Why do seemingly identical cells respond differently to a drug? To address this, we studied
the dynamics and variability of the protein response of human cancer cells to a …

Protein expression is a stochastic process that leads to phenotypic variation among cells 1 ,
2 , 3 , 4 , 5 , 6 . The cell–cell distribution of protein levels in microorganisms has been well …

Cells remove proteins by two processes: degradation and dilution due to cell growth. The
balance between these basic processes is poorly understood. We addressed this by …

Endogenous tissue inhibitors of metalloproteinases (TIMPs) have key roles in regulating
physiological and pathological cellular processes 1 , 2 , 3 . Imitating the inhibitory molecular …

The cell cycle is a temporal program that regulates DNA synthesis and cell division. When
we compared the codon usage of cell cycle‐regulated genes with that of other genes, we …

We examined cell cycle–dependent changes in the proteome of human cells by systematically
measuring protein dynamics in individual living cells. We used time-lapse microscopy to …

Drugs and drug combinations have complex biological effects on cells and organisms. Little
is known about how drugs affect protein dynamics that determine these effects. Here, we …

The relationships between Septoria leaf blotch severity and plant stature, and maturity class
were investigated in parental, F, and F populations, and selected lines derived from crosses …

A current challenge in biology is to understand the dynamics of protein circuits in living human
cells. Can one define and test equations for the dynamics and variability of a protein over …

We present a protocol to tag proteins expressed from their endogenous chromosomal locations
in individual mammalian cells using central dogma tagging. The protocol can be used to …