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Open Access Publications from the University of California

Scholarly Works (20 results)

  • Thesis
  • Peer Reviewed
UCLA Electronic Theses and Dissertations (2020)

This research examines the formation of a Taiwanese American identity from a transnational perspective, filling a scholarly gap in the understanding of intraethnic diversity. Before the surge of Taiwanese immigration to the United States since the 1970s, Taiwanese Americans and Chinese Americans were often perceived as a single ethnonational group, and the two identities conflated. As they have established themselves as a distinct community, Taiwanese Americans, the children of Taiwanese immigrants in particular, have proactively constructed a distinct identity separate from Chinese Americans. The lifting of martial law in 1987 and democratization in Taiwan in the 1990s allowed Taiwanese and the Taiwanese diaspora to publicly and vocally oppose the Kuomintang (KMT) nationalist party and its one-party rule in Taiwan, giving rise to increasing public support for Taiwanese nationalism and Taiwanization efforts, encouraging Taiwanese Americans to fully embrace the Taiwanese independence movement, create and reinforce a Taiwanese American identity, and call for recognition of Taiwan in international organizations. I argue that the Taiwanese American identity is formed through the disidentification from Chinese American community, construction of socioeconomic support networks, engagement with transnational political activism, and appropriation of Taiwanese Indigenous symbols.

    Cover page: Strait to the Point: A Transnational Analysis of the Formation of a Taiwanese American Identity
    • Article
    • Peer Reviewed
    Program on International Migration (2021)
      Cover page: Understanding Intraethnic Diversity: The Formation of a Taiwanese American Identity
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      • Peer Reviewed
      LBL Publications (2021)
      To support an ever-increasing population, modern agriculture faces numerous challenges that pose major threats to global food and energy security. Plant-associated microbes, with their many plant growth-promoting (PGP) traits, have enormous potential in helping to solve these challenges. However, the results of their use in agriculture have been variable, probably because of poor colonization. Phytomicrobiome engineering is an emerging field of synthetic biology that may offer ways to alleviate this limitation. This review highlights recent advances in both bottom-up and top-down approaches to engineering non-model bacteria and microbiomes to promote beneficial plant-microbe interactions, as well as advances in strategies to evaluate these interactions. Biosafety, biosecurity, and biocontainment strategies to address the environmental concerns associated with field use of synthetic microbes are also discussed.
        Cover page: Microbiome Engineering: Synthetic Biology of Plant-Associated Microbiomes in Sustainable Agriculture
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        • Peer Reviewed
        UC Davis Previously Published Works (2012)
        AbstractBackgroundOptimal nutrition for lactating mothers is importance for mother and infants’ health and well-being. We determined the nutrient intake and dietary changes during the first 3-month of lactation, and its potential effect on health and disease risk.MethodPersonal interviews were conducted to collect a 24h diet recall questionnaire from 199 healthy lactating women in the postpartum days 2, 7, 30, 90 and healthy 58 non-pregnant women served as the controls.ResultsWe found in lactating women (1) the mean daily energy and carbohydrate intake was lower than that of the Chinese Recommended Nutrient Intake (RNI, 2600 Kcal, 357.5 ~ 422.5g) by 11% ~ 17% and 33% ~ 49%, respectively; (2) the fat intake increased from 3% to 13%, which was 9 ~ 77% higher than the RNI (57 ~ 86.7g); (3) the protein intake exceeded the RNI of 85g by 32 ~ 53%; (4) the total calories consumed from carbohydrate (39%-44%), fat (34% ~ 42%) and protein (20%-23%) failed to meet Chinese RNI (5) the intake of vitamin C, B1, folate, zinc, dietary fiber, and calcium was 5% ~ 73% lower than the RNI while vitamin B2, B3, E, iron and selenium intake was 20% to 3 times higher than the RNI. Nutrient intake in the control group was lower for all nutrients than the recommended RNI.ConclusionLactating women on a self-selected diet did not meet the Chinese RNI for many important micronutrients, which may influence the nutritional composition of breast milk and thus impact the potential health of mothers and infants. RNI should consider the regional dietary habits and culture. A single national RNI is not applicable for all of China. Nutritional education into the community is needed.
          Cover page: Evaluation of dietary intake of lactating women in China and its potential impact on the health of mothers and infants
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          UC Davis Previously Published Works (2017)

          Background

          Musculoskeletal injuries are the most common complaint in active populations. More than 50% of all injuries in sports can be classified as sprains, strains, ruptures, or breaks of musculoskeletal tissues. Nutritional and/or exercise interventions that increase collagen synthesis and strengthen these tissues could have an important effect on injury rates.

          Objective

          This study was designed to determine whether gelatin supplementation could increase collagen synthesis.

          Design

          Eight healthy male subjects completed a randomized, double-blinded, crossover-design study in which they consumed either 5 or 15 g of vitamin C-enriched gelatin or a placebo control. After the initial drink, blood was taken every 30 min to determine amino acid content in the blood. A larger blood sample was taken before and 1 h after consumption of gelatin for treatment of engineered ligaments. One hour after the initial supplement, the subjects completed 6 min of rope-skipping to stimulate collagen synthesis. This pattern of supplementation was repeated 3 times/d with ≥6 h between exercise bouts for 3 d. Blood was drawn before and 4, 24, 48, and 72 h after the first exercise bout for determination of amino-terminal propeptide of collagen I content.

          Results

          Supplementation with increasing amounts of gelatin increased circulating glycine, proline, hydroxyproline, and hydroxylysine, peaking 1 h after the supplement was given. Engineered ligaments treated for 6 d with serum from samples collected before or 1 h after subjects consumed a placebo or 5 or 15 g gelatin showed increased collagen content and improved mechanics. Subjects who took 15 g gelatin 1 h before exercise showed double the amino-terminal propeptide of collagen I in their blood, indicating increased collagen synthesis.

          Conclusion

          These data suggest that adding gelatin to an intermittent exercise program improves collagen synthesis and could play a beneficial role in injury prevention and tissue repair. This trial was registered at the Australian New Zealand Clinical Trials Registry as ACTRN12616001092482.
            Cover page: Vitamin C–enriched gelatin supplementation before intermittent activity augments collagen synthesis 1 , 2
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            • Peer Reviewed
            Western Journal of Emergency Medicine: Integrating Emergency Care with Population Health, Volume 26, Issue 4 (2025)

            Introduction: Prolonged emergency department (ED) waiting times for STAT spine magnetic resonance imaging (MRI) in the ED can expose patients to hospital-acquired infections and increase the workload in the ED, further impacting healthcare quality. In this study we aimed to characterize emergent spine MRI frequency and positivity in the ED, and its impact on ED length of stay (LOS), admission rates, and the necessity for surgical interventions.

            Methods: We performed a retrospective chart review of a consecutive group of patients who had emergent spine MRI (cervical, thoracic, lumbar) ordered from the EDs at four hospitals from January 1, 2017-December 31,2022 were included for traumatic and atraumatic patients. We recorded patient demographics, time metrics, discharge status, and surgical interventions within seven days (for those who were hospitalized during the ED encounter). Spine MRI reports were reviewed and categorized, with positive cases defined as severe spinal canal stenosis regardless of cause and/or fracture. We used descriptive statistics to assess the positivity rate for emergent spine MRIs as well as the LOS, rate of surgery, and rate of admission for patients getting emergent spine MRIs.

            Results: A total of 689 spine MRI of 889,527 ED visits (0.1%) were included. Patients’ mean age was 51.3 ±17.1 years, and 59.5% were female. Discharge rate was 93.9%, 3.3% were admitted, 1.7% left against medical advice, and 1.0% were transferred to other facilities. The overall spine MRI positivity rate was 18.9% (130). Moreover, the median (IQR) time from imaging order placement to imaging completion was 2.6 (1.8 - 3.7) hours, while the time from imaging completion to final report availability was 1.5 (0.4 - 13.9) hours. The median ED LOS was 7.4 (5.7 - 9.5) hours. Of 23 hospitalized patients, 17 (73.9%) required surgical intervention. Positive cases had significantly higher ED LOS compared to negative cases (8.1 vs 7.2, respectively; P < .001).

            Conclusion: The positivity rate for ED spine MRI in this study was 18.9%. Of the positive cases, 17.7% underwent hospitalization, with 13.1% requiring emergent surgery. Considering high costs in both time and resource utilization, further research is needed to optimize the triage process for patients requiring emergent spine MRI.

            • 1 supplemental ZIP
            Cover page: Utility of Emergent Spine MRI in the Emergency Department
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            • Peer Reviewed
            UC Irvine Previously Published Works (2013)
            Dysfunction of circadian clocks exacerbates various diseases, in part likely due to impaired stress resistance. It is unclear how circadian clock system responds toward critical stresses, to evoke life-protective adaptation. We identified a reactive oxygen species (ROS), H2O2 -responsive circadian pathway in mammals. Near-lethal doses of ROS-induced critical oxidative stress (cOS) at the branch point of life and death resets circadian clocks, synergistically evoking protective responses for cell survival. The cOS-triggered clock resetting and pro-survival responses are mediated by transcription factor, central clock-regulatory BMAL1 and heat shock stress-responsive (HSR) HSF1. Casein kinase II (CK2) -mediated phosphorylation regulates dimerization and function of BMAL1 and HSF1 to control the cOS-evoked responses. The core cOS-responsive transcriptome includes CK2-regulated crosstalk between the circadian, HSR, NF-kappa-B-mediated anti-apoptotic, and Nrf2-mediated anti-oxidant pathways. This novel circadian-adaptive signaling system likely plays fundamental protective roles in various ROS-inducible disorders, diseases, and death.
              Cover page: ROS stress resets circadian clocks to coordinate pro-survival signals.
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              LBL Publications (2021)
              Fabricated ecosystems (EcoFABs) offer an innovative approach to in situ examination of microbial establishment patterns around plant roots using nondestructive, high-resolution microscopy. Previously high-resolution imaging was challenging because the roots were not constrained to a fixed distance from the objective. Here, we describe a new 'Imaging EcoFAB' and the use of this device to image the entire root system of growing Brachypodium distachyon at high resolutions (20×, 40×) over a 3-week period. The device is capable of investigating root-microbe interactions of multimember communities. We examined nine strains of Pseudomonas simiae with different fluorescent constructs to B. distachyon and individual cells on root hairs were visible. Succession in the rhizosphere using two different strains of P. simiae was examined, where the second addition was shown to be able to establish in the root tissue. The device was suitable for imaging with different solid media at high magnification, allowing for the imaging of fungal establishment in the rhizosphere. Overall, the Imaging EcoFAB could improve our ability to investigate the spatiotemporal dynamics of the rhizosphere, including studies of fluorescently-tagged, multimember, synthetic communities.
                Cover page: Microfabrication of a Chamber for High-Resolution, In Situ Imaging of the Whole Root for Plant–Microbe Interactions
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                UC San Francisco Previously Published Works (2013)

                Purpose

                PARP1/2 inhibitors are a class of anticancer agents that target tumor-specific defects in DNA repair. Here, we describe BMN 673, a novel, highly potent PARP1/2 inhibitor with favorable metabolic stability, oral bioavailability, and pharmacokinetic properties.

                Experimental design

                Potency and selectivity of BMN 673 was determined by biochemical assays. Anticancer activity either as a single-agent or in combination with other antitumor agents was evaluated both in vitro and in xenograft cancer models.

                Results

                BMN 673 is a potent PARP1/2 inhibitor (PARP1 IC50 = 0.57 nmol/L), but it does not inhibit other enzymes that we have tested. BMN 673 exhibits selective antitumor cytotoxicity and elicits DNA repair biomarkers at much lower concentrations than earlier generation PARP1/2 inhibitors (such as olaparib, rucaparib, and veliparib). In vitro, BMN 673 selectively targeted tumor cells with BRCA1, BRCA2, or PTEN gene defects with 20- to more than 200-fold greater potency than existing PARP1/2 inhibitors. BMN 673 is readily orally bioavailable, with more than 40% absolute oral bioavailability in rats when dosed in carboxylmethyl cellulose. Oral administration of BMN 673 elicited remarkable antitumor activity in vivo; xenografted tumors that carry defects in DNA repair due to BRCA mutations or PTEN deficiency were profoundly sensitive to oral BMN 673 treatment at well-tolerated doses in mice. Synergistic or additive antitumor effects were also found when BMN 673 was combined with temozolomide, SN38, or platinum drugs.

                Conclusion

                BMN 673 is currently in early-phase clinical development and represents a promising PARP1/2 inhibitor with potentially advantageous features in its drug class.
                  Cover page: BMN 673, a Novel and Highly Potent PARP1/2 Inhibitor for the Treatment of Human Cancers with DNA Repair Deficiency
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                  UC Irvine Previously Published Works (2016)

                  Objective

                  To investigate the association between periventricular white mater hyperintensities (PVWMH) and biomarkers of elevated cerebral β-amyloid (Aβ) in the Alzheimer's Disease Neuroimaging Initiative, a large prospective multicenter observational study.

                  Methods

                  The burden of frontal, parietal, and occipital PVWMH on 3T fluid-attenuated inversion recovery MRI was evaluated in 698 cognitively normal participants and participants with mild cognitive impairment (MCI) using a novel semiquantitative visual rating scale. Results were correlated with CSF-Aβ, florbetapir-PET, and fluorodeoxyglucose (FDG)-PET.

                  Results

                  Increased burden of parietal, occipital, and frontal PVWMH was associated with elevated cerebral amyloid evidenced by high florbetapir-PET signal (p < 0.01) and low CSF-Aβ (p < 0.01). In logistic regression models, including PVWMH, age, sex, APOE status, vascular risk factors, pulse pressure, vascular secondary prevention medications, education, ethnicity, and race, parietal, occipital, and frontal PVWMH burden was independently associated with high florbetapir-PET uptake (p < 0.05). In a similar logistic regression model, parietal and occipital (p < 0.05) but not frontal (p = 0.05) PVWMH were independently associated with CSF-Aβ. Weaker associations were found between parieto-occipital PVWMH and elevated CSF-tau (p < 0.05) and occipital PVWMH and elevated CSF-phospho-tau (p < 0.05). PVWMH were associated with cerebral hypometabolism on FDG-PET independent of CSF-Aβ levels (p < 0.05). Absolute and consistency of agreement intraclass correlation coefficients were, respectively, 0.83 and 0.83 for frontal, 0.78 and 0.8 for parietal, and 0.45 and 0.75 for occipital PVWMH measurements.

                  Conclusions

                  Increased PVWMH were associated with elevated cerebral amyloid independent of potential confounders such as age, APOE genotype, and vascular risk factors. The mechanisms underlying the association between PVWMH and cerebral amyloid remain to be clarified.
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