Introduction of Histopathology
SURESH KUMAR MAHASETH
LECTURER (MLT)
DSEU,DWARKA CAMPUS
suresh.k.mahaseth@dseu.ac.in
Introduction Of Histopathology
It is a branch of pathology which deals with the study of disease in a tissue section .
histopathology is defined as a three greek words: histos "tissue", pathos "suffering", and -logia "study .
The tissue undergoes a series of steps before it reaches the examiners desk to be
thoroughly examined microscopically to arrive at a particular diagnosis.
It is important that the tissue must be prepared in such a manner that it is sufficiently
thick or thin to be examined microscopically and all the structures in a tissue may be
Differentiated.
Histopathology laboratory is a unit performing clinical
activity associated with diagnosis during and post surgery.
The lab examines tissue materials, based on which an
emergency diagnosis is needed during surgery (i.e. frozen
section procedure), and then, based on the result, clinicians
determine the range of the surgery.
Most often this occurs when there is suspicion of a malignant
tumor. If the tumor is confirmed, a radical surgery is carried
out, avoiding making two interventions which would
significantly traumatize the patient.
Histopathology enables professionals to look for changes in cells that
explain the actual cause of the patient's illness. Pathologists are able
to reach a diagnosis by examining a small piece of tissue from various
organs. Histopathology is vital as it broadens and progresses treatments
options
Histopathology is the branch of pathology which concerns with the
demonstration of minute structural alterations in tissues as a result
of disease. Most of histopathological techniques simulating to those
of applied for study the normal histological structures.
Design of Histopathology Laboratory
It should be consist of three rooms, each one prepared
with proper ventilation, good light, proper
distribution of equipment and proper storage of
chemicals and reagents.
It should be prepared with suitable furniture.
Components of Histopathology Laboratory
I. Equipment and apparatus: include the following:
Microtome: Use to cut section.
Hot air oven: Use for impregnation of section
Tissue processing machine: Use for tissue processing.
Water bath: A laboratory water bath is used to heat sampl
es in the lab
Wax dispenser: Use for embedding the section
Cryostat: Use to cut the frozen section
Centrifuge: a machine with a rapidly rotating container that appl
ies centrifugal force to its contents, typically to separate fuids of
different densities.
Microscope: A microscope is an instrument used to see objects that
are toos mall to be seen by the naked eye
Safety cabinet: Is an enclosed, ventilated laboratory workspace for
safely working with materials contaminated with pathogens
requiring a defined biosafety level.
Electronic balance: is a balance use for weighting powders
Ware: general term use for glass and plastic materials which
includes tissue box, mold, bottles, specimen containers,
volumetric glass and cylinder.
Chemicals and reagents: It include xylene, alcohol, etc.IV. Dy
es: such as haematoxylin, eosin, alcian blue. etc
