CL2008002288A1 - Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, a terminator and a report or selection reporter gene, flanked by a fragment that allows its insertion; method of transformation of organisms that generates homozygous organisms, which uses said nucleotide structure - Google Patents

Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, a terminator and a report or selection reporter gene, flanked by a fragment that allows its insertion; method of transformation of organisms that generates homozygous organisms, which uses said nucleotide structure

Info

Publication number
CL2008002288A1
CL2008002288A1 CL2008002288A CL2008002288A CL2008002288A1 CL 2008002288 A1 CL2008002288 A1 CL 2008002288A1 CL 2008002288 A CL2008002288 A CL 2008002288A CL 2008002288 A CL2008002288 A CL 2008002288A CL 2008002288 A1 CL2008002288 A1 CL 2008002288A1
Authority
CL
Chile
Prior art keywords
organisms
selection
nucleotide structure
terminator
promoter
Prior art date
Application number
CL2008002288A
Other languages
Spanish (es)
Inventor
Guzman Victor Cifuentes
Oyarzun Mauricio Alejandro Niklitschek
Caldera Andres Esteban Marcoleta
Original Assignee
Univ Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Chile filed Critical Univ Chile
Priority to CL2008002288A priority Critical patent/CL2008002288A1/en
Priority to PCT/CL2009/000006 priority patent/WO2010012118A1/en
Publication of CL2008002288A1 publication Critical patent/CL2008002288A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Estructura nucleotídica que comprende al menos un casete de selección y/o expresión con un promotor, un terminador y ungen reportero de selección o expresión; método de transformación de organismos de forma estable y homogénea generando organismos homocigotos para la transformación inicial.Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, terminator and anoint selection reporter or expression; method of transforming organisms in a stable and homogeneous way generating homozygous organisms for the initial transformation.

CL2008002288A 2008-08-01 2008-08-01 Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, a terminator and a report or selection reporter gene, flanked by a fragment that allows its insertion; method of transformation of organisms that generates homozygous organisms, which uses said nucleotide structure CL2008002288A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CL2008002288A CL2008002288A1 (en) 2008-08-01 2008-08-01 Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, a terminator and a report or selection reporter gene, flanked by a fragment that allows its insertion; method of transformation of organisms that generates homozygous organisms, which uses said nucleotide structure
PCT/CL2009/000006 WO2010012118A1 (en) 2008-08-01 2009-07-31 X. dendrorhous gene, method and nucleotide structure that can be used for the specific and selective generation of transformants, suitable for the genetic modification and evaluation of organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2008002288A CL2008002288A1 (en) 2008-08-01 2008-08-01 Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, a terminator and a report or selection reporter gene, flanked by a fragment that allows its insertion; method of transformation of organisms that generates homozygous organisms, which uses said nucleotide structure

Publications (1)

Publication Number Publication Date
CL2008002288A1 true CL2008002288A1 (en) 2010-06-11

Family

ID=41609902

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2008002288A CL2008002288A1 (en) 2008-08-01 2008-08-01 Nucleotide structure comprising at least one selection and / or expression cassette with a promoter, a terminator and a report or selection reporter gene, flanked by a fragment that allows its insertion; method of transformation of organisms that generates homozygous organisms, which uses said nucleotide structure

Country Status (2)

Country Link
CL (1) CL2008002288A1 (en)
WO (1) WO2010012118A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019009402A1 (en) 2017-07-06 2019-01-10 浜松ホトニクス株式会社 Optical device

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4796786B2 (en) * 2005-04-28 2011-10-19 富士フイルム株式会社 A vector capable of transforming Labyrinthula

Also Published As

Publication number Publication date
WO2010012118A1 (en) 2010-02-04

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