QP Code: 721006 Reg. No……………………….
Seventh Semester B. Pharm Degree Supplementary Examinations
May 2024
Instrumental Methods of Analysis
(2017 Scheme)
Time: 3 Hours Max. Marks: 75
Answer all questions to the point neatly and legibly • Do not leave any blank pages between
answers • Indicate the question number correctly for the answer in the margin space
Answer all parts of a single question together • Leave sufficient space between answers
Draw diagrams wherever necessary
Essays (2x10=20)
1. Explain the construction and working of a double beam UV – Visible
Spectrophotometer
2. Explain various types of interferences in atomic absorption spectroscopy and
methods to reduce the same
Short Notes (7x5=35)
3. Beer-Lambert’s equation
4. What is a grating. Explain its construction and working
5. Explain the construction and working of Nichrome Wire and Nerst Glower
6. List any five applications of turbidomerty
7. Differentiate between Thin Layer Chromatography and High Performance Thin
Layer Chromatography
8. Explain derivatisation in Gas Chromatography with suitable examples
9. Explain the principle of ion exchange chromatography in detail
Answer Briefly (10x2=20)
10. Define chromophore with an example
11. Name the types of wavelength selectors used in UV _Visible spectrophotometer
12. What is inner filter effect in fluorescence quenching
13. Why Infra-Red Spectrum generally has an inverted peak
14. Name any two radiation sources used in Atomic Absorption Spectrophotometer
15. Explain intersystem crossing in flurimetry
16. Name the different types of vibrational modes in infrared spectroscopy
17. Explain electrophoretic mobility and factors affecting it
18. List any two commonly used column dimensions of Gas Chromatography
19. List pharmaceutical applications of gel filtration
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