QUESTION CUM ANSWER PAPER FOR FIRST TERMINAL EXAMINATION:
CENTRE NUMBER : IN462
  NAME : ___________________________           CLASS /DIV.: 9 A B C D E           ROLL NO.: ________
  SUBJECT : Biology                             PAPER: VI                         DATE :
  DURATION : 1 Hr                                                                 MAX.MARKS : 40
                                                                                   FOR EXAMINERS'S USE
                                                                                                    OUT
                                                                                   Q. NO. MARKS
  READ THE INSTRUCTIONS GIVEN BELOW :                                                               OF
  1. Write your name and roll number in the spaces provided at the top of this     1               24
     page.                                                                         2               16
  2. Answer all questions in the spaces provided on the Question Paper. Write
     in dark blue or black pen.                                                    3
  3. You should show all your working in the Answer Booklet. You may use a         4
     soft pencil for rough working.                                                5
  4. The Number of marks is given in the bracket '[ ]'
  5. This document consists of 10 pages. (9 printed + 1 blank )                    6
  6. Do not use staples, paper clips, highlighters, glue or correction fluid.      7
  7. At the end of the examination, fasten all your work securely together.        8
  8. You may use an electronic calculator.
                                                                                   9
                                                                                   10
                                                                                   11
                                                                                   12
                                                                                   13
                                                                                   14
                                                                                   15
                                                                                   16
                                                                                   17
                                                                                   18
                                                                                   19
                     NAME             SIGNATURE                 DATE
                                                                                   20
  INVIGILATOR :_____________           __________          _________               21
                                                                                   22
  EXAMINER :     _____________         __________           _________
                                                                                   23
  MODERATOR :_____________             __________          __________              TOTAL           40
                                                  BIO-6/I/09/
                         (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                  PAGE:1
        QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
1. Catalase is an enzyme found in many plants. It speeds up the break down of hydrogen peroxide
into water and oxygen, as shown in Fig. 1.1.
                                              Fig. 1.1
The oxygen produced during the reaction forms a foam on the surface of the liquid. The height of
the foam can be used as an indication of the activity of catalase.
(a) A student investigated the activity of catalase in three different types of plant tissue: seeds,
roots and fruits.
 The student used this method:
 Step 1 Put one seed into a test‑tube labelled S1.
 Step 2 Cut a small piece of root and place it into a test‑tube labelled R1.
 Step 3 Cut a small piece of fruit and place it into a test‑tube labelled F1.
 Step 4 Put 5cm3 of 6% hydrogen peroxide solution into each test‑tube.
 Leave for three minutes.
 Step 5 After three minutes, use a ruler to measure the height of the foam produced in each test‑tube.
 The student’s results from step 5 are shown in Fig. 1.2.
Step 6 Repeat step 1 to step 5 with fresh plant tissues and fresh hydrogen peroxide solution to get a
    second set of results.
                                                                                       PAGE TOTAL
                                            BIO-6/I/09/
                         (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                 PAGE:2
        QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
Fig. 1.3 shows the second set of test-tubes from step 6 after three minutes.
                                        Fig. 1.3
(i) Prepare a table to record the two sets of results and the average height of the foam
  for each tissue. Measure the heights of the foams in the three test‑tubes in Fig. 1.3. Calculate
  the average height of the foam for each tissue. Include in your table:
• the student’s results from Fig. 1.2
• the heights of the foams you have measured from Fig. 1.3
• the average heights you have calculated.
                                                                                                ___/[5]
                                                                                    PAGE TOTAL
                                             BIO-6/I/09/
                        (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                              PAGE:3
            QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
(ii) State a conclusion for these results.
      ...........................................................................................................................................
      ...........................................................................................................................................
      .....................................................................................................................................    ___/[1]
(iii) Identify one potential source of error in this investigation.
      ...........................................................................................................................................
      ...........................................................................................................................................
      .....................................................................................................................................    ___/[1]
(iv) The student collected two sets of results for each plant tissue. Explain why it would have been
      better to have collected three sets of results.
      ...........................................................................................................................................
      ...........................................................................................................................................
      .....................................................................................................................................    ___/[1]
(v) State two variables that should have been kept constant during this investigation.
1 ........................................................................................................................................
      ...........................................................................................................................................
2 ........................................................................................................................................
      ........................................................................................................................................... ___/[2]
(vi) State the variable that was measured (the dependent variable) in this investigation.
      .....................................................................................................................................         ___/[1]
(vii) Identify one safety risk when carrying out this investigation and describe how the risk could be
      reduced.
     risk .....................................................................................................................................
     method of reducing risk .....................................................................................................
      ........................................................................................................................................... ___/[2]
                                                                                                                                  PAGE TOTAL
                                                                 BIO-6/I/09/
                                    (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                                                                    PAGE:4
         QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
(b) A student wanted to calculate the rate of gas production but could not do this using the height of
    the foam.
    Describe how the student could change the method to collect the data they needed and state how
    they would calculate the rate of gas production.
    change to method .....................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    how the rate would be calculated .............................................................................................
    ...................................................................................................................................................
    ...............................................................................................................................................___/ [2]
(c) (i) The student added iodine solution to samples of the three types of plant tissue. The student
    observed the colour of the iodine solution on the plant tissue after a few minutes.
Their observations are shown in the table.
Complete the table by writing a conclusion for each observation.
                                                                                                                                         ___/[2]
(ii) State the name of the reagent that would be used to test a seed for protein.
    ..................................................................................................................................... ___/[1]
                                                                                                                                PAGE TOTAL
                                                               BIO-6/I/09/
                                  (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                                                               PAGE:5
         QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
(d) A student stated that: ‘Different types of plant tissue will lose different amounts of water by
    osmosis when immersed in a salt solution.’ Plan an investigation to find out if the student was
    correct.
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ...................................................................................................................................................
    ............................................................................................................................................. ___/[6]
                                                                                                                                     ____/[Total: 24]
                                                                                                                                PAGE TOTAL
                                                               BIO-6/I/09/
                                  (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                                                               PAGE:6
            QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
2 Cabbage leaves contain vitamin C. Boiling the cabbage in water reduces the vitamin C content of
the leaves. Some students investigated the effect of boiling time on the concentration of vitamin C
remaining in the cabbage leaves.
(a) The students prepared the cabbage samples using this method:
 Step 1 Heat a large volume of water until it begins to boil.
 Step 2 Measure the mass of chopped cabbage leaves.
 Step 3 Place the chopped cabbage leaves into the boiling water.
 Step 4 Continue to boil the water.
 Step 5 Remove a sample of cabbage leaves from the water at each time interval.
State two pieces of apparatus, other than safety equipment, that would be required to carry
out the method described in steps 1 and 2.
1 ................................................................................................................................................
2 ................................................................................................................................................___/[2]
(b) The vitamin C concentration of each cabbage leaf sample was then determined.
 The results of the investigation are shown in Table 2.1.
                                                                                                                                  PAGE TOTAL
                                                                 BIO-6/I/09/
                                    (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                                                                 PAGE:7
         QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
                                                            Table 2.1
(i) Plot a line graph on the grid of the data in Table 2.1.
                                                                                                     ___/[4]
 (ii) Use your graph to estimate the concentration of vitamin C in a sample of cabbage leaves
that has been boiled for 20 minutes.
 Show on your graph how you obtained your estimate.                                              ___/[2]
                                                                                           PAGE TOTAL
  .......................................... mg per 100g
                                                           BIO-6/I/09/
                               (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                      PAGE:8
            QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
(iii) Table 2.1 shows that at the start of the investigation 100g of unboiled cabbage leaves
contained 34mg of vitamin C.
 Using the information in Table 2.1, calculate the percentage decrease in the vitamin C
content of 100g of cabbage leaves after boiling for 25 minutes.
 Give your answer to two significant figures.
 Space for working.
                                                                ............................................................ %             ___/[3]
(c) The students wanted to know if the vitamin C had moved from the cabbage leaves into the
water that the leaves were boiled in.
(i) State how the students could test the water for the presence of vitamin C.
 Include the result of a positive test.
 ...................................................................................................................................................
 ...................................................................................................................................................
 ...................................................................................................................................................
 ............................................................................................................................................. ___/[2]
(ii) Describe how the students could test cabbage leaves for the presence of reducing sugars.
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
............................................................................................................................................. ___/[3]
                                                                                                                                 ____/[Total: 16]
                                                                                                                                  PAGE TOTAL
                                                                  BIO-6/I/09/
                                     (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                                                                                  PAGE:9
       QUESTION CUM ANSWER PAPER FOR:FIRST TERMINAL EXAMINATION:
                                     BLANK PAGE
                                                                             PAGE TOTAL
                                   BIO-6/I/09/
                 (For Private Circulation Only. This Material is not Copyright Free)
F/M&M/EP/4.2
                                                                                       PAGE:10