Name:
UV/Vis Spectrophotometry
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1. Material of cuvette used with visible light 2. A measure of the amount of light absorbed by a
4. The gradient of the line in a graph of absorbance sample.
( ) versus concentration ( ) 3. A small, transparent container that holds the
5. A region of the electromagnetic spectrum with sample and has a fixed path length, typically 1 cm.
wavelengths ranging from 400 nm to 700 nm. 4. A technique used to measure how much light is
7. A measure of the energy of light, usually absorbed or transmitted by a substance as a
expressed in nanometers function of wavelength
8. The distance the light travels through the sample, 6. A linear relationship that relates absorbance to
usually measured in centimeters (cm). the concentration ( ), path length ( ), and molar
9. Material of cuvette used with ultraviolet light absorptivity ( )
11. A reference sample that contains all 10. A region of the electromagnetic spectrum with
components of the solution except the analyte of wavelengths ranging from 10 nm to 400 nm.
interest. 14. The component of the spectrophotometer that
12. The amount of a substance present in a given measures the intensity of transmitted light.
volume of solution, typically expressed in units such 17. Random fluctuations in the measurement signal
as molarity (M) caused by various factors such as electronic
13. A measure of how strongly a substance absorbs interference or stray light.
light at a particular wavelength, with units of
/mol-cm
15. The fraction of light that passes through a
sample
16. The point at which the graph of absorbance
versus concentration intersects the y-axis ( A-
axis)
18. A device that isolates light of a specific
wavelength from a broad spectrum.
19. A graph plotting absorbance or transmittance as
a function of wavelength
