Drugs of Abuse Screen (250 Analytes) in

Urine by LC-MS/MS
Application #AN4140
Page 1 of 4
Conditions
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL-1010-1002U
Mobile Phase: A: 2 mM ammonium acetate + 0.1% formic acid in H2O
B: 2 mM ammonium acetate + 0.1% formic acid in MeOH
Time (mins) %B
0 2
4 2
34 100
38 100
40 2
Flow Rate: 0.3 mL/min
Injection: 10 µL
Temperature: 37 °C
Detection: Thermo Quantum Ultra MS
ESI in positive ion mode

Analytes in blue are included in Extracted Ion Chromatogram

4000000

3000000

2000000

1000000

0
0 5 10 15 20 25 30
mins
mins

Reproduced with permission of Toxicology, Clinical Chemistry, Wansbeck General Hospital,

MRM MRM
Analyte Rt Analyte Rt
Transitions Transitions
6-Acetylcodeine 16.9 342.2 > 165.1 Clomethiazole 17.3 161.9 > 113
Alfentanil 21.1 417.4 > 197.2 Clomipramine 27.0 315.1 > 86.1
Alprazolam 23.3 309.11 > 205.1 Clonazepam 23.1 316.1 > 214.2
Aminoclonazepam 13.9 286.2 > 222.3 Clonidine 13.2 230 > 44.2
Aminodesmethylflunitrazepam 20.0 270.1 > 121.1 Clozapine 21.7 327.1 > 192.1
Aminoflunitrazepam 16.2 284.1 > 135.1 Cocaethylene 19.5 318.2 > 82.2
Aminonitrazepam 10.9 252.1 > 94.1 Cocaine 18.0 304.2 > 82.2
Aminorex 12.2 163 > 120.1 Codeine 11.9 300.2 > 153.2
Amiodarone 31.3 646.2 > 100 Cotinine 2.5 177.1 > 80.1
Amisulpride 16.2 370.1 > 242 Creatinine 1.3 114 > 44
Amitriptyline 24.4 278.2 > 91.1 Cyclizine 22.5 267.1 > 167.1
Amlodipine 25.3 409.3 > 238.2 D2PM (Diphenylprolinol) 7.3 254.1 > 130.1
Amlodipine met 22.9 407 > 318 Dehydroaripiprazole 25.3 446.1 > 285.1
Amphetamine 11.6 136.1 > 65.2 Desimipramine 24.3 267.1 > 72.2
Amphetamine-d6 11.6 142.2 > 67.2 N-Desmethylclozapine 21.0 313 > 192.1
Anhydroecgonine methyl ester 9.8 182.2 > 91.1 Desmethylcitalopram 22.4 311.1 > 109.1
Aripiprazole 25.5 448.1 > 285.1 Desmethylflunitrazepam 22.5 300.1 > 254.2
Atenolol 10.7 267.1 >145.1 Desmethylfluoxetine 25.4 296.2 > 134.1
Atomoxetine 23.1 256.2 > 44.1 N-Desmethylmirtazapine 16.1 252.1 > 195.1
Atomoxetine metabolite 22.5 242.2 > 44.1 Desmethylolanzapine 12.6 299.1 > 198.1
Benzedrone 21.7 254 > 65.1 N-Desmethyltramadol 16.7 250.1 > 44.2
Benzoylecgonine 15.6 290.1 > 77.2 O-Desmethyltramadol 12.8 250.1 > 58.2
Benzoylecgonine-d3 15.6 293.2 > 77.2 Desmethylvenlafaxine 15.5 264.3 > 58.1
Benzylpiperazine 5.1 177.2 > 65.1 N-Desmethylzopiclone 20.0 375.1 > 245.1
Bisoprolol 19.9 326.3 > 116.1 Desomorphine 13.1 272.1 > 152.1
Bromazepam 20.4 316 > 182.1 Desoxypipradrol 20.5 252.1 > 91.1
Buphedrone 14.2 178.2 > 160.1 Dextromethorphan 20.0 272.2 > 171.1
Buphedrone ephedrine 13.5 180.4 > 162.2 Diamorphine 16.9 370.1 > 165.1
Bupivacaine 19.5 289.2 > 84.2 Diazepam 25.5 285.1 > 154.1
Buprenorphine glucuronide 18.1 644.4 > 468.4 Didesmethylcitalopram 22.2 297 > 262.1
Bupropion 19.5 240.1 > 131.1 Digoxin 24.2 781.2 > 97
Butylone 14.7 222.1 > 131.1 Dihydrocodeine 11.7 302.2 > 128.1
Caffeine 13.1 195.1 > 110.1 Diltiazem 22.7 415.1 > 178.1
Camfetamine 18.7 202.2 > 67.1 Dimethocaine 16.3 279.3 > 92.1
Carbamazepine 21.5 237.1 > 192.2 Dinitrophenol 18.4 183 > 109
Carbamazepine 10,11-Epoxide 18.6 253.2 > 180.1 Diphenhydramine 21.2 256.1 > 167.1
Cathine 20.0 134.2 > 91.1 Dipipanone 25.0 350.2 > 265.2
Cathinone 9.5 150.2 > 117.1 Donepezil 22.2 380.1 > 91
2C-B-FLY 20.1 286 > 269.1 Dothiepin 23.7 296.2 > 202.2
Chlordiazepoxide 19.5 300.1 > 227.1 Ecgonine ethyl ester 2.5 214.1 > 196.1
Chloroquine 18.5 320.1 > 142.1 Ecgonine methyl ester 1.0 200.1 > 182.1
Chlorpheniramine 20.4 275.1 > 167.2 EDDP 21.4 278.2 > 219.2
Chlorpromazine 25.9 319.2 > 58.1 Estazolam 22.5 295.1 > 267.1
Citalopram 22.2 325.1 > 246.1 Ethylamphetamine 14.5 164.1 > 91.2
Clobazam 22.8 301.1 > 259.2 Ethylmethcathinone 15.8 192.2 > 131.2
Drugs of Abuse Screen (250 Analytes) in
Urine by LC-MS/MS
Application #AN4140
Page 3 of 4

MRM MRM
Analyte Rt Analyte Rt
Transitions Transitions
Ethylphenidate 19.2 248.1 > 56.2 MDAI 12.3 178.19 > 161.1
Etizolam 23.5 343.1 > 314.2 MDEA 15.2 208.1 > 135.1
Fenfluramine 20.3 232.1 > 159.1 MDMA 14.1 194.1 > 135.2
Fentanyl 21.4 337.2 > 105.1 MDPV 18.1 276.1 > 135.1
Flubromazolam 23.2 371.1 > 223.1 MEGX 13.4 207.1 > 58.1
Flunitrazepam 23.5 314.1 > 269.3 MeOPP 13.3 193.2 > 133.1
2-Fluoroamphetamine 13.2 154.1 > 83.1 Mephedrone 14.6 178.1 > 144.2
Fluoroamphetamine interferent 10.6 154 > 67.1 Mescaline 12.7 212.1 > 165.1
3-Fluoromethcathinone 20.0 182.1 > 149.1 Metformin 2.5 130 > 60.1
4-Fluoromethcathinone 20.0 182 > 148.1 Methadone 24.0 310.2 > 105.1
Fluoxetine 25.3 310.1 > 44.2 Methadone-d3 24.0 313.2 > 268.2
Fluphenazine 28.1 438.3 > 143.1 Methamphetamine 12.8 150.1 > 91.2
Flurazepam 21.6 388 > 315 Methaqualone 22.8 251.2 > 91.1
Fluvoxamine 26.0 319.1 > 71 Methedrone 13.9 194.1 > 146.1
Gabapentin 10.5 172.1 > 67.2 Methiopropamine 10.6 156.1 > 97
Glibenclamide 28.2 494.1 > 168.9 Methocarbamol 16.8 242.1 > 118.1
Gliclazide 24.6 324.1 > 110 Methoxetamine 17.2 248.2 > 121.1
Glimepiride 28.4 491.1 > 126 3-Methoxytyramine 6.6 168 > 91
Glipizide 24.5 446.1 > 286 Methylethcathinone 20.0 192.1 > 144.2
Haloperidol 23.1 376.1 > 95.1 Methylhexanamine 13.4 116.1 > 57.3
Hippuric acid 10.9 180 > 77 Methylone 12.7 208.1 > 132.1
Hydrocodone 13.1 300.1 > 199.1 Methylphenidate 16.5 234.1 > 56.2
Hydromorphone 10.4 286.2 > 185.1 5-Methyltryptamine 16.9 175.1 > 143
Hydroxyalprazolam 22.4 325.1 > 216.1 Metoclopramide 17.2 300.1 > 227.1
4-Hydroxyamphetamine 5.4 152.1 > 107.1 Midazolam 20.9 326.1 > 249.1
Hydroxybupropion 18.5 253.1 > 130.1 Mirtazapine 16.6 266.1 > 72.2
4-Hydroxymethamphetamine 20.0 166.1 > 107.1 Mitragynine 22.6 399.3 > 174.1
8-Hydroxymirtazapine 15.6 282.1 > 211 Modafinil 21.1 296.1 > 129
7-Hydroxymitragynine 18.2 415.3 > 175.1 6-Monoacetylmorphine 12.9 328.1 > 165.1
3-Hydroxyphenazepam 23.3 366.9 > 320.8 Mono-N-desethylamiodarone 30.8 618.2 > 547.2
7-Hydroxyquetiapine 15.3 400.3 > 208.1 Morphine 7.7 286.1 > 152.2
9-Hydroxyrisperidone 19.5 427.2 > 69.1 Morphine glucuronide 2.7 462.2 > 201.1
Imipramine 24.2 281.1 > 86.2 Morphine-d3 7.7 289.2 > 152.2
5-Iodo-2-aminoindane 18.4 260.1 > 115.1 Naloxone 20.0 328.3 > 212.1
Ketamine 15.4 238.1 > 125.1 Naphyrone 23.2 282.2 > 141.1
Lamotrigine 16.8 256.1 > 211.1 Nefopam 19.5 254.9 > 166.1
Levamisole 13.3 205.1 > 91.1 Nifoxipam 20.9 316.05 > 298.1
Levetiracetam 8.9 171.2 > 126.1 Nimetazepam 23.6 296.1 > 250.2
Lidocaine 14.8 235.1 > 86.2 Nitrazepam 22.8 282.1 > 236.1
Lorazepam 22.8 321 > 229.1 Noralfentanil/sufentanil 19.4 277.1 > 245.1
Lormetazepam 23.8 335 > 289.1 Norbuprenorphine glucuronide 15.5 590.3 > 414.3
LSD 20.1 324.3 > 223.1 Norcyclizine 21.9 253.2 > 167.1
MCAT 5.4 164.2 > 130.1 Nordiazepam 24.4 271.1 > 140.1
mCPP 17.0 197.1 > 118.1 Nordothiepin 24.4 282.1 > 202.1
MDA 13.4 180.1 > 133.1 Norfentanyl 15.6 233.2 > 56.2
Drugs of Abuse Screen (250 Analytes) in
Urine by LC-MS/MS
Application #AN4140
Page 4 of 4

MRM MRM
Analyte Rt Analyte Rt
Transitions Transitions
Norketamine 14.8 224.1 > 125.1 Propoxyphene 23.6 340.2 > 58.2
Normorphine 3.8 272.1 > 165.2 Propranolol 23.0 260.1 > 157.1
Nornefopam 19.7 240.9 > 166.1 Quetiapine 21.7 384.1 > 221.1
Noroxycodone 13.0 302.1 > 284.1 Remifentanil 18.4 377.3 > 113.1
Norpropoxyphene 23.0 308 > 44.2 Risperidone 20.4 411.1 > 190.8
Norsertraline 20.0 275.3 > 159 Ritalinic acid 14.4 220.2 > 56.1
Nortriptyline 24.6 264.1 > 91.1 Sertraline 25.6 306.1 > 159
Olanzapine 13.4 313.1 > 84.1 Sildenafil 23.6 475.4 > 58.1
Orphenadrine 23.4 270.1 > 181.1 Sildenafil N-oxide 23.9 491.4 > 312.3
Oxazepam 23.5 287.1 > 104.1 Sufentanil 22.9 387.3 > 140.2
Oxybutynin 24.6 358.1 > 141.9 Temazepam 24.2 301.1 > 177.1
Oxycodone 12.9 316.1 > 241.1 Temazepam-d5 24.1 306.1 > 260.2
Oxymorphone 20.0 302 > 227 Tetrazepam 24.5 289.2 > 225.2
Paracetamol 7.8 152.1 > 65.1 TFMPP 7.9 231.1 > 118.1
PCP 20.5 244.3 > 86.2 Theophylline 11.2 181.1 > 124.1
Pentazocine 19.5 286.3 > 175.2 Tramadol 16.9 264.2 > 58.2
Pentedrone 16.0 192.2 > 131.1 Trazodone 23.9 372.2 > 179.2
Phenazepam 24.5 350.9 > 206 Trifluoperazine 28.8 408.2 > 113.2
Pheniramine 15.1 241.2 > 196.2 Trihexyphenidyl 23.7 302.1 > 70.1
Phenytoin 20.0 253.1 > 77 Varenicline 12.3 212.2 > 168.1
Pholcodine 9.2 399.2 > 114.1 Venlafaxine 19.1 278.2 > 58.2
PMA 13.8 149.2 > 91.1 Verapamil 23.9 455.2 > 150.1
PMMA 14.6 180.2 > 121.1 Vigabatrin 2.5 130.1 > 71.1
Powder 20140730 18.7 248.3 > 84.2 Warfarin 25.7 309.1 > 251.1
Prazepam 27.0 325 > 140 Zaleplon 21.5 306.1 > 236.2
Pregabalin 10.4 160.1 > 97.2 Zolpidem 18.8 308.2 > 235.2
Procyclidine 22.4 288.3 > 42 Zolpidem phenyl COOH 15.3 338 > 265.1
Promethazine 20.0 285.1 > 86.2 Zopiclone 16.5 389.1 > 217.1
Propofol 23.9 179 > 137 Zopiclone N-oxide 17.6 405.2 > 217.1
Propofol glucuronide 20.1 372.2 > 148.1