HISTOPATHOLOGY LECTURE LECTURE NOTES IN STAINS (1)
GLYCOGEN, CONNECTIVE TISSUE-COLLAGEN, ELASTIC FIBERS, RETICULUM, BONE AND CARTILAGES
TABLE OF HISTOPATH STAINS
STAINING TECHNIQUE SPECIMEN/SUBSTANCE FIXATION REAGENTS RESULT
Best Carmine Method Glycogen Absolute Alcohol or Carnoy’s Carmine Stain; Ammonia Water, Glycogen-brilliant red
Fluid Methyl Alcohol
Bauer Feulgen Reaction Glycogen 9 parts of Absolute Alcohol, 1 Chromic acid, HCL, Feulgen Glycogen- red to red purple
part of Formalin leukofuchsin, Sodium Bisulfate,
Harris Hematoxylin
Periodic Acid- Schiff Glycogen Glycogen 10% formalin or Zenker’s Coleman’s Feulgen rgt or Schiff Glycogen-magenta red
Reaction (PAS) solution leukofuschin 0.5% periodic acid
HCL Harris Hematoxylin
Langhan’s Iodine Method Glycogen Formalin Gram’s Iodine Glycogen-mahogany brown
Mallory’s PTAH Method Connective tissue-collagen Zenker’s fluid or formalin PTAH Collagen-yellowish to brownish
red
Mallory’s aniline Blue Method Connective tissue-collagen Zenker’s fluid Acid Fuchsin solution Aniline Collagen-blue
Blue-Orange G solution
Mallory’s phosphomolybdic acid Connective tissue-collagen Zenker’s fluid Phosphomolybdic acid Collagen-blue
Hematoxylin Hematoxylin solution
HPS Trichome Stain Method Connective tissue-collagen Picric acid fixation Hemalum phloxine Saffron Collagen-yellow orange
Heidenhain Iron Hematoxylin Contnective tissue-collagen Zenker’s fluid Ammonio-Ferric Alum Collagen-blue
Method Hermatoxylin Van Gieson stain
Massons Trichome Method Connective tissue-collagen Bouin’s fluid or 10% formalin Weigert’s Hematoxylin Bilbrich Collagen-blue
Scarlet acid fuschin solution.
Phosphomolybdic acid
phosphotungstic acid solution
Aniline blue solution light green
solution 1% acetic acid water
solution
Barbeito-Lopez Trichome Connective tissue-collagen Acetic acid fuchsin Collagen-brilliant geen
Method Acetic a cid formalin molybdic
acid aniline blue-methyl orange
solution
Van Gieson Picrofuchsin Method Connective tissue-collagen Any Fixation Harris Hematoxylin or Celestine Collagen-Bright red
blue Van Gieson picrofuschin
HISTOPATHOLOGY LECTURE LECTURE NOTES IN STAINS (1)
GLYCOGEN, CONNECTIVE TISSUE-COLLAGEN, ELASTIC FIBERS, RETICULUM, BONE AND CARTILAGES
Gellego’s Iron Fuschin Method Connective tissue-collagen 10% Formalin Iron chloride solution, Weigert’s Collagen and reticulum- deep
Hematoxylin, 0.1 % acetic acid, blue
carbol fuschsin aniline blue-picric
acid solution
Lillie’s Biebrich scarlet-Picro Connective tissue-collagen Any Fixation Weigert;s iron chloride Collagen-blu
Aniline Blue Method Hematoxylin Biebrich scarlet in1
% Hac Aniline Blue Hac
Methanolic Luxol Past Blue Collagen and elastin 10% Formalin Luxol fast blue G methanol aq. Collagen and elastic fibers-bright
Tartrazine blue
Snook’s Reticulum Method Reticulum 10% Formalin Potassium permanganate oxalic Reticulum-black
acid solution uranium nitrate, 1%
Gold chloride nuclear fast red
Silver nitrate, Sodium nitrate;
Sodium hydroxide, Sodium
thiosulfate
Laidlaw’s Silver Impregnation Reticulum Any Fixative Potassium permanganate, 5% Reticulum-violet to black
Method oxalic acid; Ammoniacal silver
carbonate, Gold chloride Alum
hematoxylin Formalin, Van
Gieson stain , Sodium Thiosulfate
Gordon and Sweet’s Silver Reticulum Any Fixative Potassium Permanganate oxalic Reticulum- brownish black or
Impregnation acid Wilder’s SilveSolution, Gold purple
Chloride 10% formalin solution
thiosulfate
Weigert’s Resorcin-Fuhsin Elastic Elastic Fibers Any Fixative Weigert Hematoxylin Resorcin- Elastic fibers- blue black to black
Statin Method fuchsin solution, Van Gieson
Solution
Verhoeffs Elastic Stain Elastic Fibers Any Fixative Hematoxylin Ferric Chloride, Van Elastic fibers- blue black to black
Gieson Stain, Sodium Thiosulfate
Unna- Taenzer Acid Orcein Stain Elastic Fibers Any Fixative HCL-Orcein solution, Van Gieson Elastic fibers- brownish black or
picro fuchsin H and E Stains dark brwn
Gomori’s Aldehude Fuchsin Stain Elastic Fibers Any Fixative Aldehyde Fuchsin Solution, Vam Elastic Fibers- deep blue to
HISTOPATHOLOGY LECTURE LECTURE NOTES IN STAINS (1)
GLYCOGEN, CONNECTIVE TISSUE-COLLAGEN, ELASTIC FIBERS, RETICULUM, BONE AND CARTILAGES
Gieson picrofuchsin stain purple
KOneff’s Stain Bone and Cartilage Zenker-solution Iron alum solution, Methyl green
solution. Aniline blue solution,
Harris Solution.
Phosphomolybdic acid
