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Qubit 4 Fluorometer-Fast Answers For Precious Samples: Find Out If You Have Enough DNA or RNA For Your Experiment

The document describes the Qubit 4 Fluorometer, which can quickly quantify DNA or RNA using small sample volumes. It uses selective dyes to bind to targets and minimize effects of contaminants. Kits are available for quantifying different biomolecule types like DNA, RNA, and proteins. The fluorometer provides fast, reliable results and flexible export options.

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100% found this document useful (1 vote)
271 views2 pages

Qubit 4 Fluorometer-Fast Answers For Precious Samples: Find Out If You Have Enough DNA or RNA For Your Experiment

The document describes the Qubit 4 Fluorometer, which can quickly quantify DNA or RNA using small sample volumes. It uses selective dyes to bind to targets and minimize effects of contaminants. Kits are available for quantifying different biomolecule types like DNA, RNA, and proteins. The fluorometer provides fast, reliable results and flexible export options.

Uploaded by

misuakechi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Qubit 4 Fluorometer—fast

answers for precious samples


Purify DNA Purify RNA Quantitate Analyze
Find out if you have enough DNA or RNA
for your experiment

The Invitrogen™ Qubit™ 4 Fluorometer with Wi-Fi is designed DNA HS


to quickly and specifically quantitate DNA or RNA.
DNA BR

ssDNA
Key features include:
• Invitrogen™ Qubit™ assay dyes bind selectively to miRNA
DNA, RNA, or protein, making it more sensitive than RNA BR
UV absorbance
RNA HS

• Uses as little as 1 μL of sample, even with very UV


dilute samples
10–3 10–2 10–1 10 101 102 103 104

• Fast, reliable detection of degraded RNA with the Quantifiable range (µg/mL)
Invitrogen™ Qubit™ RNA IQ Assay Figure 1. Comparison of quantifiable sample concentration
ranges for the Qubit assays vs. UV absorbance measurements.
• Integrated reagent calculator to quickly generate working UV absorbance readings are not selective for RNA vs. DNA.
solution calculations

• Flexible options for exporting results: Wi-Fi, USB drive, or


direct connection with a USB cable
Features of Qubit assays
• Kits contain concentrated assay reagent, dilution buffer, • All assays are performed using the same general
and prediluted standards protocol with a simple mix-and-read format

• Dyes fluoresce only when bound to the target of interest, • Only 2 minutes of incubation time required for DNA and
minimizing the effects of contaminants—including RNA assays
degraded DNA or RNA—on the result

Ordering information
Product Initial sample concentration Quantitation range Quantity Cat. No.
RNA integrity and quality kit
75 assays Q33221
Qubit RNA IQ Assay Kit* NA NA
275 assays Q33222
RNA quantitation kits
100 assays Q10210
Qubit RNA BR Assay Kit 1 ng/µL to 1 µg/µL 20–1,000 ng
500 assays Q10211
100 assays Q32852
Qubit RNA HS Assay Kit 250 pg/µL to 100 ng/µL 5–100 ng
500 assays Q32855
100 assays Q32880
Qubit microRNA Assay Kit 50 ng/mL to 100 µg/mL 1–1,000 ng
500 assays Q32881
DNA quantitation kits
Qubit ssDNA Assay Kit 50 pg/μL to 200 ng/μL 1–200 ng 100 assays Q10212
100 assays Q32850
Qubit dsDNA BR Assay Kit 100 pg/μL to 1,000 ng/μL 2–1,000 ng
500 assays Q32853
100 assays Q32851
Qubit dsDNA HS Assay Kit 10 pg/µL to 100 ng/µL 0.2–100 ng
500 assays Q32854
100 assays Q33230
Qubit 1X dsDNA HS Assay Kit 10 pg/µL to 100 ng/µL 0.2–100 ng
500 assays Q33231
Protein quantitation kit
100 assays Q33211
Qubit Protein Assay Kit 12.5 µg/mL to 5 mg/mL 0.25–5 μg
500 assays Q33212
Instrument and accessories
Qubit 4 System Verification Assay Kit 1 kit Q33237
Qubit 4 Fluorometer with Wi-Fi 1 instrument Q33238
Qubit 4 Quantitation Starter Kit (with Wi-Fi) 1 kit Q33239
Qubit 4 NGS Starter Kit (with Wi-Fi) 1 kit Q33240
Qubit 4 RNA IQ Starter Kit (with Wi-Fi) 1 kit Q33241
Qubit Assay Tubes 500 tubes Q32856
* Note: The Qubit RNA IQ Assay for the detection of degraded RNA can only be run on the Qubit 4 Fluorometer and cannot be performed on the original Qubit, Qubit 2.0, or Qubit 3.0 Fluorometers.

Find out more at thermofisher.com/qubit


For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. COL18224 0119

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