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Anally Tical

This lesson plan outlines 8 units to teach analytical instrumentation over 52 hours. Unit 1 covers spectrophotometers including UV-Vis spectrophotometers and colorimeters. Unit 2 discusses flame photometers for clinical analysis. Unit 3 presents fluorimeters and phosphorimeters for fluorescence and phosphorescence measurements. Unit 4 introduces mass spectrometry and NMR spectroscopy. Units 5-7 cover infrared spectroscopy, electrophoresis/densitometry, and various types of chromatography. Unit 8 examines blood gas analyzers for measuring pH, pCO2 and pO2 levels. Students will learn principles and applications of these analytical instruments through lectures, readings from two textbooks and reference materials.

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51 views2 pages

Anally Tical

This lesson plan outlines 8 units to teach analytical instrumentation over 52 hours. Unit 1 covers spectrophotometers including UV-Vis spectrophotometers and colorimeters. Unit 2 discusses flame photometers for clinical analysis. Unit 3 presents fluorimeters and phosphorimeters for fluorescence and phosphorescence measurements. Unit 4 introduces mass spectrometry and NMR spectroscopy. Units 5-7 cover infrared spectroscopy, electrophoresis/densitometry, and various types of chromatography. Unit 8 examines blood gas analyzers for measuring pH, pCO2 and pO2 levels. Students will learn principles and applications of these analytical instruments through lectures, readings from two textbooks and reference materials.

Uploaded by

chetan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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LESSON PLAN

ANALYTICAL INSTRUMENTATION

Sub Code : 10 EI 65 IA Marks : 25 Hrs/Week : 04


Exam Hours : 03 Total Hrs : 52 Exam Marks : 100
PARTA

Actual hour Hours


unit Topics
ofclasses taken

1 VISIBLE ULTRAVIOLET SPECTROPHOTOMETERS:


Electromagnetic radiation, Beer Lambert law, absorption instruments,
7 8
colorimeters, spectrophotometers, sources of error in spectrophotometers,
measurements, calibration.
2 FLAME PHOTOMETERS: Principle of flame photometers, constructional
details of flame photometers, clinical flame photometers, accessories of flame 7 5
photometers, interference in flame photometry and determinations.
3 FLUORIMETERS & PHOSPHORIMETERS: Principle of fluorescence,
measurement of fluorescence, spectrofluorimeters, microprocessor based 6 5
spectrofluorometer, Measurement of phosphorescence.
4 MASS SPECTROMETER & NMR SPECTROMETER: Basic mass
spectrometer, types of mass spectrometer, components of mass spectrometer,
resolution and applications. Principle of NMR, constructional details, 6 7
sensitivity enhancement for analytical NMR spectroscopy, use of computers
with NMR spectrometers.
PART B
5 INFRARED SPECTROPHOTOMETER: Infrared spectroscopy, Basic
components of infrared spectrophotometer, Types of IR spectrophotometer,
7 8
Fourier transform IR spectroscopy. Thermo-analytical Method:
Thermogravimetric analysis (TGA), Differential Thermal analysis (DTA).
6 ELECTROPHORESIS AND DENSITOMETERS: Basic Electrophoresis,
Electrophoresis techniques, paper Electrophoresis, Electrophoresis apparatus,
7 6
spectrodensitometer, microprocessor based densitometer,
microelectrophoresis.
7 CHROMATOGRAPHY: Gas chromatograph basic concepts, parts of gas
chromatograph, Method of peak areas, Liquid chromatography, basic concepts,
6 6
types of liquid chromatography, liquid chromatograph, HPLC principle and
block diagram description.
8 BLOOD GAS ANALYZER: Principle of pH measurement, electrode for pH
measurement, Blood pH measurement, measurement of Blood pCO 2 ,
measurement of Blood pO complete Blood gas analyzer, commercially 6 4
available blood gas analyzers.
TEXT BOOK:
1. Hand Book of Analytical Instruments - By R.S. Khandpur, TMH Publications, 1 Edn. 1989.

REFERENCE BOOKS:
1. Instrumental methods of analysis by H.H Willard, L.L. Meritt & A. Dean, CBS Publications
7 St Edn., 1988
2. Principles of Instrumental analysis by S.J. Holler & T. A. Nilman Saunders college
publications, 5
Edn, 1998.

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