Centrifuge 5430 R

Service manual - English

5428 920.017
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the
copyright owner.
Trademarks

Eppendorf®, the eppendorf logo and CombiSlide® are registered trademarks of Eppendorf AG.

Centriplus® is a registered trademark of Millipore Corporation, Billerica, USA.

Falcon® and Microtainer® are registered trademarks of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or ® in this manual.

5428 920.017-06/022013
 Centrifuge 5430 R — Service manual - English

Table of contents
Table of contents
Service
Centrifuge
on
see
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Tab.
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—p. manual5430 -REnglish

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Hardware and software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Front panel with display and PCB controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.2 PCB converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.3 PCB imbalance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.4 Drive motor and speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.5 Software update via the USB interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2 Safety-related routines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.1 Speed measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.2 Automatic rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.3 Overspeed protection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.4 Standstill detection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.5 Lid latch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.6 Imbalance detection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.2.7 Program monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.2.8 Power failures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3 Machine marking and serial number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.2 Liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.3 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.4 Safety instructions located on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
 Centrifuge 5430 R — Service manual - English

Table of contents

5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.1 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2 Error messages from service programs/other error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.3 Troubleshooting Error 30: Lid cannot be opened or closed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.4 Customer service functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.4.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.4.2 Activation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.4.3 40: Temperature and cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.4 41: PCB converter - cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.5 42: PCB converter - drive. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.4.6 43: Rotor detection and lid latch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.4.7 44: Imbalance detection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.4.8 45: Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.4.9 46: General device information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.5 Service functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.5.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.5.2 Activation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.5.3 Service function 1: Display test and key test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
5.5.4 Service function 2: Test the lid function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.5.5 Service function 3: Test rotor detection and rotor impulses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.5.6 Service function 4: Determine acceleration times and deceleration times. . . . . . . . . . . . . . . . . . . . 40
5.5.7 Service function 5: Initialization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.5.8 Service function 6: Test the overspeed cut-off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.5.9 Service function 7: Endurance test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.5.10 Service function 8: Error messages and data from the last 4 runs . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.5.11 Service function 9: Operating hours and number of load changes/cycles . . . . . . . . . . . . . . . . . . . . 42
5.5.12 Service function 10: Check USB interface using CefuComTest . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.5.13 Service function 11: Test individual units (fan/compressor) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.5.14 Service function 12: Run with adjustable constant compressor frequency . . . . . . . . . . . . . . . . . . . 44
5.5.15 Service function 13: Check imbalance detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.5.16 Service function 14: Machine test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.6 Rotor table and imbalance cut-off weights for Centrifuge 5430/5430R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.7 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
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6 Disassembly/assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.1 Tools and auxiliary equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.2 Upper lid part and monitoring glass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
6.2.1 Remove the upper lid part . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
6.2.2 Remove the monitoring glass. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
6.3 Lid axle and lid panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
6.3.1 Remove the lid axle and the lid panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
6.4 Lid latch nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
6.5 Magnet for lid position (actuator) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
6.5.1 Remove the magnet for lid position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
6.6 Gas spring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
6.6.1 Remove the gas spring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
6.6.2 Install the gas spring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
6.7 Front panel with PCB controller 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
6.7.1 Remove the front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
6.8 Console and emergency release guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.8.1 Remove the console . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.8.2 Remove the emergency release guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.9 PCB converter 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.10 Mains power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.10.1 Remove the mains power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.11 Brake resistor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.11.1 Remove the brake resistor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.11.2 Assembly of the brake resistor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.12 PCB imbalance/motor rotational speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.12.1 Remove the PCB imbalance/motor rotational speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.13 Fans for PCB converter 5430 R and motor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.13.1 Remove the fans for the PCB converter 5430 R and the motor. . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.14 Lid latch unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.15 Motor cap with insulating parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.15.1 Remove the motor cap with insulating parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.15.2 Install the motor cap with insulating parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.16 Temperature sensor of bottom of chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.16.1 Remove the temperature sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.16.2 Install the temperature sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.17 Centrifuge motor and motor suspension. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.17.1 Loosen the cable connections on the PCB Converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.17.2 Remove the centrifuge motor and motor suspension. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.17.3 Install the centrifuge motor and motor suspension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.18 Rear panel with USB interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.18.1 Remove the rear panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.19 Housing part . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.19.1 Remove the housing part. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.20 Chamber sealing ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.20.1 Remove the chamber sealing ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.20.2 Install the chamber sealing ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.21 Condenser fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.21.1 Remove the condenser fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.22 Upper part of housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.22.1 Dismantling the upper part of housing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.23 Temperature sensor on the condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.23.1 Replace the temperature sensor on the condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.24 Opening the centrifuge lid if the emergency release is defective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
 Centrifuge 5430 R — Service manual - English

Table of contents

7 Alignment/adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.1 Adjust the lid latch switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.1.1 Lid latch functionality . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.1.2 Requirements for setting switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
7.1.3 Setting the microswitch for the lid position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
7.1.4 Adjusting the right and left limit switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

8 Diagrams. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
8.1 Electronics layout plan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
8.2 PCB converter 5430R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

9 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
9.1 Cleaning and disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
9.1.1 Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
9.1.2 Cleaning agents and auxiliary equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
9.1.3 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
9.1.4 Cleaning and disinfecting the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
9.2 Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
9.3 Shipping of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
9.4 Centrifuge maintenance overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
9.5 Maintenance services for centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
9.6 Extra maintenance services for centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
9.7 Device tests for centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.7.1 Speed check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.7.2 Temperature check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.7.3 Timer check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
9.7.4 Electrical safety test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

10 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.3 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.4 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
11.1 Images of spare parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
11.2 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
11.2.1 Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
11.2.2 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
11.2.3 Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
11.2.4 Drive. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
11.2.5 Temperature sensors / fans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
11.2.6 Lid latch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
11.2.7 Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
11.2.8 Refrigeration system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
11.2.9 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
11.3 Rotors, rotor lids and gaskets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
11.3.1 Rotors with QuickLock rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
11.3.2 Rotors with rotor lid thread . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
11.3.3 Rotors with rotor lid for attaching . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
11.4 Suggested spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
11.4.1 Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
11.4.2 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
11.4.3 Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
11.4.4 Drive. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
11.4.5 Temperature sensors / fans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
11.4.6 Lid latch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
11.4.7 Mains supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

12 Technical information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

 Centrifuge 5430 R — Service manual - English

1 User instructions
1 User instructions
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

1.1 Using this manual

 Ensure that you have a copy of the latest versions of the service manual and operating instructions. Check this using
the version numbers at www.eppendorf-support.com and www.eppendorf.com.
 Read the service manual before starting work on the device.
 Read the chapters "Installation" and "Operation" of the operating manual.
 Observe the safety instructions in the operating manual.

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Biohazard Explosion

Electric shock Crushing

Hazard point Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning
 You are requested to perform an action.

1.
Perform these actions in the sequence described.
2.
• List.
References useful information.

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1 User instructions

1.4 Abbreviations used

MTP
Microplate
PCR
Polymerase chain reaction
PTFE
Polytetrafluorethylene
RZB/rcf

Relative centrifugal force – g-force in m/s2

rpm
Revolutions per minute – in rpm
UV
Ultraviolet radiation

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2 Product description
2 Product description
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

2.1 Hardware and software

The centrifuge electronics are made up of the following assemblies:

• Front panel with display and PCB controller
• PCB converter
• PCB imbalance
The cold appliance plug and the overcurrent protection switch are located on the right-hand side of the device.

2.1.1 Front panel with display and PCB controller

This assembly has the following components/functions:

PCB controller
• Input and output of speed and time
• Control of all run parameters
• Interface to the converter
• Loudspeaker control
• Display software (V_D) is installed.

The front panel is available in two designs:

• Rotary knobs – speed and time are input using two mechanical encoders. All other parameters are entered using arrow
keys.
• Keypad - all parameters are entered using arrow keys.

The front panels of Centrifuge 5430 R have been changed from serial number 10,000 upwards.
Hint! The new front panels can be installed in centrifuges with a serial number >10,000.
The new front panels are only approved for use with display software (V_D) version 3.0 or higher.
Old front panels are only compatible with display software version 1.0 to 1.2.
When the front panel is mounted, the "CefuProgrammer" can be used to determine which
software version is installed in the front panel. Both of the current display software versions up to
1.2 and from 3.0 onwards are available on our website at www.eppendorf-support.com under
"Software Downloads“.

Input elements:
• start/stop key
• open key
• Lid release key
• Switch rcf/rpm key
• menu enter key
• Arrow keys
• fast temp key
• Short key
• Five prog keys
Display
The parameters and symbols are displayed in an illuminated LC display.

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2 Product description

2.1.2 PCB converter

The PCB converter has the following parts:

1. Centrifuge control
• Hardware control unit on the PCB converter for the centrifuge run, with the software (V_C)
• Communication with PCB controller.
• Signal processing of the speed, rotor temperature, condenser temperature and imbalance sensors.
• Control of lid latch motors including detection of the lid switch. The centrifuge control controls the 12 V M2 and M3 gear
motors using the driver circuit. These motors move the spindles on the left and right lid latch units. The microswitch
signals for the S2 lid latch, S7 reed switch and S3 and S6 limit switches are evaluated. Using the signals, the PCB
converter detects whether the centrifuge is locked.

2. Drive control
• Interface for centrifuge control via optical coupler.
• Switching power supply unit for 12 V/5 V for controlling the PCB controller and the lid latch motors. Switching power
supply unit for 15 V for controlling the converter.
• Radio interference filter.
• Voltage doubler for 120 V.
• PFC circuit for 100 and 230 V.
• Braking chopper with external brake resistor.
• Module with control for drive motor.
• Hardware safety circuit for lid switch and motor temperature safety switch outside of processor control.
• Control unit on the PCB converter for centrifuge motor with the software (FUV).
The 230 V version centrifuge features an active power factor correction switch (PFC) for observance of the harmonic
standard EN 61 000 -3-2. It consists of IC N25 (UCC 3818D), MOSFET V63 and PFC choke L10/L32/L33, and ensures
that the mains current is nearly sinusoidal.
The 100 V version centrifuge also has a PFC switch. The harmonic content is also reduced here so that the mains current
is almost sinusoidal, providing the 100 V version with sufficient power for the drive.
The intermediate circuit voltage and the intermediate circuit current are monitored.
The current run parameters are stored on the PCB converter. The parameters remain available after the centrifuge has
been switched off.

2.1.3 PCB imbalance

• The PCB imbalance is located on the base of the drive motor.
• The imbalance detection is carried out using an acceleration sensor on the PCB imbalance.
• The motor rotational speed is measured with a Hall sensor on the PCB imbalance.

2.1.4 Drive motor and speed

The drive motor is a three-phase asynchronous motor ZCI 7.
• The acceleration and braking of the drive motor is controlled by a ramp.
• A Hall sensor measures the speed of the motor. The sensor is located on the PCB imbalance below the motor.
• The speed signal (one pulse per revolution) is used for speed control and display purposes.
• A digital Hall sensor measures the rotor speed and detects the rotor type. The sensor is located above the upper
A-end shield of the motor in the mounted motor cap.

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2.1.5 Software update via the USB interface

Mainboard software (V_C):
After the centrifuge has been switched on, the version of the centrifuge control software (V_C) is briefly shown in the
display. The centrifuge control software is located on the PCB converter; it consists of the V_C version software for the
control unit. This software is available as "mainboard software".
Centrifuge 5430 R has been modified from serial number 13,000 upwards. The new spare parts may only be used with
mainboard software from version 2.1 upwards.
The two current versions of the mainboard software are available on the website www.eppendorf-support.com.

Display software (V_D):

The "display software" (V_D) is installed on the PCB controller of the 5430R display/front panel.
2.1.5.1 Testing the USB interface

Prerequisites
• USB-A to USB-B connection cable, order no. 5428 850.280
• PC/laptop
1. Download the current version of the CefuComTest and the description of the test from the website
www.eppendorf-support.com.
2. Install and execute the CefuComTest program according to the description.
2.1.5.2 Software update
Perform the software update after you have replaced the PCB converter or the front panel with the PCB controller.

Prerequisites
• USB-A to USB-B connection cable, order no. 5428 850.280
• PC/laptop
1. Using customer service function 46, check which versions of the mainboard software (V_C) and display software
(V_D) are installed on the centrifuge.
2. Check to see if new versions of the software are available on the website www.eppendorf-support.com.
3. Download the current version of the CefuProgrammer and the description of the program from the website
www.eppendorf-support.com.
4. Download the current version of the mainboard software (V_C) and display software (V_D) from the
websitewww.eppendorf-support.com.
5. Install and execute the CefuComProgrammer program according to the description.

Customer programs will be stored on the PCB controller. Programmed user programs are
Hint! retained after the display software (V_D) has been updated.
If you have replaced the front panel with PCB controller, enter the programmed user programs
again.

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2.2 Safety-related routines

2.2.1 Speed measurement

The centrifuge has two rotational speed measuring systems (rotor and motor) that work independently of each other. They
are evaluated independently of one another, in interrupt-driven mode.
If both speeds fail during a run, error 3/2 is triggered. The drive is switched off and the lid can only be opened after 14 min.
Error 3 cannot be deleted by switching the device off and back on.

2.2.2 Automatic rotor detection

For each rotor, the maximum speed, cut-off speed (maximum speed +300 rpm) and maximum radius are saved.
Each rotor is identified by a start magnet and a code magnet with a different length (flexible magnet strips). The rotor with
the highest maximum speed has the longest coding magnet. If part of the coding magnet is demagnetized, the rotor will
only be able to achieve a lower maximum speed.
Rotor detection is carried out after each start-up to a speed of 700 rpm. Two successive measurements must produce a
valid measured value. Only then will the target speed be transmitted to the frequency converter. Simultaneously, a check
will be conducted to determine whether the target speed is less than the maximum speed of the detected rotor. If the target
speed is larger than the maximum speed of the rotor used, the run will be canceled with Error 1 and the target speed will
be reduced.

2.2.3 Overspeed protection

After the rotor detection, the maximum speed of the rotor will be transmitted to the frequency converter. A maximum
rotating field speed will be transmitted to limit the rotating field. Maximum rotating field speed = target speed + motor slip.
During the run, the rotor speed will be continuously measured and compared to the cut-off speed. The cut-off at overspeed
can be tested using a service function.

2.2.4 Standstill detection

The software divides a run into 3 phases:

• Phase 1: acceleration to target speed
• Phase 2: constant run
• Phase 3: braking until rest
The rotor speed and motor speed are continuously measured. If a standstill occurs in phase 1 or 2, Error 3 is triggered.
The current speeds, previous measured values, and the rotating field speed of the frequency converter are evaluated to
detect a safe standstill at the end of the deceleration time. The lid automatically opens after the standstill detection.

2.2.5 Lid latch

2 screws are used to keep the centrifuge closed. The depth of engagement is monitored by one limit switch at the left and
one at the right. The microswitch for the lid position and the reed switch provide a notification on whether the lid is open or
closed. The lid switches are polled in all operating states. If a switch opens during a run, the run is immediately canceled
and Error 30 is displayed.

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2.2.6 Imbalance detection

The acceleration sensor on the lower end shield of the motor measures the rotor imbalance. Speed-dependent cut-off
values (increment: 500 rpm) are defined for each rotor. If the cut-off value is exceeded, the run is canceled and Error 15
displayed.
The function of the sensor is tested using the sensor idle value at standstill and a minimum signal value during a run.

2.2.7 Program monitoring

After each switch-on the check sum of the software is checked. The watchdog function will be activated after the program
start. The watchdog function triggers a reset after undefined errors.

2.2.8 Power failures

The supply voltage to the processor is monitored by means of a reset circuit (TLE 7705).
If the voltage falls below 4.7 V, a reset is triggered. All connected components are switched to a safe state.
If voltage is applied, an acceleration program checks all components and determines their status. For example, the
rotating rotor will be braked until it comes to a standstill and, after that, the lid will be released and automatically opened. If
Error 3 was detected (saved on EEPROM), the lid remains closed for the saved remaining time.

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2.3 Machine marking and serial number

Abb. 2-1: Eppendorf AG machine marking using Centrifuge 5418 as an example

12 1

11 2

10 3

9 4

8 5

7 6

Fig. 2-1: Eppendorf AG machine marking using Centrifuge 5418 as an example

1 Manufacturer's address 2 Consecutive number within the 12-digit serial number

3 Maximum speed 4 Maximum kinetic energy

5 Maximum density of liquid 6 Quality seal

7 CE mark 8 Power consumption

9 Permitted frequency 10 Permitted voltage

11 Product number 12 Manufacturer

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3 Safety
3 Safety
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

3.1 User profile

The specialist entrusted with maintenance, repair or testing must meet the following prerequisites:
• Successful participation in service trainings with certification by Eppendorf AG for the product that is to be maintained,
repaired or tested.
• Qualification as a specialist with knowledge of the applicable local and international standards.
• Qualification to evaluate the work assigned to him.
• Recognition of potential hazards and how to prevent or correct them.

3.2 Liability
The Eppendorf service partner entrusted with maintenance, servicing or testing is liable for ensuring that all work is carried
out in a professional manner.
 The constructional features of the device must not be modified by servicing, maintenance or testing. The device must
always be as safe as in its original state.
 Only accessories and original spare parts recommended by Eppendorf as well as measuring and test equipment
recommended by Eppendorf may be used for any maintenance, servicing or testing work.

3.3 Safety notes

Electric shock.
 Switch off the device.
DANGER!  Unplug the device.
 Then begin repairing, servicing or cleaning the device.

Risk of explosion.
 Do not operate the device in areas where work is completed with explosive substances.
DANGER!  Do not use this device to process any explosive or highly reactive substances.
 Do not use this device for processing any substances which could generate an explosive
atmosphere.

Infection by contaminated material.

There may be contaminated material on the device and accessories.

WARNING!  Work must only be completed on decontaminated devices.

 Find out more about contamination risks before beginning work.
 Check the device decontamination certificate.
 Wear personal protective equipment (protective gloves, protective goggles).

Risk from incorrect supply voltage

 Only connect the device to power supplies which correspond with the electrical requirements
on the name plate.
WARNING!
 Only use sockets with a protective earth (PE) conductor and suitable power cable.

Crushing of the fingers with the centrifuge lid.

 When opening or closing the device lid, do not reach between the lid and device or into the
latching mechanism of the lid.
WARNING!
 Always open the centrifuge lid completely to prevent it from falling.

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3 Safety

Injury from sharp-edged components.

Sharp-edged components are exposed when the device is open.

CAUTION!  Wear protective gloves.

 Work with extreme care.

Risk of injury from chemically or mechanically damaged accessories.

Even minor scratches and cracks can lead to serious internal material amage.

WARNING!  Protect all accessory parts from mechanical damage.

 Inspect the accessories for damage before each use. Replace any damaged accessories.
 Do not use rotors, rotor lids or buckets with signs of corrosion or mechanical damage (e.g.,
deformations).
 Do not use any accessories which are past their use-by date.
 When inserting the buckets and rotors, ensure that they do not become scratched.

Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
CAUTION! accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.
 Only use accessories and original spare parts recommended by Eppendorf.

If an error occurs, the objects in the immediate proximity of the device will be damaged.
 In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30 cm
around the device during operation.
NOTICE!
 Please remove all materials and objects from this area.

Damage to electronic components due to condensation.

Condensate can form in the device after it has been moved from a cool environment to a warmer
environment.
NOTICE!
 After installing the device, wait at least for 3 h. Only then connect the device to the mains.

Damage to electrical components due to electrostatic discharge.

Handling electrical components creates electrical fields. The electrical components are destroyed
when these fields are discharged. Minimize the build-up of electrostatic fields.
NOTICE!
 Store and transport sensitive components and assemblies in antistatic or conductive packing.
 Wear grounding strips, antistatic clothing and antistatic safety boots.
 Use dissipative surfaces.
 Grip the component on the edges.
 Do not touch any protruding connections or conductors.
 Prevent components from becoming electrostatically charged on plastics.

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3 Safety

Device damage due to unprofessional repairing of the refrigerating system.

 The refrigerating system must be repaired by a refrigeration specialist.
NOTICE!  Repairs, e.g. spare parts replacement, are carried out on one's own responsibility.
 Observe national and local safety regulations.

Damage to the compressor and refrigerating system due to tilting the device.
The compressor and refrigerating system can become damaged if the device is switched on
when it is tilted or if the device is switched on right after it was tilted.
NOTICE!
 Leave the device in the normal position for at least 4 hours before switching it on.
 Never place the device upside down.

Damage as a result of incorrect packing.

Eppendorf AG is not liable for damage caused by improper packing.
NOTICE!  The device may only be stored and transported in its original packaging.

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3 Safety

3.4 Safety instructions located on the device

Display Meaning Location

Follow the instructions in the operating manual. 5430 R: Right side of the device

CAUTION Top of device, below the

centrifuge lid
Always tighten the rotor using the supplied rotor key.

CAUTION Top of device, below the

centrifuge lid
Close all tubes and use a rotor lid.

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4 Operation
4 Operation
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

4.1 Overview of operating controls

The centrifuge is available in two versions: keypad or rotary knobs. This manual generally describes how to operate the
keypad version. However, it also applies to the rotary knob version.
Abb. 4-1: Control panel and display of (keypad version).

Fig. 4-1: Control panel and display of (keypad version).

1 Select program 2 Short Spin centrifugation

Press briefly: load the stored centrifuging parameters.
Press and hold (> 2 sec): save the current centrifuging
parameters .

3 Release lid 4 Start and stop centrifugation

5 Activate/deactivate standby mode 6 Display

Key lights green: centrifuge is ready for operation.
Key lights red: standby mode active .

7 Set speed of centrifugation 8 Adjust the temperature (only 5430 R)

Dependent on device version, designed as key or rotary
knob.

9 Adjust the centrifuging duration 10 Start the temperature control cycle Fast Temp
(only 5430 R)
Dependent on device version, designed as key or rotary
knob.

11 Switch the displayed centrifuging speed (rpm/rcf) 12 Call and select the menu parameters

13 Navigating the menu

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4 Operation

Abb. 4-2: Display of the

Fig. 4-2: Display of the

1 Program number (if enabled) 2 Status of the function At set rpm

: Start of operation when reaching 95% of the preset

g-force (rcf) or speed.

: Start of run time immediately.

3 Status of the key lock 4 Status of the loudspeaker

: Centrifuging parameters cannot be modified : Switched on.

unintentionally.
: Switched off.
: No key lock.

5 Status of the centrifuge 6 Temperature control cycle programming (only 5430 R)

: Centrifuge lid unlocked. : Fast Temp pro is enabled, i.e., start time and
temperature of the temperature control run have been
: Centrifuge lid locked.
programmed .
(flashing): Centrifuging in progress.

7 Time 8 Soft ramp

: Rotor accelerates and brakes slowly.

No symbol: Rotor accelerates and brakes rapidly.

9 Standard display 10 Extended display (if enabled)

11 g-force/speed 12 Temperature (only 5430 R)

13 Centrifuging duration

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5 Troubleshooting
5 Troubleshooting
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

5.1 Error messages

If an error occurs, Eppendorf recommends that you install the current versions of the mainboard software and the display
software.
The numbers in the "Cause" column correspond to the possible error causes displayed by the centrifuge, (see 45: Error
messages on p. 35).

Symptom/message Cause Remedy

Error 1 • Rotational speed measuring system not  Check the rotational speed measuring
OK. system at the rotor.
Reaction: centrifuge does not start or  Check the motor for free movement.
brakes. Exchange the motor.
– 1: No rotor detected after  Test the centrifuge with a different rotor.
12 measurements. Check the magnets for rotor detection
– 2: Start magnet not detected or incorrect (customer service function 43: RR =
rotational direction of motor. rotor relation and RN = rotor number).
– 3: No rotor detected at n > 3000 rpm.  Check the rotor detection and rotor
impulses (service function 3).
– 4: Incorrect rotor detection or rotor not
recognized by outdated mainboard  Check/replace the motor cap with
software version integrated rotor sensor (customer
– 5. Breakdown of rotor rotational speed. service function 42: nR = rotor speed).
– 6: Breakdown of motor rotational speed.  Check/replace the PCB speed/
imbalance (customer service function
42: nM = motor rotational speed).
 Update the mainboard software.
 Replace the PCB converter.
Error 2 • Imbalance detection defective.  Switch off the centrifuge. Switch the
Reaction: centrifuge does not start or centrifuge back on after > 20 s.
brakes.  Check the sensor idle value. (Customer
– The imbalance idle value is outside of service function 44: US = sensor idle
the limits. value (see 44: Imbalance detection on
p. 35).
– The measured value is generally
incorrect.  Check/exchange the PCB imbalance/
motor rotational speed.
– 1: Sensor idle value < 1.5 V or > 3.5 V,
sensor defective.
– 2: Measured value does not change
during run.

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5 Troubleshooting

Symptom/message Cause Remedy

Error 3 • No rotor impulses during run or  Insert and tighten the rotor.
acceleration.
 Wait for the displayed time.
• There is no rotor.
 Test the rotor detection and rotor
• The rotational speed measuring system is impulses (service function 3).
defective.
 Check/replace the motor cap with
• The drive is defective. integrated rotor sensor (customer
– 1: No impulses after start. Reaction: service function 42: nR = rotor speed).
centrifuge brakes for 10 s.
 Check/replace the PCB speed/
– 2: Impulse failure during run. Reaction: imbalance (customer service function
centrifuge brakes; lid remains locked for 42: nM = motor rotational speed).
max. 8 min.
 Check the motor for free movement.
The error remains active even after a Exchange the motor.
mains/power failure. Error 3 will be
deleted after the idle period has  Replace the PCB converter, (see PCB
elapsed; lid will be released. converter 5430 R on p. 54).

Error 5 • No lid feedback during run.  Wait for the rotor to stop.
• 2: Reed switch open. Reaction: free  Check the lid latch (customer service
deceleration of centrifuge. function 43).
• 3: Limit switch open. Reaction: centrifuge  Check the lid function, adjust/replace the
brakes, drive switches off. switch if necessary (service function 2).
 Check/replace the reed switch.
 Replace the latch motor or lid latch unit.
 Replace the PCB converter.
Error 6 • Fault in the PCB converter, thermal  Switch off the centrifuge. Switch the
overload. centrifuge back on after > 20 s.
• Reaction: centrifuge decelerates in a  The drive is overheated. Switch off the
non-braked manner. centrifuge and allow it to cool down.
• 1: No standby after switching on. Start the centrifuge again after > 15 min.
• 2: Error on PCB converter (fault during  Check the mains voltage! If the voltage
braking). fluctuates, are other devices overloading
• 4: PCB converter has performed a reset. the power lead?
• 5: Drive motor overheating  Evaluate the error messages (customer
service function 45: FUA/FUK = last
• 6: Connections of drive motor and
error on the PCB converter drive).
compressor reversed
• 7: Overcurrent during braking (brake  Check/replace the reed switch.
resistor defective)  Check/replace the brake resistor (160
• Error (FUA/FUK) on the PCB converter. ohm).
Causes:  Check the motor for free movement.
1: Overcurrent (IPEAK). Exchange the motor.

2: Overvoltage brakes.  Replace the PCB converter.

3: Undervoltage in DC link.
4: Excess temperature - module.
5: Overcurrent (IEFF).
6: Timeout.
7: Internal converter error.

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5 Troubleshooting

Symptom/message Cause Remedy

Error 7 • Cut-off due to overspeed.  Wait for the rotor to stop. Tighten the
• 1: Maximum speed of rotor has been rotor.
exceeded.  Test the rotor detection and rotor
• 2: Deviations from target speed > 800 rpm impulses (service function 3).
 Check/replace the motor cap with
integrated rotor sensor (customer
service function 42: nR = rotor speed).
 Check the motor for free movement.
Exchange the motor.
 Replace the PCB converter.
Error 8 • Acceleration ramps are not observed or a
rotational speed measuring system is
defective.
• Reaction: centrifuge brakes.
• 1: Ramp control during acceleration (time
> 40 s).
• 2: Difference between motor rotational
speed and rotor rotational speed too high.
• The rotor is not mounted or the motor
rotational speed is missing.

Error 9 • EEPROM write error.  Switch off the centrifuge. Switch the
centrifuge back on after > 20 s.
 Initialize the EEPROM (service function
5).
 Perform a software update.
 Replace the PCB converter.
Error 10 • EEPROM read error.
• Reaction: error displayed and the standard
settings set. If errors occur when the
imbalance setting is read, the centrifuge
can no longer be started (see Error 2).

Error 14 • EPROM check sum error after mains  Switch off the centrifuge. Switch the
voltage has been switched on centrifuge back on after > 20 s.
• Error in the program memory, EPROM  Perform a software update.
defective.
 Replace the front panel with PCB
• Reaction: no further actions possible. controller.

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5 Troubleshooting

Symptom/message Cause Remedy

Error 15 • Impermissible imbalance.  Load the rotor symmetrically.

• Centrifuge was pushed.  Test the centrifuge with a different rotor
• Table not stable. and rotor lid.
• Rotor not tightened.  Check the motor suspension and shock
• Reaction: centrifuge stops run and mounts.
decelerates in a braked manner.  Check/exchange the PCB speed/
imbalance.
 Check the sensor idle value. (Customer
service function 44: US = sensor idle
value (see p. 35)).
 Check the imbalance detection (service
function 13).

Error 16 • Error when polling PCB converter.  Switch off the centrifuge. Switch the
• 2: Error - repeating of telegram centrifuge back on after > 20 s.
• Reaction: centrifuge switches off and  Replace the PCB converter.
decelerates in a non-braked manner.

Error 17 • Error during transmission of select telegram  Switch off the centrifuge. Switch the
to frequency converter. centrifuge back on after > 20 s.
• 1: Overflow of queue for PCB converter  Check the motor excess temperature
telegrams sensor. Check the connections between
• 2: Error after a select telegram has been the motor and the PCB converter.
repeated three times. Reaction: centrifuge  Exchange the motor.
switches off and decelerates in a
non-braked manner.  Replace the PCB converter.
• Reaction: centrifuge switches off and
decelerates in a non-braked manner.

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5 Troubleshooting

Symptom/message Cause Remedy

Error 18 • 1. After adjustment (Temp), interval of +/-  Let the centrifuge cool down. Repeat the
5°C exceeded. run.
• 2. After run started, temperature in rotor  Wait for 4 hours after transportation. The
chamber > set temperature. centrifuge should have room
During the run, the temperature in the rotor temperature 23°C.
chamber increases to the set temperature +  Is the room temperature too high? Is
16°C. there direct sunlight?
• 3. Temperature in rotor chamber > 50°C.
 Is there sufficient space for circulation of
• Reaction: centrifuge switches off and air around the sides of device? Are the
decelerates in a non-braked manner. The air slots free of obstruction?
compressor switches off.
 Is the lid sealing damaged; the lid closed
properly? (The entrance of air may
cause severe icing in the rotor chamber
and the control may be affected so that
the full power is not reached)
 Is the condenser fan contaminated,
defective? Check using the service
function.
 Check/replace the condenser
temperature sensor and the condenser
on the bottom of the rotor chamber.
 Check all plug connections.
 Replace the condenser (compressor).
 Replace the PCB converter.
Error 19 • Overheating at condenser > 65°C.  Let the centrifuge cool down. Repeat the
• Condenser fan does not start. run.
• Temperature sensor of condenser  See error 18.
defective.
• Reaction: centrifuge switches off and
decelerates in a braked manner; the
compressor is switched off.

Error 20 • Temperature sensor on bottom of rotor  Let the centrifuge cool down. Repeat the
chamber defective. run.
• Reaction: centrifuge switches off and  Customer service function 40: check/
decelerates in a braked manner; the replace the temperature sensor on the
compressor is switched off. bottom of the rotor chamber.
 Replace the PCB converter.
Error 21 • Temperature sensor of condenser  Let the centrifuge cool down. Repeat the
defective. run.
• Measured value outside -10°C.......+80°C.  Customer service function 40: check/
• Temperature of rotor chamber > 50°C. replace the condenser temperature
• Reaction: centrifuge switches off and sensor.
decelerates in a braked manner; the  Replace the PCB converter.
compressor continues to run.

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5 Troubleshooting

Symptom/message Cause Remedy

Error 22 • 50/60 Hz signals for fan control missing.  Let the centrifuge cool down. Repeat the
• Measured power frequency fluctuates run.
significantly.  Eliminate interference in the vicinity. Test
• Reaction: fan is activated at full power. the centrifuge in another room with a
different power supply circuit.
 Replace the PCB converter.
Error 24 1. Condenser overheated. Compressor error  Let the centrifuge cool down. Repeat the
on PCB converter cannot be deleted. run.
2. Error on PCB converter  Check the mains voltage! (Do any
3. Condenser (compressor) overheated and fluctuations occur when the rotor or the
sluggish compressor start? Is the power supply
overloaded by other devices?)
Cause of error on the PCB converter (FUA/  Is there sufficient space for circulation of
FUK): air around the sides of device? Are the
1. Overcurrent (IPEAK). air slots free of obstruction?
2. Overvoltage during braking.  Check using the service function.
3. Undervoltage in the intermediate circuit.  See Error 18.
4. Excess temperature on the PCB converter.
5. Overcurrent (IEFF).
6. Timeout.
7. Internal error in the converter

Reaction
• Centrifuge switches off and decelerates in a
braked manner, the condenser
(compressor) is switched off.

Error 25 • Mains/power failure during a run.  Check the supply voltage. If the voltage
• Undervoltage or overvoltage fluctuates, are other devices overloading
the power lead?
• PCB converter defective.
 Eliminate interference in the
environment. Test the centrifuge in
another room with a different power
supply circuit.
 Replace the PCB converter.
Error 26 • Disruption of communication with control  Switch off the centrifuge. Switch the
computer. centrifuge back on after > 20 s.
• Data transfer for operation.  Repeat the software update with
• Interference during software update with CefuProgrammer.
CefuProgrammer. Interruption during  Replace the cable between the
CefuComTest. PCB controller and the PCB converter.
 Replace the front panel with PCB
controller.
 Replace the PCB converter.

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5 Troubleshooting

Symptom/message Cause Remedy

Error 27 • Disruption of communication with control  Switch off the centrifuge. Switch the
computer. centrifuge back on after > 20 s.
• Interference during software update with  Repeat the software update with
CefuProgrammer. Interruption during CefuProgrammer.
CefuComTest.
 Replace the front panel cable.
• Reading data from control computer – keys
not detected.  Replace the front panel with PCB
controller.
• Reaction: centrifuge stops run and
decelerates in a braked manner.  Check the PCB converter and replace it
if necessary.

Error 28 • Electronics fault.  Open lid: Press open button.

 Replace the front panel with PCB
controller.

Error 29 • Target speed > maximum speed of inserted  Rotor detection: check g-force after rotor
rotor. replacement.
• Reaction: centrifuge stops run after rotor  Adjust the target speed to the rotor or
detection. use a different rotor.
 Start the centrifuge again.
Error 30 • Lid cannot be opened or closed. Troubleshooting guide (see Troubleshooting
• 1 (11): Lid could not be latched, limit switch Error 30: Lid cannot be opened or closed on
not reached => lid opens again. p. 28).
• 2 (12): Lid could not be opened, at least
one limit switch is still closed.
• 3 (13): Lid closed at standstill, open a limit
switch again => lid opens again.
• 4 (14): Error when opening lid, at least one
limit switch is still closed.

5.2 Error messages from service programs/other error messages

Symptom/message Cause Remedy

Error 36 • EEPROM not yet initialized.  Initialize the EEPROM, (see Service
function 5: Initialization on p. 40).
 Replace the PCB converter.
Error 38 • Error during plant inspection.  Only for plant inspections: Error when
using a service function.

Error 39 • Error during plant inspection.  Only for plant inspections: Error when
using a service function.

Possible that no • Electronics fault.  Replace the front panel with PCB
display/keys blink. controller.
 Replace the PCB converter.

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5 Troubleshooting

5.3 Troubleshooting Error 30: Lid cannot be opened or closed

Description of the latch operation (see Lid latch functionality on p. 69).

If the lid cannot be opened or closed, the message "Lid latch note A: The Lid could not be
Hint! latched. Close lid again" will be shown twice in the display.
If the error occurs a third time, the display shows "Error 30".

Tab. 5-1: Possible causes for Error 30

Code for possible cause Lid position Limit switch position

Error 30 Note A lid latch

1 11 Lid could not be latched. Limit switch not reached. Lid opens again.

2 12 Lid could not be unlatched. At least one limit switch is still closed.

3 13 Lid was closed in standstill. One limit switch open. Lid opens again.

4 14 Error when opening the lid. At least one limit switch is still closed.

Tab. 5-2: Troubleshooting procedure

1. Switch off the centrifuge. Switch the centrifuge back on after 20 s.

Determine the cause and frequency of the error

2. Call customer service function 45, (see 45: Error messages on p. 35).
The last 4 errors are displayed. Check the possible causes of the error, (see Tab. on p. 28).

3. Call service function 8, (see Service function 8: Error messages and data from the last 4 runs on p. 42).
The last 30 errors are displayed. Check how often "Error 30" occurred.

Check the stress on the lid

4. Call service function 9, (see Service function 9: Operating hours and number of load changes/cycles on p. 42).
The number of all centrifuge load changes is displayed. This number indicates how often the centrifuge lid was
opened and closed. The centrifuge lid is subject to wear.

Clean and grease the lid latch unit (see Maintenance services for centrifuges on p. 81).

5. Clean the spindle and thread of the lid latch nut with degreasing agents, e.g., alcohol. Do not use acidic
substances.

6. Grease the lid latch unit with gleitmo WSP 5040 high-performance grease.

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5 Troubleshooting

Test lid switch

7. Call customer service function 43 (see 43: Rotor detection and lid latch on p. 34).
0000I1111 0=open
1=closed

Reedswitch
Switch for lid position
Micro switch for end position right
Micro switch for end position left
Display only
for plant inspections

8. Check to see which switches have switched.

The reed switch is activated first. The lid position switch then starts the two latch motors. Lastly, the two limit
switches switch off the latch motors.

9. If the latch motors do not start, check the reed switch and the magnet for the lid position (see Magnet for lid
position (actuator) on p. 50).

10. Call service function 2 (see Service function 2: Test the lid function on p. 39).

10. Perform the lid function test.

11. Check the non-functional lid switch, adjust and replace it if necessary (see Adjust the lid latch switch on p. 69).

Check the microswitch for the lid position

12. Switch the device off. Disconnect the power plug.

13. Remove the front panel with the PCB operating of the centrifuge.

14. Check the condition and position of the microswitch.

15. Adjust the microswitch for the lid position if necessary (see Setting the microswitch for the lid position on p. 70) or
replace it (see Lid latch unit on p. 60).

Check the current consumption of the latch motors

16. Call customer service function 43 (see 43: Rotor detection and lid latch on p. 34).

17. Check the calculated values of the current consumption P1 and P2. The smaller the characteristic value, the better
the current consumption of the latch motors.
Characteristic values < 35 are typical for P1 and P2.

18. If the characteristic values > 35, check the lid mechanics and the condition and the adjustment of the latch motors.

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5 Troubleshooting

Check the lid mechanics, hinges and lid latch

19. Check the condition and position of the lid.

The lid is higher on the left side due to the one-sided gas spring.
The lid must close correctly and may not touch the front panel when closing.
The lid must not be warped.
The lid must be aligned with the lid latch unit and the housing.

20. Press against the springs of the lid latch nuts.

The lid latch nuts must move freely in a vertical direction.
The lid latch nut must be aligned with the lid latch unit in the device.
If a lid latch nut is broken in the lid, use new lid latch nuts (see Lid latch nut on p. 49).

21. Check the hinges.

22. If the hinges are warped or askew, replace the housing (see Upper part of housing on p. 67).
If the rivet joints are loose between the housing and hinge or have too much clearance, replace the housing
(see Upper part of housing on p. 67).

Check the lid latch unit mechanics.

23. The screws must be firmly tightened and the mechanics must be able to move.
Inspect the thread of the lid latch nut for wear.
If there are signs of wear or damage, replace the lid latch unit with lid nut (see Lid latch unit on p. 60).

24. The switches of the lid latch must be adjusted after the lid or the lid latch has been replaced (see Adjust the lid
latch switch on p. 69).

Check the electronics

25. Check the PCB converter plug connections.

26. Check the PCB converter and replace it if necessary, (see PCB converter 5430 R on p. 54).

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5 Troubleshooting

5.4 Customer service functions

5.4.1 Overview

Number Description of function

40 Temperature and cooling

41 PCB converter cooling

42 PCB converter drive

43 Rotor detection and lid latch

44 Imbalance detection

45 Error messages

46 General device information

5.4.2 Activation and operation

The customer service functions can be activated in each operating state. without affecting the
Hint! centrifuge run.
When an error occurs, the display switches to customer service function 45 (see 45: Error messages on p. 35).
For troubleshooting, only use the bold functions, e.g., motor rotational speed "nM". All other entries are reserved for plant
inspections only.

Activate

• Press the and keys simultaneously for longer than 2 s.

Operate

• Use the and keys to select the customer service function.

Exit

• Press the and keys simultaneously for longer than 2 s.

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5 Troubleshooting

5.4.3 40: Temperature and cooling

P3=

TX: Temperature of aspirating tube

KA: Compressor status (0 = OFF; 1 = ON)

LS:: Fan level

TKE: Temperature of the rotor bowl

TS: Set temperature

TKO: Condenser temperature

P1/P2/P3 Additional information

5.4.4 41: PCB converter - cooling

41 nKO= 4300 nmax= 6000 UZK= 380

IKO= 4.3 TM_KO= 50 Err= 0
STAT= 2B5A P1= P2=
10:00 min 10 °C 5000 rpm

nKO: Compressor speed

nmax: Max. permitted compressor speed

UZK: Intermediate circuit voltage

IKO: Compressor current I-eff

TM_KO: Compressor temperature

Err: Last error from the compressor

STAT: Compressor status display (HEX)

P1/P2 Additional information

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5 Troubleshooting

5.4.5 42: PCB converter - drive

42 nM= 11001 nR= 11000 DF= 1153

IZK= 0.8 UZK= 387 TM= 58 TP= 0
STAT= 0660 U0= 0 UN= 170 DC= 1200
10:00 min 10 °C 5000 rpm

nM: Motor rotational speed (PCB speed/imbalance)

nR: Rotor rotational speed (motor cap)

DF: Rotating field (PCB converter rotational speed adjustment)

IZK: Operation current (current for the intermediate circuit)

UZK: Intermediate circuit voltage

TM: Temperature of the module (drive)

TP: Temperature of the PFC

STAT: Drive status display (HEX)

U0: Acceleration voltage

UN: Nominal voltage

DC: Intensity direct current brake

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5 Troubleshooting

5.4.6 43: Rotor detection and lid latch

RR: Rotor relation See chapter rotor table

No: Number of measurements

Rn: Rotor number See chapter rotor table

IDV: Maximum current - lid latch Only for plant inspections.

ADV: Cut-off value - lid latch Only for plant inspections.

DR
0000I1111 0=open
1=closed

Reedswitch
Switch for lid position
Micro switch for end position right
Micro switch for end position left
Display only
for plant inspections

AM: Derive mode - lid latch Only for plant inspections.

MS: Machine status

P1: Calculated characteristic value of the current Typical characteristic value < 35
consumption for the left latch motor

P2: Calculated characteristic value of the current Typical characteristic value < 35
consumption for the right latch motor

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5 Troubleshooting

5.4.7 44: Imbalance detection

US: Sensor idle value (visible during the centrifuge standstill, sensor idle value limits: 300 to 700)

UR: 0

UA: 0

HR: Acceleration ramp

BR: Braking ramp

CB: Check byte

P1/P2/P3: Additional information

5.4.8 45: Error messages

Top row:
Error: Last error that occurred FUA: Error on the PCB converter drive FUK: Error on the PCB converter
compressor (only active for Refrigerated
Microcentrifuge 5430R)
Middle and bottom row:
1: Last error that occurred 2: Second to last error
3: Third to last error 4: Fourth to last error

Example: The third to last error is error 6.

3: 6 2 4

Error 6 Cause 2 Cause 4

2: Error on the PCB converter, fault 4: Excessive temperature in a
during braking module on the PCB converter

Error messages and their causes (see Error messages on p. 21) .

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5 Troubleshooting

5.4.9 46: General device information

Type: Internal device number

xxx V Voltage

-- Hz Frequency

V_C: Software version of the PCB converter control unit (update: mainboard software); will be shown in the
display after the centrifuge has been switched on.

CS_C: Check sum of the mainboard software (HEX)

V_D: Display software version on the PCB controller

aCRC: Check sum of the display software (HEX)

V_F: Software version of the PCB converter drive (update: mainboard software, see V_C:)

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5 Troubleshooting

5.5 Service functions

5.5.1 Overview

Number Functions

1 Display test and key test

2 Test the lid function

3 Test rotor detection and rotor impulses

4 Determine acceleration times and deceleration times

5 Initialization

6 Test overspeed cut-off

7 Endurance test

8 Error messages and data from last 4 runs

9 Operating hours and number of load changes/cycles

10 Check USB interface using CefuComTest

11 Test individual units (fan/compressor)

12 Run with adjustable constant compressor frequency

13 Check imbalance detection

14 Machine test (only for plant inspections)

5.5.2 Activation and operation

The service functions run separately from normal centrifuge operation.

Activate
• Switch off centrifuge.

• Press the and keys simultaneously, and switch on the mains power switch in the process.

Operate

• Use the and keys to select the service function.

• Use the menu key or the arrow key to start the service function.

Exit
• Centrifuge switched off.

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5 Troubleshooting

5.5.3 Service function 1: Display test and key test

START Program Key display

Press the START key to start the service function. Press any key to continue.

Display test:
• The program LEDs and the OPEN LED light up blue. The standby LED lights light green.
• The display shows six chessboard patterns, which are magnified every time a key is pressed.

LED test:
• The program LEDs and the OPEN LED light up blue. The standby LED lights light green.
• The standby LED lights red after additional keystrokes.
• After another keystroke, the OPEN LED lights blue and the standby LED lights light green. The PROG 1-5 keys are
activated successively.

Test the backlight, real-time clock and buzzer:

• The backlight does not light; the display shows: "Backlight off".
• The backlight lights; the display shows: "Backlight on".
• The current time of the real-time clock is shown in the display in the format 12:00:00. The clock continues to display
the time in seconds. You will hear 3 tones of the buzzer.

Key test:
• Press all keys and rotary knobs one after the other. The real-time clock will be displayed.
• When all keys and rotary knobs were recognized, the display will show 255 255 63.

• Press menu left two times.

• When all keys were pressed and recognized, the display will show: "Test completed". The program is ended.
• If not all keys were pressed or recognized, the display will show: "Test failed. Not all keys are pressed!" The test
program is ended with the next keystroke.

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5 Troubleshooting

5.5.4 Service function 2: Test the lid function

START Program Lid latch

0000I1111 0=open
1=closed

Reedswitch
Switch for lid position
Micro switch for end position right
Micro switch for end position left
Display only
for plant inspections

Key: Function

Open Open. Locking independent from the position.

Close. Locking up to limit switch.

MENU CURSOR

RPM/RCF Close the locking mechanism step by step until the limit switch has
been reached and opens.

SPEED_PLUS Unlock the right-hand side.

Turn the SPEED rotary knob to the right

SPEED_MINUS Lock the right-hand side step by step.

Turn the SPEED rotary knob to the left

TIME_PLUS Unlock the left-hand side.

Turn the TIME rotary knob to the right

TIME_MINUS Lock the left-hand side step by step.

Turn the TIME rotary knob to the left

SHORT Lid latch endurance test. Close the lid by hand.

The fans for the motor and the converter are switched on.

START/STOP Leave the endurance test.

End the test program.

MENU

Cut-off: 1 Switch off the lid latch motors over the time period.

2 Switch-off when the right end limit is reached. The last pressed limit switch, i.e. 2 or 5, is displayed.

3 Switch-off due to the overcurrent on each motor

4 Switch-off due to total overcurrent

5 Switch-off when the left end limit is reached The last pressed limit switch, i.e. 2 or 5, is displayed.

6 Overcurrent motor (left)

7 Overcurrent total

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5 Troubleshooting

5.5.5 Service function 3: Test rotor detection and rotor impulses

START Program Rotor detection

 Slowly rotate the rotor by hand.

The magnet sequence is shown in the display.

RR Rotor relation, see rotor table (see p. 45)

RN Rotor number, see rotor table (see p. 45)

DL Number of measurement procedures

5.5.6 Service function 4: Determine acceleration times and deceleration times

START Program Accel./braking time

The specifications for the centrifuge acceleration and braking times can be found in the Technical Data chapter in the
service manual.
1. Load the rotor completely.
2. Close the centrifuge lid.
3. Start the measurement with the START key.
The centrifuge accelerates to the maximum rotor speed.

5.5.7 Service function 5: Initialization

The target values and operating states are stored on the EEPROM after each centrifuge run. Statistical data, the data from
the last runs, the load change per rotor and error messages are also stored on the EEPROM.

Deleted on initialization Not deleted on initialization

Error messages Language setting

Operating hours (h) Programmed user programs

Load change (CL) total

Load change per rotor

START Program Initialization

Key: MENU/ENTER Regular initialization <Emphasis/>

Key: SHORT First initialization after initial setup – setting in English.

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5 Troubleshooting

5.5.8 Service function 6: Test the overspeed cut-off

Damage to device caused by excessive speed.

The centrifuge should automatically switch off when the cut-off speed of the inserted rotor has
been reached.
NOTICE!
 Find out more information about the maximum speed of the inserted rotor.
 Observe the test.
 If the centrifuge does not automatically switch off, disconnect the centrifuge from the mains
power supply.

The rotor overspeed cut-off is 300 rpm more than the maximum speed.
For this test, the centrifuge accelerates up to 500 rpm above the maximum speed of the rotor.
Hint!

START Program Overspeed

1. Define the maximum permissible speed of the rotor.

2. Insert the rotor and close the lid.
3. Start the test with the START/STOP key.
The centrifuge accelerates. The display shows Error 7 when the cut-off speed has been reached. The centrifuge
automatically switches off and decelerates in a braked manner.
If the centrifuge does not automatically switch off, disconnect the centrifuge from the mains power supply.

5.5.9 Service function 7: Endurance test

START Program Run cycle

The SHORT, SOFT and FC functions are not available during the test.
1. Insert the rotor.
2. Rotate the rotor by hand.
Program 1 or program 2 will be selected based on the inserted rotor.

3. Select program 3 using the key. Carry out settings for program 3.
4. Start the test with the START/STOP key.
The centrifuge accelerates. The test will be executed with the maximum rotor speed.
5. End the test during a program break using the START/STOP key.

Program Rotor Cycle for Centrifuge 5430 Cycle for Centrifuge 5430 R

1 Fixed-angle rotors 30 runs of 10 min, with an interval of 50 runs of 10 min, with an interval of
5 min between each run 5 min between each run

2 Swing-bucket rotors 20 runs of 2 min, with an interval of 36 runs of 2 min, with an interval of
2 min between each run 2 min between each run

3 Free selection 30 runs of 5 min, with an interval of 2 min between each run

Set the run time and the length of the interval before the start. Use the key to
set the interval length from 1 min to 20 min.

Each cycle is followed by an interval of 30 min.

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5 Troubleshooting

5.5.10 Service function 8: Error messages and data from the last 4 runs
The first error of each run will be stored separately for the centrifuge and the frequency converter.

START Program Table of errors

Switch the display further with the START key

Error messages for centrifuge 1-15
Error messages for centrifuge 16-30

Error messages drive (PCB converter: drive)

Error messages cool (PCB converter: compressor)

The last 4 error messages

Data of the last runs

5.5.11 Service function 9: Operating hours and number of load changes/cycles

START Program Load change

Display: Load change/number of cycles (CL) total

Operating hours (h)

Switch the display further with the START key

Display: Load change/number of cycles (runs) for each rotor

If you have replaced the PCB controller, transmit the load change/number of cycles (runs) for
Hint! each rotor from the old PCB.
• RPM/RCF key= delete the counter
• TIME-PLUS key = increase counter by 500 (up to max. 98,000)
• TIME-MINUS key = decrease counter by 500

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5 Troubleshooting

5.5.12 Service function 10: Check USB interface using CefuComTest

A detailed description of the CefuComTest can be found on the website www.eppendorf-support.com.

START Program Interface

Prerequisites
• USB-A to USB-B connection cable, order no. 5428 850.280
• PC/laptop
1. Download the CefuComTest program and the software instructions from the website www.eppendorf-support.com .
2. Perform the CefuComTest program according to the software instructions.

5.5.13 Service function 11: Test individual units (fan/compressor)

Close the centrifuge lid. The compressor is released.

START Program Cooling temperature/fan

Key: START/STOP Fan for motor 0 = off

1 = slow setting

2 = fast setting

SHORT Fan for converter 0 = off

1 = slow setting

2 = fast setting

SPEED_PLUS Condenser fan on/off

TIME_PLUS Increase condenser fan setting 0 to 32

TIME_MINUS Decrease condenser fan setting 32 to 0

TEMP_PLUS Compressor on/off

End the test program

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5 Troubleshooting

5.5.14 Service function 12: Run with adjustable constant compressor frequency

START Program Compressor frequency

For plant inspection only.

5.5.15 Service function 13: Check imbalance detection

START Program Imbalance test run

During the run, the following values are shown on the display:
• US = current imbalance value
• UR = maximum imbalance that occurred in the current run
• UA = imbalance cut-off value, changes dependent on the current speed

Perform the test using all available rotors.

Hint!

5.5.15.1 Preparing test weights

An imbalance compensation is not required.
Test weights for rotors (see Rotor table and imbalance cut-off weights for Centrifuge 5430/5430R on p. 45).

G1 G2 G3

With this weight, the centrifuge must Programmed cut-off speed. With this With this weight, the imbalance
run without errors. The imbalance weight, the imbalance detection can detection must switch off the
detection must not switch off the switch off the centrifuge. centrifuge.
centrifuge.
Due to manufacturing tolerances the
devices respond differently.

 Prepare test weights G1 and G3 for the rotor to be tested.

 Use a filled and sealed tube as the test weight.
 Consider the empty weight of the tube with lid.
 The tube must fit into the rotor to be tested.
 If the test weight cannot be reached with a single tube, put several tubes in adjacent bores in the rotor.

5.5.16 Service function 14: Machine test

Only for plant inspections.

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5 Troubleshooting

5.6 Rotor table and imbalance cut-off weights for Centrifuge 5430/5430R
Checking imbalance detection (see Service function 13: Check imbalance detection on p. 44) .

Rotor number Rotor designation Speed/RCF Rotor relation G1 G2 G3

(RN) (RR)
Radius

2 A-2-MTP 4680 rpm/2204 × g 153.9 10 g 20 g 30 g

90 mm 139 ... 167

5 F-35-6-30 7830 rpm/7197 × g 269.2 3.5 g 7g 15 g

105 mm 255 ... 283

8 F-45-18-17-Cryo 8900 rpm / 8320 × g 384.6 3.5 g 7g 10 g

94 mm 371 ... 399

10 F-45-64-5-PCR 11800 rpm / 13543 × g 461.5 2g 4g 10 g

87 mm 447 ... 475

12 S-24-11 12700 rpm 16049 × g 538.5 1.85 g 3.7 g 7g

89 mm 524 … 552

13 FA-45-24-11-KIT 13200 rpm / 19090 × g 577 1.75 g 3.5 g 7g

98 mm 563 ... 591

14 F-45-30-11 14000 rpm/20817 × g 615.4 1.75 g 3.5 g 7g

95 mm 601 ... 629

15 FA-45-30-11 14000 rpm/20817 × g 654 1.5 g 3g 7g

95 mm 640 ... 668

16 FA-45-16-17 14200 rpm 21191 × g 692.3 3g 1.5 g 7g

94 mm 678 ... 706

18 FA-45-24-11-HS 17500 rpm / 30130 × g 769.2 1.25 2.5 g 7g

88 mm 755 ... 783

30 F-45-48-11 12700 rpm 17083 × g 500 4g 2g 7g

101 mm 486 ... 514

31 FA-45-48-11 12700 rpm 17083 × g 538,5 4g 2g 7g

101 mm 524 ... 552

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5 Troubleshooting

5.7 Fuses
Instead of removable fuses, the Centrifuge 5430 R features a thermal overcurrent protective switch. The mains power
switch jumps to the '0' switch setting if the overcurrent protective switch is triggered.
1. Use the mains power switch to switch the device back on after more than 20 s.
2. If the mains power switch returns to the '0' switch position, correct the problem in the device or replace the defective
mains power switch.

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6 Disassembly/assembly
6 Disassembly/assembly
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

6.1 Tools and auxiliary equipment

Tools required
1. Phillips screwdriver size 2
2. Phillips screwdriver size 0
3. Allen key (Inbus) 1.5 mm
4. Allen key (Inbus) 2.5 mm
5. Open-end wrench WAF=7 mm
6. Socket wrench 13 mm or box/open-end wrench
7. Socket wrench 7 mm or pipe wrench
8. Socket wrench tool kit with small extension WAF=7 mm or flexible short socket wrench
9. Watchmaker screwdriver 1-1.5 mm
10. Side-cutting pliers
11. Flat pliers
12. Rotor key, order no. 5416 301.001
13. Housing service key, order no. 5428 850.620

Recommended lubricants and adhesives

• Heat-conducting paste
• Loctite 5910
• Silicone rubber E 41

Measuring devices required:

• Multimeter or ohmmeter

Assembly is performed in reverse order to disassembly. If an adjustment is necessary, this is

Hint! indicated.

After the lid and the lid latch have been removed or replaced, the limit switches must be adjusted,
Hint! (see Adjusting the right and left limit switches on p. 71).

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6 Disassembly/assembly

6.2 Upper lid part and monitoring glass

6.2.1 Remove the upper lid part

Prerequisites
None

1. Open the centrifuge lid.

The lid can be opened using the emergency release, see
OM. If that is not possible, (see Opening the centrifuge
lid if the emergency release is defective on p. 68).
2. Unscrew the four Allen keys (2.5 mm) (1) in the lid.
3. Close the lid with the emergency release. Turn by
approx. 2 revolutions on each side. It is easier to remove
1 1 a firmly-fixed upper lid part.
4. Lift off the upper lid part.

The centrifuge lids from serial number 6,609 upwards and the spare parts have been tested for
Hint! durability. Crack formation on the rear should be excluded in the long term. The checked lids
have the label "Replaced Lid" on the rear.
• Inspect all lids without a label for hairline cracks.
• Any complaints concerning lids with visible hairline cracks should be made via the complaints
procedure.

6.2.2 Remove the monitoring glass

Prerequisites
The upper lid part has been removed (see p. 48).
 Remove the monitoring glass along with the O-ring from the metal lid.

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6 Disassembly/assembly

6.3 Lid axle and lid panel

6.3.1 Remove the lid axle and the lid panel

Prerequisites
The upper lid part has been removed (see p. 48).
1. Remove the locking ring (1).
2. Pull out the lid axle (2) sideways.
3. Remove the lid panel.

6.4 Lid latch nut

Prerequisites
The upper part of lid has been removed (see p. 48).
1. Unscrew both M4 nuts (2).
2. Remove both tooth lock washers.
3. Remove both washers.
4. Remove the lid latch nut (1).
5. Remove both flat washers. They are in the metal lid with
the rounded side downwards.

The lid latch nut must be aligned with the lid latch unit in the device.
The lid latch nut must move freely in a vertical direction when pressure is applied to the springs.
Hint!

Check the setting of the microswitches of the lid latch units.

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6 Disassembly/assembly

6.5 Magnet for lid position (actuator)

6.5.1 Remove the magnet for lid position

Prerequisites
The upper part of lid has been removed (see p. 48).

1. To remove the magnet for lid position (actuator) (3),

remove the two M4 nuts(4).

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6 Disassembly/assembly

6.6 Gas spring

6.6.1 Remove the gas spring

Prerequisites
None
1. Open the centrifuge lid completely.
2. Remove the circlip (2) and push out the holding bolt (3).
23 3. Push the centrifuge towards the table edge or tilt it onto
the right side. Protect the mains power switch and cold
5 appliance plug.
4. Unscrew the 3 Phillips screws (1) under the device from
the base plate.
6 5. Pull out the gas spring (5) completely. If necessary,
slightly turn the gas spring to find the opening in the
ground plate.

When pulling out the gas spring, pay particular attention to the damping washer (6). It can easily
Hint! fall into the device.

6.6.2 Install the gas spring

1. The straight edge of the gas spring unit faces towards

the outside of the device.
2. Position the gas spring in the center and slide the
8 damping washer under the housing latch.
3. Press the screwdriver (7) under the holding loop of the
metal lid (8) and press the gas spring (5) down.
7 4. Slide the holding bolt from the right, if necessary, jolt
slightly to find the openings.
5 5. Mount the circlip by pressing it into the keyseat.

Always insert the holding bolt from the right.

Hint!

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6 Disassembly/assembly

6.7 Front panel with PCB controller 5430 R

6.7.1 Remove the front panel

Prerequisites
None

1 1

1. Unscrew the 2 Phillips screws (1).

2. Pull the front panel up slightly and place it onto the surface of the table.
3. Disconnect the plug connection to the PCB controller 5430 R (2) (note the engagement hook on the PCB converter
5430 R).
4. Remove the front panel complete with the PCB controller 5430 R.

The PCB controller 5430 R must not be unscrewed from the front panel.
Hint!

Before installing the front panel with the PCB controller, check the software version. Update the
Hint! software if necessary.

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6 Disassembly/assembly

6.8 Console and emergency release guide

6.8.1 Remove the console

Prerequisites
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The PCB Converter 5430 R has been removed (see p. 54).

The console is connected with the housing and the base plate with 8 Phillips screws.
Hint!

4x 4x

1. Unscrew the eight Phillips screws and remove the console to the front.

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6 Disassembly/assembly

6.8.2 Remove the emergency release guide

Prerequisites
• The front panel has been removed, (see p. 67).
• The PCB Converter 5430 R has been removed (see p. 54).
• The console has been removed(see p. 53).

1. Unscrew the countersunk screws (1) and remove the

emergency release guide.

6.9 PCB converter 5430 R

Prerequisites
Front panel with PCB controller 5430 R has been removed (see p. 52).
1. Disconnect all the plug and clamp connections to the PCB Converter 5430 R.
2. Remove the 2 Torx screws for the ferrite core fixing. Remove the cable.
3. Unscrew the 6 fastening screws of the PCB converter 5430 R. Remove the grounding cable.
4. Remove the PCB converter 5430 R to the front.

During installation, loosely screw the PCB with 2 screws and then establish the cable
Hint! connections.

The Pertinax plate between the heat sinks on the PCB converter 5430 R is used for air circulation
Hint! and must not be removed.

For the configuration of the plug and clamp connections, refer to the layout plan in chapter 8.
Hint!

Before installing the PCB converter, check the software version. Update the software if
Hint! necessary.

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6 Disassembly/assembly
1
12 6 9 10
11

3
4
2

8
13

16

17
14
15

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6 Disassembly/assembly

Plug Cable colors

1 Mains input X204/ X203 White/black

2 Drive motor X2 black/green/white

3 Motor temperature switch X195 gray/gray

4 Brake resistor X21 white/white

5 Compressor X3 black/blue/brown

6 Latch motor (right) X7 red/white

7 Imbalance/motor rotational speed X10 from left: red/gray/gray/gray according to the bundled
cable

8 Condenser fan X5 Plug white/white

9 Rotor rotational speed motor cap and X101 Plug from the right:
temperature sensor of bottom of chamber
Not assigned
Motor rotational speed sensor: brown/green/white
Temperature sensor (bottom of chamber): red/red

10 signals lid latch X6 From the right:

Limit switch (right): red/red
Switch for lid position: white/white
Limit switch (left): black/black

11 Latch motor (left) X72 Red/blue

12 Reed switch X9 black/black

13 Temperature sensor of condenser fan X11 gray-black/gray

14 Fan for motor X22 black/red

15 Fan for converter X4 black/red

16 PCB controller X1003

17 USB connection X15 not assigned/red/white/green/black/gray

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6 Disassembly/assembly

6.10 Mains power switch

6.10.1 Remove the mains power switch

Prerequisites
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The PCB Converter 5430 R has been removed (see p. 54).
• The console has been removed(see p. 53).
1. Press together the clamping of the mains power switch and press out of the housing.

You can insert a screwdriver through a bore in the base plate to facilitate its removal. Press
Hint! together the clamping with the screwdriver.
2. Disconnect the cable from the mains power switch.

For assembly, the mains power switch must be inserted in the housing so that the "0" is facing to
Hint! the front.

6.11 Brake resistor

6.11.1 Remove the brake resistor

Prerequisites
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The PCB Converter 5430 R has been removed (see p. 54).
• The console has been removed (see p. 53).
• The emergency release guide has been removed.
• The lid latch unit (left) has been removed.

1. Loosen the M8 fastening nut (1) with an open-end

wrench (WAF=13) at the top of the brake resistor (2).
2. Unscrew the screw from the underside of the device (3).
3. Remove the fastening nut and the lock washer upwards.
1
4. Remove the threaded rod from the lower part of the
4 housing and remove the resistor.
2

If the unsolder fuse (4) is actuated, the resistance is considered to be destroyed.

Do not solder in the spring-loaded soldering lug again!
Hint!

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6 Disassembly/assembly

6.11.2 Assembly of the brake resistor

1. Preassemble the threaded rod with counter nut and brake resistor (2).
2. Screw the threaded rod into the lower part of the housing and fasten it using a counter nut. The threaded rod must be
flush with the bottom!
3. Install the retaining washer and the fastening nut (1) at the top.
4. Align the resistor in such a way that the connections point away from the chamber at the side. Do not overtighten the
fastening nut (1) as the ceramic body might break.

6.12 PCB imbalance/motor rotational speed

Damage to the compressor and refrigerating system due to tilting the device.
The compressor and refrigerating system can become damaged if the device is switched on
when it is tilted or if the device is switched on right after it was tilted.
NOTICE!
 Leave the device in the normal position for at least 4 hours before switching it on.
 Never place the device upside down.

6.12.1 Remove the PCB imbalance/motor rotational speed

Prerequisites
The housing front panel with PCB operation 5430 R has been removed (see p. 52).
1. On the PCB converter 5430 R disconnect the ribbon
cable of the plug X 10.
2. Tilt the centrifuge onto the right side (protect the mains
switch and the cold appliance plug).
3. On the lower side of the motor, unscrew the two
assembly screws (1) of the PCB and disconnect the
2 cable fixing (2).

You do not need to remove the motor to exchange the PCB imbalance/motor rotational speed.
Hint!

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6 Disassembly/assembly

6.13 Fans for PCB converter 5430 R and motor

6.13.1 Remove the fans for the PCB converter 5430 R and the motor

Prerequisites
• The housing front panel with PCB operation 5430 R has been removed (see p. 52).
• The PCB converter 5430 R has been removed (see p. 54).
• The console has been removed(see p. 53).

1. Unscrew the front (1) and rear nuts with a wrench

WAF=7.
2. Pull the centrifuge to the edge of the table and remove
the screws downwards. Remove the nuts with flat
washers.
3. Remove the fan.

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 Centrifuge 5430 R — Service manual - English

6 Disassembly/assembly

6.14 Lid latch unit

Prerequisites
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The PCB Converter 5430 R has been removed (see p. 54).
• The console has been removed (see p. 53).
• The emergency release guide has been removed (see p. 54)

The lid latch unit can be replaced more easily when the housing is removed.
Hint!

The lid latch nut in the lid should also always be replaced.
Hint!

The lid latch nut in the lid must be aligned with the spindle of the lid latch unit.
Hint!

1. To remove the latch units, unscrew 2 nuts WAF=7 (1) in

each case. This requires a socket wrench tool kit with
flexible extension.

2. To assist with disassembly, guide an open-end wrench

WAF=7 (2) through the ventilation slots in the housing.

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 Centrifuge 5430 R — Service manual - English

6 Disassembly/assembly

An insulating mat is installed between the housing and the assembly plate of the latch unit.
Hint!

If the housing is not removed for disassembly, it is recommended that you tilt the device onto the
Hint! right side (protect the mains power switch and the cold appliance plug) in order to facilitate
assembly of the flat washers and nuts.
Abb. 6-1: Both lid latch units in installed condition.

Fig. 6-1: Both lid latch units in installed condition.

For adjustment of the microswitch (see p. 69)

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 Centrifuge 5430 R — Service manual - English

6 Disassembly/assembly

6.15 Motor cap with insulating parts

The motor cap with insulating parts includes the motor cap with the rotor sensor, the motor sleeve, the insulating cap and
the supporting ring for the motor sleeve.

6.15.1 Remove the motor cap with insulating parts

Prerequisites
• The rotor has been removed.
• Front panel with PCB Operating 5430 R has been removed (see p. 52).

1. Unscrew the 4 Phillips screws of the motor cap (4). Lift

the motor cap (4) including the speed sensor upwards
and place to the side. Remove any silicone rubber
residues.
2. Remove the motor sleeve (3). Remove any loctite and
silicone residues.

3
4

3. Remove the insulating cap (2).

4. Remove the supporting ring for the motor sleeve.
5. Remove any silicone residues from the rotor chamber if
necessary.
6. Loosen the cable on the X 101 plug of the PCB
Converter with a small watchmaker screwdriver. This
unlocks the latch mechanism.
7. Remove the motor cap.

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6 Disassembly/assembly

6.15.2 Install the motor cap with insulating parts

1. Place the supporting ring for the motor sleeve (1) with
the pin facing downwards on the motor. The recess for
the cable of the motor cap faces towards the temperature
sensor.
2. Insert the insulating cap (2) and the motor sleeve (3).
3. Fasten the motor cap with the 4 Phillips screws (5) and
remove the 4 Phillips screws again.
4. Thread the motor sleeve (3) in the edge of the rotor
chamber and apply "Loctite 5910" at 12 points around
the edge. Ensure the motor sleeve is positioned
centrally.
5. Apply "Silicone rubber E 41" as sealant under the motor
cap (4), in the area around the four screw holes along
the periphery of a ring.
6. Insert the 4 Phillips screws of the motor cap (4) and
2 5 1 3 tighten.
7. Connect the cable connections to the PCB Converter
5430 R.

6.16 Temperature sensor of bottom of chamber

6.16.1 Remove the temperature sensor

Prerequisites
• The rotor has been removed.
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The motor cap with insulating parts has been removed (see p. 62).
1. Loosen the cable to the PCB Converter 5430 R, X 101 plug. Unlock the latch mechanism using a watchmaker
screwdriver.
2. Press the temperature sensor from the rotor chamber downwards and remove it.

6.16.2 Install the temperature sensor

1. Insert the temperature sensor from above, with the cables to the front, into the opening and push in.

Installation dimension measured approx. 11 mm from the bottom of chamber.

Hint!

2. Connect the cable.

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6 Disassembly/assembly

6.17 Centrifuge motor and motor suspension

6.17.1 Loosen the cable connections on the PCB Converter

Requirements:
• The rotor has been removed.
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The motor cap with insulating parts has been removed (see p. 62).
Loosen the following cable connections:

Centrifuge motor X2 plug black, green, white

Motor temperature switch X195 plug gray, gray

Imbalance/motor rotational speed X10 plug red, gray, gray, gray

6.17.2 Remove the centrifuge motor and motor suspension

1. To remove the motor, unscrew the 3 M8 (1) nuts.

2. Remove the flat washers and curved spring washers.
3. Lift the motor and place it in such a way that the
grounding wire is visible. Unscrew the grounding screw.
4. Remove the motor from the centrifuge by lifting vertically
5. To remove the motor suspension, remove the torsion
protection and unscrew the shock mounts for motor.
6. Unscrew the 3 M8 x 16 screws from the base plate and
place with the associated spring rings.

6.17.3 Install the centrifuge motor and motor suspension

When installing the motor suspension always replace the shock mounts for motor as a set!
Hint!

When installing the motor, do not premount the shock mounts for motor on the motor.
Hint!

1. Fit the 3 lock washers on the M8 x 16 screws and insert the screws into the base plate from below.
2. Mount the 3 shock mounts for motor.
3. Fit the torsion protection to the threaded bolts of the shock mounts for motor.
4. Fit the motor and fasten the grounding screw.
5. Fit the flat washers and curved spring washers. Fasten the motor with the 3 nuts.

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6 Disassembly/assembly

6.18 Rear panel with USB interface

6.18.1 Remove the rear panel

Prerequisites
Front panel with PCB Operating 5430 R has been removed (see p. 52).
1. Remove the screws of the rear panel.
2. Loosen the 6 housing screws.
3. Lift the housing slightly to the side to access the cable guide to the PCB Converter 5430 R.
4. Remove the rear panel with the USB interface.

6.19 Housing part

Injury from sharp-edged components.

Sharp-edged components are exposed when the device is open.

CAUTION!  Wear protective gloves.

 Work with extreme care.

6.19.1 Remove the housing part

Prerequisites
None
1. Remove two Phillips screws on each side.
2. Remove the housing part by lifting vertically.

6.20 Chamber sealing ring

Only slightly lift the housing to facilitate quicker removal/installation.

Hint!

6.20.1 Remove the chamber sealing ring

Prerequisites
The housing has been removed (see p. 52).
1. Remove the chamber sealing ring upwards.
2. Remove silicone residues.

6.20.2 Install the chamber sealing ring

1. Apply silicone rubber E 41.
2. Insert the chamber sealing ring.

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 Centrifuge 5430 R — Service manual - English

6 Disassembly/assembly

6.21 Condenser fan

6.21.1 Remove the condenser fan

Prerequisites
The housing has been removed (see p. 52).

1. Disconnect the plug connection to the PCB converter 5430 R.

2. Unscrew the two fastening screws (1)with a wrench (WAF=7 mm).

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6 Disassembly/assembly

6.22 Upper part of housing

You will need the assistance of a second person to remove and attach the upper part of housing.
Hint!

6.22.1 Dismantling the upper part of housing.

Prerequisites
• Front panel with PCB Operating 5430 R has been removed (see p. 52).
• The PCB Converter 5430 R has been removed (see p. 54).
• The console has been removed (see p. 53).
• The mains power switch has been removed (see p. 57).
• The rear panel has been removed (see p. 65).
• The housing attachment has been removed (see p. 65).

1. Remove the cover from the housing.

2. Unscrew the six housing screws with the service key (the screws remain attached to the housing and are attached by
silicone washers.
3. Remove the cold appliance plug and disconnect the plug connections.
4. Remove the grounding cable in the corner from the housing/rear panel.
5. Carefully remove the housing by lifting vertically.

6.23 Temperature sensor on the condenser

6.23.1 Replace the temperature sensor on the condenser

Prerequisites
The housing has been removed (see p. 67).
1. Disconnect the plug connection to the PCB converter 5430 R.
2. Separate the cable binders of the temperature sensor and open the insulation layer.
3. Remove the temperature sensor.

Use heat-conducting paste for installing the temperature sensor.

Hint!

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 Centrifuge 5430 R — Service manual - English

6 Disassembly/assembly

6.24 Opening the centrifuge lid if the emergency release is defective

Abb. 6-2: Position of the bores on the centrifuge lid

Front

370 mm
60 mm 60 mm
56 mm

10 mm
375 mm
64 mm

60 mm 60 mm
Fig. 6-2: Position of the bores on the centrifuge lid

Prerequisites
• Portable drill
• Drill for portable drill diameter 10 mm
• New upper part of lid for centrifuge, see "Spare parts" chapter.
1. Switch the device off.
2. Remove the mains cable.
3. Use the portable drill to bore 4 holes in the upper part of the lid.
The mandrels must break.
4. Remove the lid latch nuts.
5. Press the metal lid downward; twist off the lid latch nuts.
6. Remove the upper part of lid.

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7 Alignment/adjustment
7 Alignment/adjustment
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

7.1 Adjust the lid latch switch

Latch unit (left) Latch unit (right)

1 1

3
4

1 Spindle 2 Microswitch for lid position

3 Limit switch (left and right) 4 Hexagon drive of the emergency release

5 Latch motor

7.1.1 Lid latch functionality

The centrifuge lid is closed. This in turn closes the microswitch for the lid position and the magnet in the front side of the lid
(actuator) activates the reed switch. These two signals release the two latch motors. The spindles make contact with the
latch nuts of the lid. The limit switches are activated after the spindles have turned by 3 ½ revolutions. The two latch
motors are switched off. The centrifuge lid is latched.

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 Centrifuge 5430 R — Service manual - English

7 Alignment/adjustment

7.1.2 Requirements for setting switches

Requirements
• The centrifuge is completely assembled, only the front panel with PCB operating is removed.

Auxiliary equipment
• Ohmmeter
• Rotor key

The switch may only be set when it is deenergized; disconnect the centrifuge from the mains.
Hint!

Connections on the PCB converter, (see PCB converter 5430 R on p. 54).

7.1.3 Setting the microswitch for the lid position

Abb. 7-1: Latch unit (right)

2 3

Fig. 7-1: Latch unit (right)

1 Spindle 2 Microswitch for lid position

3 Limit switch 4 Allen clamp screw 2.5 mm

1. Loosen the microswitch cable from the X6 sealing ring.

Loosen the X6 sealing ring (position 3 and 4; cable colors: white/white).

Hint!

2. Clamp the ohmmeter to the cable; see Electronics overview in Chapter 8.

3. Fold down the centrifuge lid until the lid latch nuts rest completely on the spindles. The microswitch must have
triggered in this position. The reed switch should be activated.
4. To adjust the microswitch, loosen the clamping screw and change the position of the microswitch.

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7 Alignment/adjustment

7.1.4 Adjusting the right and left limit switches

Adjust the limit switches of the left and right latch unit individually. The procedure is identical.
First adjust the left limit switch. The lid is slightly higher because of the one-sided gas spring.
Hint!

Then adjust the right limit switch and check the left limit switch again.
The lid must be aligned to the front panel 5430 PCB operating.

1. Loosen the cables of the relevant limit switch from the X6 sealing ring and connect an ohmmeter, see electronics
overview in chapter 8.

Limit switch (right): loosen the X6 sealing ring (positions 1 and 2; cable colors: red/red).
Limit switch (left): loosen the X6 sealing ring (positions 5 and 6, cable colors: black/black).
Hint!

2. Close the centrifuge lid and lock with the emergency release. Turn the rotor key by one clockwise revolution.
3. Slowly turn the rotor key counterclockwise until the lid opens again. That is the position from which the spindle makes
contact in the latch nut of the lid during the latch operation.
4. Close the lid. Turn the rotor key by 3 ½ clockwise revolutions. The lid is now in latch position.
5. The limit switch must trigger in this position.
6. To adjust the limit switch, loosen the clamping screw if necessary and change the position of the microswitch.
7. To check the setting, repeat step 1 to 4. Correct the position of the microswitch if necessary.

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 Centrifuge 5430 R — Service manual - English

7 Alignment/adjustment

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 Centrifuge 5430 R — Service manual - English

8 Diagrams
8 Diagrams
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

8.1 Electronics layout plan

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 Centrifuge 5430 R — Service manual - English

8 Diagrams

8.2 PCB converter 5430R

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 Centrifuge 5430 R — Service manual - English

9 Maintenance
9 Maintenance
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

9.1 Cleaning and disinfection

9.1.1 Safety precautions

Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect the power plug before starting cleaning or disinfection
work.
DANGER!
 Do not allow any liquids to penetrate the inside of the housing.
 Do not spray clean/spray disinfect the housing.
 Only plug the device back in if it is completely dry, both inside and outside.

Infection by contaminated material.

There may be contaminated material on the device and accessories.

WARNING!  Work must only be completed on decontaminated devices.

 Find out more about contamination risks before beginning work.
 Check the device decontamination certificate.
 Wear personal protective equipment (protective gloves, protective goggles).

Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as strong and
weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.
NOTICE!
 If the device has been contaminated by aggressive chemicals, immediately clean it by means
of a mild cleaning agent.

Corrosion due to aggressive cleaning agents and disinfectants.

 Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.
NOTICE!  Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer
period of time.

Damage from UV and other high-energy radiation.

 Do not use UV, beta, gamma, or any other high-energy radiation for disinfecting.
NOTICE!  Avoid storage in areas with strong UV radiation

Autoclaving
All rotors, rotor lid, buckets and adapters apart from rotor A-2-MTP can be autoclaved (121°C,
Hint!

20 min).
Replace the lids of the aerosol-tight rotors after a maximum of 50 autoclaving cycles.

Aerosol-tightness
Before each use, check that the sealings are undamaged.
Hint!

Replace the rotor lid of the aerosol-tight rotors when the sealing rings on the lid screw and in the
lid groove become worn. The sealing rings require regular care to protect the rotors.
Aerosol-tight rotors should never be stored with lids screwed on!
In order to prevent damage, lightly lubricate the lid thread of the aerosol-tight rotors with pivot
grease (order no. Int.: 5810 350.050/North America: 022634330).

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 Centrifuge 5430 R — Service manual - English

9 Maintenance

Swing-bucket rotor
• Before cleaning the rotor, remove old pivot grease from grooves and pivots.
Hint!

• Check to see if the rotor grooves and pegs are clean, and clean them if necessary. If the
grooves and pegs are contaminated, the bucket may not swing out with a uniform motion.
• After cleaning, lubricate the pivots of the rotor and the grooves of the buckets with pivot
grease (order no. int.: 5810 350.050).

9.1.2 Cleaning agents and auxiliary equipment

Recommended cleaning agents:

• Alcohol 70% (ethanol, isopropanol)
• Mild neutral cleaning agent

Recommended lubricants:
• Pivot grease (order no. 5810 350.050)
• Glycerine, silicone grease
• High-performance grease "gleitmo WSP 5040" (order no. 5702 852.171)
• "gleitmo 300" spray
• Silicone rubber E 41
• Surface sealant (e.g., Loctite 5910)

Recommended auxiliary equipment:

• Protective gloves and goggles
• Cleaning brush/industrial vacuum cleaner
• Tools

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9 Maintenance

9.1.3 Cleaning and disinfecting the device

Additional notes and a detailed description on cleaning, disinfection and decontamination, can be
Hint! found in the operating manual of the device!
Please read the corresponding sections of the operating manual carefully before starting
cleaning, disinfection or decontamination!

If you have any questions on disinfection, decontamination, cleaning, or the cleaning agents to be
Hint! used, please contact Eppendorf AG Application Support. The contact details are provided on the
back of this manual.

1. Open the lid. Switch off the device with the mains power switch. Disconnect the power plug from the power supply.
2. Loosen the rotor nut by turning it counterclockwise with the rotor key.
3. Remove the rotor.
4. Clean and disinfect all accessible surfaces of the device, including the power cable, using a damp cloth and the
recommended cleaning fluids.
5. Thoroughly clean the rubber seals of the rotor chamber with water.
6. Let the rubber seal dry. To prevent the rubber seal from becoming brittle, rub the dry rubber seal with glycerol or
talcum. Other device components, e.g., the lid latch, lid springs, motor shaft and rotor cone, may not be lubricated.
7. Clean the motor shaft with a soft, dry and lint-free cloth. Do not lubricate the motor shaft.
8. Check the motor shaft for damage.
9. Inspect the device for corrosion and damage.
10. Leave the centrifuge lid open when the device is not in use.

9.1.4 Cleaning and disinfecting the rotor

Detailed descriptions of cleaning and maintenance procedures can be found in "Fixed-Angle

Hint! Rotor – Basic Inspection" and "Swing-Bucket Rotor – Basic Inspection".

1. Inspect the rotor and accessories for damage and rust. Do not use any damaged rotors or accessories.
2. Clean and disinfect the rotors and accessories with the recommended cleaning agents.
3. Use a bottle brush to clean and disinfect the rotor bores.
4. Rinse the rotors and accessories thoroughly with distilled water. Rinse the rotor bores of fixed-angle rotors particularly
thoroughly.

Do not immerse the rotor in liquid as liquid can get trapped inside the cavities.
Hint!

5. Place rotors and accessories on a cloth to dry. Place the fixed-angle rotors with the rotor bores facing downward to
allow the bores to dry.
6. Clean the rotor cone with a soft, dry and lint-free cloth. Do not lubricate the rotor cone.
7. Inspect the rotor cone for damage.
8. Place the dry rotor onto the motor shaft.
9. Tighten the rotor nut firmly by turning it clockwise with the rotor key.
10. Load the fixed-angle rotor with the cleaned adapters. Load the swing-bucket rotor with the cleaned buckets and
adapters.
11. Leave the rotor lid open when the rotor is not in use.

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 Centrifuge 5430 R — Service manual - English

9 Maintenance

9.2 Glass breakage

When using glass tubes there is a risk of glass breakage in the rotor chamber. The resulting glass splinters are swirled
around in the rotor chamber during centrifugation and have a sandblasting effect on the rotor and accessories. The
smallest glass particles become lodged in the rubber parts (e.g., the motor guide, the rotor chamber seal, and the rubber
mats of adapters).

Glass breakage in the rotor chamber

Glass tubes in the rotor chamber may break if the g-force is too high. Broken glass can damage
the rotor, accessories and samples.
NOTICE!
 Please note the manufacturer's information on the recommended centrifugation parameters
(load and speed).

Effects of glass breakage in the rotor chamber:

• Fine black metal abrasion in the rotor chamber (in metal rotor chambers)
• The surfaces of the rotor chamber and accessories are scratched.
• The chemical resistance of the rotor chamber is reduced.
• Contamination of samples
• Wear on rubber parts

How to proceed in case of glass breakage

1. Remove all splinters and glass powder from the rotor chamber and accessories.
2. Thoroughly clean the rotor and rotor chamber. Thoroughly clean the bores of the fixed-angle rotors, in particular.
3. If required, replace the rubber mats and adapters to prevent any further damage.
4. Regularly check the rotor bores for deposits and damage.

9.3 Shipping of the device

Risk to health from contaminated device

1. Please observe the information in the decontamination certificate, which is available as a
PDF file on our webpage (www.eppendorf.com/decontamination).
WARNING!
2. Decontaminate all the parts you would like to dispatch.
3. Include the fully completed decontamination certificate in the package.

Damage as a result of incorrect packing.

Eppendorf AG is not liable for damage caused by improper packing.
NOTICE!  The device may only be stored and transported in its original packaging.

Transport packaging can be ordered via your Eppendorf distribution partner. The order number
Hint! can be found in the spare parts list.

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 9.4
Group 1 Group 2 Group 3 Group 4
without refrigeration with refrigeration without refrigeration with refrigeration
no imbalance system no imbalance system with imbalance with imbalance
system system

KS/KT/2013-02-15
Maintenance services for centrifuges

5452
5418
5424
5702
5430
5804

5417C

5415R
5418R
5424R
5430R

5415D
9 Maintenance

5453
5810
5427R 5417R
5810R 5804R

Nr. Execution at least every months 12 12 12 12 12 12 12 12 12 12 12 5702RH 5702R

12 12 12 12
M 01 Cleaning of the device x x x x x x x x x x x x x x x
M 02 Cleaning the try for cendensation water and tube x x x x x x x
M 03 Checking the display x x x x x x x x x x x x x x x
M 04 Checking the keypad x x x x x x x x x x x x x x x
M 05 Checking for stability/housing x x x x x x x x x x x x x x x
Centrifuge maintenance overview

M 06 Checking the rotor chamber x x x x x x x x x x x x x x x

M 07 Cleaning and greasing the rotor chamber seal x x x x x x x x x x x
M 08 Checking the lid latch/emergency lid release x x x x x x x x x x x x x x
M 09 Checking the lid springs/lid shaft/gas spring x x x x x x x x x x x x x x
M 10 Cleaning and lubricating lid latch x x x x x x x x x x x x x x x
M 11 Checking the motor cap/speed sensor x x x x x x x
M 12 Checking the motor sleeve x x x x x x x
M 13 Checking the motor/motor shaft x x x x x x x x x x x x x x x
M 14 Checking the drive suspension/shock mounts x x x x x x x x x x x x x x x
M 15 Checking the imbalance detection x x x x x x x x
M 16 Checking the motor fan/device fan/vent flap x x x x x x x x x x x x x
Centrifuge 5430 R — Service manual - English

M 17 Checking the fuse base x x x x x x x x x x x

M 18 Checking the PCBs/brake resistor x x x x x x x x x x x x x x x
M 19 Checking the condenser fan x x x x x x x
M 20 Checking the compressor/condenser (heat exchanger) x x x x x x x
M 21 Checking the temperature sensors x x x x x x x
M 22 Evaluating, noting and possibly deleting device data x x x x x x x x x x x x x x x
M 23 Performing a software update x x x x x x x x x
M 24 Performing a test run x x x x x x x x x x x x x x x
Time consumption* up to 60 min 70 min 90 min 120 min

*The centrifuge housing is already disassembled.

79
 80
Group 1 Group 2 Group 3 Group 4
without refrigeration with refrigeration without refrigeration with refrigeration
no imbalance system no imbalance system with imbalance system with imbalance system

Operation Qualification (OQ)

5452
5418
5424
5702
5430
5804
incl. electrical safety test

5417C

5415R
5418R
5424R
5417R
5430R
5804R

5415D

5453
5810
5427R
5810R

5702 RH 5702R
Execution at least every months 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12
9 Maintenance

Operation Qualification (OQ) incl. electrical safety test x x x x x x x x x x x x x x x

Time consumption* up to 40 min 75 min 60 min 90 min

Group 1 Group 2 Group 3 Group 4

without refrigeration with refrigeration without refrigeration with refrigeration
no imbalance system no imbalance system with imbalance system with imbalance system

Additional maintenance
centrifuges

5452
5418
5424
5702
5430
5804

5417C

5415R
5418R
5424R
5417R
5430R
5804R

5415D

5453
5810
5427R
5810R

5702 RH 5702R

Nr. Execution at least every months 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24

ME 01 Replacing lid springs/gas spring x x x x x x x x x x x x x x
Time consumption* up to 15 min 15 min 15 min 15 min
ME 02 Replacing rotor chamber seal x x x x x x x x x x x
Time consumption* up to 15 min 20 min 20 min
ME 03 Replacing drive suspension/shock mounts x x x x x x x x x x x x x x x
Centrifuge 5430 R — Service manual - English

ME 04 Checking/completing imbalance compensation x x x x x x x x

Time consumption* up to 15 min 20 min 45 min 60 min
ME 05 Software upgrade x x x x x x x x x
Time consumption* up to 15 min 20 min
Execution at least every months 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12
ME 06 Electrical safety test x x x x x x x x x x x x x x x
Time consumption* up to 15 min 20 min 20 min 20 min
ME 07 Test duty in Germany (BGR 500) x x
Annual time consumption* about 90 min about 120 min
Time consumption* every three years about 150 min about 200 min

*The centrifuge housing is already disassembled.

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9 Maintenance

9.5 Maintenance services for centrifuges

M 01 Cleaning the device.

 Clean the centrifuge. Cleaning is described in the operating manual, in the Maintenance chapter of
the service manual and in the supplement sheets.

M 02 Cleaning the condensation water tray and tube

1. Drain the condensation water tray and remove it.
2. Check that the tube is unobstructed. Carefully blow it out with compressed air if necessary.

M 03 Checking the display

 Check the display using the corresponding service function.
Pay attention to the following:
• Defective pixels
• Backlight
• Illuminated keys (e.g., prog, stand by or open)
• Test the buzzer (if present)
 Replace the display if necessary.
M 04 Check the key pad
 Check the keypad using the corresponding service function, making sure to press all keys.
 Check the tactility. Pressing all keys should feel the same.
 Replace the keypad if necessary.
M 05 Checking stability
 Check the stability of the device feet.
 If cracks have developed on the device feet, replace them.
M 05 Checking the housing
 Clean the housing. Cleaning is described in the operating manual, in the Maintenance chapter of the
service manual and in the supplement sheets.
 Check the housing for cracks and replace if necessary. Liquid may enter the centrifuge through
cracks.
 Check the housing for any damage that may compromise safety and replace if necessary.
M 06 Checking the rotor chamber
Inspect the rotor chamber for damage. Replace the rotor chamber if necessary.
 Check rotor chambers which are made of plastic for cracks. Replace damaged rotor chambers.
 Clean the metal rotor chamber in accordance with the information in the operating manual. The rotor
is made from stainless steel or has a special coating.
 Check the rotor chamber for glass breakage, refer to the Glass breakage chapter.
 If samples with a high concentration of sodium chloride (NaCl) are used, spots which look like rust
adhesions may appear. Remove these adhesions with a polishing paste. Adhesions occur when the
maintenance measures recommended in the operating manual have not been followed.

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9 Maintenance

M 07 Cleaning and lubricating the rotor chamber sealing

 Clean the rotor chamber sealing as described in the corresponding operating manual and
supplement sheets.
 Perform maintenance measures. The care instructions are described in the operating manual and in
the supplement sheets (e. g. rub the seals with glycerine or grease).
Check the seals, paying attention to the following points:
• Crack formations
• Deformation
• Hardened seals
 Replace damaged seals.
Please note:
A defective seal allows more air to enter the rotor chamber. The penetrating humidity results in increased
ice formation in the rotor chamber. The cooling capacity of the centrifuge decreases.
A hard seal prevents the lid from closing correctly.

M 08 Checking the emergency release

 Check the emergency release, refer to the operating manual.
M 08 Check the lid latch reed switch.
 Check the reed switch using the corresponding service function.
 Also check the reed switch manually with a magnet.
M 08 Checking the latch mechanism of the lid latch
 Check the latch mechanism using the corresponding service function.
 Trigger the individual steps of the closing procedure by a separate keystroke.
 Remove the front panel or housing to get a better view of the phases of the lid latch.
Pay attention to the following parts:
• Lid latch
• Lid loops
• Slides and hooks
 Replace worn parts.
M 09 Check the lid springs and the lid shaft.
Check the lid springs and the lid shaft for visible wear, e.g. chafe marks.
5702 / 5702 R / 5702 R/ 5430:
 Lubricate the lid springs with "gleitmo WSP 5040" high-performance grease (order no. 5702
852.171).

M 09 Check the gas spring.

 Check the gas spring.
Keep the centrifuge lid open when the centrifuge is not in use.
Always open the centrifuge lid completely. Do not allow shelving above the centrifuge to restrict
movement.
 Observe the notes in the operating manual.

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9 Maintenance

M 10 Cleaning and greasing the lid latch

 Clean the lid latch with a degreasing agent (e.g., alcohol). Do not use acidic substances.

 Maintain the spindle of the lid latch units with "gleitmo 300" spray.
 Lubricate the lid latch nut with "gleitmo WSP 5040" high-performance grease (order no. 5702
852.171).

M 11 Checking the motor cap

 Replace the seals of the motor cap every two years. Only insert the motor cap of the Centrifuge 5430
R with silicone rubber E41.
 Check the seals.
Pay special attention to:
• Cracks
• Damage
• Centered, tension-free installation
Cracks and damage are caused by:
• Incorrectly sealed sealing clamp on the aerosol-tight caps.
• Tubes and jammed parts in the rotor chamber during centrifugation.
• Ingress of liquids.

M 11 Checking the speed sensor

 Insert the rotor and check the rotor detection and the rotor relation using the service function.
 Check the speed sensor using the customer service functions.
Note: Check the speed according to the OQ.

M 12 Checking the motor sleeve

Pay particular attention to the following items:
• Cracks
• Damage
• Correct installation (to ensure tightness)
Ingress of liquids may cause considerable damage.
• Paste the motor sleeve with Loctite 5910 to 6 locations in a circular motion.

M 13 Checking the motor

 Remove the rotor.
 Turn the motor shaft by hand. Operating noises and stiff movement indicate wear on the bearing.
 Remove the housing.
 Check the bearing shield of the motor for corrosion and cracks.
 Replace damaged parts.
M 13 Checking the motor shaft
 Remove the rotor.
 Check the motor shaft for cleanliness and wear. Replace the motor if necessary.
Scores and scratches on the motor shaft are caused by careless rotor assembly/disassembly. Scores
and scratches also occur if the rotor nut has not been tightened correctly.

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9 Maintenance

M 14 Checking the drive suspension

If the shock mounts are mounted horizontally, the drive suspension will be especially stressed.
 Check the drive suspension for cracks.
M 14 Checking the shock mounts for motor
During lateral assembly the shock mounts for motor are exposed to shearing forces and the weight of
the loaded rotor. The shock mounts may develop cracks or may become deformed.
Check the shock mounts for motor, paying attention to the following points:
• Cracks
• Damage
• Deformation
 Replace the shock mounts for motor for motor every two years.
 Only replace shock mounts for motor together as a set (3 pcs.).
M 15 Checking imbalance detection
1. Adjust the imbalance switch as described in the Alignment/Adjustment chapter of the service
manual.
2. Using the corresponding customer service function, check whether the tolerance is observed for the
adjustment.

M 16 Checking the motor fan/device fan

 Use a cleaning brush or compressed air to remove any dust and contamination. Remove
contamination with an industrial vacuum.
 Listen for running noises.
 Select the different program steps for testing using the corresponding service function
 If the centrifuge is used frequently, replace the fan as a precautionary measure.
M 16 Checking the ventilation flap
 Check whether the ventilation flap opens. For this, use the corresponding service function. The
ventilation flap only opens during operation when the fixed-angle rotor is inserted.

M 17 Checking the fuse holder

 Remove the fuses. Check the fuse holder contacts and the fuse mount for oxidation and deposits.
M 18 Checking the PCBs and brake resistor
 Use a cleaning brush or compressed air to remove any dust and contamination on the PCB.
 Check any available brake resistors using an ohmmeter.
M 19 Checking the condens fan
 Use a cleaning brush or compressed air to remove any dust and contamination.
 Listen for running noises.
 Check the different control stages using the corresponding service function.
M 20 Checking the compressor
 Check switching the compressor on and off using the corresponding service function.
M 20 Check the condenser (heat exchanger).
 Use a cleaning brush or compressed air to remove any dust and contamination.

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9 Maintenance

M 21 Check the temperature sensor at the bottom of the chamber.

 Display the current temperature in the display using the corresponding service function.
After the centrifuge has been at a standstill for an extended period with the lid open, the displayed
temperature must match the ambient temperature.
Note: Check the temperature according to the OQ.

M 22 Evaluate the device date, write it down and delete, if required.

 Call the following parameters with the corresponding service function or customer service function:
• Error list
• Error history (last 4 errors)
• Number of activations
• Operating hours
• Total number of all rotor runs
• Number of runs per rotor type
Note: The parameters that are displayed may vary according to the type of centrifuge.

M 23 Carrying out a software update.

Software updates are provided free of charge as part of maintenance so that advantage can be taken of
program improvements.
A software update is performed by replacing an EPROM or via the USB interface.
You can download the latest software versions from the www.eppendorf-support.com website.

M 24 Carrying out a test run.

1. Insert the rotor with accessories.
2. Load the rotor with the maximum number of intended tubes. Fill the tube with ethylene glycol.
3. Only refrigerated centrifuges: Start run by pressing the fast temp key. 6°C should be reached
quickly (rotor-dependent, note the lowest achievable temperature).
4. Perform the test run for 10 minutes at the maximum speed.
Additional information can be found in the operating manual.

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9 Maintenance

9.6 Extra maintenance services for centrifuges

ME 01 Replacing lid springs

See "Disassembly/assembly" chapter.

ME 01 Replacing the gas spring

See "Disassembly/assembly" chapter.

ME 02 Replacing the rotor chamber seal

See "Disassembly/assembly" chapter.

ME 03 Replacing the drive suspension/shock mount

See "Disassembly/assembly" chapter.

ME 04 Performing an imbalance compensation

See the "Alignment/adjustment" chapter or the service function in the "Troubleshooting" chapter.

ME 05 Software upgrade
 Install the latest software upgrades (for a free) to take advantage of product improvements via new
functions, e.g., retrofitting a rotor.
A new version of the basic software will be installed.
A software upgrade is performed by replacing an EPROM or via the USB interface.
You can download the latest software versions from our website at www.eppendorf-support.com.

ME 06 Electrical safety test

See the "Description of device test for centrifuges" chapter.

ME 07 Test duty in Germany (BGR 500)

In Germany, accident prevention regulations BGR 500 (previously VBG 7z) apply. Centrifuges with a
nominal output >500 W or a kinetic energy > 10,000 Nm are subject to testing.
The following Eppendorf AG centrifuges are subject to testing: Centrifuge 5804, 5804 R, 5810, 5810 R,
5810 RA.
These centrifuges must be inspected annually when in operation and at least every three years in a
disassembled state.
Rotors, with a kinetic energy  10.000 Nm are not subject to testing. Rotors which are used in
centrifuges which are subject to testing must be tested, regardless of their output.
The operator of the centrifuge is responsible for documenting and retaining the results of such tests.

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9 Maintenance

9.7 Device tests for centrifuges

9.7.1 Speed check

Measuring device
• Digital hand-held tachometer, suitable for non-contact measuring or a stroboscope
• Measurement range: 1 to  30,000 rpm
• Accuracy: 0.1%

Carry out measurement:

1. Install the rotor.
2. Fully load the rotor with filled tubes.
3. Mark the rotor hub with a reflector (measuring device accessory).
4. Close the centrifuge lid.
5. Start the centrifuge with the required speed and let it run briefly until the speed has stabilized.
6. Measure the speed through the monitoring glass in the centrifuge lid. Observe the operating instructions for the
measuring device for this.
7. Note the measuring result.
8. If required, repeat steps 5 to 7 for other rotational speeds.
The speed must be checked with the centrifuge lid closed. An inspection with the centrifuge lid open is not permitted due
to safety reasons!
A recommendation on the rotational speeds to be tested, and their tolerances, can be found in the technical data or the
OQ of the corresponding centrifuge.

9.7.2 Temperature check

Ambient temperature: 23°C ± 2°C

Measuring device
• Thermometer with a temperature sensor, with a low specific heat capacity (e.g. thermocouple, max. Ø 0.5 mm)
• Measurement range: 0 °C to  100 °C
• Accuracy:  0.1°C

Auxiliary equipment
• Rotor
• Vessels that fit the rotor, with min. volume of 1.5 mL

Refrigerated centrifuges
1. Install the rotor and load it symmetrically with the tubes.
2. Fill the vessels with ethylene glycol.
3. Set the maximum rotational speed.
4. Set the minimum achievable temperature.
5. Precool the rotor with the Fast temp key.
6. Start the centrifuge and stop it after 45 min.
7. Measure the temperature in the center of the tubes immediately after opening.
8. Compare the sample temperature with the temperature displayed by the centrifuge.

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9 Maintenance

Non-refrigerated centrifuges: sample heating compared to room temperature

1. Install the rotor and load it symmetrically with the tubes.
2. Fill the vessels with ethylene glycol.
3. Set the maximum rotational speed.
4. Start the centrifuge and stop it after 45 min.
5. Measure the ambient temperature.
6. Stop the centrifuge and measure the temperature in the center of the tubes immediately after opening.
7. Compare the ambient temperature and sample temperature.
A recommendation on the temperatures to be tested, and their tolerances, can be found in the technical data or the OQ of
the corresponding centrifuge.

9.7.3 Timer check

Measuring device
• Stopwatch
• Accuracy:  0.1 seconds

Carry out measurement:

1. Set the run time to continuous operation.
2. Start the centrifuge and stopwatch at the same time.
3. Allow the centrifuge to run at least 5 min.
4. Compare the time displayed by the centrifuge with the time measured by the stopwatch.
A recommendation on the times to be tested and, their tolerances can be found in the technical data or the OQ of the
corresponding centrifuge.

9.7.4 Electrical safety test

Effective standard
• IEC 62638 (international)
• VDE 701/702 (Germany)
• NFPA 99 (USA)

Notes concerning the measuring procedure:

• The electrical safety test may only be carried out by a suitably trained electrician.
• Measure on a grounded, bare metallic point that can be touched from the outside (e.g. housing screws).
• The protective earth conductor leakage current or the protective earth conductor resistance cannot be measuredOn
the centrifuge lid, rotor or the motor shaft.
• Never measure on any interfaces that may be present. This might cause damage to the centrifuge electronics.
• Due to the internal electrical design of the device, the equivalent leakage current cannot be measured.

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10 Technical data
10 Technical data
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

10.1 Power supply

Centrifuge 5430 R

Mains connection 230 V, 50 to 60 Hz

120 V, 50 to 60 Hz
100 V, 50 to 60 Hz
Current consumption 6 A (230 V)
12 A (120 V/ 100 V)
Power consumption Maximum 1050 W
EMC: interference emission (radio interference) EN 61326 – Class B (230 V/120 V)
EN 61326 – Class A (100 V)
EMC: noise immunity EN 61326
Overvoltage category II
Protection class 1
Fuses Thermal overcurrent protective switch 7 A (230 V)
Thermal overcurrent protective switch 15 A (120 V/100 V)

10.2 Ambient conditions

Ambience For indoor use only.

Ambient temperature Centrifuge 5430 R: 10 to 40°C
Maximum relative humidity 75 %, non-condensing humidity
Atmospheric pressure Use up to an altitude of 2000 m above MSL.
Degree of pollution 2

10.3 Application parameters

Run time 30 s to 99:59 h, infinity ( )

Adjustable to 10 min in 0.5 min increments, then
increments of 1 min
Temperature of 5430 R -11°C to 40°C
Relative centrifugal force (or rcf) 1 to 30,130 x g
Adjustable to 3.000 x g in increments of 10 x g, thereafter
in increments of 100 x g
Speed 100 to 17,500 rpm
Adjustable to 5,000 rpm in increments of 10 rpm, thereafter
in increments of 100 rpm
Maximum load 48 tubes with 2.0 mL each or 6 conical tubes with 50 mL
each
Maximum kinetic energy 10,000 nm
Compulsory test log book No
Permitted density of the material for centrifuging 1.2 g/mL
at max. g-force/rpm and max. load

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10 Technical data

Rotor Lowest achievable temperature Speed to safely maintain sample

temperature of 4°C
-11°C set
4°C set
23°C ambient temperature
23°C ambient temperature
60 min run time

FA-45-48-11 < 0°C 12 700 rpm

F-45-48-11 < 0°C 12 700 rpm

FA-45-30-11 < 0°C 14 000 rpm

F-45-30-11 < 0°C 14 000 rpm

FA-45-24-11-HS < 5°C ? rpm

FA-45-24-11-Kit < 0°C 13 200 rpm

F-45-64-5-PCR < 0°C 11 800 rpm

F-45-18-17-Cryo < 0°C 8 900 rpm

FA-45-16-17 < 0°C 14 200 rpm

F-35-6-30 < 0°C 7 830 rpm

A-2-MTP < 0°C 4 680 rpm

S-24-11-AT < 0°C 12 700 rpm

Rotor Acceleration time/Deceleration time Without soft ramp

Acceleration time/Deceleration time With soft ramp
230 V 120 V 100 V
FA-45-48-11  20 / 20 s  20 / 20 s  27 / 20 s
F-45-48-11  61 / 65 s (SOFT)  61 / 65 s (SOFT)  61 / 65 s (SOFT)
FA-45-30-11  15 / 15 s  15 / 15 s  20 / 15 s
F-45-30-11  61 / 65 s (SOFT)  61 / 65 s (SOFT)  61 / 65 s (SOFT)
FA-45-24-11-Kit  15 / 16 s  15 / 16 s  20 / 16 s
 78 / 90 s (SOFT)  78 / 90 s (SOFT)  78 / 90 s (SOFT)
FA-45-16-17  20 / 20 s  20 / 20 s  30 / 20 s
 61 / 66 s (SOFT)  61 / 66 s (SOFT)  61 / 66 s (SOFT)
S-24-11-AT  13 / 16 s  13 / 16 s  16 / 16 s
 61 / 66 s (SOFT)  61 / 66 s (SOFT)  61 / 66 s (SOFT)
FA-45-24-11-HS  21 / 16 s  21 / 16 s  30 / 16 s
 60 / 65 s (SOFT)  60 / 65 s (SOFT)  60 / 65 s (SOFT)
F-45-64-5-PCR  12 / 15 s  12 / 15 s  15 / 15 s
 62 / 65 s (SOFT)  62 / 65 s (SOFT)  62 / 65 s (SOFT)
F-45-18-17-Cryo  8 / 11 s  8 / 11 s  8 / 11 s
 77 / 85 s (SOFT)  77 / 85 s (SOFT)  77 / 85 s (SOFT)
F-35-6-30  23 / 23 s  23 / 23 s  27 / 27 s
 62 / 67 s (SOFT)  62 / 67 s (SOFT)  62 / 67 s (SOFT)
A-2-MTP  18 / 21 s  18 / 21 s  18 / 21 s
 63 / 67 s (SOFT)  63 / 67 s (SOFT)  63 / 67 s (SOFT)

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10 Technical data

10.4 Weight/dimensions
Centrifuge 5430 R

Dimensions Width: 380 mm (15.0 in.)

Depth: 640 mm (25.2 in.)
Height: 296 mm (11.7 in.)
Weight without rotor 56 kg (123.5 lbs.)
Noise level < 60 dB(A) *

*) The noise level was measured frontally in a sound measuring room with accuracy class 1, 1 m from the device and at
lab bench height.

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10 Technical data

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11 Ordering information
11 Ordering information
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

11.1 Images of spare parts

Abb. 11-1: General view

1-3

1-3
1-1

1-2

1-10

2-2

2-9

5-1

3-1

Fig. 11-1: General view

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11 Ordering information
Abb. 11-2: Front panel

3-2

Fig. 11-2: Front panel

Abb. 11-3: Rear side of the front panel with PCB controller

Fig. 11-3: Rear side of the front panel with PCB controller

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11 Ordering information
Abb. 11-4: Side view with power supply

7-1 2-3 7-2

Fig. 11-4: Side view with power supply

Abb. 11-5: View from below with device feet

2-1 2-1

Fig. 11-5: View from below with device feet

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11 Ordering information
Abb. 11-6: Rear view

2-7

2-8

2-11

Fig. 11-6: Rear view

Abb. 11-7: Lid latch

1-5

6-1

1-6

Fig. 11-7: Lid latch

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11 Ordering information
Abb. 11-8: PCB converter

6-8

Fig. 11-8: PCB converter

Abb. 11-9: Console

2-10

2-6

Fig. 11-9: Console

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11 Ordering information
Abb. 11-10: Fan for motor and converter

5-3
5-4

Fig. 11-10: Fan for motor and converter

Abb. 11-11: Brake resistor

3-6

Fig. 11-11: Brake resistor

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11 Ordering information
Abb. 11-12: Gas spring

1-8

1-8

1-9

1-7

Fig. 11-12: Gas spring

Abb. 11-13: Latch unit

6-6 6-7 6-5

6-4 6-4

6-3 6-2

Fig. 11-13: Latch unit

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11 Ordering information
Abb. 11-14: Drive motor with PCB speed/imbalance

3-7

4-1

Fig. 11-14: Drive motor with PCB speed/imbalance

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11 Ordering information
Abb. 11-15: Motor cap with integrated rotor sensor

4-2

Fig. 11-15: Motor cap with integrated rotor sensor

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11 Ordering information
Abb. 11-16: Motor covers

4-6

4-3

4-4
4-5

Fig. 11-16: Motor covers

Abb. 11-17: Cooling unit

8-1

8-4
8-5
8-

5-2
8-3
8-2

5-5

Fig. 11-17: Cooling unit

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11 Ordering information
Abb. 11-18: PCB standby

Fig. 11-18: PCB standby

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11 Ordering information

11.2 Spare parts

11.2.1 Lid

Fig. Item Order no. Description

(international)
11-1 1-1 5428 850.019 Upper part of lid 5430 R
Lid panel 5430 R
11-1 1-2 5428 850.264 with monitoring glass
Lid 5430 R
11-1 1-3 5428 850.213 with upper part of lid, lid panel, monitor glass and lid latch nuts
11-7 1-5 5428 850.035 Magnet for lid position (actuator)
11-7 1-6 5428 850.051 Lid axle
incl. 2 circlips
11-12 1-7 5428 850.078 Spring lifter 5430 R
incl. circlips and retaining pins
11-12 1-8 5428 850.086 2 retaining pins for spring lifter and 10 circlips
11-12 1-9 5428 850.167 Damping ring for spring lifter 5430 R
11-1 1-10 5428 850.094 Rubber sleeve spring lifter/housing

11.2.2 Housing

Fig. Item Order no. Description

(international)
Foot for device
11-5 2-1 5428 850.108 set of 4
Cover cap
11-1 2-2 5426 853.290 10 pieces
11-4 2-3 5428 850.124 Upper part of housing 5430 R
incl. housing label, screws/washers and front sealing strip
----- 2-4 5428 850.132 Washers for housing 5430 R
10 pieces
----- 2-5 5428 850.140 Sealing strip
at the front of the housing 5430 R, 10 pieces
11-9 2-6 5428 850.159 Guide emergency lid release
11-6 2-7 5428 850.175 Housing attachment 5430 R
11-6 2-8 5428 850.183 Compressor holder angle, top
11-1 2-9 5428 850.116 Sealing ring for rotor bowl
Console 5430 R
11-9 2-10 5428 850.191 120 / 230 V
11-9 2-10 5428 850.248 100 V
11-6 2-11 5428 850.205 Rear panel 5430 R
incl. USB interface and cable

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11 Ordering information

11.2.3 Electronics

Fig. Item Order no. Description

(international)
11-1 3-1 5428 851.228 Front panel for keypad 5430R
with PCB operation (display software from version 3.0)
11-2 3-2 5428 851.236 Front panel for rotary knob 5430R
with PCB operation (display software from version 3.0)
11-3 3-3 5428 850.272 Front panel ribbon cable
PCB converter 5430 R
up to serial no. 12.999; mainboard software up to version 1.x
11-8 3-4 5428 850.302 230 V
11-8 3-4 5428 850.310 120 V
PCB converter 5430 R
as of serial no. 13.000; mainboard software as of version 2.1
11-8 3-4 5428 851.309 230 V
11-8 3-4 5428 851.317 120 V
11-8 3-4 5428 851.333 100 V
PCB converter/power line filter 5430 R
up to serial no. 12.999; mainboard software up to version 1.x
----- 3-5 5428 851.325 100 V
Brake resistor
11-11 3-6 5428 850.337 100 V to 230 V (160 Ohm)
PCB Rotational speed/imbalance
11-14 3-7 5428 850.256 5430R
11-18 3-8 5428 851.376 PCB Standby 5430 R

11.2.4 Drive

Fig. Item Order no. Description

(international)
Centrifuge motor
incl. earthing strap
11-14 4-1 5428 850.353 100 V to 230 V
11-15 4-2 5428 850.299 Motor suspension 5430R
set of 3, each consisting of 3 pieces: screw M8 x 16, lock ring A8, shock mount
for motor, torsion protection, flat washer Da=16, lock washer A8, nut M8
11-16 4-3 5428 850.361 Motor cap with integrated rotor sensor 5430 R
11-16 4-4 5428 850.370 Motor sleeve 5430 R
11-16 4-5 5428 850.388 Insulating cap 5430 R
11-16 4-6 5428 850.400 Motor sleeve back-up ring 5430R

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11 Ordering information

11.2.5 Temperature sensors / fans

Fig. Item Order no. Description

(international)
Temperature sensor
11-1 5-1 5428 850.426 bottom of rotor bowl
11-17 5-2 5428 850.434 condenser
11-10 5-3 5428 850.442 Fan for motor 5430 R
11-10 5-4 5428 850.450 Fan for PCB converter 5430 R
230 V all devices/120 V up to serial number 12.999
Fan for PCB converter 5430 R
100 V all devices/120 V as of serial no. 13.000
11-10 5-4 5428 855.452 100 V/120 V
Condenser fan
11-17 5-5 5428 850.469 100 V/120 V
11-17 5-5 5428 850.477 230 V

11.2.6 Lid latch

Fig. Item Order no. Description

(international)
11-7 6-1 5427 850.082 Retaining cap for lid latch
Set of 2 pieces
Lid latch unit
with lid latch nut and lid latch retaining cap
11-13 6-2 5428 851.015 right
11-13 6-3 5428 851.023 left
11-13 6-4 5428 850.060 Lid latch nut
with damping plate, retaining plate for lid latch and screws
Micro switch end position
11-13 6-5 5428 851.040 right, red wires, incl. 2 screws
11-13 6-6 5427 851.046 left, black wires, incl. 2 screws
Micro switch for lid position
11-13 6-7 5427 851.054 white wires, incl. 2 screws
11-8 6-8 5428 851.031 Reed switch for the lid position
Rubber-metal dampers for locking motor
Set of 2
----- 6-9 5427 851.089 with screws, nuts, washers, and torsion protections.
Lid latch motor
with cable and clutch
----- 6-10 5427 851.143 left for left-hand thread
----- 6-11 5428 851.139 right for left-hand thread

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11 Ordering information

11.2.7 Mains connection

Detailed ordering information on power cables can be found in the operating manual.

Fig. Item Order no. Description

(international)
Mains switch 5430 R
with thermal fuse
11-4 7-1 5428 850.485 230 V
11-4 7-1 5428 850.493 100 V/120 V
Mains input module
11-4 7-2 0113 200.693 10 A (100 - 230 V)

11.2.8 Refrigeration system

Fig. Item Order no. Description

(international)
Condenser 5430 R
11-17 8-1 5428 850.515 top
11-17 8-2 5428 850.523 bottom
Compressor 5430 R
100 - 240 V / 50-60 Hz
11-17 8-3 5428 850.531 up to serial number 12.999
----- 8-3 5428 851.538 as of serial no. 13.000
11-17 8-4 5428 850.540 Filter dryer 5430 R
Injection valve 5430 R
11-17 8-5 5428 850.558 up to serial number 12.999
----- 8-5 5428 851.554 as of serial no. 13.000

11.2.9 Accessories
Detailed ordering information can be found in the operating manual.

Fig. Item Order no. Description

(international)
Rotor key
----- 9-1 5416 301.001 Standard
Pivot grease
----- 9-2 5810 350.050 Tube 20 mL
High performance grease gleitmo WSP 5040
----- 9-3 5702 852.171 5 grams bag
----- 9-4 5427 728.007 Rotor removal tool for Rotor F-35-6-30
----- 9-5 5428 850.620 Service key for housing
USB software cable
----- 9-6 5428 850.280 USB-A/USB-B
----- 9-7 5428 850.604 Universal transport packaging for 5430 R
(excl. or incl. rotor) incl. transport safety
----- 9-8 5428 850.418 Tray for condensation water
Service manual 5430 R
----- 9-9 5428 920.009 german
----- 9-9 5428 920.017 english

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11 Ordering information

11.3 Rotors, rotor lids and gaskets

11.3.1 Rotors with QuickLock rotor lid

Rotor FA-45-48-11
Order no. Description
(international)
Fixed-angle rotor FA-45-48-11
5427 754.008 aerosol-tight, angle 45°, 48 places, max. tube diameter 11 mm, incl. rotor lid (aluminum)
Rotor lid for FA-45-48-11
5427 762.000 aerosol-tight, aluminum
Seal for rotor lid
FA-45-48-11 (5427 R/5430/5430 R)
5409 718.002 set of 5 pieces

Rotor FA-45-30-11
Order no. Description
(international)
Fixed-angle FA-45-30-11
5427 753.001 aerosol-tight, angle 45°, 30 places, max. tube diameter 11 mm, incl. rotor lid (aluminum)
Rotor lid for FA-45-30-11
5427 761.004 aerosol-tight, aluminum
Seal for rotor lid
FA-45-30-11 (5427 R/5430/5430 R)
5820 762.004 Set of 5 pieces

Rotor FA-45-24-11 Kit

Order no. Description
(international)
Fixed-angle rotor FA-45-24-11-Kit
5427 752.005 aerosol-tight, angle 45°, 24 places, max. tube diameter 11 mm, incl. rotor lid (aluminum)
Rotor lid for FA-45-24-11-Kit
5427 760.008 aerosol-tight, aluminum
Seal for rotor lid
FA-45-24-11-Kit (5427 R/5430/5430 R), FA-45-48-11, FA-45-20-17 (5804/5804 R/5810/5810 R)
5820 767.006 Set of 5 pieces

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11 Ordering information

Rotor FA-45-16-17
Order no. Description
(international)
Fixed-angle rotor FA-45-16-17
5427 750.002 aerosol-tight, angle 45°, 16 places, max. tube diameter 17 mm, incl. rotor lid (aluminum)
Rotor lid for FA-45-16-17
5427 751.009 aerosol-tight, aluminum
Seal for rotor lid
FA-45-24-11 (5427 R), FA-45-16-17 (5430/5430 R)
5409 717.006 Set of 5 pieces

Rotor S-24-11-AT
Order no. Description
(international)
Swing-bucket rotor S-24-11-AT
5427 757.007 aerosol-tight, steel, angle 90°, 24 places, max. tube diameter 11 mm, incl. rotor lid (aluminum)
Rotor lid for S-24-11-AT
5427 758.003 aerosol-tight, aluminum
Seal for rotor lid
S-24-11-AT (5427 R/5430/5430 R)
5409 719.009 set of 5 pieces
Tube holder for S-24-11-AT
for 4 × 1,5 mL/2,0 mL Eppendorf tubes
5409 721.003 set of 2 pieces

11.3.2 Rotors with rotor lid thread

Rotor F-45-48-11
Order no. Description
(international)
Rotor F-45-48-11
5409 712.004 aluminum, angle 45°, 48 places, max. tube diameter 11 mm, incl. rotor lid (polypropylene)
Rotor lid for F-45-48-11
5409 713.000 Polypropylene

Rotor FA-45-30-11
Order no. Description
(international)
Rotor lid
for F-45-30-11
5427 719.008 aerosol-tight, PTFE-coated, aluminum

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11 Ordering information

Rotor F-45-30-11
Order no. Description
(international)
Rotor F-45-30-11
5427 712.003 PTFE-coated, angle 45°, 30 places, max. tube diameter 11 mm, incl. rotor lid (polypropylene)
Rotor lid
for F-45-30-11
5427 718.001 Polypropylene

Rotor FA-45-24-11-HS
Order no. Description
(international)
Rotor FA-45-24-11-HS
aerosol-tight, PTFE-coated, angle 45°, 24 places, max. tube diameter 11 mm, incl. rotor lid
5427 710.000 (aluminum), incl. rotor key
Rotor lid
for FA-45-24-11-HS
5427 711.007 aerosol-tight, PTFE-coated, aluminum

Rotor FA-45-24-11-Kit
Order no. Description
(international)
Rotor lid
for FA-45-24-11-Kit
5427 704.000 aerosol-tight, aluminum

Rotor F-45-64-5-PCR
Order no. Description
(international)
Rotor F-45-64-5-PCR
5427 714.006 angle 45°, 64 places, max. tube diameter 5 mm, incl. rotor lid (aluminum) and adapters
Rotor lid
for F-45-64-5-PCR
5427 720.006 aluminum

Rotor F-45-18-17-Cryo
Order no. Description
(international)
Rotor F-45-18-17-Cryo
5427 705.007 angle 45°, 18 places, max. tube diameter 17 mm, incl. rotor lid (polyproplene) and adapters
Rotor lid
for F-45-18-17-Cryo
5427 707.000 Polypropylene

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11 Ordering information

Rotor F-35-6-30
Order no. Description
(international)
Rotor F-35-6-30
angle 35°, 6 places, max. tube diameter 30 mm, incl. rotor lid (aluminum) and adapters for 15/
5427 716.009 50 mL conical tubes
5427 739.009 angle 35°, 6 places, max. tube diameter 30 mm, incl. rotor lid
Rotor lid
for F-35-6-30
5427 715.002 aluminum

11.3.3 Rotors with rotor lid for attaching

A-2-MTP rotor
Order no. Description
(international)
Rotor A-2-MTP
5427 700.005 with 2 buckets and windshield upper shell
Spare MTP buckets for A-2-MTP
5427 722.009 Set of 2
Wind shield upper shell for A-2-MTP
5427 725.008 aluminum

The Aerosol tightness is tested and certified by the Centre of Emergency Preparedness and
Hint! Response, Health Protection Agency, Porton Down (UK).

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11 Ordering information

11.4 Suggested spare parts

11.4.1 Lid

Fig. Item Order no. Description

(international)
11-12 1-7 5428 850.078 Spring lifter 5430 R
incl. circlips and retaining pins

11.4.2 Housing

Fig. Item Order no. Description

(international)
11-1 2-9 5428 850.116 Sealing ring for rotor bowl

11.4.3 Electronics

Fig. Item Order no. Description

(international)
11-1 3-1 5428 851.228 Front panel for keypad 5430R
with PCB operation (display software from version 3.0)
11-2 3-2 5428 851.236 Front panel for rotary knob 5430R
with PCB operation (display software from version 3.0)
11-3 3-3 5428 850.272 Front panel ribbon cable
PCB converter 5430 R
up to serial no. 12.999; mainboard software up to version 1.x
11-8 3-4 5428 850.302 230 V
11-8 3-4 5428 850.310 120 V
PCB converter 5430 R
as of serial no. 13.000; mainboard software as of version 2.1
11-8 3-4 5428 851.309 230 V
11-8 3-4 5428 851.317 120 V
11-8 3-4 5428 851.333 100 V
PCB converter/power line filter 5430 R
up to serial no. 12.999; mainboard software up to version 1.x
---- 3-5 5428 851.325 100 V
Brake resistor
11-11 3-6 5428 850.337 100 V to 230 V (160 Ohm)
PCB Rotational speed/imbalance
11-14 3-7 5428 850.256 5430R

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11 Ordering information

11.4.4 Drive
Fig. Item Order no. Description
(international)
Centrifuge motor
incl. earthing strap
11-14 4-1 5428 850.353 100 V to 230 V
11-15 4-2 5428 850.299 Motor suspension 5430R
set of 3, each consisting of 3 pieces: screw M8 x 16, lock ring A8, shock mount
for motor, torsion protection, flat washer Da=16, lock washer A8, nut M8
11-16 4-3 5428 850.361 Motor cap with integrated rotor sensor 5430 R
11-16 4-4 5428 850.370 Motor sleeve 5430 R
11-16 4-5 5428 850.388 Insulating cap 5430 R
11-16 4-6 5428 850.400 Motor sleeve back-up ring 5430R

11.4.5 Temperature sensors / fans

Fig. Item Order no. Description
(international)
Temperature sensor
11-1 5-1 5428 850.426 bottom of rotor bowl
11-17 5-2 5428 850.434 condenser

11.4.6 Lid latch

Fig. Item Order no. Description
(international)
11-7 6-1 5427 850.082 Retaining cap for lid latch
Set of 2 pieces
11-13 6-4 5428 850.060 Lid latch nut
with damping plate, retaining plate for lid latch and screws
Micro switch end position
11-13 6-5 5428 851.040 right, red wires, incl. 2 screws
11-13 6-6 5427 851.046 left, black wires, incl. 2 screws
Micro switch for lid position
11-13 6-7 5427 851.054 white wires, incl. 2 screws
11-8 6-8 5428 851.031 Reed switch for the lid position
Lid latch motor
with cable and clutch
----- 6-10 5427 851.143 left for left-hand thread
----- 6-11 5428 851.139 right for left-hand thread

11.4.7 Mains supply

Fig. Item Order no. Description
(international)
Mains switch 5430 R
with thermal fuse
11-4 7-1 5428 850.485 230 V
11-4 7-1 5428 850.493 100 V/120 V
Mains input module
11-4 7-2 0113 200.693 10 A (100 - 230 V)

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11 Ordering information

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12 Technical information
12 Technical information
Service
Centrifuge
on
see
p.
Tab.
Fig.
—p. manual5430 -REnglish

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