2303000501026001
EXAMINATION FEBRUARY-MARCH 2024
BACHELOR OF SCIENCE (NCF-NEP)(FIRST SEMESTER)
MAJOR-2-MICROBIOLOGY PAPER - II THEORY
(MICROSCOPY AND STAINING TECHNIQUES) -
LEVEL 2(MB-MJ-102)
[Time: As per schedule ] [Max. Marks: 35]
Instructions: Seat No:
1. Fill up strictly the following details on your answer book
a. Name of the Examination : BACHELOR OF SCIENCE (NCF-
NEP)(FIRST SEMESTER)
b. Name of the Subject : MICROBIOLOGY PAPER - II THEORY
(MICROSCOPY AND STAINING TECHNIQUES) LEVEL 2
(MB-MJ-102)
c. Subject Code No : 2303000501026001
2. Sketch neat and labelled diagram wherever necessary. Student’s Signature
3. Figures to the right indicate full marks of the question.
4. All questions are compulsory.
Q.1 Answer in very short. (Any Five) 5
A. Give the source of light used in TEM.
B. State the magnification achieved by scanning tunnelling microscope.
C. Give one application of dark-field microscopy.
D. State any two points describing importance of fixation while using
bright-field microscope.
E. Give an example of acidic and basic dyes each.
F. Give two functions of ocular lens.
Q.2 Answer the following in brief. (Any Two) 10
A. What is numerical aperture? Discuss it in detail.
B. Explain working difference with reference to uncovered and covered
objects.
C. Discuss mordants and intensifiers as important components of staining.
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Q.3 Answer the following in brief. (Any Two) 10
A. Discuss principle, working and uses of phase contrast microscope.
B. Discuss DIC and CSLM microscopy techniques.
C. Discuss Gram staining technique.
Q.4 Answer the following in detail. (Any one) 10
A. Discuss detailed specimen preparation techniques of TEM.
B. Explain SEM and electron cryotomography microscopies in detail.
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