Abnormalities in cell growth

Fixative according to composition Alcoholic Fixatives MINCE

“Pangulong Rodrigo Duterte”  AMPAO Methyl alcohol (100%)
 Progressive (larger than normal) Aldehyde, Metallic, Picric, Alcohol-based, Osmium Isopropyl alcohol (95%)
tetroxide Newcomers fluid
 Retrogressive (smaller than normal)
Carnoys fluid
 Degenerative (cellular growth)
Fixative according to action Ethyl alcohol (70-100%)
Secondary changes (signs of death)  MiNCH
Microanatomical, Nuclear, Cytoplasm, Histochemical Osmium tetroxide
ARaL Pa Dasal PA Flemmings
A- Algor mortis Aldehyde
R- Rigor mortis 10% Formaldehyde MICROANATOMICAL FIXATIVE
L- livor mortis 10% NBF 10% BBZZ FOR HEIDEN
P- post mortem clot FORMOL CORR -10% formol saline
D-dessication ALCOHOLIC FORMALIN (gendre) -10% neural buffered formalin
P-putrefaction  Gluteraldehyde -bouins
A-autolysis -brasil
Metallic Fixative -formol sublimate
-heidenhains susa
Honing – Heal to toe ; Nicks
Mercuric chloride BHZZ
StroPPing- toe to Heal; buRRs
B5 fixative NUCLEAR FIXATIVE
Heindeinhains Susa BFNCH
Autotechnicon
Zenker -Bouins
F FIXATION (1-2) 10% formalin
Zenkers formol (hellys) -Flemmings
D DEHYDRATION (3-6) 70-95%
-Newcomers
Acetone (7-8) Further fd
C CLEARING (9-10) Chloro’ xylol Chromate CROP -Carynoys
I INFILTRATION (11-12) Liq. paraffin Chromic acid -Heidenhains
Regauds solution
Most commonly used method for freezing Orths solution CYTOPLASMIC FIXATIVE
LICA Potassium dichromate HROFF
-L iquid nitrogen Leads fixative -Helly
-I sopentane cooled by liquid nitrogen Picric acid BB -Regauds
-C arbon dioxide gas Bouins -Orth
-A erosol sprays Brasils -flemming without GAC
-formalin with post chroming
HISTOCHEMICAL FIXATIVE 90% Ethanol saturated with picric acid -Lymph nodes
10% FANA 40% Formalin
10% formol saline Composition of Carnoys
Absolute Ethyl Alcohol Mercuric Chloride Stock Solution GAC
Newcomers MaPle Syrup Disease -glacial acetic acid
Acetone -Mercuric chloride -alcohol
Lipid fixation – 3F’S -Potassium dichromate -chloroform
 FAT -Sodium sulfate
 FROZEN SECTION -Distilled water
 FORMALIN Rabies fixative: CAR
Zenker Formol (Helly's) used: -Carnoys
Glycogen fixative: GAP PBB -Acetone
-Alcohol -Pituitary
-Picric acid -Blood Dehydrating agents:
-Bone marrow ACED TITE
Electron Microscopy fixatives Acetone
GOPKZ Regaud’s (Muller’s) used: Cellosolve
Gluteraldehyde Mabait sila so perfect nila ang GMRC Ethyl alcohol
Osmium tetroxide -Golgi bodies Dioxane
Paraformaldehyde -Mitochondria Triethyl phosphate
Karnovsky -RBC Tetrahydrofuran
Zambonis -Colloid containing tissue
Clearing
10% Neutral buffered formalin composition Bouin’s – B for baby (embryo) CCC
40% si SARA DD Composition: FOR GLAm Pic Cedarwood oil
-Formalin CNS
40% formaldehyde -Glacial acetic acid Cytological studies
Sodium dihydrogen phosphate -saturated solution of Picric acid
Disodium hydrogen phosphate Dry Celloidin Method (EMBEDDING AND IMPREGNATION)
Carnoy’s Fixative used: GCC
Distilled water
CURL ang buhok ni Carnoy Gilson mixture
-Chromosomes Chloroform
Alcoholic formalin
-Urgent biopsies Cedar wood oil
GEndre’s Formalin
-Rabies
Glacial acetic acid
DOUBLE EMBEDDING METHOD
ICEP Hematoxylin is originally red, it is converted to its blue- Stains for Collagen
blue black color when adding blueing agents VgMMAK
Infiltration using Celloidin
Mnemonics for BLUING AGENTS Van gieson
Embedding using Paraffin “kailangan mo ng TSAAS (teas?) para mag blue” Masson’s trichrome- BLUE
-Tap water Methyl violet-crystal violed
Mga sumasabog sa Histopath
-Saturated lithium carbonate Kajian’s technique (congo red)
Lagot FOR Da Putok
Low viscosity Nitrocellulose -0.5% Ammonia in distilled water
Stains for Elastic fiber
Formalin -Ammonium hydroxide
VOW
Dioxane -Scotts solution
Verhoeff’s
Picric Acid
Note that Hematoxylin is NOT a true basic dye it is needed Orcein
ELECTRON MICROSCOPY MNEMONICS to convert to HEMATEIN which is the active coloring agent Weigert’s elastic tissue
to convert we have the process called RIPENING which is
Si GOPKZ na PLASTIC at EPAl sa BGC nainlove kay PaUL an oxidation process. Aqueous mounting media
Embedding method for EM: PLASTIC WAG FB
Classification of plastic embedding Artificial Ripening : Water
Epoxy SHIMP Apathy’s
B-BISPHENOL A Sodium iodate Glycerin jelly
G-GLYCEROL Farrant’s medium
Hydrogen Peroxide
C-CYCLOHEXENE DIOXIDE Brun’s fluid
Polyester plastic
Iodine
Acrylic plastics Mecuric chloride Stains for Papsmear
Potassium permanganate  Harris hematoxylin
Stains for Electron Microscopy  EA 50
PaUL Types of Hematoxylin  OG 6
Phospotungstic acid ATIC
Orange G – “OG” original-matanda—MATURE CELLS are
Uranyl acetate Aluminum hematoxylin
stained
Lead Tungsten hematoxylin EA 50 – EMMATURE cells are stained
Iron hematoxylin
Copper hematoxylin Response of Vaginal Epithelium to steroid Hormones:
NOTE: BOTH OF THESE HORMONES INCREASES THE CELLS Alcohol
ESPI Chloroform
Estrogen increases Superficial cells Acetic acid
Progesterone increases Intermediate cells
Von ebner’s = aNAHaW
Mnemonics for Autopsy Sodium chloride NA
Hydrochloric acid Hcl
Technique of Virchow
VirchONE Water
-organs removed ONE by ONE

Technique of Gohn
Gohn= 4 letters
BLOC=4 letters
“En bloc”

Technique of M. letulle
leTTule
en maSSe

additional mnemonics for HP:

PIC FOR ACE si BOUIN’S
-picric acid
-formalin
-acetic acid

Anong sabi ni HELLY na asawa ni Mercury?

Die FOR MERCUry

Carnoys – ACA