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This document discusses genetic analysis of two plant varieties (AVeBrJ and GOA) and their hybrids. Principal coordinate analysis grouped the varieties into distinct clusters, with hybrids in an intermediate position. Structure analysis identified two genetic populations corresponding to the two varieties. Further Structure analysis of F2 hybrids ordered them according to their inherited genomic proportions from each parental variety, with most individuals showing intermediate inheritance.
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This document discusses genetic analysis of two plant varieties (AVeBrJ and GOA) and their hybrids. Principal coordinate analysis grouped the varieties into distinct clusters, with hybrids in an intermediate position. Structure analysis identified two genetic populations corresponding to the two varieties. Further Structure analysis of F2 hybrids ordered them according to their inherited genomic proportions from each parental variety, with most individuals showing intermediate inheritance.
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Figure 2. Principal coordinate analysis based on genetic distances estimated by sharing alleles between
GOA, AVeBrJ, and F1 Hybrid samples. The samples were projected in this two-dimensional space in four
distinct groups. (AVeBrJ) in Blue; (GOA) in Red, subdivided into two groups and the PB-141 hybrids in
Purple, in an intermediate position between the parental varieties.

Figure 3: Graph generated by Structure Harvester (Evanno et al. 2005) showing the value of ΔK = 2 most
consistent with the genetic structure observed in the set of individuals analyzed.

Figure 4: Analysis of the genetic structure carried out with the Structure software of two populations of
AVeBrJ and GOA, and F1 and F2 plants from the crossing between the two varieties. Grouping with K=2,
K=3, K=4 and K=5 are presented. The numbers indicate the groups: BREW (1); GOA group 1 (2); GOA
group 2 (3); Hybrids F1 (4); Hybrids F2 (5).

In addition, the F2 plants were visually classified according to the genomic proportion
inherited from the AVeBrJ, using the Structure Plotsoftware, which allows the use of the data
generated by the Structure thus providing a sorting of F2 hybrids according to the highest
proportion inherited from the AVeBrJ genome in descending order (Figure 5). As expected, the
F2 hybrids had well-distributed AVeBrJ and GOA genomic proportions, with a majority of
individuals presenting intermediate genomic proportions of each variety.
Figure 5: Hybrids F2 (5) ordered according to the AVeBrJ (green) and GOA (red) genome proportions
obtained from the analyses performed with Structure. A. Genomic proportions of all analyzed groups,
with a higher genomic proportion of JVBV ordered only in F2 hybrids (5). B. Genomic proportions
ordered decreasingly from the highest genomic proportions of JVBV displayed by the F2 hybrids (5).

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