SEARCHING OF ANTIHYPERTENSIVE ACTIVE COMPOUND FROM ETHYL ACETATE

SUBFRACTION OF ROSELLE (Hibiscus sabdariffa L.) PETALS

Yasmiwar Susilawati1, Santi Perawati1, Sri Adi Sumiwi2, Ida Musfiroh3, Rini Hendriani2
1Department of Biological Pharmacy, Faculty of Pharmacy,
2Department of Pharmacology and Toxicology, Faculty of Pharmacy,
3Department of Analysis of Pharmacy and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21 Jatinagor 45363

West Java Indonesia

e-mail address of corresponding author: yasmiwar@unpad.ac.id

Background and Objectives Method and Result Conclusion

Hypertension is a disorder characterized by an increase in The Hs-II-B subfraction showed the highest
systolic and or diastolic blood pressure above normal limits. The antihypertensive activity with inhibition of systolic and
exact limits of this disorder are uncertain because they differ diastolic was 20.08% and 19.18 % while Hs-II-A
according to age and sex (Dipiro et al., 2011). Hypertension is a subfraction was 6.21% and 9.9%, and Hs-II-C
condition in which a systolic blood pressure greater than 140 subfraction was 3.81% and 2.96% respectively.
mmHg is accompanied by a rise in diastolic blood pressure
greater than 90mmHg (JNC, 2003). Treatment of hypertensive Reference
disease in general requires a long period of time and at risk to Dipiro, JT., Talbert, RL., Yee, GC., Matzke, GR., Wells, BG., L,
Michael, Posey. 2011. Pharmacoteraphy: A
other organs, especially liver and kidney (Dipiro et al., 2011). Pathophysiologic Approach 8th Edition. USA: MC Graw
The usage of antihypertensive plants for long periods is Hill Companies. 103.
recommended because the side effects are relatively low, so its Joint National Committee on Prevention, Detection, Evaluation,
use is still applied today (Joshi et al., 2012). One of the of plants and Treatment of High Blood Pressure (JNC). 2003.
that has been known as anti- hypertension is roselle (Hibiscus Seventh Report of the Joint National Committee on
Prevention, Detection, Evaluation, and Treatment of High
sabdariffa L.), that belongs to Malvaceae family (Kumar et al., Blood Pressure (JNC 7). JAMA. Vol. 289 (19): 2560-72.
2011; Talha et al., 2011). Based on research of Puspitasari, et al. Joshi, UH., Ganatra, TH., Bhalodiya, PN., Desai, TR., and Tirgar,
(2009), fractions of roselle ethanol extract can be lowering blood PR. 2012. Comparative Review on Harmless Herbs with
pressure of male white rat induced by adrenaline. The study Allopathic Remedies As Anti-hypertensive. Res.J. Phar,
showed that ethyl acetate fraction (Hs-II) has better Figure A B C Bio & Chem. Vol. 3 (2): 673-678.
1 TLC of
Kumar, VR., Kandagatla, K., and Shrutika, Chillal. 2011. Review:
antihypertensive activity than n-hexane fraction (Hs-I) and combined fraction Figure 2. Decrease of systolic Figure 3. Decrease of diastolic
Signature on Medicinal Plants Used In Hypertension. J.
of KCV yield of blood pressure of Hs-II-A, Hs-II-B, blood pressure of Hs-II-A, Hs-II-B,
ethanol (Hs-III). roselle petals. and Hs-II-C subfraction at 15, 30 and Hs-II-C subfraction at 15, 30 Phar & Tech. Vol.3 (3) : 1333-1355.
and 60 minutes. and 60 minutes.
Note: Puspitasari, R.M., Sumiwi, S.A., dan Yasmiwar, S. 2009. Aktivitas
Silent Phase : Silica Gel 60 F254
Motion Phase : Toluene: MTC: EtOAc (1: 3: 1)
Note: antihipertensi ekstrak n-heksan, etil asetat, dan etanol
K+ : Positive control group (captopril 2.25mg/kg bw)
Detection : UV rays 366 nm
Hs-II-A: group of etyhl acetate subfractions A (7.2 mg/kg bw) kelopak bunga rosela (Hibiscus sabdariffa L.) pada
A. VLC fraction (Hs-II-A)
B. VLC fraction (Hs-II-B) Hs-II-B: group of etyhl acetate subfractions B (7.2 mg/kg bw) tikus putih jantan. Skripsi Unpad: Bandung.
C. VLC fraction (Hs-II-C) Hs-II-C: group of etyhl acetate subfractions C (7.2 mg/kg bw)