Scribd
Upload
86 views5 pages

Stool Culture: Thiosulfate Citrate Bile Salts Sucrose Agar

This document outlines the procedure for a stool culture experiment involving the inoculation and examination of various agar plates and biochemical tests. Students are asked to identify the function of different plated media, describe any colonies observed after incubation, and record the results and interpretations of biochemical tests. The goal is to use these results to identify the probable organism(s) present in the stool sample.

Uploaded by

Dorothy Reina Monsalud
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
86 views5 pages

Stool Culture: Thiosulfate Citrate Bile Salts Sucrose Agar

This document outlines the procedure for a stool culture experiment involving the inoculation and examination of various agar plates and biochemical tests. Students are asked to identify the function of different plated media, describe any colonies observed after incubation, and record the results and interpretations of biochemical tests. The goal is to use these results to identify the probable organism(s) present in the stool sample.

Uploaded by

Dorothy Reina Monsalud
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 5

Group #

SN and Name of Members:


Section:
Stool Culture
Day 1
A. Draw the uninoculated media to be used. Identify the following plated media
according to use/function.

MacConkey Agar
Type of Medium accdg to
Function:
________________________

Hektoen Enteric Agar


Type of Medium accdg to
Function:
________________________

Eosin Methylene Blue Agar


Type of Medium accdg to
Function:
________________________

Xylose Lysine
Deoxycholate Agar
Type of Medium accdg to
Function:
________________________

Salmonella Shigella Agar


Type of Medium accdg to
Function:

____________________________
_

Thiosulfate Citrate Bile Salts


Sucrose Agar

Type of Medium accdg to


Function:
___________________________

Bismuth Sulfite Agar


Type of Medium accdg to
Function:
_____________________________

Triple Sugar
Iron Agar

Lysine Iron
Agat

Sulfide Indole Motility

Tryptone
Broth

Methyl Red
Broth

Voges
Proskauer
Broth

Simmon
Citrate Agar

Urea Broth

Day 2
Illustrate the colonies that have grown on each plated medium
EMB Agar
Description of Colonies

Sugar/s Fermented:

MacConkey Agar

Description of Colonies

Sugar/s Fermented:

HEA

Description of Colonies

Sugar/s Fermented:

XLD Agar

Description of Colonies

Sugar/s Fermented:

SSA

BSA

TCBS Agar

Description of Colonies

Sugar/s Fermented:
Description of Colonies

Sugar/s Fermented:
Description of Colonies

Sugar/s Fermented:

Day 3 (Biochemical Tests)


TSI

LIA
Interpretation

Interpretation

Day 4 (Continuation of Biochemical Tests)


Indole Test

Methyl Red Test


Result &
Interpretatio
n:

Voges Proskauer Test

Result &
Interpretatio
n:

Citrate Utilization

Result &
Interpretatio
n:

SIM

Urease
Result &
Interpretatio
n:

Probable Organism/s:

Result &
Interpretatio
n:

Result &
Interpretatio
n:

You might also like