User’s Manual

FreeZone® 1 Liter Benchtop

Freeze Dry Systems
Models
7740020
7740021
7740030
7740031
7740040
7740041

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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com

Please read the User’s Manual before operating the equipment.

Copyright © 2004, 2007 Labconco Corporation. All rights reserved.

The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.

Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas
of defective material and workmanship, provided such defect results from normal and proper use of
the equipment.

The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
• Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Lab Animal Research
Stations carry a three-year warranty from date of installation or four years from date of
shipment from Labconco, whichever is sooner.
• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from
date of installation or three years from date of shipment from Labconco, whichever is
sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair
or replace all defective parts to restore the unit to operation. Under no circumstances shall
Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This
statement may be altered by a specific published amendment. No individual has authorization to
alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by
this warranty. Damage due to corrosion or accidental breakage is not covered.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.

Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.

Part #7429300, Rev. C

ECO E139
TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION 1
Freeze Dry Process 1
Freeze Dry Rates 2
Freeze Dry Capacity 3
Samples Containing Volatile Substances 4
About This Manual 4
Typographical Conventions 6
Your Next Step 6

CHAPTER 2: PREREQUISITES 7
Electrical Requirements 7
Location Requirements 8
Vacuum Pump Requirements 8
Chamber or Manifold Requirements 8

CHAPTER 3: GETTING STARTED 9

Unpacking Your Freeze Dryer 9
Freeze Dryer Components 10
Setting Up Your Freeze Dryer 11
Vacuum Pump Connection 11
Electrical Connection 11
Drying Chamber or Drying Manifold Installation 12
Chemical Resistance of Freeze Dryer Components 12
Solvent Safety Precautions 13

CHAPTER 4: USING YOUR FREEZE DRYER 15

Freeze Dryer Controls 16
Operation Checklist 16
Vacuum Pump Ballast Setting 17
Operating the Freeze Dryer 17
Set-Up 17
Automatic Start-Up 19
Manual Start-Up 20
Pre Freezing Samples 21
Adding Sample 21
Shut Down 22
 Defrosting 22

CHAPTER 5: MAINTAINING YOUR FREEZE DRYER 24

CHAPTER 6: TROUBLESHOOTING 26
Vacuum Pump 26
Gaskets, Tubing, Connections, Sample Valves 27
System Components & Collection Chamber Isolation 29
Refrigeration Module Operation 31

CHAPTER 7: MODIFYING YOUR FREEZE DRYER DISPLAY 32

Temperature and Vacuum Display 32
Modifying the Display 32

APPENDIX A: FREEZE DRYER COMPONENTS 34

APPENDIX B: FREEZE DRYER DIMENSIONS 37

APPENDIX C: FREEZE DRYER SPECIFICATIONS 38

Electrical Specifications 38
Environmental Conditions 38
Wiring Diagram (115V) 39
Wiring Diagram (230V) 40

APPENDIX D: FREEZE DRYER ACCESSORIES 41

DECLARATION OF CONFORMITY 49
 CHAPTER 1
INTRODUCTION
Congratulations on your purchase of a Labconco FreeZone®
Freeze Dry System, which is designed for laboratory lyophilization
procedures. The refrigerant used in the refrigeration system is
CFC-free so it will not endanger the environment. The unit is easy
to install and maintain. Proper care and maintenance of this
product will result in many years of dependable service.

Freeze Dry Process

Freeze drying is an important process in sample preparation and
for the preservation and storage of biologicals, pharmaceuticals
and foods. Of the various methods of dehydration, freeze drying
(lyophilization) is especially suited for substances that are heat
sensitive. Other than food processing (e.g., coffee, whole dinners),
freeze drying has been extensively used in the development of
pharmaceuticals (e.g., antibiotics) and preservation of biologicals
(e.g., proteins, plasma, viruses and cell lines). The nondestructive
nature of this process has been demonstrated by the retention of
viability in freeze dried viruses and microorganisms.

Freeze drying is a process whereby water or other solvent is

removed from frozen material by converting the frozen water
directly into vapor without the intermediate formation of liquid
water. The basis for this sublimation process involves the
absorption of heat by the frozen sample in order to vaporize the
ice; the use of a vacuum pump to enhance the removal of water
vapor from the surface of the sample; the transfer of water vapor to
a collector; and the removal of heat by the collector in order to
condense the water vapor. In essence, the freeze dry process is a
balance between the heat absorbed by the sample to vaporize the
ice and the heat removed from the collector to convert the water
vapor into ice.

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Chapter 1: Introduction

Freeze Dry Rates

The efficiency of the freeze drying process is dependent upon the
surface area and the thickness of the sample, the collector
temperature and vacuum obtained, the eutectic point and solute
concentration of the sample. It is important to remember these
factors when trying to obtain efficient utilization of your freeze dry
system. A listing of selected materials and their approximate
drying times are shown in Table 1 for your reference.

Table 1
SAFE TEMPERATURE AND DRYING TIMES
FOR SELECTED MATERIALS
Material Safe Collector Hours
10mm Thick Temperature Temperature (Approx.)
°C °C
Milk -5 -40 10
Urea -7 -40 10
Blood Plasma -10 to -25 -40 16
Serum -25 -40 18
Vaccinia -30 to -40 -50 22
Influenza Vaccine -30 -50 24
Human Tissue -30 to -40 -50 48
Vegetable Tissue -50 -80 60
*Total sample quantities are contingent on various freeze dryer capacities.

Up to the point of overloading the system, the greater the surface

area of the sample, the faster the rate of freeze drying. By contrast,
for a given surface area, the thicker the sample the slower the rate
of freeze drying. This is based on the fact that the heat of
sublimation is usually absorbed on one side of the frozen sample
and must travel through the frozen layer to vaporize water at the
other surface. In addition, as the sample is freeze dried, the water
vapor must travel through the layer of dried material. The thicker
the sample, the greater the chance that the dried layer may collapse
which would cause an additional decrease in the rate of freeze
drying.

The surface area and thickness of the sample can usually be

ignored when each sample contains only a few milliliters.
However, for larger volumes, the samples should be shell frozen to
maximize the surface area and minimize the thickness of the
sample. The volume of the freeze dry flask should be two to three
times the volume of the sample.

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 Chapter 1: Introduction

In order for lyophilization to occur, ice must be removed from the

frozen sample via sublimation. This is accomplished by the
collector and the vacuum pump. The collector, which should be at
least 15 to 20°C colder than the eutectic temperature (melting
temperature) of the sample, traps vapor as ice. Since the vapor
pressure at the collector is lower than that of the sample, the flow
of water vapor is from the sample to the collector. Since this vapor
diffusion process occurs very slowly under normal atmospheric
conditions, a good vacuum is essential to maintain an efficient rate.
In many applications, the maintenance of a vacuum of 0.133 mBar
or less is recommended.

The rate of freeze drying is directly proportional to the vapor

pressure and the vapor pressure is dependent upon both eutectic
temperature and solute concentration of the sample. For example,
a solution of sodium chloride and water would freeze dry at a
slower rate than pure water. The eutectic temperature of a sodium
chloride solution is about –21°C and at this temperature the vapor
pressure is about 1/16 that of water at 0°C. Although the eutectic
temperature is not dependent upon the concentration of sodium
chloride, the vapor pressure of the water would decrease as the
concentration of sodium chloride increased. This is due to the fact
that as the solute concentration increases, less of the surface area of
the frozen sample is occupied by water. In general, most solutions
or biological samples will have a eutectic temperature of –10 to
–25°C. However, if the sample contains a simple sugar such as
glucose or if the sample is animal or plant tissue, the eutectic
temperature may be as low as –30° to –50°C.

Freeze Dry Capacity

The volume of a sample that can be freeze dried at one time is
related to factors discussed previously and the size and design of
the freeze dry system. With any given instrument, the capacity is
based on the surface area of the sample; the eutectic temperature
and concentration of the sample; and the rate and amount of heat
transferred to the frozen sample. Of these factors, the eutectic
temperature is the most important factor in determining the amount
of sample that can be freeze dried at one time, particularly when
flasks are used. This is because as the eutectic temperature
decreases, the vapor pressure decreases but the rate of heat
absorption by the sample does not change. This tends to promote
melting of the sample which leads to a marked increase in vapor
pressure and ultimately overloads the collector and vacuum pump.
Samples that have eutectic temperatures of –20°C or lower should
be placed on the freeze dry system one flask at a time so that the

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Chapter 1: Introduction

vacuum in the system may recover before adding another sample

to the system. If the vacuum does not recover, the capacity of the
freeze dry system has been exceeded and the sample should be
removed.

If there is a problem with a particular type of sample melting when

placed on the freeze dry system, dilution of the sample with more
water or providing some insulation around the flask to decrease the
rate of heat absorption by the sample may help. If the eutectic
temperature of the sample is –40 to –60°C, the freeze dry system
selected for use must be equipped with cascade type refrigeration
so that the collector temperature can be cooled to below –75°C, or
a dry ice/solvent trap may be used between the collector and the
vacuum pump.

Samples Containing Volatile

Substances
In certain cases the solvent in a sample to be freeze dried may
contain volatile components such as acetonitrile, methanol, acetic
acid, formic acid or pyridine. In addition to these substances
having an effect on the eutectic temperature, they may increase the
vapor pressure at the surface of the sample. Also, compared to
water, they will require the absorption of less heat for sublimation
to occur. Hence, samples that contain volatile substances will have
a greater tendency to melt, particularly when placed in flasks or
exposed to room temperature. If a sample containing a volatile
substance tends to melt when placed on a freeze dry system,
dilution of the sample with more water will help keep the sample
frozen. For example, a 0.2M solution of acetic acid is much easier
to freeze dry than a 0.5M solution.

About This Manual

This manual is designed to help you learn how to install, use, and
maintain your Freeze Dryer. Instructions for performing routine
maintenance and making minor modifications to your Freeze Dryer
are also included.

Chapter 1: Introduction provides a brief overview of the Freeze

Dryer, explains the organization of the manual, and defines the
typographical conventions used in the manual.

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 Chapter 1: Introduction

Chapter 2: Prerequisites explains what you need to do to prepare

your site before you install your Freeze Dryer. Electrical
requirements are discussed.

Chapter 3: Getting Started contains the information you need to

properly unpack, inspect and install your Freeze Dryer.

Chapter 4: Using Your Freeze Dryer discusses the basic operation

of your Freeze Dryer. Information on how to attach samples and
run the Freeze Dryer is included.

Chapter 5: Maintaining Your Freeze Dryer explains how to

perform routine maintenance on your Freeze Dryer.

Chapter 6: Troubleshooting contains a table of problems you may

encounter while using your Freeze Dryer, including the probable
causes of the problems, and suggested corrective actions.

Chapter 7: Modifying your Freeze Dryer Display explains how to

recalibrate your Freeze Dryer display.

Appendix A: Freeze Dryer Components contains labeled diagrams

of the components of the Freeze Dryer.

Appendix B: Freeze Dryer Dimensions contains comprehensive

diagrams showing the dimensions for the Freeze Dryer.

Appendix C: Freeze Dryer Specifications contains product

specifications. A wiring diagram for the Freeze Dryer is also
included.

Appendix D: Freeze Dryer Accessories lists the part numbers and

descriptions of all of the accessories available for your Freeze
Dryer.

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Chapter 1: Introduction

Typographical Conventions
Recognizing the following typographical conventions will help
you understand and use this manual:

• Book, chapter, and section titles are shown in italic type (e.g.,
Chapter 3: Getting Started).
• Steps required to perform a task are presented in a numbered
format.
• Comments located in the margins provide suggestions,
reminders, and references.
• Critical information is presented in boldface type in paragraphs
! that are preceded by the exclamation icon. Failure to comply
with the information following an exclamation icon may result
in injury to the user or permanent damage to your Freeze
Dryer.
• Important information is presented in capitalized type in
) paragraphs that are preceded by the pointer icon. It is
imperative that the information contained in these paragraphs
be thoroughly read and understood by the user.

Your Next Step

If your Freeze Dryer needs to be installed, proceed to Chapter 2:
Prerequisites to ensure your installation site meets all of the
requirements. Then, go to Chapter 3: Getting Started for
instructions on how to install your Freeze Dryer and make all of
the necessary connections.

For information on the operational characteristics of your Freeze

Dryer, go to Chapter 4: Using Your Freeze Dryer.

If your Freeze Dryer is installed and you need to perform routine

maintenance on the Freeze Dryer, proceed to Chapter 5:
Maintaining Your Freeze Dryer.

Refer to Chapter 6: Troubleshooting if you are experiencing

problems with your Freeze Dryer.

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 CHAPTER 2
PREREQUISITES
Before you install your Freeze Dryer, you need to prepare your site
for installation. Carefully examine the location where you intend
to install your Freeze Dryer. You must be certain that the area is
level and of solid construction. An electrical source must be
located near the installation site.

Carefully read this chapter to learn:

• the electrical supply requirements.

• the vacuum pump requirements.

Refer to Appendix C: Freeze Dryer Specifications for complete

Freeze Dryer electrical and environmental conditions,
specifications and requirements.

Electrical Requirements
The Freeze Dryer requires a dedicated electrical outlet. This outlet
requires a 20 Amp circuit breaker or fuse for models rated at 115V
(60 Hz). An outlet equipped with a 15 Amp circuit breaker or fuse
is required for models rated at 230V (50/60 Hz). The power cord
on 115V models are equipped with a 20 Amp NEMA 5-20P plug.
The power cord on the 230V models is equipped with a NEMA 6-
20P plug. If this does not match the available receptacle, remove
this plug and replace it with an approved plug of the suitable style.

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 Chapter 2: Prerequisites

Location Requirements
The Freeze Dryer should be located in an area that provides an
unobstructed flow of air around the cabinet. This air cools the
refrigeration system. The refrigeration system draws air through
the rear panel and exhausts it through the sides. A minimum of 3"
must be allowed between the back and both sides of the Freeze
Dryer and adjacent wall surfaces. Restriction of airflow during
operation could adversely affect performance.

Refer to Appendix C: Freeze Dryer Specifications for dimensional

drawings of the Freeze Dryer. The space must be large enough to
accommodate the vacuum pump, which will be used with the
Freeze Dryer.

Vacuum Pump Requirements

A vacuum pump must be provided by the user. A vacuum pump
with a displacement of 86 liters per minute and 0.0002 mBar
ultimate pressure is adequate for most samples. The inlet fitting on
the vacuum pump must be suitable for 3/4" ID vacuum hose,
which is provided. It is recommended that the vacuum pump is
equipped with an exhaust filter to minimize oil mist exhausting
from the vacuum pump.

Vacuum pumps used with 115V models should be equipped with a

115V, 15 Amp NEMA 5-15P plug. Vacuum pumps used with
230V models should be equipped with a reverse IEC plug. This
plug is included with 230V models. This will allow the vacuum
pump to be plugged into the receptacle on the back panel of the
freeze dryer. See Appendix D: Freeze Dryer Accessories for
vacuum pumps available from Labconco.

Chamber or Manifold
Requirements
A freeze drying chamber or manifold is not included and must be
purchased separately. These allow samples to be attached to the
Freeze Dryer. See Appendix D: Freeze Dryer Accessories for
available chambers and manifolds.

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 CHAPTER 3
GETTING STARTED
Now that the site for your Freeze Dryer is properly prepared, you
are ready to unpack, inspect, install and test your Freeze Dryer.
Read this chapter to learn how to:

• unpack and move your Freeze Dryer.

• set up your Freeze Dryer.
• connect the electrical supply source to your Freeze Dryer.
• properly exhaust your Freeze Dryer.
• safely use solvents with your Freeze Dryer.

The FreeZone 1 Liter Freeze Dry System weighs

! over 82 lbs. (37 Kg). If you must lift the Freeze
Dryer manually, use at least two (2) persons and
follow safe lifting guidelines.

Unpacking Your Freeze Dryer

Carefully unpack your Freeze Dryer and inspect it for damage that
The United States
may have occurred in transit. If your Freeze Dryer is damaged, Interstate Commerce
notify the delivery carrier immediately and retain the entire Commission rules
shipment intact for inspection by the carrier. require that claims be
filed with the delivery
carrier within fifteen (15)
DO NOT RETURN GOODS WITHOUT THE

)
days of delivery.
PRIOR AUTHORIZATION OF LABCONCO.
UNAUTHORIZED RETURNS WILL NOT BE
ACCEPTED.

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Chapter 3: Getting Started

IF YOUR FREEZE DRYER WAS DAMAGED IN

) TRANSIT, YOU MUST FILE A CLAIM

DIRECTLY WITH THE FREIGHT CARRIER.
LABCONCO CORPORATION AND ITS
DEALERS ARE NOT RESPONSIBLE FOR
SHIPPING DAMAGE.

DO NOT DISCARD THE CARTON OR

) PACKING MATERIAL FOR YOUR FREEZE

DRYER UNTIL YOU HAVE CHECKED ALL OF
THE COMPONENTS AND INSTALLED AND
TESTED THE FREEZE DRYER.

Freeze Dryer Components

Locate the model of Freeze Dryer you received in the following
table. Verify that the components listed are present and
undamaged.

Catalog # Product Description

7740020 1 Liter Freeze Dryer – 115V, 60 Hz
7740021 1 Liter Freeze Dryer with Teflon-Coated Chamber – 115V, 60 Hz
7740030 1 Liter Freeze Dryer – 220/240V, 50 Hz
7740031 1 Liter Freeze Dryer with Teflon-Coated Chamber – 220/240V, 50 Hz
7740040 1 Liter Freeze Dryer – 208/230V, 60 Hz
7740041 1 Liter Freeze Dryer with Teflon-Coated Chamber – 208/230V, 60 Hz
Plus the Following:
Part # Component Description
7429300 User’s Manual
1336400 Power Cord – 115V
or
1342100 Power Cord – 230V
1291000 Plug (230V models only)
7646000 Vacuum Hose
1967600 Hose Clamps (2)
7690000 Chamber Lid
7688500 Lid Gasket

If you did not receive one or more of the components listed for
your Freeze Dryer, or if any of the components are damaged,
contact Labconco Corporation immediately for further instructions.

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 Chapter 3: Getting Started

Setting Up Your Freeze Dryer

After you verify receipt of the proper components, move your
Freeze Dryer to the location where you want to install it. Then,
follow the steps listed below.

Vacuum Pump Connection

A vacuum pump as described in Chapter 2: Prerequisites is
required to operate your Freeze Dry System properly. The Freeze
Dryer is equipped with a 3/4" ID, heavy wall, vacuum hose for
connecting to the collector chamber to the vacuum pump.

Make certain that the oil in the vacuum pump is

! at the proper level.

Place the vacuum pump near the Freeze Dryer. Connect the
vacuum pump power cord to the receptacle on the back of the
cabinet labeled “vacuum pump”. If the vacuum pump has an
off/on switch, turn the switch on. The vacuum pump will be
controlled by the Freeze Dryer. Connect the vacuum pump inlet
port to the vacuum tube on the back of the cabinet with the vacuum
hose and clamps provided. If necessary, cut the hose to proper
length to allow for gentle bends without kinks.

Models rated for use on 230V have a reverse IEC connector for
attaching the vacuum pump power cord. A plug is supplied that
can be installed on the vacuum pump power cord, if necessary.

Electrical Connection
Plug the power cord into the receptacle on the back of the Freeze
Dryer and plug the other end into a suitable power receptacle.

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Chapter 3: Getting Started

Drying Chamber or Drying Manifold

Installation
The drying chamber or drying manifold, purchased separately, may
be positioned directly above the 3-inch hole in the collector
chamber lid. When vacuum is applied to the system, the chamber
will be held securely in place.

Chemical Resistance of Freeze

Dryer Components
The FreeZone Freeze Dry System is designed to be chemically
resistant to most compounds that are commonly used in freeze
drying processes. However, by necessity, the freeze dryer is
comprised of a number of different materials, some of which may
be attacked and degraded by certain chemicals. The degree of
degradation is dependent on the concentration and exposure
duration. Some of the major components of the FreeZone Freeze
Dry System that are susceptible to degradation are as follows:

Acids Buffers Solvents

Methyl t-Butyl Ether (BTBE)

Trifluoroacetic Acid (TFA)

Carbon Tetrochloride
Sodium Phosphate
Calcium Chloride
Acetic Acid 20%

Cyclohexane
Acetonittirle
Formic Acid

Pyridine
Dioxane
Acetone

Component Material
Valve Stem Acetal C D D D D
(Delrin)
Collector Acrylic D D D D
Lid
Hoses, Neoprene C D D C C D D D C D
Gaskets &
Valve
Bodies
Flask Top Silicon C D D D D D C D
Rubber
Chamber & Stainless C
Fittings Steel
C – Moderate degradation; Limited use
D – Severe degradation; Infrequent use recommended; Immediate
thorough cleaning required.

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 Chapter 3: Getting Started

• Most common compounds used in freeze drying processes, if

allowed to enter the vacuum pump, will degrade the oil and
cause damage to the vacuum pump.
• Sugars and proteins typically will have minimal negative effect
on any of the materials of construction.

When using compounds in the Freeze Dryer that are hostile to the
materials of construction, it is imperative that the equipment is
thoroughly cleaned after use.

• Rubber and plastic components that have been exposed to

damaging compounds should be removed and flushed with
water.
• The oil in the vacuum pump should be checked often. It must
be changed if it is cloudy, shows particles or is discolored. The
useful life of vacuum pump oil can be extended if the vacuum
pump is operated for an extended period of time after a freeze
dry run. This allows contaminants to be purged from the hot
oil. This must be done with the inlet to the pump blocked off
to prevent air from free flowing through the pump. If the pump
is operated at an elevated vacuum level, (>10mBar) oil may be
expelled from the pump and damage could occur.

Another way to extend the life of the vacuum pump is to install an

optional secondary trap in the line between the Freeze Dryer and
the vacuum pump. Contact Labconco for ordering information.

With prudent maintenance the FreeZone Freeze Dry System will

provide years of service. Warranty on the affected parts will be
voided if maintenance has been obviously neglected. If you have
questions about using specific compounds in the Freeze Dryer,
contact Labconco Technical Service at 1-800-821-5525 or 816-
333-8811 or e-mail: labconco@labconco.com.

Solvent Safety
! Precautions
Solvents used in the Freeze Dryer may be
flammable or hazardous to your health. Use
extreme caution and keep sources of ignition
away from the solvents.

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Chapter 3: Getting Started

When using flammable or hazardous solvents,

the vacuum pump must be vented to or operated
inside a fume hood.

Hazardous materials such as strong acids or

bases, radioactive substances and volatile
organics must be handled carefully and
promptly cleaned up if spilled. If a sample is
spilled in the collector chamber it must
immediately be cleaned up.

WARNING: The disposal of substances used in

connection with this equipment may be governed
by various Federal, State or local regulations.
All users of this equipment are urged to become
familiar with any regulations that apply in the
user’s area concerning the dumping of waste
materials in or upon water, land or air and to
comply with such regulations.

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 CHAPTER 4
USING YOUR FREEZE
DRYER
After your Freeze Dryer has been installed as detailed in Chapter
3: Getting Started, you are ready to begin using your Freeze Dryer.
Read this chapter to learn how to:

• operate the controls.

• understand the display.
• connect samples.

Do not use the Freeze Dryer in a manner not

! specified by the manufacturer (refer to Appendix
C: Freeze Dryer Specifications). The electrical
protection properties of the Freeze Dryer may be
impaired if the Freeze Dryer is used
inappropriately.

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Chapter 4: Using Your Freeze Dryer

Freeze Dryer Controls

The control panel for the Freeze Dryer is shown below with a
description about its function.

2
4

3
1. LCD Display – Displays system operating parameters and
set-up parameters.
2. Menu Switch – This switch is used to change the display from
operating system parameters to set-up parameters.
3. Select Switch – Used to select set-up parameters.
4. Vacuum Switch – Used to start the vacuum pump manually or to stop
the vacuum pump when operating in either the auto or manual start-up
mode.
5. Main Power Switch – Turns the Freeze Dryer on or off. (Not
shown, located on the right side of the cabinet).

Operation Checklist
The following checklist should be followed prior to each use of
your Freeze Dryer:
1. Wipe the interior of the collector chamber with a soft cloth or
paper towel to remove any accumulated moisture.
2. Check the collector chamber drain hose to ensure that the hose
is free of moisture and that the drain plug is securely installed.
3. Using a soft, lint-free cloth or paper towel, wipe the collector
chamber lid gasket to remove any dirt and contaminants that
could cause a vacuum leak. Vacuum grease is not required on
the lid gasket to obtain a proper vacuum seal.

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 Chapter 4: Using Your Freeze Dryer

4. Remove the accessory drying chamber or manifold from the

connection port and using a soft, lint-free cloth or paper towel,
wipe the port gasket and sealing surfaces of the drying
chamber/manifold to remove any dirt and contaminants that
could cause a vacuum leak. Reinstall the drying chamber or
manifold on the port. Vacuum grease is not required on the
port gasket to obtain a proper vacuum seal.
5. Check that each sample valve is closed or in the “vent”
position.

Vacuum Pump Ballast Setting

Most vacuum pumps are equipped with a gas ballast mechanism.
The freeze dry process requires high vacuum (0.133 mBar).
Therefore, it is recommended that the gas ballast be closed during
the operation of the Freeze Dry System. If the gas ballast is left
open for extended periods of operation, the oil can be pumped out
the exhaust, causing the pump to fail.

Operating the Freeze Dryer

Set-Up
The Freeze Dryer may be configured to automatically start the
vacuum pump when the collector temperature reaches –40°C. The
display units for vacuum may be selected to be mBar, Pascal (Pa)
or Torr and the temperature may be displayed as °F or °C. The run
time of the refrigeration system and the vacuum pump may be
monitored.

To configure your Freeze Dryer, turn the power switch ON and

press MENU. The display will show:

VACUUM PUMP START UP

MANUAL AUTO

• Either MANUAL or AUTO will be flashing.

• Press SELECT until the desired function is flashing.
• Press MENU.
• The display will show:

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Chapter 4: Using Your Freeze Dryer

VACUUM UNITS

mBar Pa Torr

• Press SELECT until the desired units are flashing.

• Press MENU.
• The display will show:

COLLECT TEMP UNITS

°C °F

• Press SELECT until the desired units are flashing.

• Press MENU.
• The display will show:

REFRIG TOTAL HOUR: XXXX

SERVICE HOUR: XXXX

• To reset the SERVICE HOUR to 0 press SELECT. This

allows you to keep track of the time the refrigeration
system operated since it was serviced.
• The display will show the refrigeration system hours.

REFRIG TOTAL HOUR: XXXX

RESET SERVICE HOUR: ?

• To reset the service hours to 0, continue to hold SELECT 5

seconds until hours are reset.
• TOTAL HOUR cannot be reset.
• Press SELECT.
• The display will show the vacuum pump hours.

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 Chapter 4: Using Your Freeze Dryer

VACUUM TOTAL HOUR: XXXX

SERVICE HOUR: XXXX

• To reset the SERVICE HOUR to 0, press SELECT.

• The display will show:

VACUUM TOTAL HOUR: XXXX

RESET SERVICE HOUR: ?

• If you want to reset the service hours to 0, continue to hold

SELECT five seconds until hours are reset.

Automatic Start-Up
Turn the MAIN POWER switch ON. The refrigeration system
will start and the LCD display will illuminate and read:

AUTO VACUUM HI mBar

WAIT COLLECTOR: XX °C

If the Freeze Dryer was configured in the set up steps above to run
AUTO, the vacuum pump will automatically start when the
collector temperature reaches –40°C. At that point the display will
read:

AUTO VACUUM XXX mBar

RUN COLLECTOR: -40°C

The LCD display will show the actual temperature of the collector.
When the vacuum in the system is above 5 mBar, the vacuum
display will indicate “HI.” At 5 mBar and below, the LCD display
will show a value for the actual vacuum.

Vacuum readings at high values are intended to be used solely as

an indication of how the vacuum system is operating. The vacuum
sensor is calibrated at the low end of the normal freeze drying
range.

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Chapter 4: Using Your Freeze Dryer

Manual Start-Up
Turn the MAIN POWER switch ON. The refrigeration system
will start and the LCD display will illuminate and read:

If the Freeze Dryer was configured in the set up steps above to run
MANUAL, the vacuum pump must be started manually by
pressing the vacuum switch.

MANU VAC HI mBar

WAIT COLLECTOR: XXX

When the collector temperature reaches –40°C the display will

read:

MANU VAC HI mBar

READY COLLECTOR: -40°C

Press the vacuum switch. The vacuum pump will start and a
typical display reading will be:

MANU VAC 0.050 mBar

RUN COLLECTOR: -50°C

The LCD display will show the actual temperature of the collector.
When the vacuum in the system is above 5 mBar, the vacuum
display will indicate “HI”. At 5 mBar and below, the LCD display
will show a value for the actual vacuum.

Vacuum readings at high values are intended to be used solely as

an indication of how the vacuum system is operating. The vacuum
sensor is calibrated at the low end of the normal freeze drying
range.

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 Chapter 4: Using Your Freeze Dryer

Pre-Freezing Samples
Appropriate containers for freeze drying include ampules, serum
bottles, and wide mouth freeze drying flasks. Shell freezing of
samples is recommended for wide mouth freeze drying flasks.
Smaller samples in ampules and serum bottles may be frozen in a
freezer. The sample container size should always be at least two to
three times the sample size (i.e., 40 ml samples should be prepared
in 80 ml containers or larger). The temperature required for pre-
freezing is dependent on the characteristics of the sample. Pre-
freezing temperature typically is at least 10° to 20°C below the
eutectic or collapse temperature of the sample.

Adding Sample
The following procedure should be followed when using sample
valves in the freeze dry process:

1. Pre-freeze samples. Appropriate containers for freeze drying include

ampules, serum bottles, and wide mouth freeze drying flasks. Shell
freezing of samples is recommended for wide mouth freeze drying
flasks. Smaller samples in ampules and serum bottles may be frozen
in a freezer or in the center of the collector chamber. The sample
container size should always be at least two to three times the sample
size (i.e., 40 ml samples should be prepared in 80 ml containers or
larger).
2. Connect a pre-frozen sample to a sample valve on the drying chamber
or manifold using an adapter. Turn the plastic valve knob to the
“VACUUM” position to open the valve, which connects the attached
sample to system vacuum. The bevel on the knob should be
positioned toward the sample port to apply vacuum to the sample.
Bevel in “vent” position
Vent/Backfill Port

Bevel in “vacuum”
position

Adapter

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Chapter 4: Using Your Freeze Dryer

3. Before adding another sample, allow system vacuum to return

to 0.133 mBar or lower. Any combination of valves and
sample sizes may be utilized at one time provided that the
system vacuum and collector temperature remain sufficiently
low to prevent melting of the frozen sample.
4. When all the frost has disappeared from the outer surface of the
sample container and no cold spots can be detected by handling
the container, the sample is nearly dry. To be certain of low
final moisture content, dry the sample for several hours past
this point.
5. To remove a container after drying is complete, turn the plastic
knob on the valve to the “VENT” position, which closes the
valve and vents the container. Should backfilling with an inert
gas be required, connect the gas supply line to the vent port on
the valve before turning the plastic knob on the valve to vent
position. The sample container may now be removed. In the
vent position the bevel on the knob should point away from the
sample port.
6. Ampules may be flame sealed while connected to a valve by
using a sealing torch. Care must be taken not to burn the valve.
An insulation material placed between the valve and the torch
is recommended.

Shut Down
At the end of a run or when a sufficient amount of condensate
accumulates on the collector coil to obstruct the flow of vapor to
the collector chamber, the Freeze Dryer should be defrosted. First,
release system vacuum by turning the plastic knob on a valve to
the open position or by pulling the collector chamber drain plug
out of the drain hose. Now press the Vacuum Switch on the
control panel to turn the vacuum pump OFF. Turn OFF the Main
Power Switch on the right hand side of the cabinet.

Defrosting
The following procedure should be followed when defrosting the
collector coil:

1. Pull the collector chamber drain hose out from the left hand
side of the Freeze Dryer and remove the drain plug. Place the
drain hose in a suitable container to collect the condensate that
will melt off the collector coil.
2. Remove the collector chamber lid and allow ambient room air
to melt the ice. Dispose of the liquid appropriately.
3. Flush the collector chamber with water and wipe chamber dry.

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 Chapter 4: Using Your Freeze Dryer

4. If rapid defrost is desired, pour warm water over the collector

coil. Do not allow the liquid to enter the vacuum port on the
upper rear of the chamber.
5. Reinstall the drain hose plug and slide drain hose back into the
side of the cabinet. Dispose of the liquid appropriately.

Utilization of acid requires immediate cleaning

! and neutralization after defrost or physical
damage to the collector chamber and collector
coil will result.

Do not attempt to chip ice from the collector coil

as damage may occur to the coil.

Never attempt to start the vacuum pump when

there is liquid in the collector chamber. This
could result in damage to the vacuum pump.

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 CHAPTER 5
MAINTAINING YOUR
FREEZE DRYER
Under normal operation, the Freeze Dryer requires little
maintenance. The following maintenance schedule is
recommended:

As needed:

1. Clean up all spills; remove liquids from the chamber.

2. Clean lid and gasket using soft cloth, sponge or chamois and a
mild, non-abrasive soap or detergent.
3. Check oil level of the vacuum pump. It should be between
MIN and MAX. If the oil level is less than an inch (25.4 mm)
above MIN, add oil to proper level.
4. If oil shows cloudiness, particles or discoloration, drain the
pump and replace with fresh oil.
5. Utilization of acids requires immediate cleaning and
neutralization after a run or physical damage to the
collector chamber and collector coil will result.
6. Check the collection chamber for condensed or frozen solvents
and dispose of appropriately. Completely empty the collector
chamber before the next run or before attempting to start the
vacuum pump.
7. If sample valves leak vacuum or the stem becomes difficult to
turn, pull the stem out of the rubber valve body and apply a
thin coat of vacuum grease to the stem. Reinsert the stem into
the valve body.

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 Chapter 5: Maintaining Your Freeze Dryer

Monthly:

1. The rubber components on the Freeze Dryer may eventually

deteriorate and require replacement. The effective life of
rubber parts depends upon both their usage and the surrounding
environment. Check all rubber hoses and gaskets and replace
any that show signs of hardening, permanent set or
deterioration.
2. Using a soft cloth, sponge or chamois and a mild, non-abrasive
soap or detergent, clean the acrylic chamber lid.
3. Using a soft cloth, sponge, or chamois and a mild, non-abrasive
soap or detergent, clean the exterior surfaces of the Freeze
Dryer. Liquid spray cleaners and polishes may be used on the
exterior surfaces. Do not use solvents to remove stains from
the exterior surfaces as they may damage the finish.

Annually:

1. Every 12 months, or more often if the Freeze Dryer is operated

in a dusty environment, the refrigeration system condenser
should be cleaned. Using a vacuum cleaner with brush
attachment, clean the condenser to ensure proper airflow for
peak performance.

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 CHAPTER 6
TROUBLESHOOTING
Refer to the following if your Freeze Dryer fails to operate
properly. If the suggested corrective actions do not solve your
problem, contact Labconco for additional assistance.

Labconco Freeze Dry Systems that are clean, dry and without
samples attached should reach a vacuum of 0.133 mBar within 10
minutes and should achieve an ultimate vacuum of 0.033 mBar
within 18 hours when the refrigeration is operating. If the Freeze
Dryer does not obtain a satisfactory vacuum, perform the following
maintenance tests.

I. Vacuum Pump
First make sure that the vacuum pump operates. If it fails to
operate, check the electrical connections of the Freeze Dryer to the
power source and then check the electrical connection of the
vacuum pump to the Freeze Dryer. If the vacuum pump has a
power switch, make sure that it is turned on. If the vacuum is not
adequate when the vacuum pump is operating, proceed with the
following steps:

1. Check the oil and ensure it is clear and clean. If the oil
looks cloudy or has any particulates, replace the pump oil.
Sometimes it may be necessary to flush the pump with
clean oil several times. To flush the pump, run the pump 5
to 10 minutes to allow the oil to warm up. Drain the oil
and refill with clean oil. Repeat as necessary.
2. Check the oil level in the pump. Ensure it is filled to the
correct level.
3. Check vacuum hose connections from the pump to the
Freeze Dryer and try running the unit

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 Chapter 6: Troubleshooting

If vacuum problems continue, consider obtaining a second

vacuum gauge capable of reading a vacuum of 0.010 mBar.
It is often useful in determining if the vacuum pump is
operating properly and the vacuum sensor reading is
accurate.

4. Isolate the pump by disconnecting the vacuum hose from

the Freeze Dryer. Deadhead the pump by inserting the
vacuum sensor from a secondary vacuum gauge into the
end of the vacuum hose and observe the vacuum reading
obtained. Confirm that the pump is capable of achieving an
ultimate vacuum less than 0.010 mBar or approximately 10
microns. If an inadequate vacuum reading is obtained, the
pump has most likely failed and may need to be replaced or
rebuilt.

II. Gaskets, Tubing, Connections, Sample

Valves
1. Check all sample valves on the drying chamber/manifold
and ensure all valves are closed or in the vent position.
Check to ensure all fittings are tight and sealed properly
against the chamber. Inspect valves for cracks or cuts.
2. Inspect gasket(s) for cracks or defects. Ensure the gasket is
clean. Apply a thin coat of vacuum grease to the gasket.
Keep in mind too much vacuum grease can actually cause
leaks.
3. Check the drain line and ensure the drain plug is installed
and the tube appears to be in good condition.
4. Check all rubber vacuum tubing for signs of deterioration
or cracking.
5. Check all connections and make sure they are secure and
leak tight.
6. When checking the sample valves, pull as much vacuum as
possible. If a vacuum indication is displayed, wiggle or
rotate the valves and watch the gauge for any fluctuations.
Fluctuations can indicate a potential vacuum leak. If the
valve seems to be in good condition, remove the valve and
apply a thin coat of vacuum grease to the stem and the
outside sealing surface of the valve body, and reinstall the
valve. DO NOT APPLY VACUUM GREASE TO THE
SAMPLE VALVE GASKETS, WHICH SEAL TO THE
WALL OF THE DRYING CHAMBER. If the valve still
seems to be the source of the problem, remove the valve

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Chapter 6: Troubleshooting

and stopper the hole with a rubber stopper. Continue

checking the other valves.
7. Make sure that the sample valve bulkhead nuts are tight.
ither tighten by hand or use the wrench supplied with
chambers. To use the wrench, first remove the knob and
stem assembly and also the valve body. The pins on the
wrench fit into the round holes of the nut. The nut should
be snug, but avoid over-tightening. Replace the valve
body, knob and stem.

The illustrations below show how the sample valve installs on a

chamber and a manifold.

Valve Body

Knob & Stem

Assembly

Manifold Assembly Valve Body Bulkhead Nut

Knob & Stem Valve Body Trim Ring Knob & Stem Gasket
Thrust Washer Bulkhead
Assembly Assembly (No grease Fitting
required)
Valve Assembly (Manifold)
Valve Assembly (Stainless Steel Chamber)

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 Chapter 6: Troubleshooting

III. System Components and Collection

Chamber Isolation
This test determines if the source of a leak is in the drying
chamber.

1. Remove the drying chamber or manifold.

2. Leave the gasket on and turn a large freeze dry flask upside
down to cover the connection port.
3. Start the Freeze Dryer and observe the vacuum indication.
If the vacuum indication is good, then the problem is in the
drying chamber or manifold and you should return to
Section II, Gaskets, Tubing, Connections, Sample Valves.

Freeze Dry Flask

Gasket

Isolating the freeze dry system from the accessory

chamber/manifold

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Chapter 6: Troubleshooting

The following test determines if a vacuum leak is in the collection

chamber.

1. Insert a rubber stopper in the small hole in the rear of the

collector chamber.
2. Start the Freeze Dryer and observe the vacuum indication.
If the vacuum is good, the problem is in the collection
chamber. If it is bad, the problem is in the plumbing
connections from the collection chamber to the vacuum
pump.
3. If the leak is in the chamber, check the drain hose, hose
connections and the drain plug.
4. If the leak is in the plumbing connections, check all rubber
parts, fittings and clamps.

Stopper

Checking the connections to the pump and the vacuum tube

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 Chapter 6: Troubleshooting

Refrigeration Module Operation

Under a no-load condition, the FreeZone Freeze Dry System can
achieve a collector temperature of –47°C or lower when the
vacuum pump is operating, depending on ambient temperature and
humidity. If the collector temperature does not reach –47°C within
40 minutes, then the refrigeration module is not functioning
properly.

If any repairs are required on the refrigeration module, contact

your local laboratory supply dealer. If your laboratory supply
dealer is unable to provide assistance, please call Labconco.
Repairs should only be undertaken by a competent refrigeration
technician or through an authorized Labconco service agency.

Product Service: Domestic 1-800-522-7658, International 816-333-8811 31

 CHAPTER 7
MODIFYING YOUR
FREEZE DRYER
DISPLAY
Your Freeze Dryer has been carefully calibrated and tested before
shipping, however under certain circumstances it may be necessary
to adjust the calibration of the temperature and/or vacuum display.
Adjustments may be necessary if the Freeze Dryer requires service.

Temperature and Vacuum Display

An offset may be added to the collector temperature or vacuum
display. Use an accurate traceable reference to compare the
readings.

Temperature offset may be varied ±5° in 1°C increments.

Vacuum offset may be varied ±0.010 mBar in 0.001 mBar
increments.

Modifying the Display

Master Reset
If you want to restore all factory settings hold MENU
button while turning on the power switch.
Release the MENU button when the firmware revision
message appears.

This will reset:

Vacuum units to mBar

Vacuum offset to 0 mBar
Temperature units to ^C

Temperature offset to 0^C

Refrigeration total hours to 0

32 Product Service Domestic 1-800-522-7658, International 816-333-8811

 Chapter 7: Modifying Your Freeze Dryer Display

Vacuum pump total hours to 0

Refrigeration to operate in Manual Mode

Temperature and Vacuum Offsets

If you do not want to restore all factory settings but want to

adjust offsets, press MENU and hold for approximately 10
seconds. The display will show

TEMPERATURE OFFSET: 0
VACUUM OFFSET: 0

Press SELECT until the appropriate temperature offset is

shown. Each number represents 1°C.
Press VACUUM until the appropriate vacuum offset is
shown. Each number represents 0.001 mBar.
Press MENU to lock in the offsets and to return to the main
screen.

Product Service: Domestic 1-800-522-7658, International 816-333-8811 33

 APPENDIX A
FREEZE DRYER
COMPONENTS
The following pages list components that are available for your
Freeze Dryer. The parts shown are the most common replacement
parts. If other parts are required, contact Product Service.

34 Product Service Domestic 1-800-522-7658, International 816-333-8811

 Appendix A: Freeze Dryer Components

Item Part No. Description

1 7395800 Lid Gasket
2 7395801 Lid
3 7435600 Back Panel Assembly, 115V
7435700 Back Panel Assembly, 230V
4 7953800 Motor Fan, 115V
7953801 Motor Fan, 230V
5 7437700 Compressor, 115V, 60 Hz
7437701 Compressor, 230V, 50 Hz
7437702 Compressor, 230V, 60 Hz
6 1302300 Switch
7 7426500 Control Panel Label
8 7438000 Printed Circuit Board
9 7728000 Drain Plug
10 1643600 O-Ring
11 7645904 Drain Hose, Collector Chamber
12 7646000 Vacuum Hose, Vacuum Pump (Not Shown)
13 1336400 Power Cord 115V (Not Shown)
1342100 Power Cord, 230V (Not Shown)
14 7445400 Vacuum Sensor (Not Shown)
15 7515300 Temperature Sensor, Collector (Not Shown)
16 7438100 Wire Harness, 115V (Not Shown)
7438200 Wire Harness, 230V (Not Shown)

Product Service: Domestic 1-800-522-7658, International 816-333-8811 35

Appendix A: Freeze Dryer Components

8
9
10

11
6

36 Product Service: Domestic 1-800-522-7658, International 816-333-8811

APPENDIX B
FREEZE DRYER
DIMENSIONS

Product Service Domestic 1-800-522-7658, International 816-333-8811 37

 APPENDIX C
FREEZE DRYER
SPECIFICATIONS
This Appendix contains technical information about the Freeze
Dryer including electrical specifications, environmental operating
conditions and wiring diagrams.

Electrical Specifications

Catalog # Voltage Frequency Phase Amperage

Nominal Operating Nominal Max. with
Range w/o Vacuum
Vacuum Pump
Pump
7740020 115V 103-127 60 1 6 14
100V 90-110 50 1 6 14
7740021 115V 103-127 60 1 6 14
100V 90-110 50 1 6 14
7740030 230V 198-254 50 1 2.5 7
7740031 230V 198-254 50 1 2.5 7
7740040 230V 187-253 60 1 2.5 7
7740041 230V 187-253 60 1 2.5 7

Environmental Conditions
• Indoor use only.
• Maximum altitude: 6562 feet (2000 meters).
• Ambient temperature range: 41° to 104°F (5° to 40°C).
• Maximum relative humidity: 80% for temperatures up to
88°F (31°C), decreasing linearly to 50% relative humidity
at 104°F (40°C).
• Main supply voltage fluctuations not to exceed ±10% of the
nominal voltage.
• Transient overvoltages according to Installation Categories
II (Overvoltage Categories per IEC 1010). Temporary
voltage spikes on the AC input line that may be as high as
1500V for 115V models and 2500V for 230V models are
allowed.
• Used in an environment of Pollution degrees 2 (i.e., where
normally only non-conductive atmospheres are present).
Occasionally, however, a temporary conductivity caused by
condensation must be expected, in accordance with IEC
664.

38 Product Service Domestic 1-800-522-7658, International 816-333-8811

 Appendix C: Freeze Dryer Specifications

Wiring Diagram (115V, 60 Hz Models)

Product Service: Domestic 1-800-522-7658, International 816-333-8811 39

Appendix C: Freeze Dryer Specifications

Wiring Diagram (230V, 50 or 60 Hz Models)

40 Product Service: Domestic 1-800-522-7658, International 816-333-8811

 APPENDIX D
FREEZE DRYER
ACCESSORIES
The following accessories are available for the Freeze Dryer.

PART # DESCRIPTION
1472100 Vacuum Pump
Two stage direct drive pump, 117 liters/minute. 115 VAC,
50/60 Hz, single phase, 4.6 amps. Includes Pump Exhaust Filter
1473400. (Cat. # 1473400)

7739402 Vacuum Pump

Two stage direct drive pump, 117 liters/minute. 230 VAC,
50/60 Hz, single phase, 2.4 amps. Includes Pump Exhaust Filter
1473400. (Cat. #1473400)

7438700 Vacuum Pump

Two stage direct drive pump, 108 liters/minute. 115 VAC,
50/60 Hz, single phase. Includes Pump Exhaust Filter.
(Cat. # 7670400)

7438800 Vacuum Pump

Two stage direct drive pump, 108 liters/minute. 230 VAC,
50/60 Hz, single phase. Includes Pump Exhaust Filter.
(Cat. # 7670400)

7769600 Vacuum Pump Chemical Resistant

Two stage direct drive pump, 173 liters/minute. 115 VAC,
50/60 Hz, single phase. Includes Pump Exhaust Filter.
(Cat. # 7670400)

7769800 Vacuum Pump chemical Resistant

Two stage direct drive pump, 173 liters/minute. 230 VAC,
50/60 Hz, single phase. Includes Pump Exhaust Filter. (Cat. #
7670400)

Product Service Domestic 1-800-522-7658, International 816-333-8811

41
Appendix D: Freeze Dryer Accessories

PART # DESCRIPTION
1472200 Pump Inlet Filter
Disposable filter that prevents oil back streaming and protects
vacuum pump from submicron particles. Fits vacuum pumps
1472100 and 7739402.

1473400 Pump Exhaust Filter

Disposable filter that removes visible oil mist and odor from
vacuum pump exhaust. Fits vacuum pumps 1472100 and
7739402.

1473200 Replacement Element, Oil Mist, Pump Exhaust Filter

Fits pump exhaust filter 1473400.

1473300 Replacement Element, Odor, Pump Exhaust Filter,

package of 5
Fits pump exhaust filter 1473400 or vacuum pumps 1472100 and
7739402.

7438600 Replacement Filter – Element

Fits vacuum pumps 7438700 and 7438800

1988000 Vacuum Pump Oil, 1 Liter

A molecularly distilled hydrocarbon oil with low vapor pressure.
For vacuum pumps 1472100 and 7739402

7772000 Soda Acid Trap

Secondary trap that prevents migration of corrosive chemicals into
vacuum pump.

7772100 Replacement Media for Soda Acid Trap

7772700 Vacuum Pump Oil, 1 Liter

A mineral oil with low vapor pressure.
For vacuum pumps 7438700, 7438800, 7769600, 7769800

42 Product Service: Domestic 1-800-522-7658, International 816-333-8811

 Appendix D: Freeze Dryer Accessories

PART # DESCRIPTION
7772500 Carbon Solvent Trap
Secondary trap that prevents migration of organic solvents into
vacuum pump.

7772600 Replacement Activated Carbon Media for Carbon Solvent

Trap

7538000 Secondary Vacuum cold Trap

Provides additional protection for the vacuum pump when
processing low eutectic samples 9-3/4" high x 7-7/8" diameter,
and 304 stainless steel with 3/4" vacuum connections. For use with
dry ice & solvent. Cools to approximately –75°C

7522800 12 Port Drying Chamber

9 ¾" high x 7 7/8" diameter, 304 stainless steel with 12 freeze dry
valves.

7521000 Heated Drying Chamber

10 ½" high x 9" diameter type 304 stainless steel. The 3 shelf
product heaters heat to 43°C (110°F). 115V

7521001 Heated Drying Chamber

10 ½" high x 9" diameter type 304 stainless steel. The 3 shelf
product heaters heat to 43°C (110°F). 230V

7509200 Product Heater

For use in chamber 7522800. Provides 3 heated shelves operating
at 43°C (110°F). 115V

7509201 Product Heater

For use in chamber 7522800. Provides 3 heated shelves operating
at 43°C (110°F). 230V

7443500 Clear Drying Chamber with 8 Valves

For bulk drying or drying in flasks
7442100 Drying Rack – Unheated
Provides 3 shelves. For use in chambers 7443500 and 7522800
7442400 Clear Drying Chamber
For bulk drying

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Appendix D: Freeze Dryer Accessories

PART # DESCRIPTION
7522300 20 Port Manifold
10" high x 27 ½" wide x 8 5/8" deep. Type 304 stainless steel
manifold with 20 freeze dry valves.

7868500 48 Port Ampule Manifold

28 5/8" high x 5" wide x 5" deep. Type 304 stainless steel with
48 each ¼" tube stems.

7509600 Sample Valve Kit

Includes neoprene valve body, knob and installation parts.

7522900 16 Port Chamber

13" high x 138" diameter, 304 stainless steel with 16 freeze dry
valves.

44 Product Service: Domestic 1-800-522-7658, International 816-333-8811

 Appendix D: Freeze Dryer Accessories

Fast-Freeze® Flasks
Variety of adapters Compatible with
available. You may all major brands of
select from glass or laboratory freeze dry
stainless steel apparatus.
adapters, available
straight or with 45° No complex filter
bend. (Required — retainer is necessary.
order separately.) For those who use
filters, they are easily
Top seals easily. The inserted between the
flexible silicone rubber adapter and top of
top snaps on and off the flask. One hun-
easily, yet provides a dred filters are sup-
reliable, high vacuum plied with each flask.
seal.

Only two pieces

per flask. Wide mouth
The complete opening loads fast,
Fast-Freeze Flask cleans easily.
has only two
components:
• a high strength Horizontal shell
borosilicate freezing. Flask
glass bottom may be placed in
to withstand a horizontal posi-
extreme tion and rotated
temperatures in a bath for shell
Fast-Freeze Flasks are specially designed to be easier to and high freezing.
vacuum.
handle, faster to load and more convenient to use than other
freeze dry glassware now in your laboratory. Compatible • a flexible, non-
contaminating
silicone rubber Available in nine
with all major brands of laboratory freeze dry equipment, convenient sizes.
top.
Fast-Freeze Flasks eliminate the risk of contamination from No vacuum
vacuum grease, reduce spillage of valuable samples and grease is
require no washers, gaskets or retainers. necessary. Flat bottom
No threads, reduces spills,
hooks or springs provides better
How to select Fast-Freeze Flasks for are needed. balance.
your Freeze Dry System Entire flask may
be autoclaved.
Select Fast-Freeze Flasks based on your sample sizes. Flasks
should be filled no more than one-third of their volume so
that maximum surface area is achieved and efficient Adapters
lyophilization is assured. A complete Fast-Freeze Flask Add the Adapters for connecting
includes a rubber top, glass bottom and a supply of filter the Fast-Freeze Flasks to the valve
paper. Tops, bottoms and filter paper are available separate- ports on your drying chamber or
ly as replacement components. Adapters are required to manifold. Choose borosilicate glass
attach flasks to freeze dry valve ports. or stainless steel adapters in 1/2"
and 3/4" diameters.
Flask Complete Flask Flask Flask Top Dimensions/
Size Flask Bottom Top Adapter Flask Bottom Diameter Straight 45° Bend
Diameter* HxD Adapter Adapter
40 ml 7540000 7542000 7544000 1/2" 76 mm x 34 mm Borosilicate Glass
80 ml 7540200 7542200 7544000 1/2" 115 mm x 34 mm 1/2" flask top to 1/2" valve* 7545000 7545600
120 ml 7540300 7542300 75442-00 3/4" 68 mm x 59.2 mm 3/4" flask top to 3/4" valve* 7545200 7545800
150 ml 7540400 7542400 7544200 3/4" 85 mm x 59.2 mm 1/2" flask top to 3/4" valve* 7545400 7546000
300 ml 7540600 7542600 7544200 3/4" 145 mm x 59.2 mm 3/4" flask top to 1/2" valve* 7545400 7546000
600 ml 7540800 7542800 7544400 3/4" 135 mm x 90.2 mm Stainless Steel
900 ml 7540900 7542900 7544400 3/4" 190 mm x 90.2 mm 1/2" flask top to 1/2" valve* 7547000 7547400
1200 ml 7541000 7543000 7544400 3/4" 240 mm x 90.2 mm 3/4" flask top to 3/4" valve* 7547200 7547600
2000 ml 7541200 7543200 7544400 3/4" 380 mm x 90.2 mm
7544810 Replacement Filter Paper
*Valves on the freeze dry systems, drying chambers and manifolds offered in this catalog fit adapters
of both 1/2” and 3/4" sizes. Size information is provided for selecting adapters for older Labconco mod- 1000 (10 packages of 100). 1" diameter (28mm). Pore size 17 microns.
els and other freeze dryers. Shipping weight 0.5 lb.

Product Service Domestic 1-800-522-7658, International 816-333-8811 45

 Appendix D: Freeze Dryer Accessories

Lyph-Lock® Flasks
Borosilicate glass Compatible with
adapters with all major
either 19/38 or 24/40 brands of labo-
standard taper joints ratory freeze
have 90° bend. dry apparatus.
(Required — order
separately.)

Only three pieces

per flask — no
hooks or springs. Silicone rubber
The complete seal grasps top
Lyph-Lock Flask and bottom.
includes: The flexible ring
• a flask top of holds securely
high strength together during
borosilicate pre-freezing
glass and lyophiliza-
• a flexible, non- tion.
contaminating
silicone rubber
seal
• a flask bottom
of borosilicate
glass to with-
stand extreme
temperatures
and high
Lyph-Lock Flasks simplify your lyophilization procedures vacuum.
because they have only three pieces per flask — a high
strength borosilicate glass top and bottom with a silicone Available
in seven
rubber ring seal. The unique wide-mouth design helps you convenient sizes. Flat bottom
load samples easily and lyophilize efficiently. reduces spills,
Entire flask may provides better
be autoclaved. balance.
How to select Lyph-Lock Flasks for
your Freeze Dry System Provides a high
vacuum seal for
Select the Lyph-Lock Flasks based on your sample sizes. efficient
lyophilization.
Flasks should be filled to no more than one-third of their
volume so that maximum surface area is achieved and effi-
cient lyophilization is ensured. A complete Lyph-Lock Flask
includes a glass top and bottom and a rubber ring seal. Tops,
bottoms and seals are available separately as replacement Adapters
components. Adapters are required to attach flasks to freeze Add the Adapters for connecting the Lyph-Lock Flasks to the
dry valve ports. valve ports on your drying chamber or manifold.

Flask Complete Complete Flask Flask Lyph-Lock Flask Dimensions 90° Bend Description
Size Flask Flask Top Top Seal Bottom Flask Bottom Adapter
19/38 STJ 24/40 STJ 19/38 STJ 20/40 STJ H x ID
7568000 Connects 19/38 STJ Flask Top to 1/2" valve*
25 ml 7550000 7554000 7552000 7556000 75590-00 75570-00 37 mm x 34 mm
50 ml 7550200 7554200 7552000 7556000 7559000 7557200 67 mm x 34 mm 7568200 Connects 19/38 STJ Flask Top to 3/4" valve*

100 ml 7550400 7554400 7552200 7556200 7559200 7557400 50 mm x 59.2 mm 7568400 Connects 24/40 STJ Flask Top to 1/2" valve*
250 ml 7550600 7554600 7552200 7556200 7559200 7557600 110 mm x 59.2 mm
7568600 Connects 24/40 STJ Flask Top to 3/4" valve*
500 ml 7550800 7554800 7552400 7556400 7559400 7557800 103 mm x 90.2 mm
750 ml 7550900 7554900 7552400 7556400 7559400 7557900 145 mm x 90.2 mm *Valves on the freeze dry systems, drying chambers and manifolds offered in this catalog fit adapters
of both 1/2” and 3/4" sizes. Size information is provided for selecting adapters for older Labconco
1000 ml 7551000 7555000 7552400 7556400 7559400 7558000 187 mm x 90.2 mm models and other freeze dryers.

46 Product Service Domestic 1-800-522-7658, International 816-333-8811

Appendix D: Freeze Dryer Accessories

Serum Bottles and Vials

Threaded Vials
Stoppers and Threaded Vials with Screw Caps are supplied
in packages of 200.

Size Vials with Stoppers

Screw Caps
5 ml 7762300 7762200
10 ml 7762600 7762200

Accessories

Perfect for long term storage of freeze dried samples.

Labconco Serum Bottles and Threaded Vials are specifically
designed for lyophilization applications. Their uniform thin
wall construction ensures even freezing and drying. Bottles
and vials are ideal containers for use in the FreeZone
Stoppering Tray Dryer. Serum bottles also connect to valve Seal Crimper
ports on drying chambers and manifolds. Secures tear-away Aluminum Seals.
Serum Bottles 7578000 Seal Crimper for 13 mm corkage.
Serum Bottles, Stoppers and Seals are supplied in pack- Shipping weight 3 lbs. (1.4 kg).
ages of 100. 7578100 Seal Crimper for 20 mm corkage.
Shipping weight 3 lbs. (1.4 kg).

Vacuum Stoppering Adapter

7593000 Adapter connects to valve ports for manual stop-
pering of 20 mm corkage serum bottles under original vacu-
um. The stopper and serum bottle are inserted into the
adapter body and lyophilization begins. After the process is
completed, the researcher slides the stopper into position
so when the valve is turned from vacuum to vent the serum
bottle is tightly sealed by the stopper. The ejector tube is
then used to force the serum bottle out of the adapter.
Size 20 mm 13 mm Split Aluminum Sleeve-Type Shipping weight 1 lb. (0.4 kg).
Corkage Corkage Stoppers Seals Stoppers
2 ml 7575010 7576010 7577010 Stoppering Adapter Body Serum Bottle
3 ml 7575210 7576010 7577010
5 ml 7573010 7576210 7577110 7577510 Valve Port
10 ml 7573210 7576210 7577110 7577510
20 ml 7573410 7576210 7577110 7577510
30 ml 7573610 7576210 7577110 7577510
50 ml 7573810 7576210 7577110 7577510
100 ml 7574010 7576210 7577110 7577510
125 ml 7574210 7576210 7577110 7577510 Vent Hole
Ejector Tube Stopper

Product Service Domestic 1-800-522-7658, International 816-333-8811 47

 Appendix D: Freeze Dryer Accessories

Ampules
Accessories

Oxygen/Natural Gas Sealing Torch

7578500 Torch specifically designed for flame sealing freeze
dry ampules. Seals all types of heat-resistant glass. Connects
to natural gas, butane or propane and oxygen with 1/4" ID
hose connectors. Shipping weight 3 lbs. (1.4 kg).

Labconco Ampules are fabricated of highest quality borosili-

cate glass for strength and durability. They are available in
five different configurations to provide the ideal ampule for
your specific lyophilization requirements, from tray drying to
flame sealing applications. Ampule Valve Adapters
7593401 Adapter connects ampules to valve ports or stainless
Ampules
steel stems. Ten per package. Shipping weight 0.5 lb. (0.2 kg).
Ampules are supplied in packages of 100.

Stoppers
7572510 Stopper for Stoppering Ampules 75723-10.
100 per package. Shipping weight 3 lbs. (1.4 kg).

Size Straight Bulb-Type Pre-Scored Round Stoppering

Flat Bottom Bottom
1 ml 7572710 7570010 7570610
2 ml 7570810 7572310
Trident Adapter
4 ml 7572810
7762700 Three Way Adapter permits attachment of three
5 ml 7570210 7571010
ampules to a single valve port. Ampules are attached using
10 ml 7571210 7571610 Ampule Valve Adapters or 1/8" surgical tubing (not provided).
25 ml 7571810 Cavity in adapter body can be filled with cotton fiber media
50 ml 7572010 to help prevent contamination between samples.
Shipping weight 3 oz. (0.1 kg).

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