Name: Destine Cristel Reyes
Grade and Section: 12-CSS
Laboratory Work 1
Spontaneous Generation of Maggots
Materials
3 bottle jars (same size)
3 slices of fresh meat (pork, beef, chicken) or fruits (mashed potato, banana, papaya) as a substitute
Clean stockings or gauze
Rubber bands
Procedures
1. Clean and dry three glass jars or bottles
2. Put a piece of fresh meat or fruit at the bottom of the three jars.
3. Keep the first jar open and leave it outside the room where it will be exposed to flies. Label this
Jar A.
4. Seal the mouth of the second jar with clean stockings or gauze using a rubber band. Keep it
beside Jar A. Label this Jar B.
5. Tightly put the cap and seal the third jar. Keep it inside the laboratory room (dry area). Label this
Jar C.
6. Observe the appearance of maggots after several days to two weeks. Continue the observation
until you observe the growth of maggots.
7. Record your observations.
Observations
DAY 1 (09/15/23)
Jar A Jar B Jar C
Growth of Maggots None None None
Smell Normal Normal Normal
Color of the meat or fruit Yellow Yellow Yellow
Growth of Fungi None None None
DAY 2 (09/16/23)
Jar A Jar B Jar C
Growth of Maggots None None None
Smell Slightly foul odor Normal Normal
Color of the meat or fruit Yellow (with some Yellowish Yellowish
brown spots)
Growth of Fungi Little None None
DAY 3 (09/17/23)
Jar A Jar B Jar C
Growth of Maggots Little (but small None None
maggots)
Smell Overripe banana Slightly foul odor Normal
Color of the meat or fruit Brownish Yellow (with some Yellowish
brown spots)
Growth of Fungi It’s getting bigger Little Little
DAY 4 (09/18/23)
Jar A Jar B Jar C
Growth of Maggots The small maggots None None
increased (they look
like tiny bubbles)
Smell The smell is Overripe banana Slightly foul odor
unbearable
Color of the meat or Brownish (with some Brownish Yellow (with some
fruit black spots) brown spots)
Growth of Fungi The fungus increased It’s getting bigger It’s getting bigger
compared to day 3 (color yellow, green,
and some white
molds)
DAY 5 (09/19/23)
Jar A Jar B Jar C
Growth of Maggots The small maggots None None
increased compared
to day 4 (but there are
some big maggots)
Smell The smell is
unbearable
Color of the meat or Brownish like day 4
fruit (but the black spots
get bigger)
Growth of Fungi The molds are gone
(but replaced by small
maggots)
DAY 6 (09/20/23)
Jar A Jar B Jar C
Growth of Maggots
Smell
Color of the meat or fruit
Growth of Fungi
(ATTACHED PHOTO HERE)
Post-Lab Questions:
1. What is a controlled experiment?
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2. What are the manipulated variables in this experiment?
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3. Why do you observe the growth of fungi in all setups after several days of the experiment?
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Note: Please provide pictures of your activity, this will serve as a proof.
