Kostunolid sintaza
| Kostunolid sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.120 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Kostunolid sintaza (EC 1.14.13.120, kostunolidna sintaza) je enzim sa sistematskim imenom germakra-1(10),4,11(13)-trien-12-oat,NADPH:kiseonik oksidoreduktaza (6alfa-hidroksilacija).[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
- germakra-1(10),4,11(13)-trien-12-oat + NADPH + H+ + O2 (+)-kostunolid + NADP+ + 2H2O
A heme-tiolate protein (P-450). Ovaj enzim hidroksiliše ugljenik C-6 germakra-1(10),4,11(13)-trien-12-oata i daje 6alfa-hidroksigermakra-1(10),4,11(13)-trien-12-oat, koji se spontano ciklizuje i formira laktonski prsten.
Reference
[уреди | уреди извор]- ^ de Kraker, J.W., Franssen, M.C., Joerink, M., de Groot, A. and Bouwmeester, H.J. (2002). „Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome p450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory”. Plant Physiol. 129: 257—268. PMID 12011356.
Literatura
[уреди | уреди извор]- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
[уреди | уреди извор]- Costunolide+synthase на US National Library of Medicine Medical Subject Headings (MeSH)