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CELL PORCESSES
OBJECTIVES
- identify different parts of plant and animal cells.
- identify from fresh preparations and diagrams or on photomicrographs cell membrane, cytoplasm, cell
organelles: nucleus, mitochondrion and ribosome of an animal cell.
- identify from fresh preparations and diagrams or on photomicrographs the cell wall, cell membrane, sap
vacuole, cytoplasm, nucleus and chloroplasts of a plant cell.
- state the functions of the nucleus.
- state the functions mitochondrion, ribosome, cell wall and cell membrane.
- describe the relationship between cell structure and function using a red blood cell and nerve cell in animals and
root hair cell and xylem in plant cells.
- define tissue, organ, system and organism.
- define diffusion
- define osmosis
- describe the effect of concentration gradient in the uptake of water by plants.
- describe the effect of osmosis on plant tissues, in terms of flaccid cells, turgid cells and plasmolysis.
- describe the effect of osmosis on animal tissues. [ refer to bursting and shrinking ].
- conduct experiments using solutions of varying concentration to demonstrate the process of osmosis
NOTES
What is a cell?
It is the basic unit of life
Types of cells
There are two types
      Animal
      Plant
Structure of animal cell
Draw and label
Structure of plant cell
Draw and label
STRUCTURE                              PROPERTIES                              FUNCTIONS
Cell membrane                          2 lipid layer with one                  Selects substances entering
                                       protein layer sandwiched in             & leaving the cell
                                       between
Nucleus                                Contains genetic material               Regulates cellular activities
                                       (chromosomes)
Cytoplasm                              Contains water & dissolved              Where biochemical reaction
                                       substances e.g. glucose,                take place
                                       minerals, vitamins
Ribosome                               Small grains found                      Responsible for protein
                                       scattered in the cytoplasm,             formation
                                       some may be attached to
                                       endoplasmic reticulum
mitochondrion                          Surrounded by 2                         Site for aerobic respiration
                                       membranes, inner
                                       membrane has folds
Cell wall                              Made carbohydrate called                Protects cell against
                                                                                                     2
                                   cellulose, its freely              mechanical damage & gives
                                   permeable                          plant a permanent shape
   vacuole                         Has concentrated solution          Stores the cell sap (vital
                                   made of salts, glucose             chemicals for the cell)
   SPECIALIZED CELLS
   These are cells which have stopped growing, have a definite shape and perform one specific
   function.
   Examples of specialized cells.
Cell                   Function                          Relationship between structure & Function.
1. Red Blood Cells     Responsible for transporting      - Has a biconcave structure that helps it move in
(Erythrocytes)         O2                                the blood vessels
                                                         - Has a spongy cytoplasm that helps it to
                                                         compress itself in blood vessels with small lumen
                                                         - Absence of nucleus increases surface area for
                                                         transportation of O2
                                                         - Presence of haemoglobin that binds to O2 and
                                                         carry it as an unstable compound called
                                                         oxyhaemoglobin
2. Muscle cell                                           - Long & have numerous protein fibres in the
                       It moves bones in different       cytoplasm. (fibre helps to shorten the cell when
                       directions (contract / relax to   there is energy)
                       help move structures)              - Can of contract & relax in order to move parts
3. Sperm cell                                            - Has streamlined head to enable it to move
                       Fuse with ovum to from a          towards ovum with less friction
                       zygote                            - Has a tail to help it swim towards the ovum.
4. Ovum                                                  - It has a large food store to provide the zygote
                       Fuse with sperm to from a         with nourishment before implantation
                       zygote
5. White Blood Cells   Phagocytes – engulf & digest      - Phagocytes are polymorphs; can change shape
(Leucocytes are two    bacteria (pathogens)              of cytoplasm like amoeba.
types; Phagocytes &                                      - Have Granular cytoplasm with lobed nucleus.
Lymphocytes)           Lymphocytes – produce
                       chemicals called antibodies
                       that kill bacteria
6. Root hair cell      - Absorb water from the soil      - Large number of mitochondria to generate
                       - Absorb mineral ions (salts)     energy needed fro active transport of ions
                       from the soil                     - Has a finger-like projection that enables to
                       - anchor the plants firmly to     penetrate tightly packed soil particles
                       the ground.                       - Have Large concentrated sap vacuole which
                                                         ensures water uptake through osmosis
7. Xylem cells         - Transport water & dissolved     - Has dead hollow tube without cytoplasm to allow
                                                                                                        3
                        minerals from the roots to top    flow of water
                        part of a plant                   - Walls are lignified (coated with lignin) to
                        - Supports the plant against      withstand water pressure
                        mechanical damage                 - cylindrical cells are attached end to end form a
                                                          xylem vessel.
8. Nerve Cell           Transmits nerve impulses          - Have very long fibres which connect distant
(neuron)                (messages) around the body        parts of the body
                                                          - Fibres are coated with sheath to prevent loss &
                                                          distortion of messages.
                                                          - Chemical reactions cause the impulses to travel
                                                          along the fibre
9. Palisade cell        To make sugar (starch)            - Has lots of chloroplasts to produce chlorophyll
                        through photosynthesis            which traps sunlight energy for the process
                                                          - Elongated so that they can pack tightly
                                                          - Located close to the upper epidermis to receive
                                                          more sunlight
10. Guard cells         To regulate the opening &         - Crescent shaped
                        closing of stomata                - Always paired to form a stomata
                                                          - Are able to change shape to close / open
                                                          depending on whether its day or night.
  Cell Organization
  Cells are organized into tissues, organs, systems and ultimately an organism (plant / animal)
      A Cell; is the basic unit of life
      A Tissue; is a group of similar cells carrying out the same function. E.g. xylems cells make
       xylem tissue
      An Organ; is a group of tissues which work together to perform a particular function. E.g. the
       heart is organ made of nerve tissue, muscle tissue, epithelial tissue etc.
      A System; is a group of organs which work together to perform a particular function. E.g. the
       digestive system is made of; stomach, mouth, intestine, liver etc
      An Organism; Is a group of different systems which come together to form a living thing e.g.
       Human beings are made of; nervous, skeletal, circulatory, digestive, muscle systems etc.
  Movement of Substances in & out of cells
  There are three processes that are involved in the movement of substances in & out cells
      Diffusion
      Osmosis
      Active transport
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1. Diffusion;
It is the random movement of particles from their region of their higher concentration to their
region low concentration down a concentration gradient until they are evenly distributed.
N.b. Diffusion occurs in all states of matter.
a) Diffusion in gases
The spread of perfume particles (or air freshener) from the nozzle of the spray can to fill up the
room occurs through diffusion.
b) Diffusion in liquids
The spread of potassium permanganate from the bottom of a beaker to the top is by diffusion.
Diffusion is affected by;
      I. Changes in temperature;
        An increase in tesmperature increases the kinetic energy of particles, hence particles will
        move faster leading to an increase in the rate of diffusion
     II. Particle size
       Small particles dissolve faster because they are less dense & have a large surface area
       hence can diffuse rapidly from one end to the other.
    III. Concentration gradient
        This is the difference in the amount of particles between two adjacent areas. Particles
        move faster when the difference in the amount of particles is large. The rate of diffusion
        diminishes as the concentration gradient reduces.
2. Osmosis;
It is the random movement of water molecules from their region of higher concentration to their
region of lower concentration along the concentration gradient through a partially / selectively
permeable membrane.
Similarities between Osmosis & Diffusion
           Particles are moving down the concentration gradient
           Both processes are passive, no energy is expended (used)
Differences between Osmosis & Diffusion
                   OSMOSIS                                             DIFFUSION
Occurs mainly in liquids (only water molecules      Occurs in all states of matter (any particle is
are moved)                                          moved)
Materials are moved across a barrier (a             Does not require a barrier (partially membrane)
partially permeable membrane required)
3. Active Transport
This is the movement of materials from their region of low concentration to their region of high
concentration against the concentration gradient using energy from respiration. Substances are
moved through the aid of protein carrier molecules and a barrier is not important.
Active transport is different from the other two modes of movement in that;
     Unlike osmosis & diffusion, it occurs against the concentration gradient
     It requires energy
     Only mineral ions are moved
Osmosis and cells;
A cell can be exposed to the following conditions;
     Immersed in pure (distilled) water / dilute solution
     Immersed in a concentrated solution
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??? Explain what happens when a plant cell is immersed in pure water / dilute solution
Ans; There will be an influx of water from the container into the cell by osmosis leading to an
increase in the contents of the protoplasm. As a result turgor pressure builds inside the cell
membrane causing it to stretch until it touches the cell wall. When this happens the cell is said to
be Turgid.
??? Explain what happens when a plant cell is immersed in a concentrated solution.
Ans; the water molecules will move out of the cell by osmosis. The outflux of water molecules will
lead to a decrease in the contents of the protoplasm hence a decrease in turgor pressure. The
cell membrane will become flaccid and pull away from the cell wall. When this happens the cell is
said to be plasmolysed.
??? Explain what happens when an animal cell is immersed in pure water / dilute solution
Ans; Water molecules will move into the cell by osmosis and due to build up of turgor pressure
the cell membrane will burst (since the animal cell has no cell wall to limit the stretching of the
membrane).
??? Explain what happens when a animal cell is immersed in a concentrated solution.
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    Ans; Due to outflux of water by osmosis, the cell will decrease in size and when this happens the
    cell is said to be shrunken.
ACTIVITY; The Effect of various sugar concentrations on potato tissue
Apparatus & materials
 5 different solns; 0%, 5%, 15%, 25% and 60%
 5 petri dishes
 Cork borer
 White tile
 Scalpel
 30cm ruler
 Large potato
 25ml measuring cylinder
Procedure
- Label the petri dishes A to E.
- Place equal volumes (10ml) of the sugar concentrations in the petri dishes as; 0% in petri dish A
5% in dish B, 15% in dish C etc.
- Prepare 5 equal sized e.g. (40mm) potato slices by boring the potato using a cork borer
- Place one potato slice in each petri dish.
- Leave the potato slices in the solutions for 30 – 60 minutes. The remove the slices & blot them with
a paper tissue.
- Measure the final lengths of the slices and record the results as follows
        Sugar soln      Initial length    Final length       Change in length
            0%
            5%
           15%
           25%
           60%
    Practice Questions on the activity above;
        Plot a graph of sugar concentration against change in length
        From the graph, work out the concentration of the potato tubes
        At what concentration would the concentration of the sap vacuole of the potato equal that
           of the surrounding sugar soln?
        What process is responsible for the change in length of the potato tubes?
        What is the formula for calculating the change in length of the potato tubes?
        What else can be measured to find out the effect of concentration on the potato slices?