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Paper 102

This document outlines the examination details for the Bachelor of Science program in Microbiology, specifically for the Theory Paper II on Microscopy and Staining Techniques. It includes instructions for students on how to fill out their answer books, the structure of the exam with questions categorized by type and marks, and specific topics to be covered in the answers. The exam is scheduled for February-March 2024 and consists of various questions related to microscopy techniques.
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0% found this document useful (0 votes)
64 views2 pages

Paper 102

This document outlines the examination details for the Bachelor of Science program in Microbiology, specifically for the Theory Paper II on Microscopy and Staining Techniques. It includes instructions for students on how to fill out their answer books, the structure of the exam with questions categorized by type and marks, and specific topics to be covered in the answers. The exam is scheduled for February-March 2024 and consists of various questions related to microscopy techniques.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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2303000501026001

EXAMINATION FEBRUARY-MARCH 2024


BACHELOR OF SCIENCE (NCF-NEP)(FIRST SEMESTER)
MAJOR-2-MICROBIOLOGY PAPER - II THEORY
(MICROSCOPY AND STAINING TECHNIQUES) -
LEVEL 2(MB-MJ-102)

[Time: As per schedule ] [Max. Marks: 35]

Instructions: Seat No:


1. Fill up strictly the following details on your answer book
a. Name of the Examination : BACHELOR OF SCIENCE (NCF-
NEP)(FIRST SEMESTER)
b. Name of the Subject : MICROBIOLOGY PAPER - II THEORY
(MICROSCOPY AND STAINING TECHNIQUES) LEVEL 2
(MB-MJ-102)

c. Subject Code No : 2303000501026001


2. Sketch neat and labelled diagram wherever necessary. Student’s Signature
3. Figures to the right indicate full marks of the question.
4. All questions are compulsory.

Q.1 Answer in very short. (Any Five) 5

A. Give the source of light used in TEM.


B. State the magnification achieved by scanning tunnelling microscope.
C. Give one application of dark-field microscopy.
D. State any two points describing importance of fixation while using
bright-field microscope.
E. Give an example of acidic and basic dyes each.
F. Give two functions of ocular lens.

Q.2 Answer the following in brief. (Any Two) 10

A. What is numerical aperture? Discuss it in detail.


B. Explain working difference with reference to uncovered and covered
objects.
C. Discuss mordants and intensifiers as important components of staining.

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Q.3 Answer the following in brief. (Any Two) 10

A. Discuss principle, working and uses of phase contrast microscope.


B. Discuss DIC and CSLM microscopy techniques.
C. Discuss Gram staining technique.

Q.4 Answer the following in detail. (Any one) 10

A. Discuss detailed specimen preparation techniques of TEM.


B. Explain SEM and electron cryotomography microscopies in detail.

*****

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