computer External PC
O/S required Window 10
Processor (recommended) CPU 3G
Storage (recommended) 1TB
Monitor (Recommended) 1920*1080mm
Accessories PoE adapter, ethernet cable, USB memory
Warranty 1 year

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III. Applications
III. Applications
Cell morphology varies according to cell types
1. Cell monitoring Epithelial cell(Hela) Macrophage(Raw264.7)

• Studying morphological changes of the cells is an essential method in the cell-based

experiments. The changes in cell morphology occur at every major point in cell cycle and
monitoring these changes in appearance of cells in real time is very important. Epithelial cell(MCF-7) Fibroblast cell(NIH 3T3)
• Researchers can detect the signs of contamination in earlier stage, can determine
senescence stage of the cells, and define the best time for subculture or harvest.

Monitor cell morphology changes over time

Regular assessment of cell

confluency during the cell
culture is essential since it is
the best way to determine the right
time for subculture or harvest.

Migration Cell death Mitosis Ready to Sub-culture

*Taken from Celloger Nano (10X, Green FL) Cell confluency

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III. Applications

2. Wound healing assay (Cell migration)

Culture cells Scratch for “wound”

Wound healing assay is the easiest and fastest way to check cell migration.
When a scratch or space is created in the monolayer of cells, they show the
process of movement to fill in the wound until the wound is entirely healed
with the new healthy cells.
Process of wound healing assay

HeLa cells imaged every 2 hours for 5 days HeLa cell imaged every 10 minutes for 22 hours
*Taken from Celloger Mini *Taken from Celloger Nano (10X, Green FL)

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III. Applications

3. Cell proliferation

• Cell proliferation is to quantify the increasing number of cells over a period of

time to verify that the cells are growing in normal growth process.

• As a method of quantification, number of fluorescent dyed cells or cell

confluency is measured. In other words, a graph of cell number or confluency
changes over time is mainly used as the result of proliferation.

Confluency Time-lapse video

30% 50%

HeLa cell imaged every 1 hour for 23 hours

*Taken from Celloger Mini Plus (4X, Red FL)
60% 70%

NIH 3T3 cell imaged every 15 minutes for 56 hours

*Taken from Celloger Nano (10X, Green FL)

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III. Applications
Analysis software video

4. Cytotoxicity assay
• Cytotoxicity refers to the state of being toxic to cells. Suppression of cell growth
and division, cell lysis and apoptosis occur when stimulating activities such as
toxic substances or environmental changes affect the cell health. Cytotoxicity
assay is a way that compares these occurrences with the control group.

• Depending on the purpose of the experiment, the result of Cytotoxicity could be

cell death rate by time, by drug concentration, and by drug type.

Bright-field Fluorescence Time-lapse video Confluency graph

HeLa cell imaged every 1 hour for 48 hours HeLa cell imaged every 1 hour for 71 hours
*Taken from Celloger Nano (4X, Green FL) *Taken from Celloger Nano (4X, Red FL)

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III. Applications

5. Transfection efficiency assessment

• Transfection is a method of inserting genes into eukaryotic cells and genes DNA of your favorite protein Your favorite protein
DNA of GFP
called ‘reporter gene’ such as GFP allow easy analysis of gene expressed after GFP
the transfection. DNA RNA Protein

• Transfection efficiency is measured to enhance the delivery of gene into Inside the cell
cultured cells without affecting the cell viability.

Bright-field Fluorescence

HeLa cell imaged every 2 hour for 44 hours

*Taken from Celloger Nano (4X, Green FL)

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III. Applications

6. Reactive oxygen species (ROS) detection

Control Hydrogen peroxide 200uM

Cells generate ROS as a by-product in the aerobic metabolism process. In

order to prevent the excessive generation of ROS, antioxidative defense
mechanism exists in a cell to maintain cellular redox homeostasis. But
excessive ROS causes oxidative stress, resulting in various diseases Bright field
including cancer. image

Green
fluorescence
image

HeLa cells stained with DCF-DA 20uM taken with Celloger Nano 4X, Green FL

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III. Applications

7. Coculture monitoring 8. Apoptosis

• Coculture monitoring is analysis of cell-to-cell contact and it is the real time Apoptosis is the process of programmed cell death where processes
observation of changes in morphology caused by the interactions of two or such as membrane blebbing, cell shrinkage and nuclear fragmentation
more cells using live cell imaging. occur. In this process, the enzyme called caspase is activated to mediate
this reaction in the cell.
• In order to distinguish the two cells, one cell is labelled with fluorescence and
then cultured with other types of cell.
0hr 6hr 10hr

Bright field

Fluorescence

NIH3T3 & MCF-7 cells taken with Hela cells imaged every 30 minutes for 10 hours
Celloger Nano 4X, Green FL *Taken from Celloger Nano (4X, Green FL)

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IV. Comparison
IV. Celloger series comparison
Celloger Mini Celloger Nano Celloger Mini Plus

Bright-field imaging O O O

Fluorescence imaging X O O

Magnification 4X 4X / 10X 4X / 10X

4X: 1.4 x 1.0 mm 4X: 1.4 x 1.0 mm

Field of view 1.4 x 1.0 mm
10X: 0.5 x 0.4 mm 10X: 0.5 x 0.4 mm

O X O
Automatic stage
(Moving stage) (Stage controller) (Moving optics)

Travel range 75 x 117 mm 12 x 12mm (Manual) 75 x 117 mm

Autofocusing O O O

Manual focusing O O O

Z-stacking X O (Manual) O

Stitching X X O

Multi-point imaging O X O

Time-lapse imaging O O O

Movie maker O O O

Dimension (LxWxH) 305 x 195 x 220 mm 211 x 146 x 188 mm 358 x 226 x 215 mm

Weight 4.5kg 3.2kg 5.6kg

End user price $19,500 $9,500 $29,000

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Thank you
End of Document

Curiosis Inc.

4F 10, Teheran-ro 38-gil, Gangnam-gu, Seoul 06221, South Korea I T 82 2 508 5237 I F 82 2 508 5246 I sales@curiosis.com
www.curiosis.com