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Green Synthesis of Silver Nanoparticles

The document summarizes an experiment that tested the antimicrobial activity of silver nanoparticles synthesized using Polygonum glabrum Willd. leaf extract. Bacteria cultures were exposed to varying concentrations of the nanoparticles, and inhibition zones were measured. UV-Vis spectroscopy showed the nanoparticles exhibited surface plasmon resonance at 429 nm, indicating they were spherical and uniform in size. The nanoparticles demonstrated antimicrobial activity against both gram-positive and gram-negative bacteria.

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Afrah M
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37 views2 pages

Green Synthesis of Silver Nanoparticles

The document summarizes an experiment that tested the antimicrobial activity of silver nanoparticles synthesized using Polygonum glabrum Willd. leaf extract. Bacteria cultures were exposed to varying concentrations of the nanoparticles, and inhibition zones were measured. UV-Vis spectroscopy showed the nanoparticles exhibited surface plasmon resonance at 429 nm, indicating they were spherical and uniform in size. The nanoparticles demonstrated antimicrobial activity against both gram-positive and gram-negative bacteria.

Uploaded by

Afrah M
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Polygonum glabrum Willd. leaf extract mediated green synthesis of silver nanoparticles and their ..

Antimicrobial activity: Antimicrobial activity of biogenic silver nanoparticles was assessed by well diffusion method against
gram negative (Escherichia coli MTCC 723, 1554, Pseudomonas aeruginosa MTCC 736 and Salmonella typhi MTCC3216) and
gram positive (Staphylococcus aureus MTCC3160) microorganisms.
Articl Nano Biomed.
e Eng.
Initially, the stock cultures of bacteria were revived by inoculating broth media and grown at 37ºC for 18
hrs. The agar plates were poured by muller hinton media and wells were made in the plate. Each plate
was inoculated with 18 h old cultures (100 μl, 10 -4cfu) and spread evenly on the plate. After 20 min, the
wells were filled with desired quantity (20, 40, 60, 80 and 100 μl) of nanoparticles solution. All the plates
were incubated at 37ºC for 24 h and the diameter of inhibition zone was noted.

I. Results and discussion


When leaf extract of P.glabrum was added to aqueous 1mM silver nitrate solution which
leads to the appearance of dark brown colour within 10 min. The colour change was arising
due to the property of quantum confinement which is a size dependent property of
nanoparticles which affects the optical property of the nanoparticles 30. UV-Visible
absorption spectroscopy is widely used to study the optical properties and electronic
structure of nanoparticles. The process of bio reduction of silver ions was gradually
monitored in UV- Vis spectroscopy Figure 2 depicts a series of absorption spectra recorded
from the solution of silver nanoparticles at different time intervals. The absorption spectra
showed an intense peak at 429 nm due to the surface Plasmon resonance (SPR) band of
silver nanoparticles. Noble metals exhibit unique optical properties due to the property of
surface plasmon resonance (SPR) which is the collective oscillation of the conduction
electrons induced by the interacting electromagnetic field. The size and shape of metal
nanoparticles determine the spectral position of plasmon band absorption as well as its
width 31 .The appearance of single prominent peak shows that the particles are spherical and
uniform in size 32 which is further confirmed by SEM photograph

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