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553/2
BIOLOGY
PRACTICAL
Paper 2
15th/June/2023
2 hours
UGANDA CERTIFICATE OF EDUCATION
BIOLOGY PRACTICAL
Paper 2
2 HOURS
INSTRUCTIONS TO CANDIDATES:
Answer all questions.
Drawings should be made in the spaces provided.
Use sharp pencils for your drawings.
No additional sheets of writing paper should be inserted.
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1. You are provided with solutions A and B.
Carryout the following tests to establish the nutrient composition of solution A.
Record your observations and deductions in table 1. (9 marks)
Table 1
Tests Observation Deductions
(i) To 1cm3 of solution A
in a test tube, add 1cm3
of benedict’s solution
and boil.
(ii) To 1cm3 of solution a
in a test tube, add 3
drops of iodine
solution.
(iii) To 1cm3 of solution A
in a test tube, add 1cm3
of dilute sodium
hydroxide solution and
3 drops of Copper
sulphate solution.
(b) Label 2 test tubes as 1 and 2.
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Add contents to each test tube as shown in table 2.
Table 2:
Test tube Contents
1 2cm3 of A + 1cm3 of B + 1cm3 of sodium hydroxide solution.
2 2cm3 of A + 1cm3 of B + 1cm3 of Hydrochloric acid.
Shake the contents well and incubate the test tubes for 30 minutes in a water bath
maintained at 37oC – 40oC. Shake the contents periodically.
After 30 minutes, remove the test tubes from the water bath and carry out an iodine test
on the contents of each test tube.
Record your observations and deductions in table 3. (6 marks)
Table 3:
Test Test tube Observations Deductions
To 1cm3 of the 1
contents of the test
tube, add 3 drops of
iodine solution.
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(c) Explain your results for the test tubes in table 3 above.
Test tube 1
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Test tube 2
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(d) State what is being investigated in this experiment? (1 mark)
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2. You are provided with specimens R and S which are flowers.
(a) Give four reasons why they are flowers. (2 marks)
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(b) Give a reason in each case, state the type of pollination of each specimen.
(4 marks)
Specimen Type of pollination Reason
R ……..………….……….. …………….….………….…….…..
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S ……..………….……….. …………….….………….…….…..
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(c) Give three structural differences between specimen R and S. (3 marks)
Specimen R Specimen S
(i)
(ii)
(iii)
(d) Observe specimens R and S.
Describe;
(i) the pistil of specimen R. (2 marks)
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(ii) the androecium of specimen S. (2 marks)
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(e) Give two ecological advantages of specimen R over S. (2 marks)
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(f) Remove all the floral parts of specimen R to expose the pistil.
Draw and label the exposed pistil. State your magnification. (5 marks)
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3. You are provided with specimens J and K. Use the specimens and answer the questions that
follow.
a) Examine the specimens and state three features that can be used to place the specimens in the
same phylum. (3marks)
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b) Describe four observable characteristic features common to both specimens J and K (4marks)
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c) Explain four ways in which specimen J is adapted for survival in its habitat (4 marks)
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d) With the help of a hand lens. Examine the structures on the abdomen of each of the specimens
J and K. State three structural differences between the two. (3 marks)
Specimen J Specimen K
e) With the help of a hand lens where necessary, observe one of the hind limbs of specimen J.
Draw and label the first four segments of the limb. State the magnification of your drawing
(6 marks)
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CONFIDENTIAL INFORMATION
Each student should be provided with the following.
Hibiscus flower, labeled R.
Sodom apple / Solanum incanum flower, labeled S.
Morning glory flower, labelled T.
Crotalaria flower, labelled U.
A cockroach, labeled J.
A worker bee, labeled K.
10cm3 of 1% starch solution labeled A.
5cm3 of 2% amylase labeled B.
2M Hydrochloric acid.
1M sodium hydroxide solution.
Plastic cup.
Thermometer
Hot water
Reagents for carrying out food tests.
