SPERM STAIN READY TO USE
For "in vitro" diagnostic
Principle
3- Stain the smear by immersion in the working
The SPERM STAIN READY TO USE constitutes a system to Reagent Nr. 3 ; 5 times, 1 second each.
differentiate the morphological structures of the spermatozoa for a
perfect functional assessment. It can also be used for the Rinse the smears gently with deionised water and leave them
differential staining of blood cells in semen. The stains which make for air-drying.
up the SPERM STAIN READY TO USE combine polychromy and
the quality of classic cytology staining methods (May-Grünwald,
Giemsa, Wright) with a very quick execution (time of just 15
seconds). The technique is performed by immersion in the staining
solutions.
Product characteristics
The SPERM STAIN READY TO USE system is based on the
original Romanowsky staining method for differential staining of
several cellular structures.
Reagents
Kit 3 x 500 ml. (Ref. 98 30 95). Content:
1. SPERM STAIN READY TO USE NR. 1
1 x 500 Ml (Ref. 98 16 85)
Hexamethyl-p-roseniline methanolic solution. Handle with care.
2. SPERM STAIN READY TO USE NR. 2 In order to prevent deterioration of the smears by the
immersion oil coversliping is recommend with an appropriate
1 x 500 mL (Ref. 99 42 43) mounting media such as DPX, Eukitt ®. Use two drops and a
Xanthene buffered solution. standard glass coverslip ( 50 x 22 mm ).
3. SPERM STAIN READY TO USE NR. 3
Remarks
1 x 500 mL (Ref. 99 24 29)
Thiazine buffered solution Staining intensity can be modified by varying the number of
the immersions in solutions 2 and 3, depending on what colour
is preferred to be emphasized.
Working reagents Curettes with stain shall always be stored capped, specially 1
All the reagents are ready for use. in order to avoid undesirable evaporations that could promote
colour deviations from the usual stainings.
Storage and stability
Results
The components of the kit, stored at room temperature (15 - 25º C) Sperm head: Dark violet
will remain stable until the expiration date stated on the label. The Sperm acrosome: Pale violet, clearer then the head colour
working reagents are stable at room temperature (15 – 25 º C) for a
minimum of 3 months when reagents contamination or excessive Midpiece and tail: dark violet
evaporation are avoided. Background: Pale pink
Caution Red blood cells: Pale or deep pink
Reagent nº 1, as a methanolic solution is flammable and toxic by Neutrophils: Dark blue nucleus. Pink cytoplasm with red-violet
contact, inhalation and ingestion. Handle with care. Waste granulations
products must be handled attending your local regulations. Eosinophils: Blue nucleus. Blue cytoplasm with red or red-
orange granules
Basophils: Dark blue or purple nucleus. Purple, almost black,
Sample preparation granules
Sperm samples: prepare the smear with 15 µl of fresh sperm on a Lymphocytes: Violet nucleus. Sky blue cytoplasm
standard glass slide. Leave it for air drying at least 10 minutes. It is Monocytes: Very pale violet nucleus. Sky blue cytoplasm
recommended to make a thin and homogeneous spreading of the
sample for the best fixation of the dyes and avoiding blurring
hypercolorations. References
Gurr,E. (1965) "The rational use of dyes in Biology", p. 115.
Staining procedure Leonard Hill, London.
Gurr,E. (1971) "Synthetic dyes in Biology, Medicine and
1- Fix the smear by immersion in the Working Reagent Chemistry". Academic Press. London & New York
Nr. 1 ; 5 times, 1 second each. Let drain.
Maree, L.; du Plessis, S.S.; Menkvels, R. and van der Horst, G.
2- Stain the smear by immersion in the Working Human Reproduction, 25 (6), 1369 – 1382 (2010).
Reagent Nr. 2 ; 5 times, 1 second each.
Let drain again.
MICROPTIC S.L.
Av. Josep Tarradellas, 8, 1º 6ª
08029 - Barcelona – Spain www.micropticsl.com
Tel: +34 93 419 29 10
Manufactured by
QUIMICA CLINICA APLICADA S.A. for MICROPTIC S.L.
A 7 Km 1081 – Apdo. 20 - 43870 AMPOSTA / España Rev: 04/01/2018
