CAMILA Time : 3 Hours Answer any eight of the following questions. Each quk 9 ew DE 1. 12. . What is transamination ? Give an example VI Semester B.Sc. Examination| US — 339 May 2017 (CBCS) (Fresh) (2016-17 and Onwards) CHEMISTRY Paper — VIII : Biochemistry Instructions : 1) The question paper has two P: 2) Write diagrams and equations| PART-A Mention the contributions of the following scienti Biochemistry : i) Emil Fischer li) Francis Crick. Write Haworth structure of glucose-6-phosphate. What are essential amino acids ? Give two examp| What is saponification value of an oil or fat ? What| What are nucleotides ? Give an example. Give an example of an amino acid derivative horm| role. Explain energy coupling reaction with an example. What is absolute specificity ? Give an example. Give the principle of thin layer chromatography. How are fatty acids activated ? Write the equation. Write the central dogma of molecular biology. Max. Marks : 70 irts. Answer both the Parts. wherever necessary. iestion carries two marks. (8x2=16) ts to the development of les. does it signify 7 ne. Mention its biological PTO. ALOOUE - Sn odUS - 339 Answer any nine of the followi 13. 14, 15. 16. 17, 18. 19, 20. a) Give the biochemical b) Write the principle an (PAGE). a) Write the partial struc does it differ from star 2 (ARNE PART -B 1g questions. Each question carries six marks. (9x6=54) nd elementary composition of living organisms. applications of polyacrylamide gel electrophoresis (343) lure of glycogen. Give its biological importance. How in? b) What are sugar phosphates ? Name the sugar phosphate present in RNA. (442) a) What are triglyceride: b) Write the structure of a) What are hormones 4 i) Glucagon il) Vasopressin ili) Testosterone. b) Mention the clinical si Density Lipoproteins a) How are proteins clas for each. b) Write the structure of a) Write a note on i) o-helix 7 Mention their biological importance. lecithin. (442) Give the biological functions of : nificance of Low Density Lipoproteins (LDL) and High (HDL). (442) ified based on biological function ? Give an example proline. (4+2) il) B-pleated structufe of proteins. b) What is protein dena} a) Describe Watson an{ uration ? How is it caused ? (442) Crick model of DNA. b) Write a note on chrotnatin. (442) a) Write a note on : |) Koshlanc’s induded fit theory of enzyme catalysis il) Effect of pH on enzyme catalysis. ») Write Michaelis - Menten equation and mention the terms involved. (4+2)‘SELL * US - 339 21. a) Explain substrate level and oxidative phospholylation. Give an example for 22. 23. 24, 25. substrate level phosphorylation. b) Write a note on active site of an enzyme. a) Give the schematic representation of electron sites of ATP synthesis. b) What is gluconeogenesis ? a) i) Write equation for the conversion of gly 1,3 - di phosphoglycerate. ii) Give the significance of urea cycle. b) How is pyruvate converted into ethanol in yea! a) Calculate the number of ATP molecules produ CoA in TCA cycle. b) Write equation for the conversion of fumarate a) Explain the role of the following in DNA replic i) DNA gyrase il) DNA helicase. ill) DNA ligases iv) DNA polymerase I. b) What is a codon ? Write the initiation codon. (442) ransport chain. Indicate the (442) raldehyde-3-phosphate to t? (442) ied by the oxidation of acetyl lo malate. (442) tion (442) LipRary‘8 SM - 355 VI Semester B.Sc. Examination, May/June 2018 (CBCS) (Fresh + Repeaters) (2016-17 and Onwards) Paper - Vill: CHEMISTRY Biochemistry Time : 3 Hours Max. Marks : 70 Instructions: 1) The question paper has two parts. Answer both the Parts. 2) Write equations and diagrams wherever necessary. PART-A : ‘Answer any eight of the following questions. Each question carries two marks. (8x2=16) 1, Mention the contributions of the following scientists to the development of biochemistry. i) Louis Pasteur ii) Hans Kreb. . Write the Haworth structure of N-acetyl B-D-glucosamine. . Write the structure of Lysine. . Define saponification number. Give its significance. . What are allostearic enzymes ? Give an example. . Give the structure of a nucleotide present only in RNA. . Give the principle of electrophoresis. . Explain energy coupling in biological systems with a suitable example. © MPnonp wn |. What is glycolysis 7 10. Explain group specificity with an example. LIBRARY Surana colleng 11. Write the structure of glycylalanine. No. 16. South End Bean, “a BANGALORE - 560 004 12. What are liposomes ? Give an example.SM-355 4 CL PART ~—B ‘Answer any nine of the following questions. Each question carries six marks. (9x6=54) 13. 20. at. a) Discuss the properties of water which makes it the solvent of life. ) Give the principle of thin layer chromatography. Mention two applications. (343) a) Name the storage polysaccharide present in animals and give its partial structure. How does it differ from amylopectin ? b) Write the structure of o-D-fructose 1, 6-diphosphate. (442) .. a) i) Write the structure of phosphatidyl choline. ii) Mention the biological functions of phospholipids. b) Give the biological role of HDL and LDL cholesterol. (442) a) Explain renaturation of protein in Aufinsen’s experiment with ribonuclease. b) Name the forces that stabilizes tertiary structure of a protein. (3+3) - a) How does the following factors affect rate of enzyme catalyzed reaction ? i) Effect of pH. ii) Effect of substrate concentration. b) Define active site of an enzyme. (442) }._a) How are proteins classified based on bidlogical functions 2 b) Give the reaction of an amino acid with alcohol. (442) . a) Write any one irreversible step of the glycolytic pathway. b) How is pyruvate activated prior to its entry into the TCA cycle ? ©) Calculate the total ATP produced by the complete oxidation of palmitic acid. (24242) @) Give the structural features of ATP, which makes it a high energy molecule. b) Explain Substrate level phosphorylation with a suitable example. (3+3) a) What are hormones ? How are they classified ? Give one example each. b) Expigin hormonal action mediated by cyclic AMP. (442)ccm ‘SM - 355 22. a) How are amino acids classified based on polarity of side chain ? b) Explain primary structure of a protein. (442) 23, a) Mention four salient features of Watson and Crick model of DNA. »b) Give the protein nucleic acid interaction in chromatin. (442) 24, a) Give the structure of two purine bases present in nucleic acids. b) Write the enzymatic reaction for the conversion of malate to oxaloacetate. (4#2) 25. a) Explain briefly the mechanism of DNA replication. b) Define translation. (442) LIBRARY. Surana College No. 16, South End Read. BAT GALORE - 580 09410066 Bn at Ped Doge : HAO GS-308 VI Semester B.Sc. Examination, May/June - 2019 CHEMISTRY Biochemistry-VIII (CBCS) (F+R) (2016-17 & Onwards) ‘Time : 3 Hours Max. Marks : 70 Instructions : (1) The question paper has two parts, Answer both the parts. (2) Write equations and diagrams wherever necessary. PART -A Answer any eight of the following questions. Bach question carries two marks. 8x2=16 1, Mention the contribution of the following scientists to the development of biochemistry (a) Han’s Krebs (b) Fredric Sanger 2. Write the Zwitter ionic structural form of glycine. 3. Define Michaelis - Menten constant. Mention’its significance. 4, Write Haworth structure of B-D-galactosamine. 5. What is energy Coupling ? Give an example. 6. Mention any two termination codons. 7. Define saponification number of lipids give its significance. 8. Write the reaction of urea cycle catalysed by arginase 9. Explain competitive inhibition, with an example. LIBRARY Surar 10, What are nucleotides ? Give an example. No. 16, South Ene Boog BAr GALORE - 560 004 11, Write the structure of alanyl serine. 12. Name the forces which stabilises tertiary structure of proteins, P.T.O.8-308 2 LOO 13. 14. 15. 16. 17. 18. 19. 20. PART - B Answer any nine of the following questions. Each question carries six marks, (a) Write the principle and applications of thin layer chromatography. ox6754 (b) Write any two biological role of water. (a) Write the partial structure of glycogen, give its biological importance. 4+2 How does it differ from starch ? (0) Give any 0wo biological role of Chitin. (a) What are fatty acids ? Give their classification with one example in 4+2 cach clas (b) Define liposomes. Give any two applications of it. {a} What are hormones ? Give the biological functions of. a2 (i) Glucagon (ii) Oxytocin (iii) Progesterone (b) Write a note on denaturation of proteins, (a) How are amino acid classi one example for cach class. (b) Write a note on Primary structure of protein, ‘d based on polarity of side chain ? Give 442 (a) (i) Name two aromatic amino acids, 24242 (ii) Name two hydroxy! group containing amino acids. (b}_ Define Isoelectric pH. Write the ionic forms of alanine at pH, ; (c} How do amino acids reacts with alcohol ? Write note on the following, +242 (a) Koshland’s induced fit theory of enzyme catalysis. (b), Effect of temperature on enzyme catalysed reaction, {c)’ Active site of an er (a) Calculate the number of ATP molecules produced by the oxidation of 442 acetyle CoA in ‘TCA eyele. (b) Write equation for the conversion of fumarate to malate.IO 7 os-208 21. 22. 23. 24. 25. fa) (b) {c) fa) (b) (a) fb) fa) (o) (a) () te) Describe the role of carnitine in the metabolism of fatty acid. 24242 How is pyruvate converted in to ethanol in yeast ? Write the reaction of glycolytic path way catalysed by Aldolase. Why is ATP the most efficient energy molecule ? Illustrate with 442 structure. What is substrate level phosphorylation ? Give an example. Explain semiconservative mechanism of DNA replication. 442 What is translation ? Mention four salient features of Watson and Crick model of DNA. 442 Describe Chargafi’s rule of base equivalence. Write a note on Chromatin. 24242 What is transamination ? Give an example. Write any two applications of DNA finger printing. LIBRARY -000- yo, Surgra College No. 16, South End At 84"'GALORE - 560 004SG - 272 VI Semester B.Sc. Examination, September/October 2021 (CBCS) (F+R) (2016-17 and Onwards) (CHEMISTRY (Paper - Vill) Bio-Chemistry Te 8 Hous Max. Mats: 70 Inaructions: 1) The question paper has two Pats. Answer bot the 2 ite grams and equations wherever necssay PART =A Aroneany eight othe foloing uso. Each questo cars wo mas: (x2=16) 1. Mention the major contributions of each of the following biochemists in the development of biochemistry i) Emi Fischer i) Lavosier 2. Wit the Haworth structure of somatose. ‘3, Why phospholipids are called amphipathic compounds ? siIBRany 4, Write the Zwitterionic form of alanine SaSce eae Read ‘The two strands of DNA are antiparallel. Explain Define Michaelis Menten constant. Give its significance. What is P/O ratio? How areffatty acids activated ? Write the equation. 6. 7 8. How is pyruvate converted to ethancl ? °. 10. Write the principe involved in electrophoresis 11. Give any two applications of DNA finger printing, 412. Name the hormones involved in blood sugar regulation. Pro.8G-272 2 mua PART-B [Answer any nine of the following questions. Each question carries six marks: (9x6=54) 18, a) Discuss the principle and applications of paper chromatography. 'b) Water's the medium of ite. Comment. (2) 14, a) What are the components of starch ? Indicate the structural diferences between them. ) Write the partial structure of chitin. (42) 15, a) What are lipids ? How are they classified ? Give example for each class. 'b) Write the structure of Cephatin (42) 16, a) Whats the biological role of epinephrine ? Where its produced ? 'b) What are liposomes ? Give its significance. (2) 17, ) How does amino acids react wth 1) HNO, wea $ Sey Give te castcaton of protsins based onthe structure wth examples (442) S78; a) Whats a peptide bond ? Why its planar in nature ? ) What's denaturation of proteins ? 42) 19. a) Witte the otferentypes of RNA and their biological roles. ») What's the diference batween nucleoside and nucleotide ? (42) 20, a) Explain diferent types of specticty exhibited by enzymes with example ») Explain Koshland induced ft theory of enzyme action. (42) 21, a) Describe the organisation of electron cartes inthe mitochondrial electron transport chain diagrammatically ) Whatis an apoonzyme and halo enzyme ? (42)8G -272 22. a) Give the equation forthe reduction of acetaldehyde by NADH. ») Give any two differences between oxidative phosphorylation and substrate phosphorylation, ©) Whats ransamination ? Give example, (24242) 23. a) Give the sequence of reactions by which an activated faty acid undergo Broxidation in mitochondria, ) How does pyruvate formed during glycolysis enterthe TCA cycle ? (442) 24, a) Write the reaction of TCA cycle catalysed by () citrate synthetase (I) Malate dehydrogenase. b) Write the reaction of urea cycle where urea is formed. (42) 25. a) Outline the semi conservative mode of replication of DNA, b) Whats transcription ? (42) LIBRARY Surana Colege No. 16, South End Road, GANGAL ORE - 560 004,