Technical Manual

P/n: RAA006BEN
HAN 677A

Explore the future

 Instrument Technical Manual Update
RAM225AEN

Micros 45, abc Vet

abc Vet specific informations

Please, take note of the modifications on next pages. Please, cross out the
appropriate sections in the technical manual prior to inserting this adden-
dum at the beginning of the manual.

Date: 22/4/08
FORM 0860 - rev 1
Micros 45 / abc Vet technical manual RAM225AEN

Micros 45 / abc Vet technical manual

Tab.1-1: Concerned sections of the manual

Part number Section Page Paragraph Date
RAA006AH Addendum All abc Vet specific informations 03/03/08

1. General information
Technical informations related to maintenance and adjustments of the abc Vet instrument are not specified into
the Micros 45 technical manual. The following addendum summarizes the necessary informations.

2. abc Vet specific uses

2.1. Instrument models

abc Vet is only available in opent tube mode with reagent pack.

2.2. Software versions

The current software version is V2.61.

In 16 parameters, from software version V2.41, use only the LMGE reagent pack.

2.3. Parameters

From software version V2.6.1, abc Vet is available in 8 paramètres or 16 paramètres (with LMGE reagent pack) on
all species.

2.4. Smart cards

3 types of smart cards are available :

◆ Smart card species (dog, cat, horse, etc...)

◆ Smart card contrôl (this card has no memory and does not contain any target values)
◆ Smart card memory (identical to Micros 45)

Smart card «Human» does not exist.

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Micros 45 / abc Vet technical manual RAM225AEN

The smart card species manages :

◆ The animal type

◆ The sampled volume and the lyse volume
◆ The correction coefficients for calibration (according to the sampled volume)
◆ Coincidence correction coefficient values
◆ Upper and lower limits for each parameter
◆ PLT and WBC curve thresholds
◆ Values (in %) for SCL, SCH, MIC, EOS flags
◆ Values ’in %) for ACG1 and AG2 flags for «cat» smart card only
◆ DIL flag limits for WBC, RBC, PLT, HGB, HCT parameters

2.5. Reagents

Exclusive use of the pack LMGE.

2.6. Quality control

There is no quality control option. The smart card «QC» is similar to a species smart card, it contains only the
correct thresholds for running control blood.

2.7. Calibration

Calibration is carried out in the same way than for Micros 45 wih exclusive use of Minocal.

2.8. LMG curve thresholds

Thresholds of the LMG curve are fixed. They do not vary according to temperature like on Micros 45.

2.9. Flags

L1, M2, G1, G2 and G3 flags are not managed by abc Vet.
AG1 and AG2 for «cat» species allow the user to determine the level of cold agglutinins or platelet aggregates on
the WBC count (level 1 : AG1, level 2 : AG2). It is recommended to perform a manual count on a blood smear when
these alarms occur.

There is no suspicion nor reject on WBC count when AG1 and AG2 occur.

2.10. abc Vet startup

abc Vet is setup in factory with the «dog» species. If the instrument is turned ON when there is no smart card into
the reader, abc Vet will be setup with the latest species specified. When the latest smart card used is a QC smart
card, the instrument will be setup with the «dog» species.

◆ On software version lower than V2.41, once instrument has been set up with the animal species, it is not nec-
essary to leave the card in place (to install a memory card for example).

◆ From software version V2.41, before each cycle, abc Vet enquires the smart card into the reader and affects the
corresponding parameters.

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Micros 45 / abc Vet technical manual RAM225AEN

2.11. User manuals

Only 2 abc Vet user manuals are availables :

◆ RAB015BEN : english user manual

◆ RAB015BFR : french user manual

These 2 manuals includes the addendum RAM178A concerning the latest software version
V2.61.

3. abc Vet technical informations

3.1. WBC and RBC threshold adjustments

WBC and RBC thresholds are modified on the mother board :

◆ RBC vet threshold is adjusted to 350mV +/- 5mV instead of 400mV on Micros 45
◆ WBC vet threshold is adjusted to 826mV +/- 5mV insead of 280mV on Micros 45

The WBC volume adjustment on latex is given with the specific latex kit (vial + technical note).

4. Reminder on the abc Vet technical notes

RAH569DA : abc Vet 8P to 16P upgrade
RAH601AA : software version V2.11
RAH607AA : part number of the mother boards
RAH634AA : software version V2.21
RAH649AA : WBC threshold adjustment
RAH728AA : «cat» species smart card
RAH760AA : software version V2.45
RAN074BA : software version V2.6.1
RAN152BA : OKI4350 printer

5. Technical manual
The Micros 45 technical manual RAA006AF becomes Micros 45/abc Vet technical manual RAA006BEN.

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MICROS 45 / abc Vet

Technical Manual

P/n: RAA006BEN
MICROS 45 TECHNICAL MANUAL RAA006BEN

Introduction

1. Revisions

Table 1: Table of Revisions

Index Technical Note Revision Section Date

AA Creation All 30/09/94
AB RAH154AA Update V1.2 software version all 13/03/95
AC RAH229AA Update V2.03 software version RAS091/121 15/11/95
AD RAH262AA RAS134A & miscelaneous corrections 19/02/96
AE RAH326AA Update V2.2 software version all 04/03/97
AF RAH504AA Update all sections all 09/03/98
BEN RAN337A Addendum ABC Vet RAM225AEN 11/03/08

This document applies to the latest higher software version.

When a subsequent software version changes the information in this document, a new issue will be
released.

1.1. Notice of liability

The information in this manual is distributed on an «As Is» basis, without warranty. While every precau-
tion has been taken in the preparation of this manual, Horiba ABX will not assume any liability to any per-
sons or entities with respect to loss or damage caused or alleged to be caused directly or indirectly by
not following the instructions contained in this manual or by using the computer software and hardware
products described herein in a manner inconsistent with our product labeling.

1.2. Trademarks

Other product names mentioned within this publication may be trademarks or registered trademarks of
other companies.

1.3. Copyright® 2004 by Horiba ABX

All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of
Horiba ABX.

Horiba ABX
Parc Euromédecine
Rue du caducée
B.P. 7290
34184 MONTPELLIER Cedex 4 - FRANCE
Tel: (33) (0)4 67 14 15 16
Fax: (33) (0)4 67 14 15 17

2/2
 SECTION 1

1. INTRODUCTION ............................................................................................................................2
1.1. Preliminaries ...................................................................................................................2
1.2. Description of the manual ...............................................................................................2
 SECTION 2

2. HYDRAULIC AND PNEUMATIC PRINCIPLES ..............................................................................2

2.1. Introduction......................................................................................................................2
2.2. Hydraulic parts ................................................................................................................2
2.3. Pneumatic diagrams .......................................................................................................2
 SECTION 3

3. ELECTRIC AND ELECTRONIC PRINCIPLES ...............................................................................2

3.1. Introduction......................................................................................................................2
3.2. Electrical principles..........................................................................................................2
3.3. Power supply...................................................................................................................2
3.4. Electronic principles ........................................................................................................2
3.5. Interconnection diagrams................................................................................................2
 SECTION 4

4. MAINTENANCE PROCEDURES ...................................................................................................2

 SECTION 5

5. DATA OUTPUT FORMAT ..............................................................................................................2

 1 - INTRODUCTION

1. PRELIMINARIES .................................................................................................................................... 2
2. DESCRIPTION OF THE MANUAL.......................................................................................................... 2

RAA 006 A Ind.F Page 1/1 09.03.98

 2 - HYDRAULIC & PNEUMATIC PRINCIPLES

1. INTRODUCTION................................................................................................................................... 2
2. HYDRAULIC PARTS .............................................................................................................................. 2
3. PNEUMATIC DIAGRAMS..................................................................................................................... 2

RAA 006 A Ind.F Page 2/1 09.03.98

 3 - ELECTRIC & ELECTRONIC PRINCIPLES

1. INTRODUCTION................................................................................................................................... 2
2. ELECTRIC PRINCIPLES ......................................................................................................................... 2
3. POWER SUPPLY ................................................................................................................................... 2
4. ELECTRONIC PRINCIPLES.................................................................................................................... 2
5. INTERCONNECTION DIAGRAMS........................................................................................................ 2

RAA 006 A Ind.F Page 3/1 09.03.98

DIAGNOSTICS
DIAGNOSTICS
 4 - MICROS MAINTENANCE PROCEDURES

1. CUSTOMER MAINTENANCE ............................................................................................................... 2

1.1 Introduction ......................................................................................................................... 2
1.2. Daily maintenance............................................................................................................ 2
1.3. Weekly maintenance........................................................................................................ 2
2. SERVICE TECHNICIAN MAINTENANCE AND ADJUSTMENTS ............................................................ 2
2.1. Frequency chart table ...................................................................................................... 2
A - Hydraulic ................................................................................................................. 3
B - Mechanical ............................................................................................................. 3
D - Electric & Electronic............................................................................................... 4
2.2. MICROS maintenance kit ................................................................................................. 4
3. PROCEDURES ...................................................................................................................................... 4
4 - MAINTENANCE PROCEDURES

1. CUSTOMER MAINTENANCE

1.1 Introduction

WARNING ! : Customer maintenance has to be carried out according to the

recommended frequency chart table and after having performed an ABX
approved customer training course. The system warranty may be affected
if damage occurs after an untrained technician intervenes or if
replacement spare parts and consumables do not come from an ABX
approved origin.

1.2. Daily maintenance

No special adjustments or maintenance has to be done on your equipment
if the recommended start up and shut down procedures are explicitly
respected.
See the MICROS users' manual for the daily rinsing and cleaning of the
system.

1.3. Weekly maintenance

- General cleanliness
An overall check for cleanliness of the system is recommended every week.
All traces of blood or reagent have to be wiped off as soon as possible using
a piece of cloth and distilled water.

WARNING ! : Never use solvent or an abrasive cleaning material to clean the system.

2. SERVICE TECHNICIAN MAINTENANCE AND ADJUSTMENTS

2.1. Frequency chart table

The yearly maintenance frequencies vary with the instrument output.
According to the cycle numbers, 3 types are created :

- Type 1 : < 6000 cycles/year -> 2 overall maintenances/year

- Type 2 : 6000 to 15000 cycles/year -> 2 overall maintenances/year

- Type 3 : > 15000 cycles/year -> 3 overall maintenances/year

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RAA 006 A Ind.F 10/03/98
 4 - MAINTENANCE PROCEDURES

A - Hydraulic

Hydraulic assy. Workload Frequency

TYPE TYPE TYPE
1 2 3
RBC chamber - Chamber cleaning 2 2 3
- Aperture check 1 2 3
- Aperture O Ring 1 1 1
replacement 1 1 1
- Coaxial O ring
replacement
WBC/HGB chamber - Chamber cleaning 2 2 3
- Aperture check 1 2 3
- O Ring replacement 1 1 1
- Coaxial O ring 1 1 1
replacement
Mixing chamber - Cleaning 2 2 3
Vacuum/waste syringe - Cleaning 1 2 3
- O ring replacement 1 2 3
- watertightness check 2 2 3
- Piston replacement 0,5 1 1
Liquid syringe - Lyse O ring replacement 1 1 1
- Diluent O ring 1 1 1
replacement 1 1 1
- Syringe O ring
replacement
Sampling & piercing - O ring replacements 1 1 1
needle
HGB spectrophotometer - Cleaning 2 2 3
- HGB blank 2 2 3
check/adjustment
Reagent pack - Connector O ring replacts 1 1 1
Pressure - Check 2 2 3

B - Mechanical

Mechanical assy. Workload Frequency

TYPE TYPE TYPE
1 2 3
Needle carriage - Axis lubrication 2 2 3
- Right pulley operation 1 2 3
check 1 2 2
- Notched belt tightness 2 2 3
- Operation check
Needle - Axis lubrication 2 2 3
- Upper pulley operation 1 2 3
check 1 2 2
- Notched belt tightness 2 2 3
- Operation check
Piercing compartment - Cleaning 2 2 3
- Door operation check 2 2 3

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RAA 006 A Ind.F 10/03/98
4 - MAINTENANCE PROCEDURES

D - Electric & Electronic

Assy Workload Frequency

Mother board - RBC/PLT gain check/adjustment 1 1 1
- WBC gain check/adjustment 1 1 1
- Aperture voltage 1 2 3
check/adjustment 1 1 1
- Drain detection 1 1 1
check/adjustment 1 1 1
- Voltage supply 2 2 3
checks/adjustments
- Threshold checks/adjustments
- HGB blank check/adjustment
Temperature sensor - Temperature check/adjustment 1 2 3
Fan - Operation check 1 2 3
Chip card - Operation check 1 1 1

2.2. MICROS maintenance kit

* O ring kit : XEA 328 AS
This kit includes :

FAA 036 A 1 Lyse dispenser O ring

FAA 029 A 1 Diluent dispenser O ring
FAA 055 A 2 Sampling syringe O rings
FAA 049 A 2 Aperture O rings
FAA 046 A 2 Coaxial O rings
FAA 054 A 2 Sampling needle O rings (MICROS CT)
FAA 053 A 1 Sampling needle O ring (MICROS OT)
FAA 017 A 1 Vacuum/waste syringe O ring
KAM 022 A 6 Board holder clips.

3. PROCEDURES

NOTE : Maintenance and adjustments that need to be done on MICROS are

divided into "procedures"according to concerned assemblies. This should
make any updating easier as all interventions can be done with the
corresponding "procedure" on its own.

CONCERNED PARTS :
- Hydraulic maintenance and adjustments
- Pneumatic maintenance and adjustments
- Electrical maintenance and adjustments
- Power supply maintenance and adjustments
- Electronic maintenance and adjustments
- Printer maintenance and setup

CAUTION : Each procedure has to be read entirely before the intervention is begun.

WARNING ! : When cleaning instruments, disposable gloves should be worn.

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RAA 006 A Ind.F 10/03/98
 4 - MAINTENANCE PROCEDURES

NOTE : 1 - Unless precisely mentioned, each intervention on MICROS begins with

the cover dismantling :
Open first the front door to pneumatical part. Using a 2.5 screwdriver,
unloosen the locker screw as shown on the Diag.4.1 below. Unscrew the
cover fixation screws (Diag.4.2) and remove the cover.
2 - The instrument must be switched off for each intervention that does not
require screen display or voltage supplies.
3 - It is mandatory to reconnect the silicone sleeves on the valve nipples
that are indicated on the pneumatic diagram.
4 - It is possible to perform an "emergency stop" at any moment of a cycle
(except during the counting phasis) pressing the key until the "Bip"
is triggered. To restart the same cycle press once again the key.
5 - In a general way to move from menu to sub menu use the and
on the front panel to move the cursor in front of the selected
menu. Press the key to get in the menu and to exit. To
move directly into a menu it is possible to type in the menu number given
in the arborescence.

Fig.4.1

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RAA 006 A Ind.F 10/03/98
4 - MAINTENANCE PROCEDURES

Fig.4.2

P/N PROCEDURE CONCERNS

RAS 084 A DILUTION BLOCK MAINTENANCE - Lyse dispenser O ring replacement - Diluent
dispenser O ring replacement - Sampling
needle dispenser O ring replact.
RAS 085 A VAC/WASTE SYRINGE - O ring replacement
MAINTENANCE
RAS 086 A SAMPLING NEEDLE MAINTENANCE - Needle O ring replacement - Sampling
MICROS CT needle replacement - Piercing needle
replacement
RAS 087 A SAMPLING NEEDLE MAINTENANCE - Needle replacement - O ring replacement
MICROS OT
RAS 088 A CHAMBER MAINTENANCE - RBC, WBC/HGB, Mixing chamber cleaning -
Aperture O ring replacement - Coaxial O ring
replacement
RAS 089 A LIQUID VALVE MAINTENANCE - Liquid valve assy replacement - Valve
body replacement
RAS 090 A POWER SUPPLY - Voltage supply check - Power supply
CHECK/REPLACEMENT module replacement - Fan operation check.

Page 4/6
RAA 006 A Ind.F 10/03/98
 4 - MAINTENANCE PROCEDURES

RAS 091 A TECHNICIAN FUNCTIONS - HGB blank check/adjustment - Aperture

MICROS OT voltage check/adjustment - Pressure check -
WBC gain check/adjst - RBC/PLT gain
check/adjst - Temperature sensor adjustment
- RDW and PDW coeff. adjst - Cycle numbers
- Syringe positions for & extended shutdown -
Version and serial number display - Burning -
Needle & carriage sensor adjustment.
RAS 092 A MECHANIC FUNCTIONS - Sensor replacements. - Needle motion
check - Carriage motion check - Dilution
block motion check - Vac/Waste syringe
motion check - Valve operation check - LCD
contrast - Piercing mechanism check
RAS 093 A DRAIN DETECTION ADJUSTMENT - Drain detection sensor adjustment
RAS 094 A PCB VOLTAGE CHECKS - Voltage supply check/adj. - Aperture
voltage check/adj. - RBC threshold
check/adj. - WBC threshold check/adj. - PLT
threshold check/adj. - HGB blank voltage
check
RAS 121 A TECHNICIAN FUNCTIONS - HGB blank check/adjustment - Aperture
MICROS CT voltage check/adjustment - Pressure check -
WBC gain check/adjst. - RBC/PLT gain
check/adjst - Temperature sensor adjustment
- RDW and PDW coeff. adjst. - Cycle numbers
- Syringe positions for & extended shutdown -
Version and serial number display - Burning -
Needle position adjustment - Needle &
carriage sensor adjustment.
RAS 122 A CHANGING THE INSTRUMENT - Changing the instrument language
LANGUAGE
RAS 123 A REAGENT PACK - Connector O ring replacement
RAS 128 A MICROS TRANSFORMATION - Transformation of a MICROS "bottle" into a
MICROS "pack"
RAS 134 A AIR & LIQUID SYRINGES - Lubrification of the air and liquid syringes
RAS 138 A LX300 PRINTER - LX300 printer setup
RAS 161 A MICROS TRANSFORMATION - Transformation of a MICROS "pack" into a
MICROS "bottle"
RAS 163 A BARCODE READER Barcode reader setup

Page 4/7
RAA 006 A Ind.F 10/03/98
MICROS DILUTION BLOCK MAINTENANCE

* CONCERNS
- Lyse dispenser O ring replacement
- Diluent dispenser O ring replacement
- Sampling needle dispenser O ring replacement

* REQUIRED TOOLS
- Allen key : 2,5
2
- Dynamometric screw driver A302 : MAG 019 A
A301 : MAG 020 A

* REQUIRED PRODUCTS
- Silicone grease : LAM 004 A
- Soft paper

* INTERVENTION TIME
- 15min

* FREQUENCY
- On request or or on a leak case.
- O rings replacement : 1/year

* SPECIFIC KIT OR CONSUMABLES

- Micros O ring kit : XEA 328 AS

Page 1/5
RAS 084 A Ind.C 06.03.98
MICROS DILUTION BLOCK MAINTENANCE

* PROCEDURE

NOTE : In case of a leak on one of the three syringes it is recommended to

replace the entire dilution block. If not proceed as following :

- Pull the piston assy in the upper position and clamp the diluent and lyse
tubing as described on the diagram 1.
- Disconnect the diluent/lyse/sampling tubings from the 3 syringes and the
tube on the sampling syringe side.

Diag.1

- Unscrew the 4 fixation screws and remove the dilution block

(Diagram 1).

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RAS 084 A Ind.C 06.03.98
MICROS DILUTION BLOCK MAINTENANCE

Diag.2

- Unscrew the 6 screws and in order to remove the body cover

(Diagram 2).

- Pull out the pistons , and from the body with their respective o
ring still around (Diagram 3).

- Replace the lyse and diluent O rings by new ones. Check the cleanliness of
the piston and of the syringe bodies. If necessary clean with a soft paper.

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RAS 084 A Ind.C 06.03.98
MICROS DILUTION BLOCK MAINTENANCE

Diag.3

- Spread a little amount of silicone grease between two fingers and apply a
very thin film of grease on the 2 new sampling syringe O rings. Replace the
old ones .

- Reinstall the dilution block assy in the reverse order.

Use the A302 dynamometric screw driver to tighten the screws to
700mN/m (Diagram 2).
Use the A301 dynamometric screw driver to tighten the screws to
400mN/m (Diagram 2).
Apply the following torque to the screws (Diagram 1) : 700mN/m.

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RAS 084 A Ind.C 06.03.98
MICROS DILUTION BLOCK MAINTENANCE

* REMARKS

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RAS 084 A Ind.C 06.03.98
 MICROS VACUUM/WASTE SYRINGE MAINTENANCE

* CONCERNS
- O ring replacement.

* REQUIRED TOOLS
- Allen key : 2,5
- Dynamometric screw driver A302 : MAG 019 A

* REQUIRED PRODUCTS
- Silicone grease : LAM 004 A

* INTERVENTION TIME
- 10min

* FREQUENCY
- O ring replacement : - Type 1 : 1/year
- Type 2 : 2/year
- Type 3 : 3/year

* SPECIFIC KIT OR CONSUMABLES

- O ring kit : XEA 328 AS

Page 1/4
RAS 085 A Ind.C 10/03/98
 MICROS VACUUM/WASTE SYRINGE MAINTENANCE

* PROCEDURE
- Manually pull the syringe piston up in order to freed it from the syringe
body.

NOTE : The syringe has to be linked to the atmosphere, pressing the valve , to
pull the piston out from the syringe body.
Turn the cylindrical gearing by hand to help the raising of the piston
(See Diagram 1).

Diag.1

- Unscrew the fixation screws in order to remove the syringe body.

- Unscrew the O ring tightening screws and remove the O ring .

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RAS 085 A Ind.C 10/03/98
 MICROS VACUUM/WASTE SYRINGE MAINTENANCE

- Spread a little amount of silicone grease between two fingers and apply a
very thin film of grease on a new O ring.
- Reinstall in the reverse order. Apply the following torque to the screws :
700 mN/m.

CAUTION : Push the piston back inside the syringe body before tightening the o ring
fixation screws . Use the dynamometric screw driver to tighten the
screws to 400 mN/m

- Check the watertightness of the syringe running a "CHECK PRESSURE" cycle

(see procedure : RAS 091 A : Technician functions).

- Run cycles and check for correct operations.

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RAS 085 A Ind.C 10/03/98
 MICROS VACUUM/WASTE SYRINGE MAINTENANCE

* REMARKS

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RAS 085 A Ind.C 10/03/98
 MICROS CT SAMPLING NEEDLE MAINTENANCE

* CONCERNS
- Needle O ring replacement
- Sampling needle replacement
- Piercing needle replacement

* REQUIRED TOOLS
- Allen key : 2,5
- Dynamometric screw driver A302 : MAG 019 A

* REQUIRED PRODUCTS
- Silicone grease : LAM 004 A

* INTERVENTION TIME
- 15min

* FREQUENCY
- See frequency chart table for cleaning.
- O ring replacement : 1/year
- Needle replacement : On request only

* SPECIFIC KIT OR CONSUMABLES

- O ring kit : XEA 328 AS
- MICROS CT sampling needle : GBC 052 A
- Piercing needle : GBC 122 A

Page 1/5
RAS 086 A Ind.C 09/03/98
 MICROS CT SAMPLING NEEDLE MAINTENANCE

* PROCEDURE
A - Needle or O ring replacement
- Disconnect the tube from the top of the needle . Manually lift up
the sampling needle (Diagram 1).
- Unscrew the 2 screws in order to freed the needle rinsing block
from the carriage frame .

Diag.1

- Remove the clip and then the rinsing block/needle assy from the
carriage taking care not to bend the needle.

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RAS 086 A Ind.C 09/03/98
 MICROS CT SAMPLING NEEDLE MAINTENANCE

- Lift up the O ring holder and replace the O rings by new ones
previously greased. Wipe all excess of grease away.
- If necessary clean the inner surface of the rinsing block with a little piece of
paper.

Diag.2

NOTE : Proceed the same way to replace the needle if necessary

- Reassemble in the reverse order. Use a dynamometric screw driver to

tighten the screws (Diag.1) to 700 mN/m.

B - Piercing needle replacement

- Disconnect the tube from the piercing needle (Diag.3).

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RAS 086 A Ind.C 09/03/98
 MICROS CT SAMPLING NEEDLE MAINTENANCE

Diag.3
- Lift the needle in the upper position.

- Loosen the 2 screws just enough to enable the rinsing block to be lifted
up of about 5 mm.
- Pull the piercing needle foreward and replace it by a new one.
- Reassemble in the reverse order. Apply the same torque : 700mN/m to
tighten the two screws .

NOTE : It is recommended to check the correct motion of the needle. Proceed as

following :
Enter the "SERVICE" menu and then the sub menu "MECHANIC" and
perform a "NEEDLE U/D" cycle.

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RAS 086 A Ind.C 09/03/98
 MICROS CT SAMPLING NEEDLE MAINTENANCE

* REMARKS
Blockage problems may occur on some instruments during the needle or
carriage motions giving some motor error messages.

Before replacing the concerned motor, it is necessary to check the correct

rotation of the free puley located at the end of the notched belt.

Remove the axle screw of the puley and clean its 2 parts and the
washer.Reinstall the puley assy, the rounded edge facing the puley. Tighten
the screw with a torque of 400mN/m.

Check that the puley turns freely after the tightening. Add a drop of oil (LAM
007 A) if necessary.

A service kit (XEA 343 AS) will be available from May 1995 and will include :

1 - FAG 011 A : Autolub. axle

2 - GBC 146 A : Free pulley
3 - GBC 147 A : Pulley holder

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RAS 086 A Ind.C 09/03/98
 MICROS OT SAMPLING NEEDLE MAINTENANCE

* CONCERNS
- Needle replacement
- O ring replacement

* REQUIRED TOOLS
- Allen key : 2,5
- Dynamometric screw driver A300 : MAG 013 A

* REQUIRED PRODUCTS
- Silicone grease : LAM 004 A

* INTERVENTION TIME
- 15 min

* FREQUENCY
- Needle replacement : On request only
- O ring replacements : 1/year

* SPECIFIC KIT OR CONSUMABLES

- O ring kit : XEA 328 AS

Page 1/3
RAS 087 A Ind.C 09/03/98
 MICROS OT SAMPLING NEEDLE MAINTENANCE

* PROCEDURE
- Disconnect the tube from the top of the needle . Manually lift up
the sampling needle (Diagram 1).
- Unscrew the 2 screws in order to freed the needle rinsing block
from the carriage frame. Remove the clip .

Diag.1

- Remove the rinsing block/needle assy from the carriage taking care not to
bend the needle.

- If necessary clean the inner surface of the rinsing block by means of a little
piece of soft paper. Spread a little amount of grease in between the rinsing
block and its support.
- Lift up the O ring holder and replace the O ring by a new one
previously greased. Wipe all excess of grease away.

NOTE : Proceed the same way to replace the needle if necessary

- Reassemble in the reverse order. Use a dynamometric screw driver to

tighten the screws (Diag.1) to 100 mN/m.

NOTE : It is recommended to check the correct motion of the needle. Proceed as

following :
Enter the "service" menu and then the sub menu "Mechanic" and perform
a "needle up/down" cycle.

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RAS 087 A Ind.C 09/03/98
 MICROS OT SAMPLING NEEDLE MAINTENANCE

* REMARKS
Blockage problems may occur on some instruments during the needle or
carriage motions giving some motor error messages.

Before replacing the concerned motor, it is necessary to check the correct

rotation of the free puley located at the end of the notched belt.

Remove the axle screw of the puley and clean its 2 parts and the
washer.Reinstall the puley assy, the rounded edge facing the puley. Tighten
the screw with a torque of 400mN/m.

Check that the puley turns freely after the tightening. Add a drop of oil (LAM
007 A) if necessary.

A service kit (XEA 343 AS) will be available from May 1995 and will include :

1 - FAG 011 A : Autolub. axle

2 - GBC 146 A : Free pulley
3 - GBC 147 A : Pulley holder

Page 3/3
RAS 087 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

* CONCERNS
- RBC, WBC/HGB, Mixing chamber cleaning
- Aperture O ring replacement
- Coaxial O ring replacement

* REQUIRED TOOLS
- Allen key : 2,5
- Soft paper
- Dynamometric screw driver : MAG 013 A
- Cutting pliers

* REQUIRED PRODUCTS
- Liquid soap
- Distilled water
- A scalpel
- A Micropipette tip

* INTERVENTION TIME
- 30 min

* FREQUENCY
- RBC, WBC, Mixing chamber cleaning : 2 (type 1 & 2) or 3/year (type 3).
- Aperture O ring replacement : 1/year
- Electrode O ring replacement : 1/year

* SPECIFIC KIT OR CONSUMABLES

- O ring kit : XEA 328 AS

Page 1/7
RAS 088 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

* PROCEDURE
1 - RBC chamber cleaning :

- Run a drain chamber cycle ("SERVICE" menu, "DRAIN CHAMBERS " Sub
menu).
- Record the tube positions before dismantling the chambers.
- Disconnect the chamber tubes.
- Unclip the RBC chamber. Dismantle the electrode loosening the 2 fixation
screws (Diagram 1).

Diag.1

- Use a previously cut micropipette tip to change the electrode O ring as

shown below (Diag.2).

Page 2/7
RAS 088 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

- Clean the groove and replace the O ring , using the clamp as shown on
Diag.2.

Diag.2

- Dismantle the counting head as described below :

- Install the chamber over a piece of white paper or cloth.

Page 3/7
RAS 088 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

- Remove the fixation screws from the counting head (See diag.3).

Diag.3

- Carefully remove the counting head and plunge the aperture in

distilled water.

WARNING ! : Do not manipulate the aperture using hard instruments. Clean the aperture
with a piece of soft paper or preferably, in between 2 fingers.

- Clean the chamber and the counting head with liquid soap, do not
introduce any sharp instruments inside so as to avoid damaging the inside
of the chamber and the aperture.
- Rinse thoroughly with distilled water
- Dry the exterior of the chamber with a soft paper.
- Re-assemble the chamber. Replace the O ring (Diag.3).

WARNING ! : Do not apply too much pressure on the counting head or on the electrode
fixation screws, as it can break the aperture (tightening torque =
100mN/m).

- Position the chamber in its fixation clips.

- Reconnect the tubes

Page 4/7
RAS 088 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

2 - WBC chamber
- Run a drain chamber cycle ("SERVICE" menu, "DRAIN CHAMBERS" Sub
menu).
- Loosen the cover screws of the WBC chamber and remove the cover.
- Record the tube positions before dismantling the chamber.
- Disconnect the chamber tubes and top.
- Unclip the WBC chamber. Unscrew the photometer fixation screws
(Diagram 4).
Remove, clean the WBC chamber and replace the O rings as described in
"1 - RBC chamber".

Diag.4

- Check the cleanliness of the photometer (inner side). If necessary clean

the surfaces with a soft paper. Make sure that the bottom surfaces of the
WBC chamber is clean too.
Reinstall the photometer on the chamber. Use a dynamometric screw driver
and adjust the torque to 400mN/m when tightening the 2 screws .

CAUTION : After having operated on the photometer it is necessary to readjust the

Hgb blank as described on the procedure : RAS 091A.

Page 5/7
RAS 088 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

3 - Mixing chamber
- Run a drain chamber cycle ("Service" menu, "Drain chambers" Sub menu).
- Record the tube positions before dismantling the chamber.
- Unclip the chamber and clean it with liquid soap.
- Rinse thoroughly with distilled water
- Dry the exterior of the chamber with a soft paper.
- Re install in the reverse order.

Page 6/7
RAS 088 A Ind.C 09/03/98
 MICROS CHAMBER MAINTENANCE

* REMARKS
- Once the cleaning done on the chambers, start the instrument and check
for leaks.
- Check the calibration too.

Page 7/7
RAS 088 A Ind.C 09/03/98
 MICROS LIQUID VALVE MAINTENANCE

* CONCERNS
- Liquid valve assy replacement
- Valve body replacement

* REQUIRED TOOLS
- Allen key
- Flat screw driver

* REQUIRED PRODUCTS
- Soft paper

* INTERVENTION TIME
- 15 min

* FREQUENCY
- On request only

* SPECIFIC KIT OR CONSUMABLES

- 6 Valve assembly (MICROS CT) : XDA 478 B
- 5 Valve assembly (MICROS OT) : XDA 479 B
- 7 Valve assembly : XDA 477 B
- 12 Valve assembly : XDA 520 B (MICROS OT)
- 13 Valve assembly : XDA 521 B (MICROS CT)
- 2 ways NC liquid valve without solenoïd : XDA 481 B
- 3 ways liquid valve without solenoïd : XDA 483 B
- Solenoïd 24V 4W : EAZ 004 A

Page 1/4
RAS 089 A Ind.C 09/03/98
 MICROS LIQUID VALVE MAINTENANCE

* PROCEDURE
- Several reasons can unabled the correct operations of the valves :

- Leaks on the valve bodies

- Defective Solenoïds
- Liquid discharge on valves
- corrosion traces on the axis, ect...

- Run a "drain chambers" cycle.

- Switch off the instrument.

- Note the tube positions on the valve assembly that requires to be

dismantled.

- Unscrew the fixation screws (Diag.1, 2 or 3) and disconnect the

connector .

Diag.1

Page 2/4
RAS 089 A Ind.C 09/03/98
 MICROS LIQUID VALVE MAINTENANCE

Diag.2 & 3

NOTE : When leaks occur on valves it is recommended to replace the entire valve
assembly by a new one.

- If only one valve has been damaged it is possible to dismantle the valve
body on its own as shown on the diagram 4 : use a screw driver to
disconnect the valve holder clip and remove the body.

Diag.4

Page 3/4
RAS 089 A Ind.C 09/03/98
 MICROS LIQUID VALVE MAINTENANCE

WARNING ! : When replacing one valve only, it is important to check if the

"neighboured" valves have not been damaged too.

CAUTION : The solenoïd can not be dismantled unless unsoldering it.

If this one is suspected solder a new one or replace the entire valve
assembly.

- Reconnect the tubes on the nipples (with the sleeves).

- Re-install in the reverse order. Switch on the instrument.
- Control the watertightness of the valves and check for the correct
operations :
Go to "SERVICE" menu, then to "MECHANIC" sub menu (6) and require a
"VALVES" (6) test (see Mechanic functions : RAS 092 A).
- Check the calibration too.

* REMARKS
When piercing several times the same tube cap, some pieces of cork may
be dragged along towards the mixing chamber, the WBC chamber and
then the liquid valve .
This may damaged the operation of the valve.

Page 4/4
RAS 089 A Ind.C 09/03/98
 MICROS POWER SUPPLY CHECK AND REPLACEMENT

* CONCERNS
- Voltage supply check
- Power supply module replacement
- Fan operation check

* REQUIRED TOOLS
- Allen key : 2,5
2
- Flat screw driver
- Volmeter

* REQUIRED PRODUCTS
- None

* INTERVENTION TIME
- 15 min

* FREQUENCY
- 1/year

* SPECIFIC KIT OR CONSUMABLES

- None

Page 1/5
RAS 090 A Ind.C 09/03/98
 MICROS POWER SUPPLY CHECK AND REPLACEMENT

* PROCEDURE
1 - Supply voltage check

CAUTION : The supply voltage check has to be done with the power supply module
connected only.

- The supply voltage check is done on the mother board (XAA 355 A)
according to the following chart table :

* Ground on TP31, TP 30 or TP 29

TEST POINTS VOLTAGES

TP 20 -12V +0,5V
TP 22 24V +0,6V -0V
TP 23 5V +0,3V -0V
TP21 12V + 0,5V

WARNING ! : The two screws on the top of the coaxial cover holding the board on the
MICROS frame are the grounding connection of the mother board.
It is mandatory to chek the correct tightening of these screws to obtain
correct voltage values.

Page 2/5
RAS 090 A Ind.C 09/03/98
 MICROS POWER SUPPLY CHECK AND REPLACEMENT

The test points are located as shown on the diagram below :

MICROS MOTHER BOARD

J38 J39 TP31 TP30 TP29

GND GND GND

RBC COAX WBC COAX

SUPPLIES
-12V +- 0.5
+0.6 TP20
24V -0 TP22
5V +0.3
-0 TP23
12V -+0.5TP21

SUPPLY

+5V

GND

J26
+24V

+12V
-12V

Diag.1

NOTE : If the voltages values are not correct or among the ranges no adjustment
can be carried out either on the board or on the power supply module.
Replace the power supply module as described below :

Page 3/5
RAS 090 A Ind.C 09/03/98
 MICROS POWER SUPPLY CHECK AND REPLACEMENT

2 - Power supply module replacement

- Switch off the instrument.
- Disconnect the main supply voltage cable and the printer cable.
- Disconnect the power supply cable from the mother board, connector J26
(see Diag.1).
- Unscrew the 4 screws (Diag.2) and start to move out the power supply
module. Disconnect the grounding wire (Yellow/green wire) from the rear
panel of the module. Route the cable (from J26) down to make the
removing of the module easier.

Diag.2

WARNING ! : The power supply module internal fuse is not to be replaced even when
this one has blown down.

- Replace the power supply module by a new one and reinstall in the
reverse order.
- Switch on the instrument and check the voltages on the mother board as
described in the previous paragraph.

Page 4/5
RAS 090 A Ind.C 09/03/98
 MICROS POWER SUPPLY CHECK AND REPLACEMENT

* REMARKS
The four leds in front of the test points are lit to indicate a voltage presence
but whatever its value!!!

Check the operation of the fan as following : When the fan has stopped,
move the cursor to the 4 SERVICE menu and press ENTER : the fan should
start.

Page 5/5
RAS 090 A Ind.C 09/03/98
 MICROS OT TECHNICIAN FUNCTIONS

* CONCERNS

- HGB photometer calibration

- Aperture voltage check/adjustment
- Pressure check
- WBC gain check/adjustment
- RBC, PLT gain checks/adjustments
- Temperature sensor adjustment
- Cycle numbers
- Version and serial number display.
- Burning
- Sensor adjustment
- Vacuum adjustment/check

* REQUIRED TOOLS
- Flat screw driver
- thermometer
- Barflex
- Allen key : 2,5
- Voltmeter

* REQUIRED PRODUCTS
- WBC latex : LAD 001 AS
- RBC and PLT latex : LAD 002 AS
- Soft paper
- Flat piece of stiff plastic

* INTERVENTION TIME
- 60 min

* FREQUENCY
- See maintenance chart table.

* SPECIFIC KIT OR CONSUMABLES

- None

RAS 091 A Ind.D Page 1/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

* PROCEDURE
The arborescence of the «technician functions» is the following :
1 - CAL. PHOTOMETER 1 - WBC ADJUST
1 - VERSION

2 - APERTURE VOLTAGE 2 - # LYM < >

3 - CHECK PRESSURE 3 - # GRA < >

4 - WBC ADJUST 1 - RBC PLT ADJUST

2 - ADJUSTMENTS
2 - MCV < >

3 - MPV < >

5 - RBC PLT ADJUST
1 - NEEDLE SENSOR

2 - NEEDLE < >

6 - SENSOR ADJUST
3 - TRANSFER SENSOR

7 - VACUUM
1 - VACUUM < >

2 - CHECK
8 - TECHNICIAN
1 - UNCAL. TEMPERATURE

3 - TEMPERATURE 2 - CALIBRATE
1 - VISIBLE
3 - TEMPERATURE
2 - INVISIBLE
MICROS OT
1 - CONTROL
4 - RUN MODE
2 - USER

1 - YES
5 - REAGENT PACK
2 - NO

6 - SERIAL NUMBER

1 - STARTUP # < >

7 - CYCLE NUMBER 2 - STANDBY # < >

3 - CBC # < >

1 - BURNING #

8 - BURNING 2 - BURNING

3 - CONTROL

RAS 091 A Ind.D Page 2/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

Once entering the «SERVICE» sub menu, move to «TECHNICIAN FUNCTIONS» and press .
A specific password (421) is required to enter the sub menus.
Move the cursor by means of and and choose the required menus pressing the
key.

I - The version number is displayed.

II - Adjustments :

1 - HGB photometer calibration

- Dismantle the WBC chamber cover.

- Check that the WBC chamber top is well positioned.

- Check the general cleanliness of the WBC chamber/spectrophotometer assy.

- Re-install the chamber cover.

NOTE - If the WBC chamber has been dismantled previously make sure no liquid has flown
in between the spectrophotomer and the chamber.
Clean the inner surfaces of the spectrophotometer as well as the chamber.
Reassemble the assy and tighten the two screws to the following torque : 400mN/m
(see RAS 088 A : Chamber maintenance)

- Run the CALIBRATION PHOTOMETER function (function 1 of the «ADJUSTEMENTS» menu) :

diluent is delivered to the mixing chamber and transferred to the WBC chamber twice.

An HGB channel is displayed on the LCD screen representing a voltage value as shown below :

VALUE
237

The HGB photometer calibration must be done 20min at least after the
IMPORTANT instrument has been switched on.

- By means of R97 (See diagram 1) adjust the HGB channel according to the room temperature using
the chart table given on next page.

IMPORTANT This adjustment must be done with the WBC chamber cover installed!!!

- Press a key to escape when the adjustment is done.

NOTE After 40 seconds approwimately, the function is automatically exited.

RAS 091 A Ind.D Page 3/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

MICROS MOTHER BOARD

J38 J39 TP31 TP30 TP29

GND GND GND
J21

RBC COAX WBC COAX

HGB
R97 TP27

TP19

APERTURE R91
VOLTAGE
60V +0.1
-

Diag.1

ROOM CHANNEL VOLTAGE

TPT (°C) Mini. Nominal Maxi. Mini. Nominal Maxi
15 240 245 250 4.71 4.81 4.91
16 240 245 250 4.70 4.79 4.89
17 239 244 249 4.68 4.78 4.88
18 238 243 248 4.66 4.76 4.86
19 237 242 247 4.65 4.74 4.84
20 236 241 246 4.63 4.73 4.83
21 235 240 245 4.61 4.71 4.81
22 234 239 244 4.60 4.69 4.79
23 234 239 244 4.58 4.68 4.77
24 233 238 243 4.56 4.66 4.76
25 232 237 242 4.55 4.64 4.74
26 231 236 241 4.53 4.63 4.72
27 230 235 240 4.51 4.61 4.71
28 229 234 239 4.49 4.59 4.69
29 228 233 238 4.48 4.58 4.67
30 228 233 238 4.46 4.56 4.66
31 227 232 237 4.44 4.54 4.64
32 226 231 236 4.43 4.53 4.62
33 225 230 235 4.41 4.51 4.61
34 224 229 234 4.39 4.49 4.59
Table 1 35 223 228 233 4.38 4.47 4.57

RAS 091 A Ind.D Page 4/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

2 - Aperture voltage

- Once entering the «APERTURE VOLTAGE» menu, connect a voltmeter between the ground (TP30)
and TP19.

- Adjust to 60V + 0,1V by means of R91 (Diagram 1).

The aperture voltage adjustment is only available on the «aperture vol-

IMPORTANT tage» menu. However it is possible to check it during the counting.

- Press any key to escape.

3 - Pressure check

- Disconnect the tube from the vacuum/waste syringe

coming from the valve (see diagram 2).

- Follow the instructions given on the LCD screen :

«PLEASE PLUG BARFLEX ON AIR SYRINGE» (On

the free nipple).

«CHECK PRESSURE : -200mB + 10mB» (The piston

has raised in order to create a vacuum in the syringe
body).

«PLEASE RECONNECT PREVIOUS TUBE»

(disconnect the Barflex and replug the tube instead).

- Check the stability of the vacuum during 30 secon-

des : The vacuum drop down must be < 2 mbar.

- If the results are not correct check the O ring and the
Diag.2 tubing watertightness.

4 - WBC adjust

- Put the WBC latex to mix on a Vortex during 5min or shake thoroughly

As the WBC gain is a factory adjustment it is mandatory not to readjust it

IMPORTANT without taking the following precautions :
Carry out previously an autoconcentrated cleaning to make sure of the
cleanliness of the WBC counting circuit.
If necessary clean the WBC chamber aperture as described in RAS 088 A.
Make sure the Latex has been thoroughly mixed before.

- Run a blank cycle to check the cleanliness of the instrument.

- Enter the «WBC ADJUST» sub menu.

RAS 091 A Ind.D Page 5/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

- Present the vial of Latex to the open probe as shown

on diagram 3 and press the sampling bar located behind
the sampling needle : an analysis cycle begins.

During the cycle measuring phasis (around 1 minute)

the Lymphocyte and the Granulocyte volumes are
displayed on the screen every 3 seconds as shown
below :
Diag.3

LYM <57 +/- 1> GRA <180 +/- 2>

57 180

- Wait for several results to be displayed and check the stability of both values.

It is mandatory not to operate the gain adjustment as long as the lympho

IMPORTANT and granulo values are not stable.

After one minute the last volume values displayed on the screen are printed out as well as the WBC,
PLT and RBC histograms and the CBC “s results. Check that these printed values correspond to the
following target values :
- LYM = 57 + 1
- GRA = 180 + 2

- If not rerun a «WBC ADJUST» cycle after having previously mixed the latex vial again. By means
of R74 (see diagram 4) adjust the volumes to the target values during the measuring phasis.

MICROS MOTHER BOARD

J38 J39 TP31 TP30 TP29

GND GND GND J21
J11 RBC COAX WBC COAX
Tpt SENSOR

PLT PULSE PROCESS HGB

THRESHOLD TP14 TP25 R97 TP27
TP18 TP26 180mV +3
- TP9 TP24 TP10
R16 R68
R89 R80
R86 R82 TP16 R73 TP19

THRESHOLD
THRESHOLD R75 350mV+5 APERTURE R91
400mV+5 TP13 - VOLTAGE
TP4 - R74 TP7
GAIN
R87 TP15
R81 GAIN 60V +0.1
-
J17 GAIN
TP11

GND TP29
NU
RBC PULSE PROCESS WBC PULSE PROCESS

Diag.4

RAS 091 A Ind.D Page 6/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

5 - RBC PLT adjust

- Put the RBC and PLT latex to mix on a Vortex during 5min or shake thoroughly

As the RBC/PLT gain is a factory adjustment it is mandatory not to readjust

IMPORTANT it without taking the following precautions :
Carry out previously an autoconcentrated cleaning to make sure of the
cleanliness of the RBC/PLT counting circuit.
If necessary clean the RBC/PLT chamber aperture as described in the
procedure RAS 088 A.
Make sure the Latex has been thoroughly mixed before.

- Run a blank cycle to check the cleanliness of the instrument.

- Enter the «RBC PLT ADJUST» sub menu.

- Present the vial of Latex to the open probe as shown on diagram 3 and press the sampling bar
located behind the sampling needle : the needle directly delivers the latex sample in the RBC chamber
dilution (for a usual analysis cycle the sample is first delivers to the mixing chamber) and a measuring
phasis begins.

- During the cycle measuring phasis (around 1 minute) the Platelet and the Red Blood cell volumes
are displayed on the screen every 3 seconds as shown below :

RBC <74 +/- 1> PLT <59 +/-1>

74 59

- Wait for several results to be displayed and check the stability of both values.

It is mandatory not to operate the gain adjustment as long as the platelet

IMPORTANT and RBC values are not stable.

After one minute the last volume values displayed on the screen are printed out as well as the PLT
and RBC histograms and the CBC “s results.

- Check that these printed values correspond to the following target values :

- RBC = 74 + 1
- PLT = 59 + 1
- If not rerun a «RBC PLT ADJUST» cycle after having previously mixed the latex vial again.

- Adjust the volumes to the target values during the measuring phasis by means of the following
potentiometers (see Diagram 4) :

- RBC gain with R81

- PLT gain with R87

RAS 091 A Ind.D Page 7/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

6 - Adjust sensors

* Needle sensor

If the needle detector 1 (see diagram 5) has been

replaced by a new one or dismantled for any reason, it
is mandatory to re-position it at the right heigth. Proceed
as following :

- Install the piece of plastic 1 (diagram 6) underneath

the needle rinsing block 2 .

Diag.5

- Once entering the menu «ADJUST SENSORS»

(Selection 6), enter the sub menu «NEEDLE
SENSOR».
- Push the sampling needle 3 downward until it stops
against the piece of plastic and press any key in order
to raise the needle back in its upper position.
The current number of steps, the mini and maxi values
are displayed as well as the way to remove the sensor
(shown by an arrow) if the current value is out of ran-
ges (see below).

Diag.6

CURRENT : 62 MIN : 65 MAX : 75

- For a current number of steps out of ranges, unloosen the 2 screws 2 (diagram 5) and gently move
the sensor - upward if the current value is too low
- downward if the current value is too high.

NOTE 10 steps correspond to around 1 mm. The target number of steps is 70 + 5

- Tighten the screws 2 and rerun a «NEEDLE SENSOR» cycle. Check that the current value is
correct.

RAS 091 A Ind.D Page 8/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

* Needle < >

This adjustment is intended to a factory use. It is mandatory not to readjust

IMPORTANT it without taking the following precautions :
Carry out previously an autoconcentrated cleaning to make sure of the
cleanliness of the RBC/PLT counting circuit.
If necessary clean the RBC/PLT chamber aperture as described in the
procedure RAS 088 A.

This function allows the adjustment of the needle heigth in the RBC chamber. The current number of
steps is 630 and may be adjusted if reproducibilty on RBC/PLT is incorrect.

In this case the value has to be raised 10 steps by 10 steps. Reproducibilty on PLT has to be controled
between each value modification.

Normal ranges : Mini value : 580

Maxi value : 670
Proceed as follow :

- From the SENSOR ADJUST menu, move the cursor to the function <1> "NEEDLE SENSOR" and
press ENTER.

- Push the sampling carriage above the RBC/PLT chamber. Lower the sampling needle until it comes
into contact with the chamber edge as shown on the Diag.7.

Diag.7

- Press ENTER and record the number of step displayed on the LCD. Add 284 to the number displayed and
record the calculated value.

- Move the cursor to the function <2> "NEEDLE" and press ENTER. Enter the calculated value and validate.

RAS 091 A Ind.D Page 9/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

* Transfer sensor

This function allows the adjustment of the carriage

sensor 1 (diagram 7) position.

- Proceed as described below :

- Make sure the mixing chamber has been drained

previously and unclip it.

- Once entering the menu «ADJUST SENSORS»

(Selection 6), enter the sub menu «TRANSFER
SENSOR».
- Position the allen key 1 as to unloose the clip
tightening screw 2 (see Diagram 8).

- Hold the key as perpendicular as possible to the

chamber holder plate 4

Diag.8

- Move the carriage in front of the allen key and push

down the needle 3 until it stops against the allen key.
Adjust the position of the needle as central as possible
on the key.

- Press any key : the carriage comes back in its initial

position.

- The current number of steps, the mini and maxi va-

lues are displayed as well as the way to remove the
sensor if the current value is out of ranges (see below).
Diag.9

CURRENT : 524 MIN : 528 MAX : 538 >

- For a current number of steps out of ranges, unloosen the 2 screws 2 (see diagram 8) and gently
move the sensor

- towards the right if the current value is too low

- towards the left if the current value is too high.

NOTE 10 steps correspond to around 1 mm. The target number of steps is 533 + 5

- Tighten the screws 2 (see diagram 8) and rerun a «CARRIAGE» cycle. Check that the current value
is correct.

RAS 091 A Ind.D Page 10/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

7 - Vacuum

This adjustement concerns the transfer of the mixing

chamber dilution towards the WBC chamber in order
to insure the HGB stability.

From the menu «ADJUSTMENTS», move to «7 - VA-

CUUM» and press ENTER.
Move to «2 - CHECK» and press ENTER. Liquids are
sent into the mixing chamber and then a chamber drain
begins.

Wait for the «beep» to be triggered off and check to

have liquids remaining in the chamber bottom nipple
(as shown on diag.10) when the «CHECK» cycle is
completed.
If liquids have not been drained correctly, it is possible
to adjust the vacuum value :
Diag.10

Move to «1 - VACUUM < >» and press ENTER. Raise the value if the drain has to be faster. Lower
the value if the drain has to be slower. Press ENTER to confirm. Rerun a «CHECK» cycle to control
the drain of the chamber.
Compare to this «CHECK» cycle , the chamber is totally drained during an analysis
NOTE
cycle.

Normal ranges : - Mini value : 140

- Maxi value : 200
- Default value : 170

III - Temperature adjustment

When entering the «temperature adjustment» menu the following sub menus are displayed :

1 - Uncal. temperature
When pressing the key the sensor temperature value uncalibrated is displayed. This value
should be equivalent to the diluent temperature + 2°.

2 - Calibrate :
The temperature must be calibrated according to the diluent temperature :

- Plunge a thermometer directly into the diluent container and leave it for a while until stabilization.

- Run 2 diluent primes («SERVICE» menu, «PRIME» sub menu, «DILUENT» selection).

- Enter the «CALIBRATE» menu. Note the temperature of the diluent and type in the value (if it is
different from the previous on the instrument).

- Press to save the new value.

RAS 091 A Ind.D Page 11/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

3 - Temperature :
1 - Visible : Press to validate the temperature display.
The value displayed is the calibrated temperature.
2 - Invisible : Press to cancel the temperature display.
(Temperature invisible by default)

IV - Run mode

The instrument must be configurated in the «USER» mode (configurated by default in the «USER «
mode), the «CONTROL» mode intends for a factory use.

V - Reagent pack

This function is used to update the instrument from a bottle mode to a pack mode.

VI - Serial #

Displays the instrument serial number.

VII - Cycle #

Displays the : Startup number since the first use of the instrument.
Stand by number since the first use of the instrument.
CBC number since the first use of the instrument.

The startup, stand by and CBC numbers are adjustable in this menu but
IMPORTANT it is mandatory to keep the initial values (useful for maintenance
schedules).

VIII - Burning

CAUTION : This function which allows the burning of the instrument is intended to a factory
use.

RAS 091 A Ind.D Page 12/13 08/08/96

 MICROS OT TECHNICIAN FUNCTIONS

*REMARKS

RAS 091 A Ind.D Page 13/13 08/08/96

 MICROS MECHANIC FUNCTIONS

* CONCERNS
- Sensor replacements
- Needle motion check
- Carriage motion check
- dilution block motion check
- Vacuum/waste syringe motion check
- Valve operation check
- LCD contrast adjustment
- Piercing mechanism check (MICROS CT)

* REQUIRED TOOLS
- Allen key : 2,5
- Felt-pen

* REQUIRED PRODUCTS
- None

* INTERVENTION TIME
- 30 min

* FREQUENCY
- See maintenance chart table.

* SPECIFIC KIT OR CONSUMABLES

- Vacuum/waste syringe sensor : XBA 228 AS
- Liquid syringe sensor : XBA 228 AS
- Carriage and needle sensors : XBA 250 A

Page 1/7
RAS 092 A Ind.C 10/03/98
 MICROS MECHANIC FUNCTIONS

* PROCEDURE
Enter the "4 - SERVICE" menu and move to "6 - MECHANIC" sub menu.
The following operations are available :

1 - Check sensors : Press ;

"NEEDLE SENSOR 0
CARRIAGE SENSOR 0"
should be displayed on the LCD screen

Diag.1

Page 2/7
RAS 092 A Ind.C 10/03/98
 MICROS MECHANIC FUNCTIONS

- Manually raise the needle support in the upper position. The "0" should
switch to "1". This indicates the correct operation of the needle sensor
(diagram 1).
If nothing happened try to move the needle up and down again to get the
commutation from "0" to "1".
If the test is still wrong, check the correct connection of the sensor on the
connector J7 of the mother board.

* Replacing the needle sensor

Switch off the instrument.
Use a felt-pen to mark the sensor position.
Unscrew the 2 screws and remove the cell holder .
Disconnect the wire from the J7 connector (See diagram 3).
Replace the previous sensor by a new one and reassemble in the reverse
order.

WARNING ! : The new sensor must be installed exactly on the same position.

Proceed as described in RAS 091 A (MICROS OT) or RAS 121 A (MICROS CT)
"Sensor adjustment" to check the correct position of the sensor.

* Replacing the carriage sensor

Use the "CHECK SENSORS" test to check the carriage motion detection :
Move rightward the needle carriage in order to perform the sensor
detection (diagram 2).

Diag.2

Page 3/7
RAS 092 A Ind.C 10/03/98
 MICROS MECHANIC FUNCTIONS

If needed to replace, note its position with a felt-pen, unscrew the 2 screws
and remove the cell holder .
Disconnect the wire from the connector J8 (See diagram 3)
Replace the previous sensor by a new one and reassemble in the reverse
order.

WARNING ! : The new sensor must be installed exactly on the same position.

Proceed as described in RAS 091 A (MICROS OT) or RAS 121 A (MICROS CT)
"Sensor adjustment" to check the correct position of the sensor.

Diag.3

2 - Dilution block motion check

- Run a "4 - LIQUID SYRINGE" cycle : The syringes are moved upward and
downward once.
If the sensor detection is not correct a message "ERROR : SYRINGE
MOTOR" is displayed.

Three parts are involved :

- The motor ------> Check the connection on the board (connector J3)
- The syringes ------> Check the motion up/down
- The sensor ------> Check the connection on the board or replace it.

Page 4/7
RAS 092 A Ind.C 10/03/98
 MICROS MECHANIC FUNCTIONS

Diag.4

* Replacing the sensor :

- Switch off the instrument.

- Unscrew the 2 screws (Diag.4) and disconnect the wire from the
connector J9 (Diag.3)
- Replace the previous sensor by a new one and re-install in the reverse
order.
- Switch on the instrument.
- Run a "4 - LIQUID SYRINGE" cycle again to control the correct detection.

3 - Vacuum/waste syringe motion check

- Run a "5 - PRESSURE SYRINGE" cycle : The syringe piston is moved
upward and downward once (Diag.5).
If the sensor detection is not correct a message "ERROR : PRESSURE
SYRINGE MOTOR" is displayed.

Page 5/7
RAS 092 A Ind.C 10/03/98
 MICROS MECHANIC FUNCTIONS

Three parts are involved :

- The motor ------> Check the connection on the board (connector J4)
- The syringe ------> Check the motion up/down
- The sensor ------> Check the connection on the board or replace it.

Diag.5

* Replacing the sensor :

- Switch off the instrument.

- Unscrew the 2 screws Erreur! Nom de fichier incorrect. (Diag.5) and
disconnect the wire from the connector J10 (Diag.3)
- Replace the previous sensor by a new one and re-install in the reverse
order.
- Switch on the instrument.
- Run a "5 - PRESSURE SYRINGE" cycle again to control the correct detection.

4 - Valve operation check

Run a "6 - VALVES" cycle. The number of the valves from 1 to 13 is displayed
and each valve is activated once.
Check their correct operations. If a valve is suspected proceed as
described in the procedure : RAS 089 A.

Page 6/7
RAS 092 A Ind.C 10/03/98
 MICROS MECHANIC FUNCTIONS

5 - LCD contrast adjustment

Enter the sub menu "7 - CHG. CONTRAST". Use the arrows to modify the

contrast and press to validate the adjustment.

NOTE : If the LCD screen happens to be unreadable because of a bad contrast

adjustment it is possible to get back a correct contrast pressing at the
same time both keys : + .

6 - Piercing mechanism check (MICROS CT)

Enter the sub menu "8 - PIERCING". Follow the instructions : "PLEASE CLOSE
TUBE HOLDER DOOR" : a piercing operation is simulated.

This function gives the sample tube holder position and the heigth of the
needle in its lower position. See RAS 091 A (MICROS OT) or RAS 121 A
(MICROS CT)

NOTE : If the sample tube holder has been removed the following message is
displayed : "ERROR : NO SAMPLE TUBE HOLDER".
If the sample tube holder has been turned in between two piercing
positions (it means that the tube is not in front of the piercing needle) the
following message is displayed : "ERROR : TUBE HOLDER POSITION'".

* REMARKS

Page 7/7
RAS 092 A Ind.C 10/03/98
 MICROS DRAIN DETECTION ADJUSTMENT

* CONCERNS
Drain detection sensor adjustment/replacement

* REQUIRED TOOLS
- Voltmeter
- Flat screw driver
- 5ml syringe

* REQUIRED PRODUCTS
- None

* INTERVENTION TIME
- 15min

* FREQUENCY
- On request or 1/year

* SPECIFIC KIT OR CONSUMABLES

- Drain detection sensor : XBA 199 A.

Page 1/5
RAS 093 A Ind.D 30/03/00
 MICROS DRAIN DETECTION ADJUSTMENT

* PROCEDURE
The Vacuum/Waste syringe and the chambers drains are controled by an infrared sensor
located below the syringe.
When a drain problem occurs during a cycle two error messages are possible :

- "....." (the instrument carries on the current cycle)

It means that the sensor always detects liquids.
Check the watertightness of the syringe or control the correct adjustment of the cell (see
below)

- "SENSOR ERROR OR MINICLEAN EMPTY" (the instrument stops)

It means that the sensor does not detect liquid transfer before the countings.
Check the Miniclean or diluent level in the containers or control the correct adjustment of the
cell (see below).

* Drain detection sensor adjustment

- Raise the piston up and press the valve <2>.
- Manually perform a syringe drain pressing the valve <5> and pulling down the syringe piston
(Diag.1).

Diag.1

Page 2/5
RAS 093 A Ind.D 30/03/00
 MICROS DRAIN DETECTION ADJUSTMENT
- Make sure the cell is perfectly drained (no bubble).

- Connect a voltmeter between the ground TP30 and TP6 (See Diag.2) and adjust the
voltage to 4,5V + 0,3V by means of R6.

Diag.2

Page 3/5
RAS 093 A Ind.D 30/03/00
 MICROS DRAIN DETECTION ADJUSTMENT

- Fill the syringe up with diluent and connect it on the syringe waste tubing instead of the
syringe nipple (see Diag.3).

Diag.3

- Push diluent through the sensor and check that the voltage falls down below 1Volt on the
voltmeter.

NOTE : Check the commutation from 4,5Volts to ~ 0Volt once again pushing and drawning
alternately liquid and air through the sensor by means of the syringe.

- If nothing happens switch off the instrument and disconnect the connector from J13.
- Replace the drain detection sensor by a new one.
- Switch on the instrument
- Carry out the new sensor adjustment as described above.

Page 4/5
RAS 093 A Ind.D 30/03/00
 MICROS DRAIN DETECTION ADJUSTMENT
* REMARKS
However it is posible to control the correct operation of the sensor by means of the LED
located next to R6 (Upper one).
Indeed the LED should be ON when liquid circulates through the sensor and OFF with air.

Page 5/5
RAS 093 A Ind.D 30/03/00
 MICROS PCB VOLTAGE CHECKS/ADJUSTMENTS

* CONCERNS

- Voltage supply check

- Aperture voltage check
- RBC threshold check/adjustment
- WBC threshold check/adjustment
- PLT threshold check/adjustment
- HGB blank voltage check.
- Stepper motor voltages adjustment

* REQUIRED TOOLS

- Voltmeter
- Flat screw driver

* REQUIRED PRODUCTS

- None

* INTERVENTION TIME

- 20min

* FREQUENCY

- See maintenance chart table.

* SPECIFIC KIT OR CONSUMABLES

- None

Page 1/4
RAS 094 A Ind.E 04/11/1999
 MICROS PCB VOLTAGE CHECKS/ADJUSTMENTS

* PROCEDURE
1 - RBC, PLT, WBC threshold checks/ adjustments

- Ground on TP30.
- Adjust the thresholds according to the below chart table :

THRESHOLDS TEST POINTS VOLTAGE POTENTIOMETERS

WBC TP 10 280 mV + 7 R68
RBC TP13 400 mV + 7 R75
PLT TP16 180 mV + 3 R82

(See Diag.1)

NOTE :
ABC VET : Different threshold for RBC
RBC : 350 mV + 7

2 - Aperture voltage check

See RAS 091 A : Technician functions

3 - Voltage supply check

See RAS 090 A

4 - HGB blank voltage check

See RAS 091 A : Technician functions

5 - Stepper motor voltage checks/ adjustments

- Ground on TP31.
- Adjust the motor voltages according to the below chart table :

MOTORS TEST POINTS VOLTAGES POTENTIOMETERS

Air syringe TP36 2.50 V + 0.05 V R195
Liquid syringe TP35 2.00 V + 0.05 V R186
Horizontal carriage TP37 1.50 V + 0.05 V R177
Vertical carriage TP38 1.00 V + 0.05 V R168

(See Diag.1)

Page 2/4
RAS 094 A Ind.E 04/11/1999
 MICROS PCB VOLTAGE CHECKS/ADJUSTMENTS

Page 3/4
RAS 094 A Ind.E 04/11/1999
 MICROS PCB VOLTAGE CHECKS/ADJUSTMENTS

* REMARKS
The mother board XAA 355 A has been updated to index C according to the following
chart table :

Index A Temperature sensor connected on J14.

Index B 1 - Temperature sensor connected on J11

2 - Fan stand by mode available (twined with the red LED)

Index C Two models of RAM clock usable : RAM DALLAS or SGS.

(Index A & B : RAM SGS only).

The mother board XAA 355 has been updated to the major index B with the stepper
motor voltage adjustments.

Page 4/4
RAS 094 A Ind.E 04/11/1999
 MICROS CT TECHNICIAN FUNCTIONS

* CONCERNS

- HGB photometer calibration

- Aperture voltage check/adjustment
- Pressure check
- WBC gain check/adjustment
- RBC, PLT gain checks/adjustments
- Temperature sensor adjustment
- Cycle numbers
- Version and serial number display.
- Burning
- Sensor adjustment
- Sampling position adjustments
- Vacuum adjustment/check

* REQUIRED TOOLS
- Flat screw driver
- thermometer
- Barflex
- Allen key : 2,5
- Voltmeter

* REQUIRED PRODUCTS
- WBC latex : LAD 001 AS
- RBC and PLT latex : LAD 002 AS
- Soft paper
- Flat piece of stiff plastic

* INTERVENTION TIME
- 60 min

* FREQUENCY
- See maintenance chart table.

* SPECIFIC KIT OR CONSUMABLES

- None

RAS 121 A Ind.D Page 1/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

* PROCEDURE
The arborescence of the «technician functions» is the following :

1 - CAL. PHOTOMETER
1 - WBC ADJUST
1 - VERSION
2 - APERTURE VOLTAGE
2 - # LYM < >
3 - CHECK PRESSURE
3 - # GRA < >
4 - WBC ADJUST
1 - RBC PLT ADJUST

5 - RBC PLT ADJUST 2 - MCV < >

2 - ADJUSTMENTS
1 - NEEDLE SENSOR 3 - MPV < >

6 - SENSOR ADJUST
2 - NEEDLE < >

1 - TRANSFER < >

3 - TRANSFER SENSOR

2 - CHECK
4 - TRANSFER
8 - TECHNICIAN

1 - VACUUM < >

7 - VACUUM
2 - CHECK

8 - SAMPLING POSITION
1 - NEEDLE ADJUSTMENT

1 - UNCAL. TEMPERATURE 2 - NEEDLE 1 < >

3 - TEMPERATURE 2 - CALIBRATE 3 - NEEDLE 2 < >

3 - TEMPERATURE 4 - NEEDLE 3 < >

5 - NEEDLE 4 < >

1 - CONTROL
4 - RUN MODE
6 - NEEDLE 5 < >
2 - USER

1 - YES 7 - NEEDLE 6 < >

5 - REAGENT PACK

2 - NO 8 - PIERCING

6 - SERIAL #
1 - VISIBLE
1 - STARTUP # < >

2 - INVISIBLE
2 - STANDBY # < >
7 - CYCLE #

3 - CBC # < >

1 - BURNING #

8 - BURNING 2 - BURNING
MICROS CT
3 - CONTROL

RAS 121 A Ind.D Page 2/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

Once entering the «SERVICE» sub menu, move to «TECHNICIAN FUNCTIONS» and press .
A specific password (421) is required to enter the sub menus.
Move the cursor by means of and and choose the required menus pressing the
key.

I - The version number is displayed.

II - Adjustments :

1 - HGB photometer calibration

- Dismantle the WBC chamber cover.

- Check that the WBC chamber top is well positioned.

- Check the general cleanliness of the WBC chamber/spectrophotometer assy.

- Re-install the chamber cover.

NOTE - If the WBC chamber has been dismantled previously make sure no liquid has flown
in between the spectrophotomer and the chamber.
Clean the inner surfaces of the spectrophotometer as well as the chamber.
Reassemble the assy and tighten the two screws to the following torque : 400mN/m
(see RAS 088 A : Chamber maintenance)

- Run the CALIBRATION PHOTOMETER function (function 1 of the «ADJUSTEMENTS» menu) :

diluent is delivered to the mixing chamber and transferred to the WBC chamber twice.

An HGB channel is displayed on the LCD screen representing a voltage value as shown below :

VALUE
237

The HGB photometer calibration must be done 20min at least after the
IMPORTANT instrument has been switched on.

- By means of R97 (See diagram 1) adjust the HGB channel according to the room temperature using
the chart table given on next page.

IMPORTANT This adjustment must be done with the WBC chamber cover installed!!!

- Press a key to escape when the adjustment is done.

NOTE After 40 seconds approximately, the function is automaticaly exited.

RAS 121 A Ind.D Page 3/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

MICROS MOTHER BOARD

J38 J39 TP31 TP30 TP29

GND GND GND
J21

RBC COAX WBC COAX

HGB
R97 TP27

TP19

APERTURE R91
VOLTAGE
60V +0.1
-

Diag.1

ROOM CHANNEL VOLTAGE

TPT (°C) Mini. Nominal Maxi. Mini. Nominal Maxi
15 240 245 250 4.71 4.81 4.91
16 240 245 250 4.70 4.79 4.89
17 239 244 249 4.68 4.78 4.88
18 238 243 248 4.66 4.76 4.86
19 237 242 247 4.65 4.74 4.84
20 236 241 246 4.63 4.73 4.83
21 235 240 245 4.61 4.71 4.81
22 234 239 244 4.60 4.69 4.79
23 234 239 244 4.58 4.68 4.77
24 233 238 243 4.56 4.66 4.76
25 232 237 242 4.55 4.64 4.74
26 231 236 241 4.53 4.63 4.72
27 230 235 240 4.51 4.61 4.71
28 229 234 239 4.49 4.59 4.69
29 228 233 238 4.48 4.58 4.67
30 228 233 238 4.46 4.56 4.66
31 227 232 237 4.44 4.54 4.64
32 226 231 236 4.43 4.53 4.62
33 225 230 235 4.41 4.51 4.61
34 224 229 234 4.39 4.49 4.59
Table 1 35 223 228 233 4.38 4.47 4.57

RAS 121 A Ind.D Page 4/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

2 - Aperture voltage

- Once entering the «APERTURE VOLTAGE» menu, connect a voltmeter between the ground (TP30)
and TP19.

- Adjust to 60V + 0,1V by means of R91 (Diagram 1).

The aperture voltage adjustment is only available on the «aperture vol-

IMPORTANT tage» menu. However it is possible to check it during the counting.

- Press any key to escape.

3 - Pressure check

- Disconnect the tube from the vacuum/waste syringe

coming from the valve (see diagram 2).

- Follow the instructions given on the LCD screen :

«PLEASE PLUG BARFLEX ON AIR SYRINGE» (On

the free nipple).

«CHECK PRESSURE : -200mB + 10mB» (The piston

has raised in order to create a vacuum in the syringe
body).

«PLEASE RECONNECT PREVIOUS TUBE»

(disconnect the Barflex and replug the tube instead).

- Check the stability of the vacuum during 30 secon-

des : The vacuum drop down must be < 2 mbar.

- If the results are not correct check the O ring and the
Diag.2 tubing watertightness.

4 - WBC adjust

- Put the WBC latex to mix on a Vortex during 5min or shake thoroughly
As the WBC gain is a factory adjustment it is mandatory not to readjust it
IMPORTANT without taking the following precautions :
Carry out previously an autoconcentrated cleaning to make sure of the
cleanliness of the WBC counting circuit.
If necessary clean the WBC chamber aperture as described in RAS 088 A.
Make sure the Latex has been thoroughly mixed before.

- Run a blank cycle to check the cleanliness of the instrument. Install the vial in the sampling position
required for the blood control and turn the tube holder in the compartment inside position.

CAUTION : Make sure the cap of the vial has been removed.

RAS 121 A Ind.D Page 5/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

- Enter the «WBC ADJUST» sub menu and close the

door of the piercing mechanism (See Diag 3) : A CBC’s
cycle starts.

During the cycle measuring phasis (around 1 minute)

the Lymphocyte and the Granulocyte volumes are
displayed on the screen every 3 seconds as shown
below :

Diag.3

LYM <57 +/- 1> GRA <180 +/- 2>

57 180

- Wait for several results to be displayed and check the stability of both values.

It is mandatory not to operate the gain adjustment as long as the lympho

IMPORTANT and granulo values are not stable.

After one minute the last volume values displayed on the screen are printed out as well as the WBC,
PLT and RBC histograms and the CBC “s results. Check that these printed values correspond to the
following target values :
- LYM = 57 + 1
- GRA = 180 + 2

- If not rerun a «WBC ADJUST» cycle after having previously mixed the latex vial again. By means
of R74 (see diagram 4) adjust the volumes to the target values during the measuring phasis.

MICROS MOTHER BOARD

J38 J39 TP31 TP30 TP29

GND GND GND J21
J11 RBC COAX WBC COAX
Tpt SENSOR

PLT PULSE PROCESS HGB

THRESHOLD TP14 TP25 R97 TP27
TP18 TP26
180mV +3
- TP9 TP24 TP10
R16 R68
R89 R80
R86 R82 TP16 R73 TP19

THRESHOLD
THRESHOLD R75 350mV+5 APERTURE R91
400mV+5 TP13 - VOLTAGE
TP4 - R74 TP7
GAIN
R87 TP15
R81 GAIN 60V +0.1
-
J17 GAIN
TP11

GND TP29
NU
RBC PULSE PROCESS WBC PULSE PROCESS

Diag.4

RAS 121 A Ind.D Page 6/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

5 - RBC PLT adjust

- Put the RBC and PLT latex to mix on a Vortex during 5min or shake thoroughly

As the RBC/PLT gain is a factory adjustment it is mandatory not to readjust

IMPORTANT it without taking the following precautions :
Carry out previously an autoconcentrated cleaning to make sure of the
cleanliness of the RBC/PLT counting circuit.
If necessary clean the RBC/PLT chamber aperture as described in the
procedure RAS 088 A.
Make sure the Latex has been thoroughly mixed before.
- Run a blank cycle to check the cleanliness of the instrument. Install the vial in the sampling position
required for the blood control and turn the tube holder in the compartment inside position.

CAUTION : Make sure the cap of the vial has been removed.

Enter the «RBC PLT ADJUST» sub menu and close the door of the piercing mechanism (Diag.3).
The needle directly delivers the latex sample in the RBC chamber dilution (for a usual analysis cycle
the sample is first delivers to the mixing chamber) and a measuring phasis begins.

- During the cycle measuring phasis (around 1 minute) the Platelet and the Red Blood cell volumes
are displayed on the screen every 3 seconds as shown below :

RBC <74 +/- 1> PLT <59 +/-1>

74 59

- Wait for several results to be displayed and check the stability of both values.

It is mandatory not to operate the gain adjustment as long as the platelet

IMPORTANT and RBC values are not stable.

After one minute the last volume values displayed on the screen are printed out as well as the PLT
and RBC histograms and the CBC “s results.

- Check that these printed values correspond to the following target values :

- RBC = 74 + 1
- PLT = 59 + 1

- If not rerun a «RBC PLT ADJUST» cycle after having previously mixed the latex vial again.

- Adjust the volumes to the target values during the measuring phasis by means of the following
potentiometers (see Diagram 4) :

- RBC gain with R81

- PLT gain with R87

RAS 121 A Ind.D Page 7/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

6 - Adjust sensors

* Needle sensor

If the needle detector 1 (see diagram 5) has been

replaced by a new one or dismantled for any reason, it
is mandatory to re-position it at the right heigth. Proceed
as following :

- Install the piece of plastic 4 (diagram 5) underneath

the piercing needle 3 .

- Once entering the menu «ADJUST SENSORS»

(Selection 6), enter the sub menu «NEEDLE
SENSOR».
- Push the sampling needle 2 downward until it stops
against the piece of plastic and press any key in order
to raise the needle back in its upper position.
The current number of steps, the mini and maxi values
are displayed as well as the way to remove the sensor
Diag.5 (shown by an arrow) if the current value is out of ran-
ges (see below).

CURRENT : 62 MIN : 65 MAX : 75

- For a current number of steps out of ranges, unloosen the 2 screws 5 (diagram 5) and gently move
the sensor - upward if the current value is too low
- downward if the current value is too high.

NOTE 10 steps correspond to around 1 mm. The target number of steps is 70 + 5

- Tighten the screws 5 and rerun a «NEEDLE SENSOR» cycle. Check that the current value is
correct.

RAS 121 A Ind.D Page 8/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

* Needle < >

This adjustment is intended to a factory use. It is mandatory not to readjust

IMPORTANT it without taking the following precautions :
Carry out previously an autoconcentrated cleaning to make sure of the
cleanliness of the RBC/PLT counting circuit.
If necessary clean the RBC/PLT chamber aperture as described in the
procedure RAS 088 A.

This function allows the adjustment of the needle heigth in the RBC chamber. The current number of
steps is 630 and may be adjusted if reproducibilty on RBC/PLT is incorrect.

In this case the value has to be raised 10 steps by 10 steps. Reproducibilty on PLT has to be controled
between each value modification.

Normal ranges : Mini value : 580

Maxi value : 670
Proceed as follow :

- From the SENSOR ADJUST menu, move the cursor to the function <1> "NEEDLE SENSOR" and
press ENTER.

- Push the sampling carriage above the RBC/PLT chamber. Lower the sampling needle until it comes
into contact with the chamber edge as shown on the Diag.6.

Diag.6

- Press ENTER and record the number of step displayed on the LCD. Add 204 to the number displayed and
record the calculated value.

- Move the cursor to the function <2> "NEEDLE" and press ENTER. Enter the calculated value and validate.

RAS 121 A Ind.D Page 9/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

* Transfer sensor

This function allows the adjustment of the carriage

sensor 1 (diagram 7) position.

- Proceed as described below :

- Install the piece of plastic 2 against the left side of

the tube holder compartment 1 (see Diagram 8).

- Once entering the menu «ADJUST SENSORS»

(Selection 6), enter the sub menu «TRANSFER
SENSOR».

Diag.7

Move the carriage 3 on the left until the piercing needle

stops against the piece of plastic. Press any key : the
carriage comes back in its initial position.

The current number of steps, the mini and maxi values

are displayed as well as the way to remove the sensor
if the current value is out of ranges (see below).

- For a current number of steps out of ranges, unloosen

the 2 screws 2 (see diagram 7) and gently move the
sensor

- towards the right if the current value is too low

- towards the left if the current value is too high.
Diag.8

CURRENT : 340 MIN : 345 MAX : 355 >

NOTE 10 steps correspond to around 1 mm. The target number of steps is 350 + 5

- Tighten the screws 2 (see diagram 7) and rerun a «CARRIAGE» cycle. Check that the current value
is correct.

RAS 121 A Ind.D Page 10/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

* Transfer

Once entering the menu «ADJUST SENSORS» (Selection 6), enter the sub menu «TRANSFER».
Then move the cursor to «2 - CHECK» and press ENTER. The needle moves above the RBC chamber.
Check the centering of the needle over the chamber.

If the position of the needle has to be adjust, move to «1 - TRANSFER < >». The value shown on
the screen is the number of steps from its initial position to the RBC chamber position. The current
value is 585.

Press ENTER and raise the value to move the needle towards the left or lower the value to move the
needle towards the right. 10 steps correspond to around 1mm. Rerun a «2 - CHECK» to control the
centering of the needle.

Normal ranges : - Mini value : 520

- Maxi value : 620
- Default value : 575

7 - Vacuum

This adjustement concerns the transfer of the mixing

chamber dilution towards the WBC chamber in order
to insure the HGB stability.

From the menu «ADJUSTMENTS», move to «7 - VA-

CUUM» and press ENTER.
Move to «2 - CHECK» and press ENTER. Liquids are
sent into the mixing chamber and then a chamber drain
begins.

Wait for the «beep» to be triggered off and check to

have liquids remaining in the chamber bottom nipple
(as shown on diag.9) when the «CHECK» cycle is
completed.
If liquids have not been drained correctly, it is possible
to adjust the vacuum value :
Diag.9

Move to «1 - VACUUM < >» and press ENTER. Raise the value if the drain has to be faster. Lower
the value if the drain has to be slower. Press ENTER to confirm. Rerun a «CHECK» cycle to control
the drain of the chamber.

Compare to this «CHECK» cycle , the chamber is totally drained during an analysis
NOTE
cycle.

Normal ranges : - Mini value : 140

- Maxi value : 200
- Default value : 170

RAS 121 A Ind.D Page 11/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

8 - Sampling position

According to the sample tube models (Vacutainers,

Microtainers ...) used and to their position into the
sample tube holder, the heigth of the needle in its lower
position (see diagram 10) can be modified.

The diagram 10 shows two models of tubes used in the

same sampling position :

- Vacutainer and Sarstedt.

Diag.10
The 3 switches associated to the tube holder are able
to detect the sampling position according to the
following principle :

Two states 0/1 are possible for the 3 switches (see diag
11) :

- Switched OFF : 1
- Switched ON : 0

The binary codes obtained from the states of the

switches gives the positions of the tube holder.

For each position of the sample tube holder (from 1 to

6) corresponds a position of the needle (from 1 to 6).
Diag.11

SWITCH 1 SWITCH 2 SWITCH 3 Sampling position Needle

Bad position of the tube
0 0 0 ---
holder
1 0 0 position 1 Needle 1
0 1 0 position 2 Needle 2
1 1 0 position 3 Needle 3
0 0 1 position 4 Needle 4
1 0 1 position 5 Needle 5
0 1 1 position 6 Needle 6

Table 2 1 1 1 No tube holder ---

The code «0 0 0» means that the tube holder has been turned in between two sampling
NOTE positions. The code «1 1 1» means that the tube holder has been removed.

Four of the positions are used on the actual tube holder : (See Diagram 11)

Position 2, Position 4, Position 5, Position 6.

RAS 121 A Ind.D Page 12/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

* Sampling position adjustment

- Move the cursor to the function <1> NEEDLE

ADJUSTMENT and press ENTER.

- Select on the tube holder the position to be

adjusted, place an empty sample tube and close
the sample holder door.

- Manually push the sampling needle to the bottom

of the required sampling position and validate.

- The displays shows the number of step value

which is recorded.

- Press any key to exit the function

Diag.12

The number of step value which is recorded is the maximum value found to the bottom
NOTE
of the position less 3 mm (Diag.12).

It is possible to enter directly the required number of steps for each sampling position. Proceed as
following :

Enter the sub menu that corresponds to the number of the needle : the current number of steps is
displayed. Enter the new value.

Increase the number of steps to move the needle deeper or decrease the value to raise the needle.
Confirm the new value with . The minimum and maximum step values are as follow :

NUMBER OF STEPS
NEEDLE
MINI. DEFAULT MAXI.
1 1 762 782
2 1 630 650
3 1 860 880
4 1 925 945
5 1 1005 1025
Table 3 6 1 580 600

RAS 121 A Ind.D Page 13/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

* Piercing

The piercing function allows to check the piercing operation on each sampling position. From the
SAMPLING POSITION menu, move the cursor to the function <8> Piercing and press ENTER.
Select the required position on the tube holder and close the sample door. The piercing cycle is
carried out and the number of steps for this position is displayed :

NEEDLE 2
CURRENT : 635 STANDARD : 630

III - Temperature adjustment

When entering the «temperature adjustment» menu the following sub menus are displayed :

1 - Uncal. temperature
When pressing the key the sensor temperature value uncalibrated is displayed. This value
should be equivalent to the diluent temperature + 2°.

2 - Calibrate :
The temperature must be calibrated according to the diluent temperature :

- Plunge a thermometer directly into the diluent container and leave it for a while until stabilization.

- Run 2 diluent primes («SERVICE» menu, «PRIME» sub menu, «DILUENT» selection).

- Enter the «CALIBRATE» menu. Note the temperature of the diluent and type in the value (if it is
different from the previous on the instrument).

- Press to save the new value.

3 - Temperature :
1 - Visible : Press to validate the temperature display.
The value displayed is the calibrated temperature.
2 - Invisible : Press to cancel the temperature display.
(Temperature invisible by default)

IV - Run mode

The instrument must be configurated in the «USER» mode (configurated by default in the «USER «
mode), the «CONTROL» mode intends for a factory use.

V - Reagent pack

This function is used to update the instrument from a bottle mode to a pack mode.

RAS 121 A Ind.D Page 14/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

VI - Serial #

Displays the instrument serial number.

VII - Cycle #

Displays the : Startup number since the first use of the instrument.
Stand by number since the first use of the instrument.
CBC number since the first use of the instrument.

The startup, stand by and CBC numbers are adjustable in this menu but
IMPORTANT it is mandatory to keep the initial values (useful for maintenance
schedules).

VIII - Burning

CAUTION : This function which allows the burning of the instrument is intended to a factory
use.

RAS 121 A Ind.D Page 15/16 08/08/96

 MICROS CT TECHNICIAN FUNCTIONS

*REMARKS

RAS 121 A Ind.D Page 16/16 08/08/96

 MICROS CHANGING THE INSTRUMENT LANGUAGE

* CONCERNS
- Changing the instrument language from the V1.2 version.

* REQUIRED TOOLS
- Pair of pliers.

* REQUIRED PRODUCTS
- None

* INTERVENTION TIME
- 5 min

* FREQUENCY
- On request only.

* SPECIFIC KIT OR CONSUMABLES

- None

Page 1/4
RAS 122 A Ind.C 27/10/1999
 MICROS CHANGING THE INSTRUMENT LANGUAGE

* PROCEDURE
- Switch off the instrument.

- Remove both jumpers from E1 (on mother board, see diagram 1).

Diag.1

Page 2/4
RAS 122 A Ind.C 27/10/1999
 MICROS CHANGING THE INSTRUMENT LANGUAGE

- Replug the jumpers on E1 according to the wished language :

JUMPER CONFIGURATION ON E1

ENGLISH

FRENCH

SPANISH

GERMAN

- Switch on the instrument.

Page 3/4
RAS 122 A Ind.C 27/10/1999
 MICROS CHANGING THE INSTRUMENT LANGUAGE

* REMARKS

Page 4/4
RAS 122 A Ind.C 27/10/1999
 MICROS REAGENT PACK

* CONCERNS
- Replacement of the waste connector O ring
- Replacement of the diluent/clean/lyse O rings

* REQUIRED TOOLS
- Pair of pliers

* REQUIRED PRODUCTS
- None

* INTERVENTION TIME
- 15 min

* FREQUENCY
- O ring replacements : 1/year

* SPECIFIC KIT OR CONSUMABLES

- O rings : FAA 036 A

Page 1/3
RAS 123 A Ind.B 09/03/98
 MICROS REAGENT PACK

* PROCEDURE
- Disconnect the pack if this one is still connected.

- Push downward on the reagent pack support as shown on the diagram 1.

Diag.1

- Use a pair of pliers to cut the O rings .

- Replace the O rings by new ones.

- Replace as well the waste connector O ring .

Page 2/3
RAS 123 A Ind.B 09/03/98
 MICROS REAGENT PACK

* REMARKS

Page 3/3
RAS 123 A Ind.B 09/03/98
 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

* CONCERNS
This procedure allows the technician to transform a MICROS OT or CT "bottle" version into a MICROS
OT or CT "pack" version.

1 - This procedure is available only for MICROS having a serial number above 600
WARNING !
as some frame parts are not compatible below this number.
2 - If the MICROS is operating with a program version below V1.3, a specific
password is required to carry out the instrument setup at the end of the procedure.
If this password is unknown, the procedure has to be carried out by authorized
technician only.

* REQUIRED TOOLS
- Hexagonal key 2.5.
- Large and flat screwdriver.
- Cutters.
- Pliers.
- Ruler.

* REQUIRED PRODUCTS
- MICROS reagent packs.

* INTERVENTION TIME
1hour 30minutes

* FREQUENCY
On request

* SPECIFIC KIT OR CONSUMMABLES

- XEA 348 AS : Transformation kit.

RAS 128 A Ind.D Page 1/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

* PROCEDURE

- Remove the tubes from the reagent bottles and

containers, leave the waste tube inside the waste
container.

- Switch on the instrument and run several reagent

priming cycles in order to flush all liquids from the
hydraulic tubes.

- Turn off instrument and remove the power cable

from the main supply and from the instrument.

Diag.1

- Using an hexagonal screwdriver, open the instru-

ment front door (Diag.1).

- Remove the 5 cover fixation screws and remove

the cover (Diag.2).

Diag.2

- From the rear external side of the instrument,

disconnect the diluent and waste tubes from their
connectors (Diag.3).

Diag.3

RAS 128 A Ind.D Page 2/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

- Unscrew the 4 fixation screws (5) of the lower bottle

holder (4) and remove it (Diag.4).

- Unscrew the 3 fixation screws (3) of the bottom

reagent support (1) and remove it.

- Disconnect the ground wiring (2) from the support.

Diag.4

- Disconnect the tubes (Diag.5) :

- 2.29x140 (4) from valve <5> input #1 to waste

connector (3) and remove it.

- 2.29x120 (5) from T connector to diluent input (2)

and remove it.

- From the rear internal side, unscrew the 2 US nuts

(1) from the waste (2) and diluent (3) connectors.
Remove the connectors.

- Disconnect the tube 2.29x1100 from the lyse straw

to the valve <1> input #1 and remove the tube.

- Cut the lyse tube to a length of 610mm.

Diag.5

- Cut the remaining part of the lyse tube to a length of 480mm. This tube will be used for the diluent
output of the reagent pack.

- Disconnect the blue tube from the detergent straw to the valve <4> input #2 and remove the tube.

- Cut the blue tube for the detergent to a length of 510mm.

- Connect a tube 2.29x1100 to the valve <5> input #1. Route the tube from the valve to the top of the
reagent compartment using the previous route of the reagent tubes.

- Remove the reagent bottles from the reagent compartment.

RAS 128 A Ind.D Page 3/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

- Unscrew slightly the top rear panel screw (2) in

order to be able to remove the bottle flap (1) (Diag.6).

- Disconnect the ground wiring (3) from the inside of

the flap.

Diag.6

- Install the flap of the reagent pack (1) and tighten

the fixation screw (2) (Diag.7).

- Re-install the grounding wire (3).

- Install 6 "O" rings 6x1.5 (1) on the 3 connection
pins (Diag.8).

- Stick an adhesive holder below the pack support

between the pin 1 and pin 2 for the future tube route
Diag.7 (See Diag.9).

- Install a shielding wire (5) on each tube and connect

the tubes on the connection pins as follow :

- Pin #1 : blue tube (510mm) going to valve <4>

input #2.
- Pin #2 : tube (610mm) going to valve <1> input #1.
- Pin #3 : tube (480mm) going to the T connector
(valve <7> input #2).

- Install by force the shielding wires (5) on the

connection pins.

- Route the tubes to their final destination using the

hole in the frame of the previous grounding wire.

Diag.8

IMPORTANT Cut the ends of the used tubes to have a better tigthening on the connectors.

RAS 128 A Ind.D Page 4/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"
- Route the tubes of the connection pins 2 and 3
into the adhesive holder, beware not to pinch the
tubes.

- Route the grounding wire to the front of the instru-

ment

- Fix the pack bottom support to the instrument

frame using 3 screws CHC M3x20. Beware not to
pinch the tubes.

- Install 2 "O" rings on the waste cap (7, Diag.8) and

Diag.9 connect the cap on the tube coming from the valve
<5> input #1.

- Install the upper part of the pack support (1)

(Diag.10), by means of a screw CHC M3x5 (2 ) and
the Washer L d=3, jamming the washer L d=3 (7)
between the side panel and the pack support.

- Install the screw CHC M3x8 (8) equipped with the

grounding wire (9), the AZ washer (10) and a washer
L d=3 on the front side aperture.

- Fix the pack side holder plate (5) using 1 screws

FX M3x5 (4), 3 screws FX M3x8 (11), the washer L
d=3 (6) and the nut HM3 (7).

Diag.10
- Install a MICROS reagent pack in its location according to the instructions given on the pack pac-
kage. Check for leaks.

- Reconnect the instrument power cable and switch it ON.

- From the main menu, move the cursor to the function <4> SERVICE and press ENTER. Move the
cursor to the function <8> TECHNICIAN and press ENTER. Enter the technician password (421) and
press ENTER.

- For MICROS instrument operating with a program version below V1.3, a specific password is required.
Move the cursor in front of the hidden function and press ENTER. Enter this password and press
ENTER. Move the cursor next to the function <2> PACK and press ENTER. Move the cursor next to the
function <2> YES and press ENTER. Return to the SERVICE menu with the <ESC> key.

- For MICROS instrument operating with a program version V1.3 or above, move the cursor next to the
function <5> PACK and press ENTER. Move the cursor next to the function <2> YES and press
ENTER. Return to the SERVICE menu with the <ESC> key.

RAS 128 A Ind.D Page 5/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

- From the SERVICE menu, move the cursor next to the function <3> PRIME and press ENTER. Move
the cursor next to the function <1> CHANGE PACK and press ENTER.

The CHANGE PACK function allows a complete priming of the reagent lines and allows
NOTE the initialization of the cycle counter. The PRIME function is recommended only to
prime the reagent lines.

- Follow the displayed instructions and check for leaks. Run an autocleaning cycle from the SERVICE
menu, then several blank cycles.

* REMARKS

* ATTACHED DOCUMENTS

- 1 Pneumatic diagram MICROS OT "PACK" : NMS004C2

- 1 Pneumatic diagram MICROS CT "PACK" : NMS003C2

RAS 128 A Ind.D Page 6/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

RAS 128 A Ind.D Page 7/8 26.06.00

 TRANSFORMATION OF A MICROS
MICROS "BOTTLE" INTO MICROS "PACK"

RAS 128 A Ind.D Page 8/8 26.06.00

 LUBRIFICATION OF THE AIR
MICROS AND LIQUID SYRINGES

* CONCERNS

- This procedure allows the technician to check and to lubricate the air syringe and the liquid syringe
of the ABX MICROS.

* REQUIRED TOOLS
- Hexagonal keys.

* REQUIRED PRODUCTS

- Grease : XEA 381 AS.

* INTERVENTION TIME
- 30 minutes.

* FREQUENCY
- Once a year.

* SPECIFIC KIT OR CONSUMABLES

- Absorbant paper.

RAS 134 A Ind.B Page 1/4 08/10/96

 LUBRIFICATION OF THE AIR
MICROS AND LIQUID SYRINGES

* PROCEDURE

- Switch off the instrument and remove the power

cable.

- Using a 2.5 diameter hexagonal key, remove the

4 cover fixation screws and remove the cover.

- Disconnect the diluent and waste inputs located

at the rear of the instrument.

- Using a small and flat screwdriver, spread a little

amount of grease on the gearings of the air syringe
reductor plate (Diag.1).
Diag.1

- Spread a little amount of grease on the coggs of

the piston axis (Diag.2).

Diag.2

- Move by hand the piston axis up and down in order

to spread the grease all around the gearings and
along the axis (Diag.3).

Diag.3

Place some absorbant paper at the instrument rear connections (diluent and
NOTE
waste) as some liquids may come out when the syringe is pushed.

RAS 134 A Ind.B Page 2/4 08/10/96

 LUBRIFICATION OF THE AIR
MICROS AND LIQUID SYRINGES

- Move the liquid syringe by hand in order to

have an access to the motor gearings.

- Spread a little amount of grease on the gearings

and on the piston axis (Diag.4).

- Move by hand the syringe assembly to spread

the grease on all parts of the gearings and pis-
ton axis.

Diag.4

Place some absorbant paper at the instrument rear connections (diluent and
NOTE
waste) as some liquids may come out when the syringe is pushed.

- Re-install the instrument cover, reconnect the waste and diluent tubes, reconnect the power cable.

- Switch the instrument on and run several priming cycles.

RAS 134 A Ind.B Page 3/4 08/10/96

 LUBRIFICATION OF THE AIR
MICROS AND LIQUID SYRINGES

*REMARKS

RAS 134 A Ind.B Page 4/4 08/10/96

 MICROS LX300 PRINTER SETUP

* CONCERNS
This procedure allows the technician to set up the LX300 printer according to the user's needs.

* REQUIRED TOOLS
- none.

* REQUIRED PRODUCTS
- none

* INTERVENTION TIME
- 15 minutes

* FREQUENCY
- on request

* SPECIFIC KIT OR CONSUMABLES

- none

RAS 138 A Ind.A Page 1/6 04/10/96

 MICROS LX300 PRINTER SETUP

* PROCEDURE

NOTE On the MICROS, it is necessary to select the printer format RESERVED 1 of the
"PRINTER" menu (function 4) accessible through the "OPTIONS" menu (function 5 of
the main menu) then "RESULTS" (function 1).

According to the obsolescence of the CITIZEN printers, a new printer is

IMPORTANT validated : the EPSON LX300. This printer is a 80 column type but with a
printout on 40 columns only. A new program version in the future will
change the result printout presentation and will use the 80 column
possibilities.

Diag.1

RAS 138 A Ind.A Page 2/6 04/10/96

 MICROS LX300 PRINTER SETUP

1 - Printer configuration :

The printer configuration is printed out when pressing the key <FONT> when the printer is switched
ON. The configuration used for the MICROS is the factory configuration :

Diag.2

Each parameter can be modify by the corresponding parameter chart. Each chart is accessible using
the keys <PAUSE>, <FONT> and <LF/FF> according to the control LED combinaisons :

Diag.3

RAS 138 A Ind.A Page 3/6 04/10/96

 MICROS LX300 PRINTER SETUP

2 - Control pannel : (See Diag.4)

Diag.4

The control pannel keys allow the user to set up the main functions of the printer : paper advance,
paper ejection, and font selection. Control LEDS indicate the printer status.

3 - Control LEDS and keys :

LED PAUSE : The orange LED PAUSE lights when the printer stops printing. During each power ON,
this LED blinks for few seconds and 4 audible beeps occur. When the printer runs out of paper, the
LED blinks and 3 audible beeps occur. This LED lights also when the paper is in its tear off position.
When a problem occurs, this LED lights ON and 5 audible beeps occur.

LEDS FONT 1 and FONT 2 : These 2 green LEDS indicate the selected font. Refer to the printer
user's manual to select the font.

Key FONT : During normal operation, the FONT key allows the font selection. For each pressure on
this key, the selection is modified. Refer to the printer user's manual to select the font. When this key
is pressed during the printer power ON, the printer setup menu is entered.

Key LF/FF : During normal operation, a quick pressure on this key allows a ligne feed of the paper.
Keep the pressure on this key to feed a whole page. This key can be used to load or eject the paper.
When this key is pressed during the printer power ON, the printing test starts.

Key PAUSE : When this key is pressed during the printing, the printout stops. Press again on this key
to restart the printout.

PAPER PARK : If Z folded paper is used, the paper can be driven to its parking position when
pressing simultaneously on the keys LF/FF and FONT.

MICRO ADJUST : This function allows to adjust the loading paper position. See the user's manual
for details.

RAS 138 A Ind.A Page 4/6 04/10/96

 MICROS LX300 PRINTER SETUP

4 - Printer description :

Diag.5

Diag.6

RAS 138 A Ind.A Page 5/6 04/10/96

 MICROS LX300 PRINTER SETUP

*REMARKS

RAS 138 A Ind.A Page 6/6 04/10/96

 TRANSFORMATION OF A MICROS
MICROS "PACK" INTO MICROS "BOTTLE"

* CONCERNS
This procedure allows the technician to transform a MICROS OT or CT "pack" version into a MICROS
OT or CT "bottle" version.

* REQUIRED TOOLS
- Hexagonal key 2.5 or torx keys when applicable.
- Cutters.
- Pliers.
- Ruler.

* REQUIRED PRODUCTS
- MICROS reagent bottles.

* INTERVENTION TIME
1hour 30minutes

* FREQUENCY
On request

* SPECIFIC KIT OR CONSUMMABLES

- XEA 447 AS : Transformation kit.

* ATTACHED DOCUMENTS

- 1 Pneumatic diagram MICROS OT "BOTTLES" : NMS002C2

- 1 Pneumatic diagram MICROS CT "BOTTLES" : NMS001C2

RAS 161 A Ind.B Page 1/6 21/07/00

 TRANSFORMATION OF A MICROS
MICROS "PACK" INTO MICROS "BOTTLE"

* PROCEDURE

- Remove the reagent pack from its location, leave

the waste tube inside the waste container.

- Switch on the instrument and run several reagent

priming cycles in order to flush all liquids from the
hydraulic tubes.

- Turn off instrument and remove the power cable

from the main supply and from the instrument.

Diag.1

- Using an hexagonal screwdriver, open the ins-

trument front door (Diag.1).

NOTE : Recent instruments are now equipped with

a lock and a key. Use the key to open the cover.

- Remove the 5 cover fixation screws and remove

the cover (Diag.2).

Diag.2

- Unscrew the 4 screws FHC M3x5 (6) in order to

remove the pack side holder plate (4) (Diag.3).
- Remove the 2 screws CHC M3x5 (5) on the rear
side apertures and the screw CHC M3x10 (3)
equipped with the crosspiece, the grounding wire
and the AZ washer on the front side aperture.

- Remove the pack upper plate (1) and the 2

springs (2).

- Unscrew the 3 screws (7) of the pack support

plate (Diag.4) and remove it.
Diag.3

RAS 161 A Ind.B Page 2/6 21/07/00

 TRANSFORMATION OF A MICROS
MICROS "PACK" INTO MICROS "BOTTLE"

- Disconnect the tubes (1) from the connection pins

(4) as follow (Diag.4) :

- Pin #1 : blue tube (510mm) going to valve <4>

input #2.

- Pin #2 : tube (610mm) going to valve <1> input

#1.

- Pin #3 : tube (480mm) going to the T connector

(valve <7> input #2).

- Remove the shielding wire (2) from each tube

(1).

- Remove the waste cap (6) from the waste tube.

Diag.4

- Reconnect the ground wiring (2) to the bottle sup-

port (Diag.5).

- Screw the 3 fixation screws (3) of the bottom

reagent support (1).

- Screw the 4 fixation screws (5) of the lower bottle

holder (4).

Diag.5

- From the rear internal side, install the diluent and

waste connectors, screw the 2 US nuts (1) to the
waste (2) and diluent (3) connectors (Diag.6).

- Connect the following tubes (Diag.6) :

- Remove the tube 2.29x1100 from valve <5> input

#1 to the reagent pack waste, cut it to 140mm and
connect it (4) to the waste connector (2).

- Remove the tube 2.29x480 from the diluent out-

put of the reagent pack to the T connector, cut it to
120mm (5) and reconnect it to the T connector of
the diluent input (3).
- Remove the tube 2.29x610 from the lyse output
of the reagent pack to the input #1 of the valve
<1>, replace it by a tube 2.29x1100 and connect it
Diag.6 from the lyse straw to the valve <1> input #1.

RAS 161 A Ind.B Page 3/6 21/07/00

 TRANSFORMATION OF A MICROS
MICROS "PACK" INTO MICROS "BOTTLE"

- Remove the tube 2.29x510 from the detergent output of the reagent pack to the input #2 of the valve
<4>. Replace it by a blue tube 2.29x1100 from the output #2 of the valve <4> to the detergent straw.

- Route the tubes from the valves to the top of the reagent compartment using the previous route of
thereagent tubes.

IMPORTANT Cut the ends of the used tubes to have a better tigthening on the connectors.

- From the rear external side of the instrument, place

the label "diluent / waste as shown on the Diag.7.
Connect the waste tube and the diluent tube to their
connectors (Diag.7).

Diag.7

- Install the reagent straws at the end of each tube, waste tube included.

- Install the label on the reagent tube next to the straws : MINICLEAN on the blue tube.

- Place the diluent container at the same level than the instrument, install the MINILYSE and MINICLEAN
into the reagent compartment. Install the bottle stoppers and place the reagent straws.

- Place the waste container below the level of the instrument. Place the rubber stopper and the waste
straw into the container.

- Unscrew slightly the top rear panel screw (2) in

order to be able to remove the reagent pack flap
(1) (Diag.8).

- Disconnect the ground wiring (3) from the inside

of the flap.

Diag.8

RAS 161 A Ind.B Page 4/6 21/07/00

 TRANSFORMATION OF A MICROS
MICROS "PACK" INTO MICROS "BOTTLE"

- Install the flap of the bottles (1) and tighten the

fixation screw (2) (Diag.9).

- Re-install the grounding wire (3).

- Re-install the instrument cover.

Diag.9

- Reconnect the instrument power cable and switch it ON.

- From the main menu, move the cursor to the function <4> SERVICE and press ENTER. Move the
cursor to the function <8> TECHNICIAN and press ENTER. Enter the technician password (421) and
press ENTER.

- For MICROS instrument operating with a program version below V1.3, a specific password is required.
Move the cursor in front of the hidden function and press ENTER. Enter this password and press
ENTER. Move the cursor next to the function <2> PACK and press ENTER. Move the cursor next to
the function <1> NO and press ENTER. Return to the SERVICE menu with the <ESC> key.

- For MICROS instrument operating with a program version V1.3 or above, move the cursor next to the
function <5> PACK and press ENTER. Move the cursor next to the function <1> NO and press EN-
TER. Return to the SERVICE menu with the <ESC> key.

- From the SERVICE menu, move the cursor next to the function <3> PRIME REAGENTS and press
ENTER. Move the cursor next to the function <1> ALL REAGENTS, press ENTER and check for
leaks. Run an autocleaning cycle from the SERVICE menu, then several blank cycles..

NOTE The ALL REAGENTS function allows a complete priming of the reagent lines.

RAS 161 A Ind.B Page 5/6 21/07/00

 TRANSFORMATION OF A MICROS
MICROS "PACK" INTO MICROS "BOTTLE"

* REMARKS

RAS 161 A Ind.B Page 6/6 21/07/00

 BARCODE READER
4 5
• CONCERNS

- Barcode reader installation & configuration: 06/09/99

1 - old model : PANASONIC (P/N : ZE 84 RMD2)
2 - New model : DATALOGIC (P/N : DLC 6065-M)

• REQUIRED TOOLS

None.
M I C R O S

• REQUIRED PRODUCTS

None.

• INTERVENTION TIME

10 minutes.

• FREQUENCY

On request.

• SPECIFIC KIT OR CONSUMABLES

- Installation kits :
PANASONIC : XEA 448 AS
DATALOGIC : XEA 594 AS

RAS 163 A Ind.C

 BARCODE READER

PANASONIC & DATALOGIC READ TEST

4 5
WITH CHECKSUM WITHOUT CHECKSUM
EAN 8 C39

1234567 TEST
M I C R O S

EAN 8
2/5

9076543210

1234567

EAN 13
CODABAR

123456789012
543210

EAN 13

C 128

789:;<=>567=
98765
123456789012

Page 2/5 RAS 163 A Ind.C

 BARCODE READER

* PROCEDURE

MICROS
- Make sure that the BARCODE reader is properly fitted in the DIN plug of the RS adaptor
wiring.

- Make sure that the MICROS version is equal or above V2.3.

- Make sure that the MICROS mother board XAA 355 A has an index H or above (MICROS
standard).

- Make sure that the MICROS mother board XAA 355 B has an index E or above (MICROS
EMC).

- Check the barcode reader works properly by means of the reading test on the previous
page. If the reading test is correct, the procedure is completed. If not, continue below.

Procedure to carry out in case of reading test failure

If the reading test fails, proceed as follows:

- Switch on the instrument and within the 15 first seconds, read from the top to the bottom the
barcode labels located on page 4 (for PANASONIC models) or on page 5 (for DATALOGIC
models) of this procedure. The audible beep occurs after each reading. Read all the labels from
top to bottom and from left to right.

- When the last label is read, the signal beeps 5 times in order to indicate the end of the setup.
Check once more on the test labels.

WARNING
For code I2of 5, to avoid a bad interpretation, it is mandatory to hold the reader in order to read
the entire code.
45

RAS 163 A Ind.C Page 3/5

 BARCODE READER

- If the reading test has failed for the PANASONIC reader, proceed to the barcode setup as
4 5 follows :

Within 15 seconds after switch on :

PANASONIC SETUP

No STF

(5 beeps)
M I C R O S

Setup : default setting No C93

Parity : even C128

1 bit stop Ø

Code identifier : no Ø

Codabar : yes Enter

Exit
No start/stop code

5 Beeps

Page 4/5 RAS 163 A Ind.C

 BARCODE READER

- If the reading test has failed for the DATALOGIC reader, proceed to the barcode setup as
follows :

MICROS
DATALOGIC SETUP
4 low beeps
+1 high
+ 4 low
$+CQ0$-

4 low beeps
+1 high
+ 4 low
$+CP0$-

4 low beeps
+1 high
+ 4 low
$+$*
In case of a reading error, the
barcode reader triggers three
low beeps 4 low beeps

$+

1 beep

CA0CC1AZ0

1 beep

AA1AB11

1 beep

AC110432

1 beep

1 beep
45

AI11

1 beep

EA110DEC0

4 low beeps

$-

RAS 163 A Ind.C Page 5/5

 DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

4 5
• CONCERNS

- Barcode reader installation & configuration DATALOGIC model : DLC 6165-M0

11/10/00

• REQUIRED TOOLS

None.
M I C R O S

• REQUIRED PRODUCTS
None.

• INTERVENTION TIME

10 minutes.

• FREQUENCY

On request.

• SPECIFIC KIT OR CONSUMABLES

- Installation kit : XEA 655 AS

RAS 292 A Ind.A

 DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

4 5 A barcode reader DATALOGIC DLC6165-M0 is available for the MICROS 45 equipped with a
mother board XAA 355 B Ind.E and a software version V2.31 or above.

NOTE : This barcode reader is delivered as an option.

The MICROS 45 barcode reader is set up in order to read the following barcodes :

- C 39 / C 128 / ITF (2/5) / CODABAR

ATTENTION: MICROS having a mother board XAA 355 A modified to index H are also
able to receive the barcode reader

The installation kit XEA 655 AS includes :

- XBA 456 A : 1 barcode reader
- DAC 023 A : 1 reader wiring
- AAK 007 A : 1 EPROM V2.31 or above for MICROS 45
- JAH 006 A : 1 antistatic box
M I C R O S

- CAS 001 A : 1 setup jumper

- RAS 292 A : 1 installation procedure

INSTALLATION

- Switch off the instrument and open the cover.

- Install the jumper (included in the kit) on E10 as shown on the diagram 1.

WARNING !
Once the jumper installed on E10, the instrument data ouput receives 5 volts to supply
the barcode reader. This voltage should cause damages on computer connection if
this one is directly connected on the MICROS 45 data ouput. It is then mandatory to
connect the computer connection only on the cable (DAC 023 AS shown on the diag.2)
intended for it.

- Connect the barcode reader as shown on the Diag.2 and the attached wiring diagram DAC
023 AS (diag 3).

- Install the new EPROM V2.31 software version on U30 (Diag.1) when necessary.

A barcode function has been added into the SETUP menu which allows to valid the checksum
or not according to the type of barcode label in use.

- Control the reading of the barcode reader as described in the following pages.
The barcode setup can be printed out by the function [5] PRINT CONFIG of the menu [3]
SPECIAL from menu [5] SETUP.

Page 2/8 RAS 292 A Ind.A

DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

MICROS MOTHER BOARD

M I C R O S
U30

4 5

Diag.1

RAS 292 A Ind.A Page 3/8

 DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

4 5

Diag 2 (barcode reader picture not contractual)

M I C R O S

Diag 3

Page 4/8 RAS 292 A Ind.A

DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

BARCODE READER TEST AND SETUP

M I C R O S
* PROCEDURE

- Make sure that the BARCODE reader is properly fitted in the DIN plug of the RS adaptor wiring.

- Make sure that the MICROS version is equal or above V2.3.

- Make sure that the MICROS mother board XAA 355 A has an index H or above (MICROS
standard).

- Make sure that the MICROS mother board XAA 355 B has an index E or above (MICROS EMC).

- Check the barcode reader works properly by means of the reading test on the previous
page. If the reading test is correct, the procedure is completed. If not, continue below.

Procedure to carry out in case of reading test failure

If the reading test fails, proceed as follows:

- Switch on the instrument and within the first seconds, read from the top to the bottom
the barcode labels located on page 7 of this procedure. The audible beep occurs after
each reading. Read all the labels from top to bottom and from left to right.

- When the last label is read, the signal beeps several times in order to indicate the end of
the setup. Check once more on the test labels.

WARNING
For code I2of 5, to avoid a bad interpretation, it is mandatory to hold the reader in order
to read the entire code.

BARCODE READER USE

This barcode reader allows the direct entry of barcode identification, sample tubes, calibration
blood lots, QC blood lots on the condition that the instrument is set up with the US identifica-
tion mode (Identification of the sample is displayed and printed out).

- Set the identification mode by means of the SPECIAL FUNCTIONS [7] ID MODE (see User
manual 8.3.7. Identification mode).

Analysis :
- Press the key then carry out the barcode reading. A "beep" is then triggered off and
the patient identification is displayed. Press to valid.
- Run the analysis.
4 5
Quality control :
When the QC run is performed without chip card, and if the sample is identified with a barcode
label, It is possible from the function [2] QC [2] ANALYSIS to directly identify the lot with the
barcode reader.

Calibration :
If the sample is identified with a barcode label, and if the calibration is carried out without chip
card, press the key and read the barcode label.
The sample identification is displayed on the screen. Press to valid : the menu turns to
the expiration date.

RAS 292 A Ind.A Page 5/8

 DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

DATALOGIC DLC 6165-M0 READ TEST

4 5
INTERLEAVED 2 OF 5
CODE 128
(aucun contrôle de Check Digit)
(contrôle sans transmission du check digit)
(no control on Check Digit)
(control without transmission of the check digit)
avec C.D. / with C.D.
M I C R O S

1224488

sans C.D. / without C.D.

121314151617

CODE 39
(aucun contrôle de check digit)
(no control on Check Digit)

sans C.D. / without C.D.

CODABAR
(aucun contrôle d’égalité des caractères start/stop)
(no control of the start/stop character equality)

avec C.D. / with C.D.

37859

12345ABCDEW
123456/$

Page 6/8 RAS 292 A Ind.A

DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

DATALOGIC DLC 6165-M0 BARCODE READER SETUP

M I C R O S
$+CQ0$- Barcode module output mode

$+CP0$- RS232 interface

$+$* Restore system default configuration

$+ Enter configuration environment

CA0CC1CE2 7 data bits, parity even, XON/XOFF

AZ0 Disable all family codes

AB11AB*0ll6 Code39, no C.D. , 1 to 16 char

AC110416 ITF, no C.D. , 4 to 16 char

AD111AD*0316 Codabar, no start/stop, 3 to 16 char

AI11 Code128, C.D. control without transmission

EA110DEC0 Terminator = CR, FIFO disabled

ED3 4 good reads befaore accepting code

$- Exit and save configuration

4 5

RAS 292 A Ind.A Page 7/8

 DATALOGIC DLC 6165-Mo BARCODE READER INSTALLATION

4 5
M I C R O S

E
G
A
P
K
N
A
L
B

Page 8/8 RAS 292 A Ind.A

 5 DATA OUTPUT FORMAT

Table of Content
1. - Message Structure .......................................................................................................................... 4
2. - Details about the structure ............................................................................................................ 4
3. - Identifier list and their formats........................................................................................................ 4
3.1. Hematologic numeric parameters ................................................................................. 4
3.1.1. Format description ............................................................................................ 4
3.1.1.1. Numerical field ..............................................................................................4
3.1.1.2. Parameter status .............................................................................................4
3.1.1.3. Example .........................................................................................................4
3.1.2. Identifier list ........................................................................................................ 5
3.2. Pathology........................................................................................................................... 6
3.2.1. Flags associated with parameters .................................................................. 6
3.2.1.1. Format description .........................................................................................6
3.2.1.2. Identifier list...................................................................................................6
3.2.2. Pathological messages .................................................................................... 6
3.2.2.1. Format description .........................................................................................6
3.2.2.2. Identifier list...................................................................................................7
3.3. Histograms and matrix ...................................................................................................... 7
3.3.1. Format description ............................................................................................ 7
3.3.1.1. Histograms .....................................................................................................7
3.3.1.2. Matrix.............................................................................................................7
3.3.1.3. Separation thresholds .....................................................................................7
3.3.2. Identifier list ........................................................................................................ 7
3.3.3. Format description of the threshold transmission .......................................... 8
3.3.3.1. WBC identifier...............................................................................................8
3.3.3.2. RBC identifier ................................................................................................8
3.3.3.3. PLT identifier.................................................................................................8
3.3.3.4. Basophil identifier..........................................................................................8
3.3.3.5. Lmne matrix identifier....................................................................................9
3.3.3.6. Reticulocyte matrix identifier.........................................................................9
3.4. Patient result identification .............................................................................................. 10
3.4.1. Format description ............................................................................................ 10
3.4.2. Identifier list ........................................................................................................ 10
4. - Packet type ..................................................................................................................................... 11
4.1. Identifiers List ...................................................................................................................... 11
4.2. Data exported by the analyzer....................................................................................... 11
4.3. Errors List.............................................................................................................................. 11
4.4. Data interpreted by the analyzer ................................................................................... 12

RAA 006 A Ind.F Page 5/1 09.03.98

 5 - DATA OUTPUTFORMAT

1. - Message Structure

STX
Size + carriage return.
Identifier followed by a space then the Load Type + carriage return.
Identifier followed by a space then the Information + carriage return.
Remainder of the other Identifiers and Informations + carriage returns.
Identifier followed by a space then the CheckSum + carriage return.
ETX

2. - Details about the structure

Size : 5 bytes representing the total amount of the data except STX and ETX.
Load : A character chain indicating that this load is a result type.
Identifier : 1 byte (moving about $21 to $FF, it describes the information type which follows this
indicator).
CheckSum : Sum modulo 65535 of all the characters except ETX and STX in the hexadecimal
format on 4 bytes.

3. - Identifier list and their formats

3.1. Hematologyc numeric parameters

3.1.1. Format description

3.1.1.1. Numerical field

For all indicated parameters from $21 to $43, the format is a numerical field of 5 digits completed
with zeros on the left side (ex. : 04.55).
The unit is the one chosen by the operator.
When the parameter cannot be calculated by the analyzer, the field is replaced with (--.--).

3.1.1.2. Parameter status

Following the numerical field, a first digit gives the counting rejection status or the suspicion, a
second one gives the parameter value status according to high and low normalities, to high and
low extreme values and to the overloading capacities.
First digit (letter) correspondance
R Parameter rejected for a counting default
B Incorrect balance between the counting
methods
S Suspicious parameter value
'space' No anomaly observed

Second digit (letter) correspondance

L Parameter < to the lower extreme value
l Parameter < to the low normal value
'space' Parameter normal value
h Parameter > to the high normal value
H Parameter > to the high extreme value
O Parameter exceeding the capacity

3.1.1.3. Example
5.5 millions RBC with a counting error in the standard units :
$32$20$30$35$2E$35$30$52$68$0D ou "0 05.5Rh" + carriage return.
The length is fixed and is worth 2+7+1, that is to say 10 bytes for one parameter.

RAA 006 A Ind.F Page 5/2 09.03.98

 5 - DATA OUTPUT FORMAT

3.1.2. Identifier list

Identifiers Parameters Units

$21 ! WBC Standard - SI g/dl - SI
mmoles
$22 " Lymphocytes (#)
$23 # (%)
$24 $ Monocytes (#)
$25 % (%)
$26 & Granulocytes (#)
$27 ' (%)
$28 ( Neutrophils (#)
$29 ) (%)
$2A * Eosinophils (#)
$2B + (%)
$2C , Basophils (#)
$2D - (%)
$2E . Atypical Lymphocytes (#)
$2F / (%)
$30 0 Large Immature Cells (#)
$31 1 (%)

Identifiers Parameters Units

$32 2 RBC Standard - SI g/dl - SI
mmoles
$33 3 HGB
$34 4 HCT
$35 5 MCV
$36 6 MCH
$37 7 MCHC
$38 8 RDW
$39 9 reserved
$3A : reserved
$3B ; Reticulocytes (#)
$3C < (%)
$3D = Reticulocytes Low (%)
$3E > Reticulocytes Median (%)
$3F ? Reticulocytes High (%)

Identifiers Parameters Units

$40 @ PLT Standard - SI g/dl - SI
mmoles
$41 A MPV
$42 B THT
$43 C PDW

Page 5/3
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

3.2. Pathology
3.2.1. Flags associated with parameters

3.2.1.1. Format description

Flags are transmitted in a comprehensive mode (same presentation than on the screen, that is to
say dependant from the language) 2 characters which are replaced with spaces when the flag
has not been detected.
3.2.1.2. Identifier list
Identifiers Parameters Formats Length
$50 P WBC or LMG L1M1M2G1G2G3 2 + 12 + 1
$51 Q Differential CoMbLLNlMnLnRmRnNoNeLb 2 + 22 + 1
$52 R RBC MiMa 2+4+1
$53 S PLT PcScMc 2+6+1

3.2.2. Pathological messages

3.2.2.1. Format description

Each pathology is described by a group of 4 letters preceding a space. The content of the
pathology is dependent from the chosen language. Messages are divided in 3 groupes. Only the
detected pathologies are transmitted. A common header to the 3 groups indicates that the
pathological interpretation has not been determined by the analyzer.
Populations Messages Signification
WBC GRA+ Granulocytosis
GRA- Granulopenia
LEU+ Leukocytosis
LEU- Leukopenia
LYM+ Lymphocytosis
LYM- Lymphopenia
NEU+ Neutrophilia
NEU- Neutropenia
EOS+ Eosinophilia
MYEL Myelemia
LIMC Large Immature Cells
ALYM Atypic Lymphocytes
LSHT Left shift
NRBC Nucleated RBCs
MON+ Monocytosis
RBC ANEM Anemia
ANI1, ANI2, ANI3 Anisocytosis level 1, 2, 3
MIC1, MIC2, MIC3 Microcytosis " "
MAC1, MAC2, MAC3 Macrocytosis " "
HCR1, HCR2, HCR3 Hypochromia " "
POI1, POI2, POI3 Poîkilocytosis " "
ERYT Erythrocytosis " "
PLT THR+ Thrombocytosis
THR- Thrombopenia
AGPL Platelet aggregates
MPEC Small cells
MICC Microcytes
SCHI Schizocytes
All populations ???? No interpretation
PANC Pancytopenia

Page 5/4
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

3.2.2.2. Identifier list

Identifiers Parameters Formats Length

$54 T WBC String of characters 2 + ............ +
1
$55 U RBC String of characters 2 + ........... +
1
$56 V PLT String of characters 2 + ........... +
1

3.3. Histograms and matrix

3.3.1. Format description

3.3.1.1. Histograms
Histograms are transmitted on 128 or 256 channels. They are automaticaly rescaled to a 223
maximum amplitude value. The zero amplitude value is $20, the maximum amplitude value is $FF.

3.3.1.2. Matrix
2048 bytes matrix are transmitted on 4096 bytes giving by group of 2 bytes the value of 1 screen
bitmap byte represented from the left to the right and from the top to the bottom of the screen
(principles of the recovery of the 128x16 matrix bitmap on the VEGA LCD board).

3.3.1.3. Separation thresholds

It is the channel number enclosing areas on the histograms or on the matrix. Each threshold is
transmitted on 3 bytes preceeded by a space (max. : 255).

3.3.2. Identifier list

Identifiers Parameters Formats Length

$57 W WBC Amplitude of each channel 2 + 128 + 1
$58 X RBC " "
$59 Y PLT " "
$5A Z Basophils " "
$5B [ Lmne matrix Screen bitmap 2 + 4096 +1
$5C \ Reticulocyte matrix Screen bitmap 2 + 4096 +1
$5D ] WBC thresholds 5 thresholds 2 + 19 + 1
$5E ^ RBC thresholds 2 thresholds 2+7+1
$5F _ PLT thresholds 1 threshold 2+3+1
$60 ` Basophil thresholds 3 thresholds 2 + 11 + 1
$61 a Lmne matrix thresholds 11 RES thresholds, 6 ABS 2 + 67 + 1
thresholds
$62 b Reticulocyte matrix 2 RES thresholds, 4 ABS thresholds 2 + 23 + 1
thresholds

Page 5/5
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

3.3.3. Format description of the threshold transmission

3.3.3.1. WBC identifier

Separation thresholds 1-2-3 allow the L1 flag determination. For the analyzers in LMG mode, the 4-5
thresholds allow the separation of the 3 populations Lymphocytes, Monocytes, Granulocytes.

Example 1 : output format of the WBC curve thresholds for an LMG sampling. In this example, the
analyzer doesn't send the calculation thresholds of the L1 flag.
"] 000 000 000 040 060" + carriage return.

Example 2 : output format of the WBC curve thresholds for CBC and DIFF sampling. In this 5 parts
DIFF analyzer, the LMG is not measured, the 4-5 thresholds are not significants.
"] 005 008 020 000 000" + carriage return.

1 2 3 4 5
L1

3.3.3.2. RBC identifier

Separation thresholds 1-2 allow the calculation of the microcytic and macrocytic cell proportions.

1 2

%MIC %MAC

3.3.3.3. PLT identifier

The threshold 1 is the number of the last channel used to calculate the PLT number.

PLAQ
1

3.3.3.4. Basophil identifier

Thresholds 1-2-3 allow the determination of the basophil proportion regarding the total number of
WBCs.

1 2 3

BASO

Page 5/6
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

3.3.3.5. Lmne matrix identifier

The 11 resistive thresholds are transmitted in the following order : Nol, Non, Noe, Ln, Rn, Ll, Al, Lm,
Lmn, Mn, Rm. The 3 absorbance thresholds are following : Nl, Ne, Rmrn.
At the end the width of the areas (in channel number) describing the proximity flags : Fne, Fmn, Fln
are transmitted .
Caption :
The position of the miscellaneous populations given in the differential are shown in italic.
Framed items are the proximity flags.
Surrounded items are inflexion points similar to separation thresholds.
AC NoE

NOISE EOS

FNE

NoN
NE

NEUTRO RN
LN

RMN

MONO
NL

LYMPHO

DC
NoL LL AL LM RM
FLN

FMN LMN MN

3.3.3.6. Reticulocyte matrix identifier

The 2 resistive thresholds are transmitted in the following order :R1, R2.
Then the 4 fluorescent thresholds are following : F1, F2, F3, F4.
Fluorescence

F4

F3

F2

F1

DC
R1 R2

Page 5/7
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

3.4. Patient result identification

3.4.1. Format description

All the described fields have a fixed size character chain type and are completed with spaces for
the non significant informations.

3.4.2. Identifier list

Identifiers Correspondance Formats Length

$70 p Analyzer number 01 2+2+1
$71 q Analysis date and time 94/06/06 13h15mn31s 2 + 19 + 1
$72 r Analyzer run number 94/06/06-0001001 or 0115 2 + 16 + 1
$73 s Analyzer sequence number 0128 2+4+1
$74 t Sampling mode 'O' : open tube 2+1+1
'C' : close tube
$75 u Identification number 1450302154275-42 2 + 16 + 1
$76 v Identification SMITH Ronald 2 + 30 + 1
$77 w Birth date yy/mm/dd 2+8+1
$78 x Age 7d, 4w, 10m, 54y 2+3+1
$79 y Sex 2 2+1+1
$7A z Origin F 2+1+1
$7B { Doctor Dr; Jones 2 + 15 + 1
$7C | Department Hemato 2 + 10 + 1
$7D } Sampling date 94/06/06 13h15 2 + 14 + 1
$7E ~ Comments 2 + 32 + 1
$7F Blood type Cat 2 + 16 + 1
$80 ç Analysis type 'C' : CBC 2+1+1
'F' : CBC & Differential
'R' : Reticulocytes
'L' : LMG
$81 ü Sample rack type '0' : 10 positions 2+1+1
'1' : 15 positions
'2' : 32 positions
$82 é Number of runs '0' : first run 2+1+1
'1' : second run etc...

Page 5/8
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

4. - Packet type

The information described in the packet type allows the specification of the global message
content; Analyzers being able to communicate in the bidirectionnal mode and supporting the
remote control mode, this string of characters can be presented under several forms. Its length is 8
characters.
The version number is linked to the development of the hematological message identifiers.
An error number is also available. It codifies some events which happened on the analyzer (see
error number list here below).
4.1. Identifiers List

Identifier Correspondance Format Length

$FC Error number 4 decimal bytes 2+4+1
$FD 16 bits check sum value 4 hexadecimal bytes 2+4+1
$FE Version N° of Identifier list String of characters : Vx.xx 2+5+1
$FF type of data packet String of characters 2+8+1

4.2. Data exported by the analyzer

Data packet string (8 Use

char.)
RESULT Hematological result transmission on a routine mode
RES-RR Hematological result transmission on a re-sampling mode
RES-BLK Result transmission on a blank sampling mode
QC-RES-H Result transmission of a high level control blood
QC-RES-M Result transmission of a median level control blood
QC-RES-L Result transmission of a low level control blood
QC-TAR-H Target transmission of a high level control blood
QC-TAR-M Target transmission of a median level control blood
QC-TAR-L Target transmission of a low level control blood
FILE Patient file request
LINE Connection request
END End of the connection
BUSY Analyzer not available
WHAT? Response to a non registered request

4.3. Errors List

Error N° event linked to the analyzer
0001 Operating temperature out of limits

Page 5/9
RAA 006 A Ind.F 09/03/98
5 - DATA OUTPUT FORMAT

4.4. Data interpreted by the analyzer

Data packet string (8 char.) Use

FILE Patient file reception
AUTO Start of an automatic sampling mode (piercer)
STEP Start of an automatic sampling mode (piercer) with the
choice of the tube position number to be sampled
NO:xx Sampling request of a tube in the position xx: 01 to 32
MANUAL Start of a manual sampling mode (open tube)
STOP Emergency stop
STOPAUTO Stop of an automatic sampling cycle (piercer)
DILUENT Diluent rinse cycle
LYSE Lyse priming cycle
CLEANER Cleaning cycle
EOSINO Eosinofix priming cycle
BASO Basolyse priming cycle
RETIC Retix priming cycle
CLRNEEDL Needle cleaning cycle
BCKFLUSH Backflush cycle
DRAIN Drain chamber cycle
CONTROL Autocontrol cycle
STARTUP Startup cycle
SHUTDOWN Shutdown cycle
SETCBC Setup in the CBC analysis mode
SET5D Setup in the Differential analysis mode
SETRET Setup in the Reticulocytes analysis mode
SETLMG Setup in the LMG mode
REMOTE Run in the remote control mode
STANDARD End of the remote control mode

Page 5/10
RAA 006 A Ind.F 09/03/98
 5 - DATA OUTPUT FORMAT

2. PIN ASSIGNMENTS

COMPUTER
MICROS SIDE
RS 232 OUTPUT DB9 CONFIGURATION

5 GND GND 5
6 4 6
4
7 TXD TXD 3 7
3
8 RXD RXD 2 8
2
9 9
1 1

COMPUTER
SIDE
DB25 CONFIGURATION

13
MICROS
RS 232 OUTPUT

5 GND GND 7
6
4
7 TXD RXD 3
3
8 RXD TXD 2
2
9
1 1

Page 5/11
RAA 006 A Ind.F 09/03/98