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Determination of Yield of Silver Nanoparticles Using Atomic Absorption Spectroscopy (AAS)

The document discusses two studies: 1) Using atomic absorption spectroscopy (AAS) to analyze the conversion of silver ions to silver nanoparticles (AgNPs) during synthesis, finding a 97.14% yield after 20 minutes of centrifugation. 2) Testing the antibacterial activity of synthesized AgNPs against E. coli and S. aureus, finding the highest activity against E. coli and the lowest against S. aureus, with increased effectiveness compared to silver nitrate due to the AgNPs' larger surface area and ability to damage cell membranes and DNA.

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87 views2 pages

Determination of Yield of Silver Nanoparticles Using Atomic Absorption Spectroscopy (AAS)

The document discusses two studies: 1) Using atomic absorption spectroscopy (AAS) to analyze the conversion of silver ions to silver nanoparticles (AgNPs) during synthesis, finding a 97.14% yield after 20 minutes of centrifugation. 2) Testing the antibacterial activity of synthesized AgNPs against E. coli and S. aureus, finding the highest activity against E. coli and the lowest against S. aureus, with increased effectiveness compared to silver nitrate due to the AgNPs' larger surface area and ability to damage cell membranes and DNA.

Uploaded by

Afrah M
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

Determination of yield of silver nanoparticles using atomic absorption spectroscopy


(AAS)

AAS analysis was carried out to analyze unreacted Ag + ion concentration, which shows the
conversion of Ag+ ions into AgNPs (Choudhary et al., 2016).
The conversion of Ag+ to AgNPs and percentage yield of AgNPs increases with increasing
centrifugation time. From the point of view of time and energy, 20 min of centrifugation time is
selected. In the present study, it is comparatively high yield of AgNPs (97.14%) and took about 20
min of centrifugation which is quite more than yield of AgNPs (87%) reported in the literature
(Mehmood et al., 2016).

Antibacterial activity of silver nanoparticles

Experimental results are expressed as mean ± standard deviation (SD). Biosynthesized AgNPs
shows the highest antibacterial activity against E. coli (38.00± 0.61 mm, Figure 8a) in 100 µg/ml and
the lowest antibacterial activity against S. aureus (11.00± 0.41 mm, Figure 8a) in
25 µg/ml. Silver nitrate solution shows the highest antibacterial activity against E. coli (28.00±0.41
mm, Figure 8b) in 100 µg/ml and the lowest antibacterial

DOI: 10.9790/3008- g 1 | Page


IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

activity against S. aureus (9.00 ± 0.58 mm, Figure 8b) in 25 µg/ml. When antibacterial
activity of silver nitrate and AgNPs are compared against the studied bacteria, an increase in
antibacterial activity of AgNPs over the use of silver nitrate is observed. Similarly, it has been
reported that AgNPs show higher antibacterial activity than silver nitrate (Marslin et al.,
2015).
The high surface area to volume ratio of AgNPs increases their contact with micro-
organisms, promoting the dissolution of Ag + ions and hence improving biocidal effectiveness.
Formation of free radicals by the AgNPs when in contact with the bacteria, and free radicals
have the ability to damage the cell membrane and make it porous which can ultimately lead
to cell death (Tenzin et al., 2016). Silver is a soft acid, and there is a natural tendency of an
acid to react with a base; in this case, a soft acid to react with a soft base. Another fact is
that the deoxyribonucleic acid (DNA) has sulfur and phosphorus as its major components;
AgNPs can act on these soft bases and destroy the DNA which would definitely lead to cell
death (Masoud et al., 2016).

DOI: 10.9790/3008- www.iosrjournals.or 2 | Page

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