Direct in vivo investigation of mammalian metabolism is complicated by the distinct
metabolic functions of different tissues. We present a computational method that successfully …

This paper presents a new method, steady‐state regulatory flux balance analysis (SR‐FBA),
for predicting gene expression and metabolic fluxes in a large‐scale integrated metabolic–…

In metabolism, available free energy is limited and must be divided across pathway steps to
maintain a negative ΔG throughout. For each reaction, ΔG is log proportional both to a …

The inability to inspect metabolic activities within subcellular compartments has been a
major barrier to our understanding of eukaryotic cell metabolism. Here, we describe a spatial-…

Predicting the metabolic state of an organism after a gene knockout is a challenging task,
because the regulatory system governs a series of transient metabolic changes that converge …

ATP is the dominant energy source in animals for mechanical and electrical work (for example,
muscle contraction or neuronal firing). For chemical work, there is an equally important …

The Warburg effect - a classical hallmark of cancer metabolism - is a counter-intuitive
phenomenon in which rapidly proliferating cancer cells resort to inefficient ATP production via …

A fundamental challenge in human health is the identification of disease-causing genes.
Recently, several studies have tackled this challenge via a network-based approach, motivated …

In response to tenacious stress signals, such as the unscheduled activation of oncogenes,
cells can mobilize tumour suppressor networks to avert the hazard of malignant transformation…

The interest in studying metabolic alterations in cancer and their potential role as novel targets
for therapy has been rejuvenated in recent years. Here, we report the development of the …