Savitribai Phule Pune University, Pune invites tenders for the purchase of “ Tissue Culture Lab ”
for, Department of Chemistry. Savitribai Phule Pune University, Pune hereinafter is referred as
“BUYER” / “purchaser” and Tenderer is referred as “BIDDER”/ “supplier.”
1. Technical specifications of the instrument are as per the attached
ANNEXURE (A) Technical Specifications : Tissue Culture Lab
2. The BIDDER should submit offers into two parts – Part A should include the technical offer and
Part B should be financial offer.
3. The supplier shall pay and bear all other liabilities, taxes and duties not specifically agreed by
the Purchaser in the commercial offer.
4. The leaflets catalogue, etc. should be attached invariably so that a proper evaluation of the
equipment offered is possible.
5. Pre installation requirements for the instrument (power specifications, space required etc)
should be mentioned clearly.
6. All communications are to be addressed to “The Head, Department of Chemistry”, Savitribai
Phule Pune University, Pune - 411 007.
7. The quantity of items is in the discretion of BUYER.
8. Accessories and item procured in India should be quoted in Indian rupees.
9. GST / Other Govt. Taxes: Excise Duty (ED), other taxes, levies, like Service Tax (ST), are to be
indicated separately. BIDDER should mention GST Registration Number, PAN Number and
required to be necessarily indicated in the offer.
10. DELIVERY: The successful BIDDER should deliver the material as per tender
document/purchase order within 75 days from the date of acceptance of the purchase order.
11. BUYER will evaluate technical and commercial acceptable offers in its approved Technical
Committee and/or Purchase Committee. Technical Committee/ Purchase Committee will have
right to accept or reject the BIDDER. The Technical Committee Purchase Committee will
decide to accept the required configuration, items and other requirements in its discretion.
12. CANCELLATION OF TENDER: Not withstanding anything specified in this tender document,
BUYER in its sole discretion, unconditionally and without having to assigned any reasons,
reserves the rights:
a) To accept OR reject lowest tender or any tender or all the tenders.
b) To accept any tender in full or in part.
c) To reject the tender offer not confirming to the tenders terms.
13. TERMS OF PAYMENT : Payment terms should be mentioned clearly. It is in the discretion of
the BUYER to accept, modify or reject the payment terms offered by BIDDER. Custom Duty
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exemption certificate will be provided. Vendor is allowed to split the payment in foreign
currency and in Indian Rupees (for items or services being paid, procured and provided locally).
14. In case any BIDDER is silent on any clauses mentioned in this tender document, BUYER shall
construe that the BIDDER had accepted the clauses as per the invitation to tender. No further
claim will be entertained.
15. Revision in the terms and conditions quoted in the offer will be entertained in the Technical
committee and Purchase Committee.
16. LIQUIDATED DAMAGES: Timely delivery is essence of the contract and hence if any
consignment be delayed, liquidated damages at the rate 0.5% of the price of the delayed
consignment, for each week or part whereof shall be levied and recovered subject to maximum
of 5% of total purchase order value.
17. GUARANTEE / WARRANTY : Supplier/BIDDER should give guarantee/warranty for 36
months, from the date of Installation, for the performance of the instrument. Supplier/BIDDER
should give Non comprehensive maintenance contract for 24 months, from the date of expiry of
the Warranty.
18. SUPERVISION OF ERECTION & COMMISSIONING: Successful BIDDER shall depute
concerned specialist, for supervision of erection & commissioning of the machine to be carried
out. The successful BIDDER shall make necessary arrangement at their own expenses for stay,
transport and other expenses of their specialist during their stay in Pune which also includes
imparting training to Savitribai Phule Pune University personnel.
19. TRAINING : The BIDDER shall submit training proposal to the personnel of Savitribai Phule
Pune University. General training session for staff and students should be conducted by the
BIDDER.
20. LEGAL MATTER: All Domestic and International disputes are subject to Pune Jurisdiction
Only.
21. If BIDDER is not quoting directly, name and address of agents and representatives in India
should be disclosed.
22. The BIDDER agrees that if it makes incorrect statement on this subject, BIDDER can be
disqualified from the tender process or the contract, if already awarded, can be terminated for
such reason.
23. While submitting bid, the BIDDER shall deposit an amount mentioned in tender document as
Earnest Money Deposit, with the BUYER via online payment, payable to The Finance and
Accounts Officer, Savitribai Phule Pune University.
24. The Earnest Money shall be valid up to a period of 180 days or the complete conclusion of the
contractual obligations to the complete satisfaction of both the BIDDER and the BUYER,
including warranty period, whichever is later.
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25. No interest shall be payable by the BUYER to the BIDDER on Earnest Money for the period of
its currency.
26. In case of successful BIDDER, EMD will be returned within 30 days from the date of
submission of Performance Guarantee Bond.
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ANNEXURE (A)
Technical Specifications : Tissue Culture Lab
1) Biosafety cabinet class II:
BIOSAFETY CABINET - CLASS IISPECIFICATIONS: CERTIFICATION, EN12469, EUROPE
FILTERS CERTIFIED, ULPA FILTER FILTER EFFICIENCY:>99.999% 0.3 MICRON PARTICLES,
WITH MICROPROCESSOR CONTROL, AVERAGE AIR FLOW VELOCITY-0.45M/S, AVERAGE
AIR FLOW VOLUME-365 M3/L, DOWNFLOW FILTER EFFICIENCY-99.999%, SOUND
EMISSION LESS THAN 65 DBA (WITH CERTIFICATION), ELECTRO GALVANIZED STEEL
BODY WITH EPOXY ANTIMICROBIAL POWDER COAT, MULTI-PIECE STAINLESS STEEL
WORK PLATFORM, UV ABSORBING TEMPERED GLASS, MAX SIZE- 1340 MM (LENGTH): 4
FT INTERNAL LENGTH.
Detailed specification for Class II,A2 cabinet Biosafety cabinet:
1. The Bio safety cabinet should be Class II, Type A2 cabinet. 3ft BSC cabinet is preferred.
2. The Bio safety Cabinet should be NSF or EN certified and listed.
3. The Bio safety cabinet should incorporate HEPA or ULPA filter of the class H 14 EN 1822
having efficiency of 99.995% at 0.3 µm particle size.
4. The cabinet should be provided with fixed / adjustable Height Stand, UV Light , one set of
preferably detachable arms rest and one / two electrical outlet.
5. Accessories required for using gas, vacuum etc inside the cabinet to be quoted optionally.
6. The cabinet should be provided with Microprocessor controller and large LED display for
HEPA filter loading, inflow and Down flow air velocity and hours of operation, Audible and
visual Alarms for HEPA filter failure, blower failure, airflow speed failure, Incorrect window
position.
7. The cabinet should have high energy efficient independent motor for inflow and downflow,
which automatically adjust the airflow speed without the use of a damper/ even without
maintenance adjustments. DC motors are preferred.
8. Safety Design: provision that exhaust blower on the cabinet must continue operating even when
the supply blower stops working. If the exhaust blower fails, the supply filter should also be
turned off.
9. Heat Emissions at 25ºC should be approx 0.2 KW or lesser is preferred.
10. Cabinet pressure sensor should detect pressure drop across the supply filter rather than single
point measurement across the down flow.
11. The pressure sensor must be encased to protect from temperature, humidity and other
environmental phenomena.
12. The microprocessor must display the inflow and down flow air velocities in real-time on an
LED display to ensure the user knows whether or not the cabinet is working under safe
operating conditions.
13. The front window must be a 10¿ sash opening and be made of laminated safety glass to ensure
containment.
14. All interior and exterior parts must be painted or smooth to ensure no risk of cuts to users.
15. The front of the cabinet must be angled to minimize glare & ergonomically designed.
16. The cabinet with noise level less than 63 dB(A) is prefered.
17. The interior of the front window must be accessible for cleaning without requiring the user
remove or support the window is preferred.
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18. BSC with energy consumption less than 200 watts is preferred.
19. Cabinet should be supplied with programmable UV light, to select specific exposure time from
0 to 24 hrs.
20. Cabinets with reduced energy mode is preferred, when not in use.
2) CO2 incubator (Air Jacketed):
Microprocessor controlled INCUBATOR, interior volume of 150 Ltr direct heat stackable CO2
Incubator temperature control from 4° C above ambient to 60°C, with control accuracy ±0.1°C. with
high Tem. Decontamination at 120. - It should have six-sided direct heating with fanless, gentle
convection circulation to provide stable temperature control, excellent uniformity and rapid recovery
with no over shoot. - It should have CO2 control range from 0.2 to 20% with control accuracy and
uniformity of ±0.1% and should have rapid recovery of at least 0.7% per minutes. - It should have
Infra-red (IR) CO2 sensor, sensors and alarms for both temperature and CO2, It should come with
minimum 3 adjustable height shelves & humidity reservoir (removable) to achieve at least 95% RH. - It
should have independent door heater eliminate condensation on inner door surface. - It should have
HEPA filter on CO2 inlet, It should have 25mm access port., stainless steel interior and shelves,
observation door of toughened glass door -
3) Hypoxic incubator
Chamber volume 150l, temperature control: direct heat, sensors for temperature, CO2 and O2,
sterilization cycle 145 dry or 95 humidified, humidity control and water pan, 02 control sensor 0.5 to
21%
4) Cooling benchtop centrifuge
Temp. range –9 to + 40 deg C • Short spin key • Fast cool option & stand by cooling • Facility to put
“at set rpm” function , Broad rotor and adapter selection to spin all commonly used tubes, bottles,
flasks and plates, centrifugation speed up to 20000 rpm for molecular applications in tubes from 0.2 to
100 mL, Ability to spin 15 ml and 50 ml falcon tubes upto 20000 rpm with fixed angle rotor • Adaptor
should be available for volumes from 0.2 ml to 85 ml in the Fixed angle rotor. The fixed angle rotor
should accommodate both round bottom tubes as well as falcon tubes • Rotors must be metallic Low
noise level, EASY loading/unloading of rotor, Centrifuge lid with soft-touch lid closure for stress-free
operation, aerosol-tight rotor lids and caps for fast and ergonomic lid locking, temperature range of 0
to 40 C, timer : sec,min to hrs with continuous run mode.
5) -30 freezers
Auto defrost, capacity: 240 l, cooling type: frost free, temperature range : -16 to -30 C,
baskets/shelves: 6, LED Temperature Display & Alarm system, Quick freezing function , Sealed
drawers – prevent frost loss from open door, Inner surface is made up of medical grade compression
moulded plastic, Outer body is made up of high grade polished galvanized steel, Solid state digital
temperature controller with alarms
Adjustable baskets / shelves, Low energy consumption and noise levels, Energy efficient.
6) -80 freezers:
One -80 degree upright deep freezer system with temperature control/display, temperature range: -50
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C to - 86C, Capacity cu ft/liters: 350 or more, Cryo Box Capacity 2”:230 or more, Max.shelf Weight:
120lbs/65 kg or more, Refrigeration HP: Two 1.25 HP each, Voltage:230V, 50/60 Hz. Heavy gauge,
cold rolled steel cabinets with a powder coat paint finish for a uniform exterior that resists chipping and
rust, 5” (12.7 cm) foamed-in-place polyurethane insulation, vacuum relief port for easy access after
door openings, easy to remove washable filter for protection from dust on the condenser and increasing
refrigeration performance, single hand operation with an easy-to-use padlock-compatible, ergonomic
door handle with integrated key lock, simplified installation with our new easy –roll 2” locking casters,
two/three interior doors to reduce cold air loss and improve temperature recovery after door openings,
microprocessor control and monitoring system to ensure that all controls and displays are easy to reach
and read, power management system with low voltage surge protection and buck/boost, safety-backup
systems for additional sample protection in the event of power or mechanical failure. Suitable voltage
stabilizer to be supplied to support the instrument.
7) Inverted phase contrast microscope:
Inverted Microscope with transmitted light LED illumination.
The Microscopes should include Fixed stage plate with left or right hand drive carrying standard
inserts for slide or various culture plates, flasks, hemocytometer and Petri-dishes etc. , A 4-fold
revolving nosepiece with infinity optics, tube lens, phototubes-condenser holder including collector,
aperture diaphragm and filter holder, Illuminated ON/OFF switch-Interface - power supply including
mains cable, Trinocular tube with 40-45° viewing angle, Condenser with a free working distance of
35-40 mm and a numerical aperture of 0.45 or better for Bright field Phase Contrast studies, Slider for
Phase Contrast with 4 positions, 1x Bright field and 3x light rings for condenser., Objectives Plan
Achromatic of 5x, 10x, 20x and 40x with long working distance for Bright field and Phase contrast
applications, 10X magnification eye pieces with minimum 20 mm field of view, Digital camera with
software kit:- Color digital camera for on-screen microscope image display with min resolution of
2000x1500 pixels or better 1/2" CMOS sensor progressive scan, 24 bit or higher color depth, Suitable
optical C-mount, The system should be supplied with the latest configuration Desktop which supports
the functionality of instrument
8) Fully automated inverted fluorescence microscope:
Microscope Frame: 1. Inverted microscope frame with motorized Z-Stepper (step size 0.02 or better)
should have manual access for course and fine adjustments for focus and motorized switching between
dual light path (camera 100% or eyepiece 100%) or triple light path (camera 100% or camera/eyepiece
50/50 or eyepiece 100%). 2. One fluorescence filter cube turret. 3. Two camera ports should be active
4. Touch Panel to control for the motorized parts of the microscope, Eye piece: 2 No. 10X
magnifcation, F.N 22 with focus correction, Nose piece: Motorized 6 position , Condenser: Condenser
with ~0.55 NA and ~27 mm working distance, 6 Position or better with DIC , Stage: Motorized xy-
stage with adapters for Labtek chambers, 35mm dishes and 6 well plate, Objectives: 1. Field corrected
and with relevant DIC components. 2. Apochromat 10X/0.3 or better NA 3. Apochromat 20X/0.45 or
better NA 4. Apochromat 40X/0.6 or better NA 5. Apochromat 60XOil/1.25 or better NA, Light source:
1. For bright field: 12V 100W Halogen lamp with dispersion filter, Day light filter, ND 25 and ND 6
with intensity control for Lamp. , For Fluorescence: 120 W metal halide or equivalent with ~2000hr of
lifetime. Should have intensity control and Fast shutter 3. Power supply w.r.t Indian requirements,
Fluorescence turret: Motorized Turret with 6 or higher filter cube positions, Filter cubes with
excitation, emmision and dichroics suitable for: 1. DAPI 2. FITC, GFP, Alexa 488, etc,. 3. TRITC,
RFP, Rhodamine, Alexa 561etc,. 4. CFP, Cerulean etc,. 5. YFP 6. DIC , Camera: 1. ~5MP color camera
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which can also be used as monochrome. 2. Pixel size : ~6uM 3. frame rate : 25 FPS or better at full
capacity of the chip (without binning). Quantum efficiency should be above 65% or higher, Internal
cooling to run camera for very long duration, Camera should be 12 bit or higher, Minimum thermal
and dark noise with Low exposure time
Software and computer: 1. Suitable software to control the motorized parts of the microscope to
automate the aquisition in multidimension.
2. The system should be supplied with the latest configuration Desktop which supports the
functionality of instrument
Optional 5MP Monochrome camera, 1. Pixel size : ~6uM 2. frame rate : 25 FPS or better at full
capacity of the chip (without binning). 3. Quantum efficiency should be above 65% or higher 4.
Internal cooling to run camera for very long duration. 5. Camera should be 12 bit or higher. 6.
Minimum thermal and dark noise with Low exposure time
Optional: live imaging
9) Multimode microplate reader
The instrument should be a spectral scanning multimode microplate reader capable of doing
photometry Absorbance, Fluorometric Intensity, Luminescence, Time resolved Fluorescence
System should have 1) End point, Kinetic, spectral scanning and well area scanning read methods.
2) Quadruple Monochromator based, double excitation and double emission monochromators with
wavelength range of 200-1000nm in step of 1nm for fluorescence applications.
3) double monochromators for photometric absorbance (UV-Visible) measurement. 4) xenon Flash
lamp as a light source.
5) 6 to 384 well microplate for all type of measurements.
6) should be able to read different type of 96/384 well plates covered with lid.
7) should have Photometry measurement range from 200-1000nm.
8) should have wavelength bandwidth ≤10nm for florescence and absorbance.
9) should have fluorescence detection limit of less than 1fmole/well in top read and less than
5fmole/well for bottom read for 96/384 well plates.
10) should have luminescence detection limit less than 10amol/well for glow luminescence and
15amol/well for flash luminescence.
11) should have should have on-board path length correction for direct quantification. 12). System
should have plate read time of ≤15 seconds for 96 well plates and ≤50s seconds for 384 well plates.
13) should have automatically calibrate results with different gain settings to obtain single consistent
measurement range.
14) should have Self diagnostic option and auto-calibration during the starting of the instrument as well
as during longer kinetic assays.
15) should have automatic dynamic range selection to adjust the photomultiplier tube sensitivity based
on the signal strength of the sample well.
16) should have Onboard Incubator and shaker. Incubation temperature should be up to 45 C and
Orbital/Linear shaker.
17) should have temperature safety control feature for protection against over temperature.
18) System should have Orbital Shaking with adjustable timing, speed and diameter. 19) System
should have automatic plate check mode and priming vessel check mode to prevent accidental
dispensing of reagent inside the instrument. System should have safety control on the shaking speed
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and plate format to avoid spilling of the liquid from wells.
20) System should be supplied with Analysis software with unlimited user license. System should
support Single software program should allow any number of measurement steps, different detection
modes within the program.
21) System should have different file formats during data export which includes .xlsx, .pdf, xml, and
.txt
22). System should have memory back up for measured data in case of power failure. 23) System
should be supplied all the accessories which includes fluorescence filters as a part of main offer.
24) The system should be supplied with necessary accessories required for calibration. 25) The system
should have automatic restart function in case of powers failure.
26) The system should operate at 230 volt, 50Hz.
27) The system should be supplied with the latest configuration Desktop which supports the
functionality of instrument, with a CD drive
10) Variable Water bath:
Should have a double walled construction.
The inner chamber and top lid should be made of stainless steel.
The space between the two walls should be packed with thick glass wool.
Should provide with a microprocessor based variable digital temperature controller with digital
display.
Working temperature should be from ambient to 80ºC having an accuracy of +/- 1ºC
Should have an approximate inner chamber dimension of 450mm x 300mm x 175mm.
11) Refrigerator
Frost Free, Double Door: Auto defrost to stop ice-build up
Capacity 335 L: Suitable for families with 5 or more members
Warranty: 1 year on product, 10 years on compressor
Energy Rating: 3 Star
Shelf Type: Toughened Glass
Also included in the box: User manual, Warranty card
12) Vertical Autoclave:
Capacity (Ltr.): 250 x 450 mm: 22 Ltr. , 300 x 550 mm: 40 Ltr. , 350 x 550 mm: 50 Ltr. , 400 x
600 mm: 78 Ltr. , 450 x 600 mm: 98 Ltr. , 50 x 750 mm:186 Ltr.
Material: Stainless Steel (304 Grade), 16 SWG
Hammer tone coated Steel Stainless Steel (304 Grade), 16 SWG Stainless Steel
Lid Mild Steel Plate with SS Lining
Radial Type Lid Locking System with Pedal lifting arrangement.
Basket: Stainless Steel Sheet (304)
Heaters Specially designed water heaters (ISI Marked) of suitable wattage to ensure optimum
performance and long life.
Working Temperature & Pressure:121 ºC & 15 PSI
Pressure Controller Piezostat a semi automatic electro mechanical device also know as Pressure
Control Switch. Normal Pressure Range is 5 to 20 PSI though the Autoclave is hydraulically
tested at 40 PSI.
Valves: Precision Shut off Valves for Water Drainage & Steam release.
Indicators Water Level Indicator & Pressure Gauges.
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Digital Temperature & Pressure Indicators (Optional)
Safety: SPRING LOADED SAFETY VALVE & one extra Safety Valve to discharge the steam,
if the pressure exceeds the allowed limit. the safety valves will discharge the LOW WATER
PROTECTION DEVICE to give protection to the heaters against the low water scenario under
prolonged usages. (Deluxe Model)
Gasket: Joint less Neoprin Rubber Gasket/Silicon Gasket
Electric Supply 220V +/- 10, 1 Phase.
13) Minifuge:
Max RCF 9,800 xg (100 xg increments)
Max speed 12,500 xg (100 rpm increments)
Max capacity 12 x 2mL
Rotors & adapters included
12 x 1.5/2.0mL rotor, 32 x 0.2mL rotor (singles or 4 x 8 strip), 0.2mL tube adapters (set of 12)
0.5mL tube adapters (set of 12)
Max noise level 51–53 dBA
Available timer settings 1 sec to 30 min (1 sec increments), and quick-spin
14) Vortex mixer
Speed Range 230V: 0 - 2,850 rpm,120V: 0 - 3,400 rpm
Operating Modes Touch or continuous
Ambient Operating Range +4° to 65°C
Electrical 230V~, 50 Hz or 120V~, 60 Hz
