Bugs

Those are real bees featured in the episode. Jensen Ackles was the first to get stung which amused Jared Padalecki enormously. Ackles got his revenge shortly after when Padalecki sat down on a bee and was stung on his rear.

When Sam and Dean arrive at the Pike's house to warn them, Sam says that it is 12 a.m.. Right after they get attacked by bees and flee to the house's attic. The bees get in and attack everyone. And then they see the sun rise and the curse is over. In the story only a couple minutes pass between entering the house and the end of the curse. And no indication is made to more time passing. But around the spring equinox the sun rises about 7:30 a.m. in Oklahoma. Also the next day no one has any bee-stings on their faces, necks or hands (the parts that would have been exposed to the bees).

Referenced in Supernatural: Don't Call Me Shurley (2016)