Icomed
Icomed
OPERATING MANUAL
       CO2 INCUBATOR ICOmed
                   ICO50med
                  ICO105med
                  ICO150med
                  ICO240med
Manufacturer and customer service
MEMMERT GmbH + Co. KG
Willi-Memmert-Straße 90-96
91186 Büchenbach
Germany
Phone:              +49 (0)9122 925-0
Fax:                +49 (0)9122 14585
E-mail:             sales@memmert.com
Internet:           www.memmert.com
Customer service:
Service hotline:    +49 (0)9171 9792 911
Service fax:        +49 (0)9171 9792 979
E-mail:             service@memmert.com
For service enquiries, please always specify the appliance number on the nameplate (see page
13).
Shipping address for repairs:
Memmert GmbH + Co. KG
Customer service
Willi-Memmert-Str. 90-96
DE-91186 Büchenbach
Germany
Please contact our customer service before sending appliances for repair or before returning
equipment, otherwise, we have to refuse acceptance of the shipment.
Versions
The appliances are available in different configurations and sizes. If specific equipment fea-
tures or functions are available only for certain configurations, this is indicated at the relevant
points in this manual.
The functions described in this manual refer to the latest firmware version.
Due to individual configurations and sizes, illustrations in this manual may be slightly different
from the actual appearance. Function and operation are identical.
Contents
1.     For your safety                                                                                                                        6
1.1    Terms and signs used........................................................................................................... 6
1.2    Product safety and dangers ................................................................................................ 6
1.3    Requirements of the operating personnel .......................................................................... 8
1.4    Responsibility of the owner................................................................................................. 8
1.5    Intended use ........................................................................................................................ 8
1.6    Changes and conversions.................................................................................................... 9
1.7    Behaviour in case of malfunctions and irregularities .......................................................... 9
1.8    What to do in case of accidents .......................................................................................... 9
1.9    Switching off the appliance in an emergency .................................................................. 10
2.     Construction and description                                                                                                         11
2.1    Construction ...................................................................................................................... 11
2.2    Description and function................................................................................................... 11
2.3    Material.............................................................................................................................. 12
2.4    Electrical equipment .......................................................................................................... 12
2.5    Connections and interfaces ............................................................................................... 12
2.6    Designation (nameplate) ................................................................................................... 13
2.7    Technical data .................................................................................................................... 14
2.8    Applied directives and standards ...................................................................................... 15
2.9    Declaration of conformity ................................................................................................. 15
2.10   Note in accordance with Medical Devices Directive ......................................................... 15
2.11   Ambient conditions ........................................................................................................... 16
2.12   Scope of delivery ............................................................................................................... 16
2.13   Optional accessories .......................................................................................................... 16
3.     Delivery, transport and setting up                                                                                                   17
3.1    For your safety ................................................................................................................... 17
3.2    Delivery .............................................................................................................................. 18
3.3    Transport............................................................................................................................ 18
3.4    Unpacking ......................................................................................................................... 18
3.5    Storage after delivery ........................................................................................................ 18
3.6    Setting up .......................................................................................................................... 19
4.     Putting into operation                                                                                                               21
4.1    Connect the appliance to the power supply..................................................................... 21
4.2    Establishing water supply.................................................................................................. 21
4.3    CO2 and N2 connection...................................................................................................... 22
4.4    Switching on...................................................................................................................... 23
5.     Operation and control                                                                                                                24
5.1    For your safety ................................................................................................................... 24
5.2    Operating personnel.......................................................................................................... 24
5.3    Opening the door .............................................................................................................. 25
5.4    Loading the appliance ....................................................................................................... 26
5.5    Operating the appliance.................................................................................................... 26
5.6    Monitoring function .......................................................................................................... 33
5.7    Graph ................................................................................................................................. 39
5.8    Sterilising the appliance ................................................................................................... 40
5.9    Ending operation............................................................................................................... 41
      Do not tilt     Disconnect the     Wear gloves         Wear safety      Observe informa-
                        mains plug                             boots           tion in separate
                                                                                   manual
                                        Important or
                                            useful
    Information on       First aid:    additional infor-
        first aid     Rinse eyes out       mation
        WARNING
          After removing covers, live parts may be exposed. You may receive
          an electric shock if you touch these parts. Disconnect the mains plug
          before removing any covers. Only electrical engineers may work on
          the electrical equipment of the appliances.
        WARNING
          When loading the appliance with an unsuitable load, poisonous or
          explosive vapours or gases may be produced. This could cause the
          appliance to explode, and people could be severely injured or poi-
          soned. The appliance may not be loaded with materials / test objects
          that release toxic or explosive vapours when heated up (see also
          chapter Intended use on page 8).
        WARNING
          Leaving the door open during operation can cause the appliance to
          overheat or pose a fire hazard. Do not leave the door open during
          operation.
        WARNING
          Gas bottles may burst or explode at high temperatures. Keep the
          gas bottles away from open flames. Store gas bottles below 50 °C
          and ensure that the location is always well ventilated. Prevent water
          from penetrating as well as backflow into the gas bottles. It is es-
          sential that you read the safety notes and instructions of the gas
          supplier.
        WARNING
          Condensation might cause a short circuit. After transport or storage
          at high humidity conditions, the appliance shall be stored unpacked
          at normal conditions for at least 24 hours. During this period of time
          the appliance shall not be connected to the power supply.
         CAUTION
          Danger of suffocation. CO2 and N2 can have a suffocating effect
          in high concentrations. In normal mode, the appliance emits small
          amounts of CO2 and N2 to its surroundings. You should therefore
          ensure that the room in which it is installed is properly ventilated.
          A ventilation rate of 250 m³ / h is required. Always close the stop
          valve or pressure reducer on the gas bottles if the appliance is not in
          operation.
         CAUTION
          High concentrations of CO2 can cause cold burns or frostbite. Avoid
          contact with CO2 gas to the eyes and skin.
CO2 and N2 are not dangerous substances in terms of the German Hazardous Substances
Ordinance (GefStoffV). You should nevertheless familiarise yourself with the applicable safety
regulations prior to handling such gas bottles.
1.5.1 Intended purpose as per Directive 93/42/EEC (Council Directive to unify legal
      provisions of the member states concerning medical devices)
The CO2 incubator ICOmed is used to generate and maintain constant ambient conditions for
the in-vitro fertilisation (IVF) application field, especially for the incubation of oocytes, sperma-
tozoa and zygotes in special culture dishes for IVF application as well as for gene expression
and the biosynthesis of RNA and proteins.
           Rinse eyes out with water for at least 15 minutes. In case of cold burns, rinse with
           water for at least 15 minutes. Cover over in a sterile way. Call a doctor.
                                                            Fig. 1
                                                            Switch off the appliance by press-
                                                            ing the main switch
                                                                            7
      1
      2
      3
      4
2.3 Material
For the outer housing, MEMMERT uses stainless steel (Mat.No. 1.4016 – ASTM 430) and for
the interior, stainless steel (Mat.No. 1.4301 – ASTM 304) is used, which stands out through
its high stability, optimal hygienic properties and corrosion resistance to many (but not all!)
chemical compounds (caution for example with chlorine compounds).
The chamber load for the appliance must be carefully checked for chemical compatibility with
the materials mentioned. A material resistance table can be requested from the manufacturer.
Ethernet interface
Via Ethernet interface, the appliance can
be connected to a network, so that you
can transfer programmes created with the
AtmoCONTROL software to the appliance and
read out protocols. The Ethernet interface is
located on the rear of the appliance (Fig. 4).
For identification purposes, each appliance con-
nected must have its own unique IP address.
Setting the IP address is described on page
49.
                                                    Fig. 4      Ethernet interface
           You will find a description of how to transfer programmes via Ethernet in the en-
           closed AtmoCONTROL manual.
With an optional USB to Ethernet converter, the appliance can be directly connected to a com-
puter / laptop (see Optional accessories on page 16).
D F
                                            E
                                       B
                                                                                       56
                   A
Fig. 6    Dimensions
2.8 Applied directives and standards
► Council Directive 93/42/EEC from 14 June 1993 on medical devices with amendments.
  Standards complied with: EN 60601-1-2, EN 61010-1, EN 61010-2-010
► Directive 2011/65/EU of the European Parliament and of the Council on the restriction of
  the use of certain hazardous substances in electrical and electronic equipment
      Ambient temperature                     10 °C to 35 °C
      Humidity rh                             max. 70 %, non-condensing
      Overvoltage category                    II
      Pollution degree                        2
      Altitude of installation                max. 2,000 m above sea level
► The appliance may not be used in areas where there is a risk of explosion. The ambient air
  must not contain any explosive dusts, gases, vapours or gas-air mixtures. The appliance is
  not explosion-proof.
► Heavy dust production or aggressive vapours in the vicinity of the appliance could lead to
  sedimentation in the interior and, as a consequence, could result in short circuits or dam-
  age to electrical parts. For this reason, sufficient measures to prevent large clouds of dust
  or aggressive vapours from developing should be taken.
          Because of the heavy weight of the appliance, you could injure your-
          self if you try to lift it. To carry appliances of size 50, at least two
          persons are needed; for appliances of the sizes 105 and 150, four
          people are needed. Appliances larger than that may not be carried
          but must be transported with a manual pallet jack or forklift truck.
                   50               105              150                 240
CAUTION
3.2 Delivery
The appliance is packed in cardboard and is delivered on a wooden palette.
3.3 Transport
The appliance can be transported in the following ways:
► With a forklift truck; move the forks of the truck entirely under the pallet.
► On a manual pallet jack
3.4 Unpacking
         NOTICE
► To avoid damage, do not unpack the appliance until you reach the installation site.
Remove the cardboard packaging by pulling it upwards or carefully cutting along an edge.
3.4.1 Checking for completeness and transport damage
► Check the delivery note to ensure that the delivery is complete.
► Check the appliance for damage.
If you notice deviations from the delivery note, damage or irregularities, do not put the appli-
ance into operation but inform the haulage company and the manufacturer.
3.4.2 Removing the transport protection
Remove the transport protection. It is located between the door hinge, door and frame and
has to be removed after opening the door.
3.4.3 Disposing of packaging material
Dispose of the packaging material (cardboard, wood, foil) in accordance with the applicable
disposal regulations for the respective material in your country.
3.6 Setting up
3.6.1 Preconditions
The installation site must be flat and horizontal and must be able to reliably bear the weight
of the appliance (see Technical data on page 14). Do not place the appliance on a flamma-
ble surface.
The appliance emits small amounts of CO2 and N2 to its surroundings during operation. There-
fore, the installation site must be ventilated.
Depending on the model (see nameplate), a 230 V or 115 V power connection must be avail-
able at the installation site.
The distance between the wall and the rear of the appliance must be at least 15 cm. The clear-
ance from the ceiling must not be less than 20 cm and the side clearance from walls or nearby
appliances must not be less than 5 cm (Fig. 8). Sufficient air circulation in the vicinity of the
appliance must be guaranteed at all times.
                                      FP
The first time the appliance is operated, it must not be left unattended until it has reached the
steady state.
4.1 Connect the appliance to the power supply
         WARNING
           Condensation might cause a short circuit. After transport or storage
           at high humidity conditions, the appliance shall be stored unpacked
           at normal conditions for at least 24 hours. During this period of time
           the appliance shall not be connected to the power supply.
Observe the country-specific regulations when making
connections (e.g. in Germany: DIN VDE 0100 with earth
leakage circuit breaker). Observe the connection and
power ratings (see nameplate and "Technical Data" on
page 14). Make sure to establish a safe PE conductor
connection.
Plug the provided power cable into the rear of the appli-        Fig. 9     Connect the power
ance and connect it to the power supply (Fig. 9). Place the cable to the rear of the appliance
power cable so that
► it is easily accessible at all times and can be pulled off quickly, for example in case of inter-
    ference or an emergency;
► it does not represent a trip hazard;
► it cannot come into contact with any hot parts.
4.2 Establishing water supply
4.2.1 Water specifications
For steam creation, use only: demineralised/desalinated water (Aqua Dem) with a conduc-
tivity ≤ 10 μS/cm. Otherwise, possible calcification in the steam generators, steam piping
and steam generator pumps could affect the correct functioning of the appliance. Only use
chlorine-free water with a pH value of > 5 and < 7.
4.2.2 For appliances with passive humidity control: inserting the water tray
Mount the provided sealing lip to the narrow side of the water tray (Fig. 10) and fill 1.5 cm to
2 cm of water into the tray (for specifications see section 4.2.1).
Place the water tray with the attached sealing lip in the centre of the appliance floor and
carefully push it towards the rear panel until the sealing lip is completely under the ventilation
opening, touching the rear panel along its entire length (Fig. 11). The sealing lip magnetically
adheres to the rear panel and returns the water condensing on the humidity limiter to the
water tray.
          NOTICE
Important: make sure not to spill any water and make sure that no water flows past the water
tray and onto the floor, as this would cause the humidity to exceed the maximum values.
Fig. 10    Attach the sealing lip                  Fig. 11    Push the water tray to the rear
                                                   panel
4.2.3 For appliances with active humidity control: Fill up and connect the water
      tank
Fill the supplied water tank with water and use the enclosed tube to connect it to the “H2O”
connection on the rear of the chamber (Fig. 12).
4.3 CO2 and N2 connection
          WARNING
           Danger of explosion and poisoning when introducing gases/sub-
           stances other than CO2 and N2. Only carbon dioxide (CO2) and ni-
           trogen (N2) may be introduced into the appliance through the gas
           connections on the rear of the appliance.
          WARNING
           Gas bottles may burst or explode at high temperatures. Keep the gas
           bottles away from open flames. Do not store gas bottles at or above
           50 °C and ensure that the location is always well-ventilated. Prevent
           water from penetrating as well as backflow into the gas bottles. It is
           essential that you read the safety notes and instructions of the gas
           supplier.
          CAUTION
            Danger of suffocation. CO2 and N2 can have a suffocating effect
            in high concentrations. In normal mode, the appliance emits small
            amounts of CO2 and N2 to its surroundings. You should therefore
            ensure that the room in which it is installed is properly ventilated.
            A ventilation rate of 250 m³ / h is required. Always close the stop
            valve or pressure reducer on the gas bottles if the appliance is not in
            operation.
          CAUTION
            High concentrations of CO2 can cause cold burns or frostbite. Avoid
            contact with CO2 gas to the eyes and skin.
                         ON
                         O N
          Leaving the door open during operation can cause the appliance to
          overheat or pose a fire hazard. Do not leave the door open during
          operation.
WARNING
          Gas bottles may burst or explode at high temperatures. Keep the gas
          bottles away from open flames. Do not store gas bottles at or above
          50 °C and ensure that the location is always well-ventilated. Prevent
          water from penetrating as well as backflow into the gas bottles. It is
          essential that you read the safety notes and instructions of the gas
          supplier.
         CAUTION
          Danger of suffocation. CO2 and N2 can have a suffocating effect
          in high concentrations. In normal mode, the appliance emits small
          amounts of CO2 and N2 to its surroundings. You should therefore
          ensure that the room in which it is installed is properly ventilated.
          A ventilation rate of 250 m³ / h is required. Always close the stop
          valve or pressure reducer on the gas bottles if the appliance is not in
          operation.
CAUTION
A B
NOTICE
► Check the chamber load for chemical compatibility with the materials of the appliance (see
     page 12).
Insert the sliding steel grids or sliding shelves. The maximum number or grids / shelves and the
load capacity are specified in the technical data overview from page 14.
Load the chamber leaving
enough space between the
items so that proper air
circulation in the interior is
guaranteed. Do not place any
of the chamber load on the
bottom, touching the side
walls or right below the
ceiling of the chamber (Fig.
15, see also the "correct
loading" sticker on the
appliance).
In case of improper loading
(not enough space between            Fig. 15   Correct placement of the chamber load
the items), the set tempera-
ture may be exceeded or it
may take longer until it is
reached.
1 2 3 4 5 6 7 8 9
                     22.4 °C                  10.10.0 %
                                                                                                                                     Manual  Mode
                                                                                                                                      Holz trocknen
                                                                                                                                      aufheizen
                                                                                                          Set      0%                 09:12h
                           Set 37.0 °C             Set        0%
                                                  30%rh
                                                                                                       min       max
                 04  30
                 44 :44
                                                                                                                  max           °C            %rh
                                                                                                     15.0 °C       40.0 °C
                                                                                                                  000°C
                        h        m                                                               min
                                                                                                    auto
                 End   13:30 23.11.
                   End 14:45                             Set 30 %rh
                                                                                                 000°C             auto off
                                                                                                                  ++/-- 0.0
                                                                                                                         99KK        0   12
          ON
          O
          ON
          O NN
10 11 12 13 14 15 16 17 18 19 20
1 2 5 6 7 8 9
                      04 30
                                                                                                       min       max
                 44 :44
                                                                                                                  max           °C            %rh
                                                                                                     15.0 °C       40.0 °C
                                                                                                                  000°C
                       h       m h         m                                                     min
                                                                                                    auto
                  End 14:45 Ende 13:30 23.11.
                                                                                                 000°C            auto off
                                                                                                                  ++/-- 0.0
                                                                                                                         99KK        0   12
          ON
          O
          ON
          O NN
10 11 12 15 16 17 18 19 20
   The status display shows you which operating mode or operating state the appliance is
   currently in. The current operating state is highlighted in colour and indicated by the text
   display:
    Appliance is in programme mode
   ■ Programme is stopped                                       12.Sept.2012      13:44
      Appliance is in manual mode                                  Manual Mode
   The example on the right shows the appliance in manual
   mode, identified by the coloured hand symbol.
                                                                                 3470% rh
Adjustment range: 40 to 97 % rh and OFF
    Humidification is indicated by the    symbol.
    Dehumidification is indicated by the    symbol.
                                                                                     Set      %rh
When the appliance heats up, the humidity is dynamically adjusted
to approach the setpoint depending on the dew point of the
interior temperature.
CO2
                                                                                       CO2
                                                                                      1.15.0 %
Adjustment range: 0 to 20 % in steps of 0.1 %
Set 0%
                                                                                      1.10.0 %
Adjustment range: 1 % to 20 % in steps of 0.1 %
Set 0%
5.5.5 Operation with digital backwards counter with target time setting,
      adjustable from 1 minute to 99 days (timer)
In timer operation, you can adjust the time the appliance runs at the set values. The appliance
has to be in manual operating mode for this.
1. Press the activation key to the left of                                                  TIMER
                                                                                04 30
   hours 30 minutes. The approximate end
   time is shown beneath, in a smaller font.                                            h         m
                                                                                End     13:30 23.11.
The display now shows the remaining time               TIMER               12.Sept.2012             13:44
in a large font and the approximate end time
                                                     04 30
                                                                               Timer active
in a smaller font beneath. The status display
                                                           h          m
shows Timer active.
                                                     End   13:30 23.11.
4. Now, as described under 5.5.2, set the individual values which you want the appliance
     to operate at. The set values can be changed at any time while the timer elapses. The
     changes are effective immediately.
     In Setup, you can choose if the timer should run setpoint-dependent or not. This deter-
     mines whether the timer should not start until a tolerance band around the set tempera-
     ture is reached or if it should start right after activation (see page 51). If the timer runs
     setpoint-dependent, this is indicated by the         symbol in the timer display.
When the timer expires, the display shows 00h:00m.
                                                                                      TIMER
All functions (heating etc.) are switched off. In addition, an
acoustic alarm sounds, which can be turned off by pressing the
confirmation key.                                                                00 00    h        m
   Note that condensation may occur in the interior after you                    End      13:30 23.11.
   switch off the heating.
To deactivate the timer, open the timer display by pressing the
                                                                                   TIMER
activation key again and then turning the turn control to reduce
the timer setting until --:-- is displayed. Confirm with the confirma-
tion key.                                                                        -- -- h             m
5.5.6 Programme mode                                                             End      9:00 23.11.
In this operating mode, programmes saved in the appliance can be started with different
combinations of individual parameters (temperature, humidity, etc.) at staggered intervals,
which the appliance then automatically processes in sequence. These programmes are not cre-
ated directly at the appliance but externally at a computer / laptop and using AtmoCONTROL
software. Transfer to the appliance is possible using the provided USB storage medium or via
Ethernet.
            A description of how to create and save programmes can be found in the separate
            AtmoCONTROL software manual.
         NOTICE
One or several default sterilisation programmes are saved in the appliance (see page 59).
They only serve to sterilise the appliance itself. Do not use them to sterilise medical devices.
The hold time in the sterilisation programme set in the appliance at 180 °C is 1 h. The total
time including heating up and cooling down to 50 °C is 6 h 30 min.
Starting a programme
1. Press the activation key to the right of         Fr 20.10.2010 20:31
                                                    13.Sept.2012  17:44
   the status display. The current operating         Manual mode
                                                     manueller Betrieb
   mode is highlighted automatically, in this          Activate
   example Manual mode ( ).
                                                             APH
                                                           GRAPH
                                                           80
     Only the programme currently selected in menu mode and shown in the display can be
     used. If you want to process another programme, you need to activate it in menu mode
     first (description from page 59).
3. To start the programme, press the confir-                                12.Sept.2012   10:44
   mation key. The programme is executed.
                                                                               Steri 180
   The display shows:
                                                                               Heat up
► the programme description (in this exam-
  ple Steri 180)
► the programme segment description, in
  this example Heat up
► the current run (in case of loops)
     You cannot change any parameters (e.g. the temperature) at the appliance while a
     programme is running. However, the displays ALARM and GRAPH can still be used.
Cancel programme
You can cancel an active programme at any             Fr 20.10.2010 20:31
                                                      12.Sept.2012  10:44
time.
1. Press the activation key to the right of    0%      Steri 180 Betrieb
                                                       manueller
                                                       Heat up
                                                     Emergency operation
          °C
                                                                     Setting MAX
                                                                       Set
                                                                       temperature
Controller error
     °C                   A             B               C              D              E
               52°C                         52°C
 50°C
               48°C                         48°C                           27°C
25°C
                                                                           23°C
                                                                                               t
                      ASF active AUTO              ASF active AUTO                ASF active AUTO
                                    ASF alarm
                                                                                                   30
                                                                                                   75
warning symbol is shown (Fig. 21). If the          EMP
                                                                                                                        LIG
                                                                                                                         IG
                                                                                                                  .0 % %
                                                                                                                         Set 15.0 %
5.6.4 O2 monitoring
(only for appliances in the corresponding configuration)
If O2 monitoring was triggered, this is
indicated by the O2 display: the actual value
is highlighted in red and a warning symbol
    is shown (Fig. 23). If the acoustic alarm                                                O2
                                                                                        30
                                                               FEUCHTE
5.7 Graph
The GRAPH display provides an overview of the chronological sequence of the setpoint values
and actual values for temperature, humidity, CO2 and O2 content as a curve.
Press the activation key to the       °C                 12.09.2012
                                                          Fr 20.10.2010 20:34
                                          0 4
                                       14.00    8 16.00
                                                    12 16 2018.00
                                                               24
                                                                 0 4
                                                              14.00    8 16.00
                                                                           12 16 2018.00
                                                                                      24
4. In the menu mode, prepare the sterilisation programme for execution (see page 59).
5. Switch to the operating mode and start the sterilisation programme as described on page
   31. You cannot set or change any values as long as the programme is running.
6. Once sterilisation is finished and the ap-           Fr 20.10.2010 20:31
                                                        12.Sept.2012  10:44
   pliance has cooled down to 37 °C, end
   the sterilisation programme. To do so,
   press the activation key to the right of
                                                0%          Steri 180 Betrieb
                                                            manueller
                                                            Steri End
0°C
     ASF
            40.4 °C
                  Set 38.5 °C
                                                  customer service                     Page 2
Temperature alarm and           The adjustable    Increase the difference between      Page 35
"TWW" are displayed             temperature       the monitoring and setpoint
                                controller        temperature – by either increas-
           TEMP                 (TWW) has as-     ing the max value of the tem-
            42.4 °C
                                sumed heating     perature monitoring or decreas-
                                control.          ing the setpoint temperature.
     TWW          Set 38.5 °C                     If the alarm continues: Contact
                                                  customer service                     Page 2
Temperature alarm and           The mechanical    Switch off the appliance and
"TB" are displayed              temperature       leave to cool down.
                                limiter (TB)      If the error occurs again, contact   Page 2
           TEMP                 permanently       customer service.
            72.4 °C
                                switched off
                                heating.
     TB           Set 38.5 °C
        55.4 %rh
              Set 55 .0 %rh
Alarm display (MaxAl)         Upper hu-      Open the door for 30 sec. and
                              midity limit   wait to see if the appliance reli-
        HUMIDITY              exceeded       ably adjusts to the setpoint.
        75.4 %rh
                                             If the error occurs again, contact     Page 2
                                             customer service.
      MaxAl Set 70 .0 %rh
        55.4 %rh
                                             trays. If required, refill the water
                                             tank / water trays.
      MinAl Set 60 .0 %rh                    If the error occurs again, contact     Page 2
                                             customer service.
6.1.3 CO2 Monitoring
6.1.4 O2 monitoring
Alarm indicates that the                        Open the door for 30 sec. and
lower O2 alarm limit was                        wait to see if the appliance then
undercut                                        steadily adjusts to the setpoint.
                                                If the error occurs again, contact
           O2                                   customer service.                    Page 2
          37.4 °C
                                                               service as soon as
                                                               possible
         T:E-3 Set 37.0 °C
          37.4 °C
                                                               service as soon as
                                                               possible
        AI E-3 Set 37.0 °C
       E-3 °C
                                                             ► Contact customer Page 2
                                                               service
               Set 45.0 °C
7.         Menu mode
In menu mode, you can make basic settings, load programmes and export protocols, as well
as calibrate the appliance.
NOTICE
► Before changing menu settings, read the description of the respective functions on the fol-
     lowing pages to avoid possible damage to the appliance and/or chamber load.
To enter menu mode, press the MENU key.
    To exit the menu mode at any time, press the MENU key
    again. The appliance then returns to operating mode. Only
    changes that are accepted by pressing the confirmation key
    are saved.
7.1 Overview
Press the MENU key to change between the displays in menu mode:
1 2 3 4 5 12 13 14 15
                LANGUAGE             TIME
                               SIGNALTÖNE                                         PROG          PROTOCOL
     ON
     O N
     6           7         8                9   10 11              16      17              18               19
Fig. 26     ControlCOCKPIT in menu mode
1    Language selection activation key                10 Turn control for adjustment
2    Language selection display                       11 Confirmation key (accepts setting made
3    Date and time display                               with the turn control)
4    Date and time setting activation key             12 Programme selection activation key
5    Exit menu mode and return to operating           13 Programme selection display
     mode                                             14 Protocol display
6    Setup activation key (basic appliance set-       15 Protocol activation key
     tings)                                           16 Acoustic signal adjustment activation key
7    Setup display (basic appliance settings)         17 Acoustic signal adjustment display
8    Adjustment display                               18 USER ID display
9    Adjustment activation key                        19 USER ID display activation key
All other settings can be made accordingly. The settings possible are described in the follow-
ing sections.
    If no new values are entered or confirmed for approx. 30 seconds, the appliance automati-
    cally restores the former values.
7.3 Setup
7.3.1 Overview
In the SETUP display, you can set the following parameters:
► the IP address and subnet mask of the appliance's Ethernet interface (for connection to a
    network)
► the temperature display unit (°C or °F, see page 50)
► how the digital backwards counter with target time setting works (Timer Mode, see page
    51)
► Remote control (see page 51)
► Gateway (see page 51)
    If the Setup menu contains more entries than can be                                 Setup
    displayed, this is indicated by the display "1/2". This
    means that there is a second "page" of entries.           1/2
To display the hidden entries, use the turn control to scroll IP adress    255. 145. 1 3 6 . 225
beyond the lowest entry. The page display changes to "2/2".   Subnet mask  255. 255. 0 . 0
                                                                                                                                                                                                                Unit                         °C                          F
7.3.2 IP address and subnet mask                                                                                                                                                                                Al                       T
If you want to operate one ore more appliances in a network, each appliance must have its
own unique IP address for identification. By default, each appliance is delivered with the IP
address 192.168.100.100.
                              AtmoCONTROL   Gerät   Programm   Protokoll   Drucken   Einstellungen   Hilfe
                                  i
                                                                                           15
                       37.0°C
                      44.4 %rh    i
- +
                                                                                                                                                                         192.168.1
                                                                                                                                                                         192.168.1.216
                                                                                                                                                                                         LAN 1: 192.168.1.233
LAN 2: 192.168.1.215
LAN 3: 192.168.1.241
                                                                                                   SETUP
2. Accept the selection by pressing the con-
   firmation key. The first three digits of the                IP address         192. 168. 1 0 0 . 100
   IP address are automatically selected.                      Subnet mask        255. 255. 0 . 0
                                                               Unit                  °C             F
                                                               Alarm temp            TWW            TWB
                                                               Timer mode
                                                                                                   SETUP
3. With the turn control, set the new num-
   ber, e.g. 255.                                              IP address         255. 168. 1 0 0 . 100
                                                               Subnet mask        255. 255. 0 . 0
                                                               Unit                  °C             F
                                                               Alarm temp            TWW            TWB
                                                               Timer mode
                                                                                                   SETUP
4. Accept the selection by pressing the
   confirmation key. The next three digits of                  IP address         255. 168. 1 0 0 . 100
   the IP address are automatically selected.                  Subnet mask        255. 255. 0 . 0
   Setting these is done according to the                      Unit                  °C             F
                                                               Alarm temp            TWW            TWB
   description above.
                                                               Timer mode
                                                                                                   SETUP
5. After setting the last three digits, accept
   the new IP address by pressing the con-                     IP address         255. 145. 1 3 6 . 225
   firmation key. The selection returns to the                 Subnet mask        255. 255. 0 . 0
   overview.                                                   Unit                  °C             F
   The subnet mask is set accordingly.                         Alarm temp            TWW            TWB
                                                               Timer mode
Unit
                                                                                                     SETUP
Here, you can choose whether the temperature is displayed in
°C or °F.                                                       IP address         255. 145. 1 3 6 . 225
                                                                Subnet mask        255. 255. 0 . 0
                                                                Unit                   °C             °F
                                                                Alarm temp             TWW            TWB
                                                                Timer mode
         A                       tTimer
                  B                           tTimer
  6K
                                                                                                    t
Fig. 28     Timer mode
A     Timer independent of setpoint: Timer starts right after activation
B     Timer setpoint-dependent: Timer does not start until tolerance band is reached
►    Read only
►    Write
►    Write + Alarm
If the appliance is in remote control mode, the      symbol
appears in the temperature display. In the settings Write
and Write + Alarm, the appliance cannot be controlled at                        TEMP
                                                                                 23.2 °C
the ControlCOCKPIT unless the remote control is switched
off (setting Off) or set to Read only.
     In order to use the remote control function, program-
     ming skills and special libraries are required.
                                                                                         Set 38.0 °C
7.3.5 Gateway
The setup entry gateway is used to connect two networks
with different protocols.
The gateway is set the same way as the IP address (see page            Remote Control Off
49).                                                                   Gateway        192.168.5 .1
NOTICE
Always set the time zone (and daylight saving time yes/no) before you set the date and time.
Avoid changing the set time after that since this can lead to gaps or overlapping when record-
ing measured values. If you still need to change the time, you should not run a programme
immediately before or after doing so.
   Daylight saving time and standard time are not changed automatically. For this reason,
   please keep in mind to adjust them at the beginning of each period.
8. Now, set date (day, month year) and time
   (hours, minutes) in the same way. Accept                           Date             27 . 05 . 2012
   the selection by pressing the confirma-                            Time             12 : 00
   tion key.                                                          Time zone        GMT 00:00
                                                                      Daylight savings
7.5 Calibration
           NOTICE
To guarantee perfect control, we recommend to calibrate the appliance once a year. The cali-
bration possibilities depend on the appliance configuration.
7.5.1 Temperature calibration
The appliances are temperature calibrated and adjusted at the factory. In case readjustment
should be necessary later on – for example due to influence of the chamber load – the ap-
pliance can be calibrated customer-specifically using three calibration temperatures of your
choice:
► Cal1       Temperature calibration at low temperature
► Cal2       Temperature calibration at medium temperature
► Cal3       Temperature calibration at high temperature
   For temperature calibration, you will need a calibrated reference measuring device.
                                                                        CAL 3
                                                                        +0,4°C
                                                             CAL 2
                                                  CAL 1      -0,4°C
                                                  +0,5°C
                                           tion
                                    calibra
                                ory
                            fact
                                                                   42.0°C
   erence instrument should display 43.6 °C.
                                                                                                   43.6 °C
                                                                            Set 42.0 °C
                                                             42.0°C
    temperature measured by the reference
    instrument should now also be 42 °C.
                                                                  Set 42.0 °C
                                                                                    42,0 °C
With Cal1, a calibration temperature below Cal2 can be programmed accordingly, and with
Cal3, a temperature above. The minimum interval between the Cal values is 10 K.
If all calibration values are set to 0.0 K, the factory calibration settings are restored.
For temperature calibration, you will need a calibrated reference measuring device.
                                                                                 rh 95
                                                                                +1,5 %
                                                                   rh 85
                                                                  -1,5 %
                   rh 50
                  +3,0 %
                                         on
                                calibrati
                             ry
                        Facto
                                                                                                              JUSTIEREN
5. Save the setting by pressing the confir-
   mation key. The corresponding calibra-                                                Temperature Cal1 40.0 %rh - 0,5 %
   tion value is automatically highlighted.                                              Humidity    Cal2 60.0 %rh +1,0 %
                                                                                         CO2         Cal3 80.0 %rh +1,0 %
                                                                                         O2
                                                                                                        29.5 %rh
   chamber of the appliance.
8. Close the door and, in manual mode,
   adjust the set humidity to 60 % rh.                                                                        Set 60.0 %rh
                                                        60.0 %rh
   reference instrument should display
   58.5 % rh.
                                                                                   58.5 %rh
                                                              Set 60.0 %rh
    ting by pressing the confirmation key.                             CO2         Cal3 80.0 %rh +1,0 %
                                                                       O2
                                                        60.0 %rh
    ment should now also be 60 % rh.
                                                                                   60.0 %rh
                                                              Set 60.0 %rh
                                           Cal2              Cal3
                                          +1,5 %            -0,7 %
                                                       on
                         Cal1                   librati
                                         tory ca
                        -0,4 %
                                      Fac
0% 10% 20%
                                                                                                                JUSTIEREN
5. Save the setting by pressing the confir-
   mation key. The corresponding calibra-                                                Temperature Cal1 5.0 %CO2 - 0,5 %
   tion value is automatically highlighted.                                              Humidity    Cal2 10.0 %CO2 +1,0 %
                                                                                         CO2         Cal3 15.0 %CO2 +1,0 %
                                                                                         O2
                                                                                                          1.10.0 %
   chamber of the appliance.
8. Close the door and, in manual operating
   mode, adjust the CO2 content setpoint to                                                               Set       0%
   10 %.
    ting by pressing the confirmation key.                            CO2         Cal3 15.0 %CO2 +1,0 %
                                                                      O2
7.6 Programme
In the Program display, programmes created using the AtmoCONTROL software can be
transferred to the appliance and saved on a USB storage medium. Here, you can also select
the programme provided for use (see page 31) and delete programmes.
         NOTICE
One or several default sterilisation programmes are saved in the appliance. They only serve to
sterilise the appliance itself and must not be used to sterilise medical devices.
If you connected a USB storage medium, you can now remove it.
To delete a programme, select Delete with the turn control and select the programme to be
deleted the same way you can select a programme for activation. Sterilisation programmes
cannot be deleted.
7.7 Sounds
In the SOUND display, you can define whether or not the appliance should emit acoustic
signals and, if yes, define for which events it should do so:
► on the press of a key
► at the end of a programme
► On alarm
► if the door is open
1. Activate the acoustic signal adjustment.                                                         Sound
7.8 Protocol
The appliance continually logs all relevant measured values, settings and error messages at
1-minute intervals. The internal log memory is of the continuous memory type. The logging
function cannot be switched off and is always active. The measured data are stored in the
appliance, safe from manipulation. If the power supply is interrupted, the time of the power
failure and voltage recovery are stored in the appliance.
You can export the protocol data for different periods to a USB storage medium via the USB
interface or, via Ethernet, import them to the AtmoCONTROL software for graphical represen-
tation, print-out or storage.
     The log memory of the appliance is not modified or deleted by reading it out.
1. Connect the USB storage medium to the
   interface on the right side of the Control-
   COCKPIT.
7.9 USER ID
7.9.1 Description
With the USER ID function, you can lock the settings of individual (e.g. temperature) or all
parameters, so that they cannot be changed at the appliance by accident or unauthorised
persons. You can also lock setting options in menu mode (e.g. adjustment or date and time
settings) this way.
    If adjustment options are locked, this is indicated
                                                                    TEMP
                                                                          22.4 °C
    by the lock symbol in the respective display (Fig.
    32).
USER ID data is entered in the AtmoCONTROL software
and saved on the USB storage medium. The USB stor-
age medium is thus acting as a key: Parameters can                          Set 40 4 °C
only be locked or unlocked if it is connected.            Fig. 32     Temperature adjustment
                                                          at appliance locked (example)
           A description of how to create a USER ID in
           AtmoCONTROL is provided in the separate AtmoCONTROL manual.
           Danger due to electric shock. Disconnect the mains plug before any
           cleaning or maintenance work.
9.2 Disposal
This product is subject to the Directive 2002/96/EC on Waste Electrical
and Electronic Equipment (WEEE) of the European Parliament and of
the Council. This appliance has been brought to market after August
13th, 2005 in countries which have already integrated this directive
into their national laws. It may not be disposed of in normal household
waste. For disposal, please contact your dealer or the manufacturer.
Any appliances that are infected, infectious or contaminated with
materials hazardous to health are excluded from return. Please also
observe all other regulations applicable in this context.
Before disposing of the appliance, please render the door locking
mechanism unusable, for example to prevent playing children from be-
ing locked inside the appliance.
There is a lithium battery in the ControlCOCKPIT of the appliance. Re-
move it and dispose of it in accordance with the regulations in your country (Fig. 33).
Index
A                              Disposal 65                    Log memory 46, 61
Accessories 16                 Door 25
                                                              M
Accidents 9                    E                              Maintenance 64
Activation button 28           Electrical connection 12       Malfunctions 9, 42, 44
Alarm 33, 34, 36, 38, 39, 42   Electrical equipment 12        Manufacturer 2
Ambient conditions 16          Electronic temperature moni-   Material 12
Ambient temperature 16             toring 33                  Mechanical temperature
Anlieferung 68                 Emergency 10                      monitoring 35
Appliance error 44             Ending operation 41            Menu 47
ASF 33, 34                     End of programme 32            Menu mode 47
AtmoCONTROL 3, 13, 16, 28,     Error message 45, 46           Minimum clearances 19
    31, 59, 61, 62             Error messages 42              Monitoring function 33
B                              Ethernet 13                    Monitoring temperature 33
Basic device settings 47       Explosion protection 8
                                                              N
Basic settings 47              F                              Nameplate 13
C                              Fan speed 30                   Network 13, 49
Calibration 53                 Forklift truck 18
                                                              O
Cancel programme 32            Fresh water container 22
                                                              O monitoring 39
Carrying 17                    G                              Operating 24
Chamber load 26                Gas bottles 6, 7, 8, 9, 21,    Operating modes 28
Changes 9                         23, 24                      Operating personnel 8
Cleaning 64                    Gas connection 22              Operating problems 44
CO2 30                         Gas leakage 9                  Operation 24
CO2 connection 22              Gateway 51
CO and O calibration 57                                       P
                               Graph 38, 39
Cold burns 7                                                  Packaging material 18
Communication interfaces       H                              Parameter adjustment 28,
    12                         Hazards 6                          48
CO monitoring 38               Hot-air generator 11           Power failure 46
Compensation correction        Humidity 29                    Power supply 21, 61
    values 55                  Humidity adjustment 55         Product safety 6
Connections 12                 Humidity monitoring 36, 37,    Programme 59
ControlCOCKPIT 26, 27             38, 39, 44                  Programme mode 28, 31
Customer service 2             Humidity profile 40            Protocol 61
D                                                             Pt100 temperature sensor
                               I
                                                                  33
Danger of suffocation 7,       Inbetriebnahme 68              Putting into operation 21
    23, 24                     Installation site 19
Date 52                        Intended use 8                 R
Declaration of conformity 15   Interfaces 12                  Regular maintenance 64
Decommissioning 65             IP address 49                  Remote control 51
Delete programme 60
Delivery 17, 18, 21            K                              S
Digital backwards counter      Keysound 60                    Safety regulations 6, 11
    with target time setting                                  Service 64
                               L
    30                                                        Servicing 64
Dimensions 15                  Language selection 48          Setting up 17, 19
Directives 15                  Loading the appliance 26       Setup 49
Appendix
     Technical description according to EN 60601-1-2
EMC – Guidance
                                             ICO50med
                                            ICO105med
                                            ICO150med
                                            ICO240med
68
                                                                                                          Appendix
The Memmert CO2 Incubator ICOxxmed is intended for use in the electromagnetic environment specified
below. The customer or user of the CO2 Incubator ICOxxmed should assure that it is used in such an
environment.
      Immunity            IEC 60601             Compliance           Electromagnetic environment –
         test              test level               level                         guidance
Electrostatic       ± 6 kV contact         ± 6 kV contact      Floors should be wood, concrete or
discharge (ESD)                                                ceramic tile. If floors are covered with
                    ± 8 kV air             ± 8 kV air          synthetic material, the relative humidity
IEC 61000-4-2                                                  should be at least 30 %.
Electrical fast     ± 2 kV for power       ± 2 kV for power    Mains power quality should be that of a
tranient / burst    supply lines           supply lines        typical commercial or hospital
                                                               enviroment.
IEC 61000-4-4       ± 1 kV for input /     ± 1 kV for input /
                    output lines           output lines
Surge               ± 1 kV differential    ± 1 kV differential Mains power quality should be that of a
                    mode                   mode                typical commercial or hospital
IEC 61000-4-5                                                  enviroment.
                    ± 2 kV common          ± 2 kV common
                    mode                   mode
Voltage dips, short < 5 % UT               < 5 % UT            Mains power quality should be that of a
interruptions and   (>95 % dip in UT)      (>95 % dip in UT)   typical commercial or hospital
voltage variations  for 0,5 cycle          for 0,5 cycle       enviroment.
on power supply
input lines         40 % UT                40 % UT             If the user of the CO2 Incubator
                    (60 % dip in UT)       (60 % dip in UT)    ICOxxmed requires continued operation
IEC 61000-4-11      for 5 cycles           for 5 cycles        during power mains interruptions, it is
                                                               recommended that the CO2 Incubator
                    70 % UT                70 % UT             ICOxxmed will be powered from an
                    (30 % dip in UT)       (30 % dip in UT)    uninterruptible power supply.
                    for 25 cycles          for 25 cycles
                       < 5 % UT               < 5 % UT
                       (>95 % dip in UT)      (>95 % dip in UT)
                       for 5 s                for 5 s
Power frequency        3 A/m                  Not applicable
(50/60 Hz)
magnetic field
IEC 61000-4-8
NOTE         UT is the mains voltage prior to application of the test level.
                                                                                                                69
Appendix
      The Memmert CO2 Incubator ICOxxmed is intended for use in the electromagnetic environment specified
      below. The customer or user of the CO2 Incubator ICOxxmed should assure that it is used in such an
      environment.
           Immunity            IEC 60601           Compliance           Electromagnetic environment –
              test              test level             level                        guidance
                                                                  Portable and mobile RF communications
                                                                  equipment should be used no closer to any
                                                                  part of the CO2 Incubator ICOxxmed,
                                                                  including cables, than the recommended
                                                                  seperation distance calculated from the
                                                                  equation applicable to the frequency of
                                                                  the transmitter.
NOTE 1 At 80 MHz and 800 MHz, the higher frequency range applies.
      NOTE 2 These guidelines may not apply in all situations. Electromagnetic propagation is affected by
      absorption and reflection from structures, objects and poeple.
       a
         Field strengths from fixed transmitters, such as base stations for radio (cellular/cordlass) telephones
      and land mobile radios, amateur radio, AM and FM radio broadcast and TV broardcast cannot be
      predicted theoretically with accuracy. To assess the electromagnetic enviroment due to fixed RF
      transmitters, an electromagnetic site survay should be considered. If the measured field strength in the
      location in which the CO2 Incubator ICOxxmed is used exceeds the applicable RF compliance level above,
      the CO2 Incubator ICOxxmed should be observed to verify normal operation. If abnormal performance is
      observed, addidional measures may be necessary, such as re-orienting or relocating the CO2 Incubator
      ICOxxmed.
       b
           Over the frequency range 150 kHz to 80 MHz, field strengths should be less than 3 V/m.
70
                                                                                                         Appendix
NOTE 1 At 80 MHz and 800 MHz, the separation distance for the higher frequency range applies.
 NOTE 2 These guidelines may not apply in all situations. Electromagnetic propagation is affected by
 absorption and reflection from structures, objects and poeple.
                                                                                                               71
   CO2 Incubator ICOmed