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Ecg Product Info

(-)-Epicatechin gallate is a polyphenol compound found naturally in green tea. It has been shown to have antioxidant properties and inhibit factors involved in cell growth and blood vessel formation. Specifically, studies have found that (-)-epicatechin gallate inhibits tyrosine phosphorylation of platelet-derived growth factor and vascular endothelial growth factor receptors in vascular smooth muscle cells. Methods for purifying and analyzing (-)-epicatechin gallate using techniques like liquid-liquid partitioning, high-speed countercurrent chromatography, gel chromatography, microemulsion electrokinetic chromatography, and HPLC have been described.

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97 views1 page

Ecg Product Info

(-)-Epicatechin gallate is a polyphenol compound found naturally in green tea. It has been shown to have antioxidant properties and inhibit factors involved in cell growth and blood vessel formation. Specifically, studies have found that (-)-epicatechin gallate inhibits tyrosine phosphorylation of platelet-derived growth factor and vascular endothelial growth factor receptors in vascular smooth muscle cells. Methods for purifying and analyzing (-)-epicatechin gallate using techniques like liquid-liquid partitioning, high-speed countercurrent chromatography, gel chromatography, microemulsion electrokinetic chromatography, and HPLC have been described.

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© Attribution Non-Commercial (BY-NC)
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(−)-Epicatechin gallate

from green tea

Product Number E3893


Storage Temperature 2-8 °C

Product Description References


Molecular Formula: C22H18 O10 1. Nakagawa, T., and Yokozawa, T., Direct
Molecular Weight: 442.4 scavenging of nitric oxide and superoxide by
CAS Number: 1257-08-5 green tea. Food Chem. Toxicol., 40(12), 1745-
Synonym: (2R,3R)-2-(3,4-dihydroxyphenyl)- 1750 (2002).
3,4-dihydro-1(2H)-benzopyran-3,5,7-triol 3-(3, 2. Anderson, R. F., et al., Green tea catechins
4,5-trihydroxybenzoate) partially protect DNA from • OH radical-induced
strand breaks and base damage through fast
(-)-Epicatechin gallate is a polyphenol compound, or chemical repair of DNA radicals. Carcinogenesis,
catechin, that occurs naturally in tea (Camellia 22(8), 1189-1193 (2001).
sinensis). This compound and other tea polyphenols 3. Sachinidis, A., et al., Inhibition of the PDGF
1,2
have been studied for their antioxidant properties. β-receptor tyrosine phosphorylation and its
downstream intracellular signal transduction
Epicatechin gallate has been shown to inhibit tyrosine pathway in rat and human vascular smooth
phosphorylation of platelet-derived growth factor Rβ in muscle cells by different catechins. FASEB J.,
rat and human vascular smooth muscle cells and of 16(8), 893-895 (2002).
vascular endothelial growth factor receptor 2 (VEGF 4. Lamy, S., et al., Green tea catechins inhibit
3,4
R2). A study of the action of epicatechin gallate and vascular endothelial growth factor receptor
other compounds on plasma coagulation by phosphorylation. Cancer Res., 62(2), 381-385
5
Staphylococcus aureus has been described. (2002).
5. Akiyama, H., et al., Antibacterial action of several
A protocol for the purification of epicatechin gallate tannins against Staphylococcus aureus. J.
that uses liquid-liquid partitioning, high-speed Antimicrob. Chemother., 48(4), 487-491 (2001).
countercurrent chromatography, and gel 6. Baumann, D., et al., A simple isolation method for
6
chromatography has been published. The analysis of the major catechins in green tea using high-speed
epicatechin gallate and other green tea constituents by countercurrent chromatography. J. Nat. Prod.,
microemulsion electrokinetic chromatography has 64(3), 353-355 (2001).
7
been described. An HPLC/electrochemical detection 7. Pomponio, R., et al., Microemulsion electrokinetic
technique for the analysis of epicatechin gallate and chromatography for the analysis of green tea
8
other tea catechins has been reported. An HPLC/MS catechins: Effect of the cosurfactant on the
study has analyzed the presence of epicatechin separation selectivity. Electrophoresis, 24(10),
9
gallate and other polyphenols in grapes. 1658-1667 (2003).
8. Sano, M., et al., Simultaneous determination of
Precautions and Disclaimer twelve tea catechins by high-performance liquid
For Laboratory Use Only. Not for drug, household or chromatography with electrochemical detection.
other uses. Analyst, 126(6), 816-820 (2001).
9. Wu, Q., et al., Determination of proanthocyanidins
Preparation Instructions in grape products by liquid chromatography/mass
This product is soluble in water (5 mg/ml), with heat spectrometric detection under low collision energy.
and sonication as needed, yielding a clear, colorless Anal. Chem., 75(10), 2440-2444 (2003).
solution.
GCY / RXR 5/06

Sigma brand products are sold through Sigma-Aldrich, Inc.


Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser
must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of
the invoice or packing slip.

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