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VTM Note

Viral Transport Medium (VTM) is designed for the non-propagating transport of viruses from clinical specimens for viral particle measurement. It contains Hanks’ Balanced Salt Solution, fetal bovine serum, and antibiotics, and is intended for in vitro diagnostic use only. VTM has a shelf life of 12 months when refrigerated and requires proper specimen collection and handling procedures to ensure successful viral isolation.

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57 views2 pages

VTM Note

Viral Transport Medium (VTM) is designed for the non-propagating transport of viruses from clinical specimens for viral particle measurement. It contains Hanks’ Balanced Salt Solution, fetal bovine serum, and antibiotics, and is intended for in vitro diagnostic use only. VTM has a shelf life of 12 months when refrigerated and requires proper specimen collection and handling procedures to ensure successful viral isolation.

Uploaded by

kirankumarrebaka44
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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VTM -Viral Transport Medium

is a culture medium designed for the non-Propagating transport of viruses


from clinical patient specimens for the Measurement of viral particles using
molecular or antigen assays.

SUMMARY
Routine procedures in the diagnosis of infections caused by viruses Involves
the collection and refrigeration of biological specimens.

VTM (Viral Transport Medium) contains: FOR IN VITRO DIAGNOSTIC USE ONLY

1.Hanks’ Balanced Salt Solution


2.Fetal bovine serum
3.GentamicinAmphotericin B

# VTM is manufactured according to CDC- Canters for Disease Control and Prevention

PRECAUTIONS: -
VTM is a single-use device for trained and qualified professional use only.
VTM is not hazardous and does not require special methods of disposal and handling.
Take appropriate precautions for infected material if necessary.
For transport use only, not to be taken internally.

STABILITY AND STORAGE:-


VTM has a shelf life of 12 months from the date of manufacture when stored at the refrigerator
temperatures (2–8ºC). Alternatively, VTM can be stored at room temperature (15–28ºC) for up to 6 months.
Do not freeze or overheat.

1. -80° C: 24 hours
2. 2-8° C: 4 weeks
3. 25° C: 4 weeks
4. 37° C: 24 hours

QUALITY CONTROL:-
VTM is tested for pH and sterility. Sterility is assessed by applying the solution to the surface of sheep blood
agar plates and incubating for 48hours at 35 ± 2°C checking for growth daily
SPECIMEN COLLECTION AND PREPARATION:-
1. Specimens should be collected and handled using protective equipment against biological risk according
to published manuals and guidelines.
2. Proper collection of the specimen from the patient is crucial for the successful isolation and identification
of infectious viruses.
3. In order to maintain optimal microorganism viability, transport the specimens to the laboratory as soon
as possible.

PROCEDURE:-
Materials Provided: Each vial contains the volume of VTM as stated on the label.
Materials Not Provided: Collection devices or swabs, materials or devices for the identification of viruses.

SPECIMEN PROCESSING:-Due to the hazardous nature of patient specimens, all standard


laboratory safety procedures should be observed during all phases of collection.
1. After collecting the specimen, unscrew and remove the cap from the VTM vial, taking
care not to spill the medium.
2. Insert the swab into the vials until the swab breakpoint (if present) is level with the vial
opening.
3. Bend and break the swab at the breakpoint holding the vial away from your face. If the
swab does not have a breakpoint, cut the excess shaft and discard
4. Replace cap and tighten.
5. Process the specimen contained in the VTM within 48 hours of collection, following
local laboratory guidelines regarding transportation and storage temperatures.

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